FastQCFastQC Report
Tue 16 Jun 2015
000000000-AFC60 l01n02.3320000000c8f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFC60 l01n02.3320000000c8f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23229140
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCGCTGTA15306166.589206488057672No Hit
CTAACCTCCGCT13494685.809375637668893No Hit
CAGGCGTATTGG10475304.509551365224886No Hit
TCCGACACAATT9236373.9761997215566307No Hit
GCTGATGAGCTG9226373.971894783879214No Hit
ACACCTGGTGAT9149203.9386735798225847No Hit
GCACACACGTTA8999333.874155478851133No Hit
TACACGATCTAC8892753.8282734530852194No Hit
CCTCGTTCGACT8582543.694729981394059No Hit
AGCTGTTGTTTG8506713.6620856389862046No Hit
TGAGTCACTGGT8484503.6525243724046605No Hit
AATCAGTCTCGT8204413.531947372997881No Hit
GAACCAAAGGAT7575873.2613648202214978No Hit
GGAGACAAGGGA7446593.205710585927848No Hit
TTACTGTGCGAT7423703.1958565835842396No Hit
CCAGTGTATGCA7347503.16305295848232No Hit
AATCCGTACAGC7302893.143848631503362No Hit
AGGCTACACGAC7149823.0779529504751357No Hit
GTACATACCGGT7092023.0530704106996644No Hit
GACTTGGTATTC6894642.9680995508228025No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGCG4100.06.00000051
GCGCGGT2050.06.00000055
GACACCG1451.9645086E-106.00000054
GGACTCC806.540038E-56.00000051
ATTGTCA806.540038E-56.00000055
GCGCCGC1202.5700501E-86.00000055
TACGGCT600.0033967636.00000056
CGTTCGG3200.06.00000054
CACCTAG4100.06.00000052
ATTCCGG4100.06.00000055
GGGCGAT1202.5700501E-86.00000056
CAGGCCT1601.0913936E-116.00000051
AGCGCCG806.540038E-56.00000054
GGTCTGT600.0033967636.00000055
ATAGTGG1601.0913936E-116.00000055
CGCAATT2400.06.00000056
AGGCTGC806.540038E-56.00000051
CGGGTGA1303.6470738E-96.05
TTAGGGT550.0091611636.06
CGGCGTC953.430323E-66.02
Produced by FastQC (version 0.11.2)