FastQCFastQC Report
Tue 16 Jun 2015
000000000-AFC60 l01n01.3310000000c8f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFC60 l01n01.3310000000c8f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23229140
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG420798418.115108867568924No Hit
AGCTCTGCGAGTTTGCTCCCATATTGTTGCAGTTAAAACGCCCGTAGTCT15383106.622328678547721No Hit
AGCTCTGCGAGTTTGCTCCCATATTGTTGCAGTTAAAACGCTCGTAGTCA13426215.779899729391618No Hit
AGCTCCAAAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG12865425.538483129379736No Hit
AGCTCCAATAGCGTATACTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG10172904.379370049859788No Hit
AGCTCTCTAAGTGTAAATCGTTATTGTTGCGGTTAAAAAGCTCGTAGTTG8365963.6014936411765563No Hit
AGCTCTCATGGCGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG7633933.286359288376582No Hit
AGCTCCAAGAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG5123412.2055960745856282No Hit
AGCTCCAATAAGGAAAACTAAAGTTGCTGTGTTTAAAAAGCTCGTAGTTG3494691.5044422651893268No Hit
AACTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG3434881.4786944329406944No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG2946921.2686306940334424No Hit
AGCTCTCATACTGCATTGAATTGCTGTTGCGGTTAAAAAGTTCGTAGTTG2525051.0870182882362411No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCGGTTAAAAAGCTCGTAGTTG2404921.0353030719174279No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG2223170.9570608296303694No Hit
AGCTCCAATAGCGTATACTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG2150910.9259533499733524No Hit
AGCTCTCAATGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG2005480.8633466413306735No Hit
AGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG1968940.8476163990573907No Hit
AGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1953440.840943745657394No Hit
ACCATCATCAATCAAGAACGAAAGTGCCTGACTGACTGTATGCGCTGTAA1802430.7759348817907163No Hit
AGCTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG1674540.7208790338342272No Hit
AGCTCTCCTAGTGTATCTCGTTATTGCTGCGGTTAAAAAGCTCGTAGTTG1216480.5236870585824529No Hit
ACCATCATCAATCAAGAACGAAAGTGCCTGACTGACTCTAACCTCCGCTA1213260.5223008686503245No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG1150100.49511088227975725No Hit
AACTCCAAGAGTGTATCTTAATGTTGCTGCAGTTAAAAAGCTCGTAGTTG1147540.4940088182343384No Hit
AGCTCCAAGAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1131260.4870003796955032No Hit
AGCTCCAATAGCTTTGTTGAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG1099240.47321596925241316No Hit
AGCTCTAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1088460.46857524643615733No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG1048250.45126509203526255No Hit
TAATCGTCGAGTTTGACATCCAATTATTGCGATTAAAACGTTCGTAGTTG1029430.4431631993263634No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1020500.4393188899804297No Hit
AGCTCTAATAGCGTATATTAATGTTGCTGCAGTTAAAAAGCTCGTAGTTG1003500.4320004959288204No Hit
AGCTCCGCCAGTGTATATCTATGCTGCTGCGGTTAAAAAGCTCGTAGTTG978520.4212467616106321No Hit
AGCTCCAATAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG836830.3602500996593072No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTGAAAAGCTCGTAGTCG797340.34324990077118656No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG733470.31575426382552263No Hit
TCCATCTCTCGGGGGATTCTCCTCACATTTGCTCAATCAAGAACGAAAGT708230.3048886011277215No Hit
AGCTCCGCAGGTGTATATCTATGCTGCTGCGGTTAAAAAGCTCGTAGTTG638180.27473251269741367No Hit
AGCTCTGATAGTATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG633570.27274793642812434No Hit
TTGCAGCGAGCTGGCGGTTTGGTTACCAGCGCCTGGCTGATAGGCGACAA611820.2633846969797418No Hit
AGCTCCGCAAGTGTATTCCTATACTGCTGCGTTTAAATAGCTCGTAGTTG609600.2624290008153552No Hit
AGCAGTTTATGTCGATTTCAGTCCATTTTCTGAATACAAATGTGTCCCTA602540.25938971481509865No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG582490.25075831477187704No Hit
ACCATCATCAATCAAGAACGAAAGTGCCTGACTGACTGAACCAAAGGATA578920.24922145202103907No Hit
AGCTCTGCAAGTTTGCTCCCATATTGTTGTAGTTAAAACGCTCGTAGTCT554730.23880780777936678No Hit
AGCTCCAATAGCGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG538150.23167022111020896No Hit
AGCTCTAATAGTATATACGAATCTTGCTGCGGTTAAAAAGCTCGTAGTTG524450.2257724564921474No Hit
AGCTCCAAAAGCGTATATTAACGCTGCTGCTGTTAAAGGGTTCGTAGTTG487570.2098958463378326No Hit
ACCATCATCAATCAAGAACGAAAGTGCCTGACTGACTTACACGATCTACA482350.20764866887022074No Hit
AGCTCCTCTAGTGTACATCGATATTGCTGCGGTTAAAAAGCTCGTAGTTT454870.19581870013267816No Hit
AGCTCTTTTAGTGTATATTAAATTTGTTGCAGTTAAAAAGCTCGTAGTTG441300.18997689970442297No Hit
AGCTCCATTAATATAGATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG421080.1812723157206853No Hit
ACCATCATCAATCAAGAACGAAAGTGCCTGACTGACTTCCGACACAATTA420600.18106567871216928No Hit
ACATATTCTTTTGGGACAGGGTAAAGGCGTTGTACCGGAATATCTTCCAG393300.1693131988528202No Hit
AGCTCTGCGAGTTTGCTCCCATATTGTTGCAGTTAAAACGCTCGTAGTCT392150.16881813101991722No Hit
ACCATCATCAATCAAGAACGAAAGTGCCTGACTGACTCAGGCGTATTGGA389340.16760844353256296No Hit
AGCTCTAATAGTGTATATAAATGCTGTTGCGGTTAAAAAGCTCGTAGTTG385970.16615767953527336No Hit
AGCTCATGTAGTGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG383320.1650168710507578No Hit
GGGCACAGGGAAAAACATACAGGGATTGAAAACGAAGGTTTCTTCGGTAT376140.1619259257983722No Hit
AGCTCTTCTAGCGTATATCGAAGTTGATGCAGTTAAAACGCTCGTAGTCG321740.1385070648332224No Hit
ACCATCATCAATCAAGAACGAAAGTGCCTGACTGACTAATCCGTACAGCA312170.1343872394759341No Hit
ACCATCATCAATCAAGAACGAAAGTGCCTGACTGACTTGAGTCACTGGTA303640.1307151276370972No Hit
AGCTCTAATAGTGTATATAAATACTGTTGCGGTTAAAAAGCTCGTAGTTG288890.12436534456290677No Hit
AGCTCCAATAGCGCATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG288610.12424480630793908No Hit
AGCTCTAATAGTGTATATAAATTCTGTTGCGGTTAAAAAGCTCGTAGTTG277040.11926399341516733No Hit
AGCTCCAATAGCGTATATTAACGTTGTTGCAGTTAAAAAGCTCGTAGTTG273480.11773143560200679No Hit
AGCTCCAATAGCGTATATTAATGTTGTTGCAGTTAAAAAGCTCGTAGTTG263500.11343510779994437No Hit
ACCATCATCAATCAAGAACGAAAGTGCCTGACTGACTTTACTGTGCGATA260100.11197142898962252No Hit
AGCTCTAATAGTATATACGATCGTTGCTGCGGTTAAAAAGCTCGTAGTTG255130.10983187496394614No Hit
AACTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG254560.10958649351633337No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG245180.10554846197491599No Hit
AGCTCCAAGAGTGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG243580.10485967194652922No Hit
AGCTCTGCGAGTTTGCTCCCATATTGTTGCAGTTAAAACTCTCGTAGTCA240870.10369303383594916No Hit
AGCTCCAATAGTGTATATTAAAGTTGCTGTGATTAAAACGCTCGTAGTTG237140.10208729208227253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCCA10922300.0294.14921
GCTCCAA10448700.0293.44292
AATAGCG7579200.0293.129767
ATAGCGT7562550.0292.985238
TAGCGTA8440200.0292.805029
CTCCAAT8318800.0292.36693
CTGCGAG3188650.0290.832125
AGCTCTC2574000.0290.335451
GCTCTGC3303900.0289.160522
CCAAAAG1474850.0287.769265
CTCTGCG3216150.0287.2633
GCGAGTT3280750.0284.803227
CCAATAG8142250.0284.68335
TCCAATA8543050.0284.04334
TGCGAGT3295950.0283.43616
CAATAGC7895750.0281.715976
TCTGCGA3293050.0281.531224
CTAAGTG923550.0280.913577
AGCTCCG259950.0279.169071
CTCTAAG932250.0278.513525
Produced by FastQC (version 0.11.2)