##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AFBVR l01n02.332000000094c8.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18239725
Sequences flagged as poor quality	0
Sequence length	12
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.092160600009045	33.0	32.0	34.0	18.0	35.0
2	31.354475190826616	33.0	32.0	34.0	18.0	35.0
3	31.533296527222863	33.0	32.0	34.0	29.0	35.0
4	31.686848239214132	33.0	32.0	34.0	29.0	35.0
5	31.32199739853534	33.0	32.0	34.0	18.0	35.0
6	33.64420390110048	37.0	33.0	37.0	20.0	37.0
7	33.36251730768967	35.0	33.0	37.0	29.0	37.0
8	32.81686028709314	34.0	33.0	37.0	19.0	37.0
9	33.36546291131034	37.0	33.0	37.0	18.0	37.0
10	33.14098195011164	35.0	33.0	37.0	19.0	37.0
11	33.023375571726	35.0	33.0	37.0	19.0	37.0
12	33.39458215515859	37.0	33.0	37.0	18.0	37.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.17036480638731177
1101	2	-0.13654239163035697
1101	3	0.030207867969906488
1101	4	-0.052251156008463084
1101	5	0.07656267486575175
1101	6	0.07854597771676453
1101	7	0.06707947169975625
1101	8	0.3359515266748403
1101	9	0.3041138141549169
1101	10	0.11481126286768273
1101	11	0.16595893655949112
1101	12	0.1064129891724761
1102	1	0.4449823149566896
1102	2	0.38637119036836864
1102	3	0.29841732003043475
1102	4	0.29672144281464696
1102	5	0.48649584149570657
1102	6	0.3467019040970527
1102	7	0.38819703675519435
1102	8	0.5463556286964462
1102	9	0.571191340597828
1102	10	0.40896769705813796
1102	11	0.35159181044181764
1102	12	0.33771419379448275
1103	1	0.4064622229119408
1103	2	0.40245724451863296
1103	3	0.3575992714062757
1103	4	0.2627491677715774
1103	5	0.3958498942882258
1103	6	0.17001703402541324
1103	7	0.31606489233263346
1103	8	0.4670342371295817
1103	9	0.46199235034029584
1103	10	0.27913144344195473
1103	11	0.30972094754365287
1103	12	0.06616630847633331
2112	1	0.25470691848279614
2112	2	0.16303977083378385
2112	3	0.17796489227072954
2112	4	0.16673549895624973
2112	5	0.28614345232906047
2112	6	0.16111746189427123
2112	7	0.14375925022978464
2112	8	0.3997881334542157
2112	9	0.3838802717077101
2112	10	0.14294136881508734
2112	11	0.1851545075265406
2112	12	-0.08886734427520082
1113	1	0.29893433410310166
1113	2	0.08272435426498603
1113	3	0.05925798765702339
1113	4	0.06940442526278545
1113	5	0.197400514192708
1113	6	0.07281866900384415
1113	7	-0.06752338723883611
1113	8	0.23411309987025675
1113	9	0.34032742958597595
1113	10	0.025716715051757433
1113	11	0.10949480194236116
1113	12	4.1527614391867473E-4
2113	1	0.20627298523796256
2113	2	0.18351308817283396
2113	3	0.13142249875622625
2113	4	0.10064502754201143
2113	5	0.26524805269028917
2113	6	0.11665324776629404
2113	7	0.09998743556548817
2113	8	0.3012385737564429
2113	9	0.35923442849179565
2113	10	0.17141005649842356
2113	11	0.16609460638603935
2113	12	0.07728502439584162
1112	1	0.21652764044881323
1112	2	0.2169352981024879
1112	3	0.2134484515218702
1112	4	0.13680426000771106
1112	5	0.3908383919393934
1112	6	0.10200865531545134
1112	7	0.2113904018091759
1112	8	0.37939313264948993
1112	9	0.3815779649637179
1112	10	0.16906248603668672
1112	11	0.06254272114512105
1112	12	-0.0233629516707623
2114	1	0.415014780942883
2114	2	0.21356813553992993
2114	3	0.0867824895460707
2114	4	0.13233165127735447
2114	5	0.3226119869839472
2114	6	0.0023797708097674786
2114	7	0.17935111748189314
2114	8	0.4534704059810366
2114	9	0.44416839483384507
2114	10	0.24916887702880786
2114	11	0.24052890583610065
2114	12	0.10217474629259726
1114	1	0.2058054533088267
1114	2	0.10215329390791084
1114	3	0.21958777874437985
1114	4	0.18519206980960234
1114	5	0.16501898722436792
1114	6	0.10139024418404574
1114	7	0.03408991319275856
1114	8	0.35601288948429755
1114	9	0.3816320425344628
1114	10	0.17292350001702772
1114	11	0.08807700695535203
1114	12	0.06674406282547807
1109	1	0.28335444244803654
1109	2	0.17357494390365247
1109	3	0.08610214533479521
1109	4	0.09415147347186803
1109	5	0.3514634349100909
1109	6	0.11575950818781422
1109	7	0.06691623180747541
1109	8	0.30049309131833724
1109	9	0.2983607820472187
1109	10	0.19685952948819363
1109	11	0.18447617659218452
1109	12	0.19250795917064778
1108	1	0.39182184646175244
1108	2	0.28360300356170853
1108	3	0.3226309589697358
1108	4	0.20328074859564538
1108	5	0.30401144121576706
1108	6	0.2271061581991347
1108	7	0.27026396831718813
1108	8	0.3349615252675804
1108	9	0.4134889238607755
1108	10	0.20624183045042344
1108	11	0.10908007308420764
1108	12	0.1624346685620921
1111	1	0.14146663974036144
1111	2	0.06413407446173025
1111	3	0.07020438517589156
1111	4	-0.21646716369846786
1111	5	0.20279841958378952
1111	6	-0.08588382926559035
1111	7	0.08195914225900935
1111	8	0.3308013064761326
1111	9	0.3510223285656693
1111	10	0.15212641516067293
1111	11	0.04323646122495006
1111	12	-0.012302260822728783
1110	1	0.11431478065257039
1110	2	0.15805275815446507
1110	3	0.18129705118916561
1110	4	0.11358044972614678
1110	5	0.20859474618857377
1110	6	0.006507722402609772
1110	7	-0.5847554934734163
1110	8	0.0023499422097899014
1110	9	0.1996843104918824
1110	10	-0.1023019624958863
1110	11	0.017198723038177377
1110	12	0.03386525270963858
1105	1	-0.06469617649078785
1105	2	-0.026102775903453335
1105	3	0.07562028806879084
1105	4	0.02818111243840704
1105	5	0.04420033695894787
1105	6	-0.23606038138193242
1105	7	-0.06749287537063253
1105	8	0.18336537104259065
1105	9	0.2481996566930107
1105	10	-0.25804421253118903
1105	11	-0.03267245487887749
1105	12	0.023125660783733792
1104	1	-1.6629411147056778
1104	2	-1.1802558909300167
1104	3	-0.5767748376223913
1104	4	-0.4267545379189954
1104	5	-4.583138457829168
1104	6	-1.1567034198860355
1104	7	-0.7063640380645779
1104	8	-0.08739868099986836
1104	9	0.04016259587012172
1104	10	-0.17674019372774552
1104	11	-0.06028796736373465
1104	12	0.16618720810463117
1107	1	-0.1052583692158855
1107	2	-0.34499107647911487
1107	3	-0.25792707405554793
1107	4	-0.1741978674293918
1107	5	0.023202896886164837
1107	6	-0.45208048296101566
1107	7	-0.12062003333685567
1107	8	0.11301868990546637
1107	9	-6.148150913208678
1107	10	-1.149270222324791
1107	11	-0.4524679369638136
1107	12	-0.008576882282142151
1106	1	-0.18838127327560628
1106	2	-0.23680881575635127
1106	3	-1.0385257250380917
1106	4	-0.45336577806781975
1106	5	-0.046902148348198835
1106	6	0.13376718519920416
1106	7	-0.11047589914174694
1106	8	0.2176488385848927
1106	9	0.30710252861276643
1106	10	-0.3033717318471787
1106	11	-0.09160374661600201
1106	12	0.12712712937208437
2103	1	0.20678402135411034
2103	2	-0.0030847973676486617
2103	3	0.12465594202499375
2103	4	0.054469991693402164
2103	5	0.18073085282645351
2103	6	0.2460732668891481
2103	7	-0.1279561506781448
2103	8	0.25671151223662037
2103	9	0.2438020898624771
2103	10	0.10579748315957005
2103	11	0.09856486625015037
2103	12	0.1995240527069626
2102	1	-0.11720447988448512
2102	2	-0.3950128684270524
2102	3	-0.03492313024576532
2102	4	-0.04633875495451534
2102	5	0.18572678871361603
2102	6	0.13883971462404787
2102	7	-0.01896595699189163
2102	8	0.1564785904441166
2102	9	0.05334264668481836
2102	10	0.07593979208817103
2102	11	0.12741286720639522
2102	12	-0.22610603000027396
2101	1	-0.27290115876869336
2101	2	0.049093819317409526
2101	3	-0.15578766683354672
2101	4	-0.06077481330898493
2101	5	0.2172241310201315
2101	6	-0.004975176577701745
2101	7	0.1481977106000727
2101	8	0.14430528291836708
2101	9	0.23593633950191162
2101	10	0.13633814029916635
2101	11	-0.7463039804411551
2101	12	-0.28038966354515793
2107	1	0.020489486473522334
2107	2	0.24744488225345762
2107	3	0.1195634878225178
2107	4	0.15215246215131728
2107	5	0.22143236077217665
2107	6	0.24720322856262555
2107	7	0.26594390208721563
2107	8	0.4143644699184037
2107	9	0.44606607861591385
2107	10	0.21983608912799468
2107	11	-0.04154325796172742
2107	12	0.16071368271266095
2106	1	0.06375221193638225
2106	2	0.009453143457665902
2106	3	-0.027020994285425814
2106	4	-0.041289850683877916
2106	5	0.1552518731784467
2106	6	0.0010760516493917294
2106	7	0.09871248173639913
2106	8	0.2991678675804792
2106	9	0.17137055134679002
2106	10	-0.01879837790094996
2106	11	0.12526971960837585
2106	12	-0.27035181926859764
2105	1	0.2341462352124104
2105	2	0.13693244862740173
2105	3	0.16468859605590325
2105	4	0.08333621456733553
2105	5	0.22210322103521918
2105	6	0.2356883326150907
2105	7	0.2448146142731744
2105	8	-6.413993144664435
2105	9	-1.0132190419682203
2105	10	-0.5304547615845294
2105	11	-0.24040383736987536
2105	12	-0.06807102777065666
2104	1	-0.018992449271379996
2104	2	0.07673661546267496
2104	3	0.044754220937516465
2104	4	0.03075064304495001
2104	5	0.018939853580793198
2104	6	-0.008703689119919034
2104	7	0.05335735167250988
2104	8	0.24521587113638077
2104	9	0.13880465133244257
2104	10	0.1320776803049597
2104	11	-0.05990106591683997
2104	12	-0.05244364046413352
2111	1	-0.37957080326210146
2111	2	-0.22518541365023026
2111	3	-0.002508237892584475
2111	4	-0.11996947021942361
2111	5	-0.04124899347176836
2111	6	-0.02509358341350776
2111	7	-0.10346005427303595
2111	8	0.16275933120989095
2111	9	0.17289412950870542
2111	10	-0.05470044098187543
2111	11	-0.15301821781100955
2111	12	-0.3177628114199891
2110	1	-0.2317034274867673
2110	2	-0.10312517876733551
2110	3	-0.1327651531590739
2110	4	-0.09062092719689474
2110	5	-0.0034456385056813588
2110	6	-0.004581577112517721
2110	7	-0.47550960979840795
2110	8	-0.16745778744820683
2110	9	0.011971950125179376
2110	10	-0.17320746777176765
2110	11	-0.20569876847481794
2110	12	-0.43313792046240707
2109	1	-0.45675366727838806
2109	2	-0.2147298728304392
2109	3	-0.11608501980665764
2109	4	-0.1796339059594878
2109	5	-0.09986479089952027
2109	6	-0.22408812432582437
2109	7	-0.1426143583164574
2109	8	0.06299990117565102
2109	9	0.11995331588874336
2109	10	-0.10424860123848134
2109	11	-0.16185453770738434
2109	12	-0.15729030733338334
2108	1	-0.2360685886452103
2108	2	-0.08394898316723243
2108	3	-0.42188779454328085
2108	4	-0.2488224136848025
2108	5	-0.1472501238252022
2108	6	-0.3054838690980475
2108	7	-0.14434706513558382
2108	8	-0.029149606008786577
2108	9	0.08108903895807629
2108	10	-0.08821239449033413
2108	11	-0.13864735983577248
2108	12	0.11626444409178305
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	23.0
15	2254.0
16	63707.0
17	151978.0
18	203613.0
19	269318.0
20	249567.0
21	271468.0
22	274581.0
23	255391.0
24	249809.0
25	241948.0
26	238293.0
27	244692.0
28	271241.0
29	302862.0
30	390878.0
31	521162.0
32	971863.0
33	2170699.0
34	4896179.0
35	5273578.0
36	724621.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.311394497449935	21.395854378286952	41.263012463181326	19.029738661081787
2	31.963149878975685	18.2969445314493	18.773278519635415	30.966627069939605
3	5.135115798072613	42.04828197793552	13.615868660300526	39.20073356369134
4	45.9231236174407	17.80259394275922	25.891307346536603	10.382975093263473
5	19.93172703709771	33.92605611795442	21.224438483827935	24.91777836111994
6	25.006994348873135	32.79749009373771	13.135993004280492	29.059522553108668
7	17.66847362007925	28.02060338080755	15.02306092882431	39.28786207028889
8	5.56447016413187	24.41816005549207	19.72657042398229	50.29079935639377
9	12.35040001973714	35.76336814288592	31.229675886012537	20.656555951364396
10	32.90244233397159	25.181733825482567	17.40584904651797	24.50997479402787
11	19.733866601607204	9.96775444805226	24.568369314778597	45.73000963556194
12	9.272179268053659	37.96328617893088	31.928107468725543	20.836427084289923
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	694.0
1	694.0
2	694.0
3	694.0
4	2366.5
5	4039.0
6	4039.0
7	4039.0
8	4039.0
9	4039.0
10	4039.0
11	4039.0
12	4039.0
13	10657.5
14	17276.0
15	17276.0
16	17276.0
17	17276.0
18	17276.0
19	17276.0
20	17276.0
21	38607.0
22	59938.0
23	59938.0
24	59938.0
25	59938.0
26	59938.0
27	59938.0
28	59938.0
29	148141.0
30	236344.0
31	236344.0
32	236344.0
33	236344.0
34	236344.0
35	236344.0
36	236344.0
37	236344.0
38	2474140.5
39	4711937.0
40	4711937.0
41	4711937.0
42	4711937.0
43	4711937.0
44	4711937.0
45	4711937.0
46	6531045.0
47	8350153.0
48	8350153.0
49	8350153.0
50	8350153.0
51	8350153.0
52	8350153.0
53	8350153.0
54	6469876.0
55	4589599.0
56	4589599.0
57	4589599.0
58	4589599.0
59	4589599.0
60	4589599.0
61	4589599.0
62	4589599.0
63	2398411.5
64	207224.0
65	207224.0
66	207224.0
67	207224.0
68	207224.0
69	207224.0
70	207224.0
71	128176.0
72	49128.0
73	49128.0
74	49128.0
75	49128.0
76	49128.0
77	49128.0
78	49128.0
79	29908.0
80	10688.0
81	10688.0
82	10688.0
83	10688.0
84	10688.0
85	10688.0
86	10688.0
87	10688.0
88	6329.5
89	1971.0
90	1971.0
91	1971.0
92	1971.0
93	1971.0
94	1971.0
95	1971.0
96	1352.5
97	734.0
98	734.0
99	734.0
100	734.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.096507759848353E-5
3	0.0
4	5.482538799241765E-6
5	5.482538799241765E-6
6	0.0
7	0.0
8	5.482538799241765E-6
9	0.0
10	0.0
11	0.0
12	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
12	1.8239725E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	2.3498711255848828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.01191409340333	1.668688465006027
2	11.225251837751673	0.5275579034190259
3	4.705847365113602	0.33174404534070456
4	2.7675720202087413	0.2601375031306057
5	1.7699733777095037	0.2079604666666754
6	1.250658477225291	0.17633317461598022
7	0.930607644324259	0.15307656228522998
8	0.634043873574369	0.11919371126931212
9	0.4838378284856836	0.10232608883617789
>10	4.258302716025629	1.9919743057364145
>50	0.48228471577161597	0.7869647119910591
>100	0.35252911923772295	1.7192460295378476
>500	0.05876295931456861	0.9780502352216576
>1k	0.05914096039426796	2.8148283065876196
>5k	0.003709204583902785	0.5789704464586191
>10k+	0.005563806875854088	87.58294804389708
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGAGAACCTCCA	2365886	12.971061789582903	No Hit
TCCGACACAATT	1487514	8.155353219415314	No Hit
CCAGTGTATGCA	1050025	5.7568028026738345	No Hit
TAATGACCACGC	995927	5.460208418712453	No Hit
ATTTCGACATGC	946005	5.186509116776706	No Hit
CTAACCTCCGCT	903625	4.95415912246484	No Hit
ACCGTGCATAGG	830443	4.552935968058729	No Hit
AGCAGTCGCGAT	830191	4.55155436828132	No Hit
AGTTCAGACGCT	783467	4.2953882254255475	No Hit
GCGGATGTGACT	767801	4.209498772596627	No Hit
TGCCTACAAGCA	738944	4.051289150466906	No Hit
CACGCCATAATG	669860	3.6725334400600884	No Hit
GTATGCGCTGTA	655430	3.5934204051870298	No Hit
GACTTGGTATTC	653737	3.5841384669999137	No Hit
TACACGATCTAC	605388	3.3190631985953734	No Hit
GGAGACAAGGGA	471518	2.5851157295408784	No Hit
GCACACACGTTA	438620	2.404751168123423	No Hit
TTCAGACTACCA	424873	2.3293827072502467	No Hit
CCTCGTTCGACT	345491	1.8941678122888366	No Hit
TGAAAACCTCCA	46610	0.25554113343265866	No Hit
TGAGAACCTCAA	33195	0.18199287544083037	No Hit
>>END_MODULE
>>Adapter Content	warn
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTGC	345	0.0	6.0000005	1
AGGGTAT	450	0.0	6.0000005	3
AGAACCG	305	0.0	6.0000005	3
CGTACGC	205	0.0	6.0000005	5
CCGACCA	205	0.0	6.0000005	2
GTACGCA	395	0.0	6.0000005	6
GCATAAG	115	6.8277586E-8	6.0	6
AGCCGTC	120	2.5696863E-8	6.0	1
CAGAAAC	115	6.8277586E-8	6.0	2
GAGTGCA	55	0.009160909	6.0	2
GACAGGG	70	4.6975462E-4	6.0	4
GTCGCTA	190	0.0	6.0	5
GACAGAA	135	1.375156E-9	6.0	4
GTCGCGT	165	3.6379788E-12	6.0	5
ACAGTGT	190	0.0	6.0	1
AGTCCGA	80	6.539671E-5	6.0	4
CCGGGCA	310	0.0	6.0	2
GATAACC	655	0.0	6.0	2
GTCGAGA	355	0.0	6.0	5
AGTCAGA	125	9.677024E-9	6.0	1
>>END_MODULE