FastQCFastQC Report
Wed 6 May 2015
000000000-AFBVR l01n03.333000000094c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFBVR l01n03.333000000094c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18239725
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAGACAA200828811.010516880051647No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGGGAGT8839174.846109247809383No Hit
AGATACCATGTTACGACTTTCTCATTCTTCAGATGATTTGGTTCACAAGA6369543.4921250183322394No Hit
GGATACCTTGTTACGACTTAGTCCCAATCAGTGGTTTTACCTTAGAGCCC5344912.9303676453455303No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAA5151502.8243298624293955No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAGACAG4655732.552522036379386No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAA3430691.8808891033170732No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAGATGATAAGGTTTAGACAA2922561.6023048593112013No Hit
GGATACCTTGTGACGACTTAACCTACCTTGTCTTACAAAGGTTCGACCCT2510451.3763639528556488No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC2201901.2072002182050443No Hit
GGATACCGTGTTACGACTTCTTCATCCTCTAGATGATAAGGCTCAGGGGA2025781.1106417448727983No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCGCTGCGATTAAATTG1725950.9462587840551324No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGACCAAGTTTGAGCAA1709430.937201629958785No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAG1452410.7962894177406732No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA1450370.7951709798256278No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACTTAA1230830.6748073230270741No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACGAA1131990.6206179095353686No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATATGGTTTACTTGA1065930.5844002582275775No Hit
GGCCACCTTGTTACGACTTTTGCCCGGTTCAAGCCAATGCGATCAAATTG1030340.564887902641076No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATTCA1029390.564367061455148No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGATGA994680.5453371692829799No Hit
GGATACCTTGTTACGACTTAACCCCGCTTACTAAGCCTAAGTTTGAACGT977890.5361319866390529No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC973200.5335606759422086No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGGTAG927290.5083903403148896No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGATAA906700.4971017929272508No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC888540.4871455024678278No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGAGCAAGTTTGATTTC883760.4845248489217902No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT832180.45624591379530116No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC762950.4182902976881504No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATAATAAGGTTTAGACAA755910.41443059037348423No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGAACAA755000.41393167934275327No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTTGACAA736980.4040521444265196No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAACGACCAAGTTTGACTAA699760.38364613501574174No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGTTCAAGTTTGGGCAT691950.3793642722135339No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAACGTAGTTCGATCAA605520.33197868937168734No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCCA588220.3224938972489991No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACCAA581410.31876028832671544No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGACAA545410.29902314864944507No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGTAAGGTTTGAACAA527140.2890065502632304No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGAGCAA510480.2798726406236936No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAA492770.2701630644102364No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGGACAA485510.26618274124198693No Hit
GGATACCTTGTTACGACTTAGCCCCCCTCACCTATCTCTGGTTCGACAGC485140.265979887306415No Hit
GAATACCTTGTTACGACTTTTACTTCCTCTAAATGATCCAGTTTGAGCAT482030.2642748177398508No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCACTAAA472600.25910478365216577No Hit
GGATACCTTGTGACGACTTATCCCACCTTGCTGAGCTTAGGTTCGACGCA433000.23739393000716844No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGATTTGCTTAA426040.23357808300289612No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGGGAGGGTTTAATGAA418040.22919205196350276No Hit
GAAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGCTTGAACTT400950.21982239315559857No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAACA400290.21946054559484862No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAACCGCTGCGATTAAAATG397630.2180021902742503No Hit
AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT394760.2164287016388679No Hit
GGATACCTTGTTACGACTTAACCCCGCTTACTAAGTCTAAGTTTGAACGC387650.21253061655260702No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAATCAA375580.2059131922219222No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC374510.20532656057040333No Hit
CAAGCAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGG348900.1912857787055452No Hit
GGAAACCTTGTTACGACTTCAGCTTCTTCTAAATAGATAGGTTTAAGAAA345750.189558778983784No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGAGCAAGTTTGATCCA344630.18894473463826894No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTAAAAAAA342090.18755216978326153No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGTTAAGGTTCACTAAA319020.17490395277341078No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC318290.17450372744106613No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTTGACAG313890.17209141036939976No Hit
AGCAACCTTGTTACGACTTTTGCCTCTTCTAGATAGTAAGTATCGACAAA311870.17098393753195293No Hit
GGAAACCTTGTTACGACTTCTTCTTCCTCTAGTCGTCCAAATTTACAAAA308430.16909794418501375No Hit
GGATACCTTGTTACGACTTAACCCCGCTTACTAAGCCTAAGTTTGAACGC304290.16682817312212767No Hit
AGATACCTTGTGACGACTTAACCTACCTTGTCTTGCAAAATTTCTTTTCA302080.16561653204749524No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAACGACCAAGTTTGATCGA301970.16555622412070356No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCATACCGATCAAACTG298440.16362088792457125No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGGAAAATATCATTATACTT279660.1533246800595952No Hit
GGAAACCTTGTTACGACTTTTACGTGGTCGAGACCATCAACTTTAGGCTA276170.1514112740186598No Hit
GGATACCTTGTTACGACTTAACCCCGCTTGCTAAGCCCAAGTTTGAACGC275960.15129614070387576No Hit
GGTTACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAA273820.150122877400838No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGAGCAA273580.1499912964696562No Hit
AGATACCATGTTACGACTTTCTCATTCTTCAGATGATTTGGTTTACAAGA260830.14300105950062297No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAACGACCAAGTTTGACCAA257400.14112054869248303No Hit
GGATACCTTGTTACGACTTAACCCTCCTTGCGAAATCGCGATTCGAGAAG250080.13710733029143807No Hit
AGATACCTTGTGACGACTTAACCTACCTTGTCGTGAAAAGGTTCTTCCCA247060.13545160357406705No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGGAGAA242850.13314345473958628No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGGACAA241240.13226076599290834No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAATTAA239850.13149869309981374No Hit
GGAAACCTTGTTACGCCTTATCCTTCCTCTAAGTGATATGGCTTACACAA238720.13087916621549942No Hit
AGATACCATGTTACGACTTTCTCATTCTTCAGATGATTTGGTTCTCAAGA236760.12980458861084804No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGT235120.1289054522477724No Hit
TGCTACCTTGTTACGACTTCACCCCAGTCATGAAACATACCGTGGTAATC234130.12836268090664743No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATAAGGTTTACTTAT233330.1279240778027081No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGCAAGGTTAGGATAA232790.12762802070754906No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCACGAAA231300.12681112242646203No Hit
AAGTACCTTGTTACGACTTTTGCCTCTTCTAGATTGGGAGTATTGCCAAA224580.12312685635337156No Hit
GGATACCTTGTTACGACTTAACCCCGCTTACTAAGCCTAAGTTTGAATGC223900.12275404371502312No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAAGCAA223660.12262246278384131No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAA220870.12109283445885285No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGATTCCACCGTGGAGGGT217810.11941517758628489No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAGTAATCAAGTTTGGACAA215070.11791296195529265No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC213200.11688772719983442No Hit
GGATACCTTGTTACGACTTAACCCCGCTTACTAAGCCTAAGTTTGAATGT213150.11686031450583823No Hit
GGAGACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACGAA210780.11556095281041792No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCGCTGACCTTACCGTGGCCGGC205340.11257845170363041No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGCAAAGGTTTACTTAA201960.11072535358948668No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAG199100.10915734749290353No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCCAATCGCAATCCTGCGATGCA197070.10804439211665746No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTAGAAAAA193950.10633384001129402No Hit
TCGTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTAAAGTG193260.10595554483414636No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGATTTGTTTAA192990.10580751628656682No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAAGCGAGTAGATTCACATCA190140.10424499272878292No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCACTAGA190140.10424499272878292No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGTCCAA190040.1041901673407905No Hit
GGATACCTTGTTACGACTTAACCCCGCTTGCAGAATCGAAGTTCAAACAC186590.10229869145505209No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGAGCAAGTTTGATTCA185920.10193136135550288No Hit
GGATACCTTGTTACGACTTAACCCCGCTTGCAGAATCGAAGTTCAAGCAC185790.10186008835111275No Hit
AGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAGACAA183680.10070327266447274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCT2346450.094.8319852
CTTGTTA15112600.094.77347
CCTTGTT15147350.094.761056
TTGTTAC15115550.094.711228
TGTTACG16155900.094.648079
CCTTGTG857900.094.6290366
ACCATGT799000.094.393625
AACCTTG11325700.094.31014
AAACCTT11181700.094.291013
GAAACCT11087450.094.275992
CGTGTTA271250.094.264527
CCATGTT800550.094.246466
AGATACC1303100.094.234531
GTTACCT957850.094.152012
CTTGTGA844400.093.886197
TTGTGAC844400.093.863698
CATGTTA805400.093.684827
ACCGTGT271750.093.3744355
GGAAACC11123550.093.055351
GATACCA809500.092.6411362
Produced by FastQC (version 0.11.2)