FastQCFastQC Report
Wed 6 May 2015
000000000-AFBVR l01n02.332000000094c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFBVR l01n02.332000000094c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18239725
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGAACCTCCA236588612.971061789582903No Hit
TCCGACACAATT14875148.155353219415314No Hit
CCAGTGTATGCA10500255.7568028026738345No Hit
TAATGACCACGC9959275.460208418712453No Hit
ATTTCGACATGC9460055.186509116776706No Hit
CTAACCTCCGCT9036254.95415912246484No Hit
ACCGTGCATAGG8304434.552935968058729No Hit
AGCAGTCGCGAT8301914.55155436828132No Hit
AGTTCAGACGCT7834674.2953882254255475No Hit
GCGGATGTGACT7678014.209498772596627No Hit
TGCCTACAAGCA7389444.051289150466906No Hit
CACGCCATAATG6698603.6725334400600884No Hit
GTATGCGCTGTA6554303.5934204051870298No Hit
GACTTGGTATTC6537373.5841384669999137No Hit
TACACGATCTAC6053883.3190631985953734No Hit
GGAGACAAGGGA4715182.5851157295408784No Hit
GCACACACGTTA4386202.404751168123423No Hit
TTCAGACTACCA4248732.3293827072502467No Hit
CCTCGTTCGACT3454911.8941678122888366No Hit
TGAAAACCTCCA466100.25554113343265866No Hit
TGAGAACCTCAA331950.18199287544083037No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGC3450.06.00000051
AGGGTAT4500.06.00000053
AGAACCG3050.06.00000053
CGTACGC2050.06.00000055
CCGACCA2050.06.00000052
GTACGCA3950.06.00000056
GCATAAG1156.8277586E-86.06
AGCCGTC1202.5696863E-86.01
CAGAAAC1156.8277586E-86.02
GAGTGCA550.0091609096.02
GACAGGG704.6975462E-46.04
GTCGCTA1900.06.05
GACAGAA1351.375156E-96.04
GTCGCGT1653.6379788E-126.05
ACAGTGT1900.06.01
AGTCCGA806.539671E-56.04
CCGGGCA3100.06.02
GATAACC6550.06.02
GTCGAGA3550.06.05
AGTCAGA1259.677024E-96.01
Produced by FastQC (version 0.11.2)