FastQCFastQC Report
Wed 6 May 2015
000000000-AFBVR l01n01.331000000094cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFBVR l01n01.331000000094cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18239725
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGAGCTCCGGA8570124.698601541415783No Hit
GCCTCAATCGACTGGATGATCCGGTGAATGATTCGGACCGGAAATATGTT7254923.9775380385395063No Hit
AAGTCATGAAAGGTGGGAGTACCCGAAGCGCCCTTAGCTAATTAAGCGGG5536253.0352705427302222No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGCGATGAGTTT4059412.2255872827030014No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTC3943372.1619679024766No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTTT3658962.0060390164873647No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTTC3598591.972940929756342No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGGCTTTGGGGA3316361.818207237225342No Hit
AAACCAACCGAGTTTTGCATTGGTGAGGCCCGTAAGGGTCGAACCTTTGT2544611.3950923053938586No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG2241001.2286369449100796No Hit
GCTGCCCGGAACTGAGCCGTTTCGAGAAGACTGGGGATTGACGCTTTACG1955981.0723736240540909No Hit
GCTTCAACCGATTGGATGATCCGGCAAAGTGGACGGACAGGGTACCAGTT1687440.9251455271392524No Hit
GCACCTACCGATTGGATGGTCCGGTGAAGCCTCGGGATTGTGACCGGTGC1525950.8366080080702972No Hit
GCTACTACCGATTGAATGGCTTAGTGAGACCTCCGGATCGGCTTTGGGGA1438910.7888879903616968No Hit
GCTGCCCGGAACTGGGTTAATTCGAGAAGATTGAGGATTGTTTTTATGTG1194410.6548399167202356No Hit
GCTACTACCGATTGGATGGCTTAGTGAGATTTTCGGATTGATTGGCGATT1158790.6353111135173365No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTTTCCGGATTGGCTTTGAGGA999430.5479413752126197No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGACTTTGGCA989240.5423546681761924No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGCGATGGGGTT924550.5068881246838973No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGAGATTGTGACCAGTTT863280.47329660946094304No Hit
GCTACTACCGATTGAATGGATTAGTGAGCTTTCAGGATTGTTGCACCATG792750.4346282633098909No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGGGAATGTGACCAGTTC791490.43393746342118644No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC753720.4132299143764503No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGAGATTATGACCATTAC737390.4042769285172885No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCTCAGGGAG733980.40240738278674704No Hit
GCTACTACCGATTGAACGGCTTAGTGAGGCTTTCGGATTGGCCCGGGGAG712320.3905322037475894No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG698810.3831252938298138No Hit
GCTACTACCGATCGAATGGCTCAGTGAGGCCTTGGGACTGGCTTAAGGAG681060.37339378746115964No Hit
GCTACTACCGATTGAATGTTTTAGTGAGGTCTTCGGACTGGCCGGCGCAG677840.3716284099678038No Hit
GCTACTACCGATTGGATGGCTTAGTGAGATTTTCGGATTAGCTAATGATT602820.3304984038958921No Hit
AAACCATGCGAGTTGGGTTTGAGTGAGGATGTAGTTTTTGCTACGTTCAA600860.3294238262912407No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTGC589550.32322307490929825No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGATCTGAGCA542270.2973016314664832No Hit
GCTACTACCGATTGAATGTTTTAGTGAGATCTTCGGCTTGGTTTTCCGAG533950.29274015918551405No Hit
GCTACTACCGATTGAATGGCTTAGTGAGACTTTCGGATTGATTTGCGATT515910.28284965919168187No Hit
AAGTCACCCAAGCTTGTTTTTGGTGAGGCTGCGCTTCTTGGCGCTGTCGA513890.28174218635423504No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCGGTGC489730.26849637261526693No Hit
GCACCTACCAATTGAATGGTCAGGTGAAGCCTCGGGATTGCAATGAGTTT482160.2643460907442409No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTGGGACTGGCTCAGAGAG452070.2478491314973225No Hit
GCTGCCCGGGACTGAGCCGTTTCGAGAAGAACAGGGACCGTCGTTGAAGG445220.24409359241984188No Hit
GCTCCTACCGATTGAGTGATCCGGTGAACTTTTTGGACCTGTCGCCTCGC444370.2436275766219063No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGCGACCAGTTT442460.24258041171125114No Hit
AATCCAACCGAGCAGGGTTTAGGTGAGCCGCATTGAGTAAATGCGTCGAA441320.24195540228813756No Hit
GCTGCCCGGGACTGAACCGTTTCGAGAAGACTGGGGATTGCTGACTTAGG427140.23418116227081273No Hit
GCTACTACCGATTGGATGACTTAGTGAGGTCTTAGGACCGAGGTAGAGGT406670.22295840534876485No Hit
GCTACTACCGATTGAATGGCTTAGTGAGATTTTCGGATTGATTGGCGATT398070.21824342198141694No Hit
AAACCATGCGAGTTGGGCCTGAGTGAGGATGTAGTTTTTGCTGCGTTCAA382890.20992092808416793No Hit
ACACCATGGGAGTGGGTTGTACCAGAAGTAGATAGCTTAACCTTCGGGAG382600.20976193445898994No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTCCAGGGATTGTTGTGCTTGG376780.2065710968778312No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTTCAGATTGGCTTCGGAGG372070.20398882110338837No Hit
GCACCTACCGATTGGATGGTCCGGTGAAGCCTCGGGATTGTGACTGGTGC361000.1979196506526277No Hit
GCTGCCCGGAACTGAGCCATTTCGAGAAAGCTGGGGACTGACGGCTTGTG357530.19601720968929082No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGACTCGAGACTGTGGTCTGGCT352660.1933472132940601No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTGC348820.19124191839515126No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTGGGACTGGCCCAGAGAG345110.18920789650063255No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGCTGTGAGTTT336830.18466835437486037No Hit
GCTTCAACCGATTGGATGATCCGGCAAAGTGGATGGACAAGGTGCTTGCC325870.1786594918508914No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTGGGATTGGCCAGGGGAG323110.17714631114230067No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCTGGTTT321340.17617590177483486No Hit
GCTACTACCGATTGAATGGTTTAGCGAGATTCTCGGATTGGGCCTCGATC320610.1757756764424902No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGCGAATATGAC315210.17281510549089968No Hit
ACACCATGGGAGTGGGTTGTACCAGAAGTAGATAGCTTAACCTTCGGGGG313950.17212430560219522No Hit
GCTCCTACCGATTGAATGTTCCGGTGAATAATTCGGACTGCGGCTATGGG311330.17068788043679387No Hit
GCTACTACCGATTGAACGGCTTAGTGAGGTCTTCGGATTGGCTTCGGGGA310290.17011769640167274No Hit
GCAGCTACTGATTGAATATATCGGTGAAATATTGGGACTGTGATTACTTA308150.168944433098635No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGGGAATGTGACCAGCTC301500.1652985447971392No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAGTAGCTACC295920.1622392881471623No Hit
GCCTCAATCGACTGGATGATCCGGTGAATGATTCGGACTGGAGATATGTT290340.1591800314971854No Hit
GTTGCCTGTGATGGGTAGTGAGTAAAGCCATTTGGAGGATTTGTCTTCGG287520.1576339555557992No Hit
GCTACGAGGGAGTGGACCATTTACTGAAGTCTTTGGACTGGAGTGTTGTT285910.1567512668091213No Hit
GCTACTACCGATTGAATGATTCAGTGAGGCTTTCGGACTGGCCCAGGAAG284390.15591792091163653No Hit
GCTACTACCGATTGAATGGCTTAGTGAGATCTCCGGATTGGCTTTGGGAA276070.15135644863066738No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTTCAGGGATTGTTGCGTTTGG262400.1438618180921039No Hit
ACACCATGGGAGTTGATTTCACCCGAAGCGGGGAAGCTAAACTGGCTACC259720.1423924976939071No Hit
ACACCATGGGAGTCGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC257190.14100541537769895No Hit
AAACCAACCGAGTTGTGTAGGGGTGAAGCCCTTTTTGGGAAGAACCTTTT256530.14064356781694898No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTT256350.14054488211856264No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGTCTTGAGATTCTGATTATTGC255040.13982666953586195No Hit
GCACCTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGAGCTCCGGA254390.13947030451391126No Hit
GCACCTACCGATTGAATGGTCCGGTGAAATTTTCGGACTGTTGCAATGTC253940.13922359026794537No Hit
ACACCATGGGAGTGGAGGATACCAGAAGTAGTTAGGCTAACCGTAAGGGG245660.1346840481421732No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAG239500.13130680424184027No Hit
GCTCCTACCGATTGGGTGATCCGGTGAATAATTCGGACTGCTGTTACTTT238990.13102719476307895No Hit
AAACCATGCGAGTTGGGTCTGAGTGAGGATGTGGTCTTTGCCACGTTCAA237430.13017191871039724No Hit
ACACCATGGGAGTGGGTTGTACCAGAAGTAGATAGCTTAACCTTTTGGAG228930.12551176073104173No Hit
AAACCAACCGAGTTCTGTAGGGTTGAAGTTCAAATGAAAAGAAATTTTGC227740.12485933861393196No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCATTCGGACTGGCTCGGGGAG219880.12055006311772792No Hit
GCTCCTACCGATTGAATGACCCGGTGAGGCTTCCGGACTGCCTGCGTTGC217650.11932745696549701No Hit
GCTTCTACCGATTGGTCGTCCCGGTGAACAATCGGGAGGCCGTGCTCATG215720.11826932697724336No Hit
GCTACTACCGATTGAATGGTTTAGTGAGATCTCTGGAGCGACGCCTGAAC215510.11815419366245927No Hit
GCACCTACCGATTGAATGGTCCGGTGAATTCTTCGGACCGTAACATTGCT213820.11722764460538743No Hit
GCACCTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGCGACCAGTTC213050.1168054891178458No Hit
GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTGTCAAAGCT211600.11601052099195575No Hit
GCTCCTACCGATTGGGTGTGCTGGTGAAGTGTTCGGATTGGCAGCTTAGG209390.11479887991732332No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCATGTTG208790.1144699275893688No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGATTTTGGCA208310.11420676572700521No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCACGCTG207870.11396553401983855No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATCGGCTTCGGGGA206980.11347758806670605No Hit
AAACCATGCGAGTTGGGTTTGAGTGAGGATGTAGTTTTTGCTGCGTTCAA205890.1128799913375887No Hit
AAACCATGCGAGTGGGGTCGTAGTGAAGGCACGCTTCTTGGCGTGCTTGA205330.11257296916483116No Hit
AAACCATGCGAGTGGGGTCGTAGTGAAGGCACGCTTCTTGGCGTGTTTGA204760.11226046445327437No Hit
ACACCACGAAAGTCGGTTGTTCCAGAAGTAGTTGAGCTAACCTTCGGGAG203810.11173962326734642No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACTGTATGTG194070.10639963047688493No Hit
GCTCCTACCGATTTCGAGTGATCCGGTGAACCTTTTGGACCGCGGAGGGT193230.10593909721774862No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCGTTCGGACTGGCTCGGGGAG192340.10545115126461611No Hit
TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTCCTCG191680.10508930370386614No Hit
ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG190130.10423951018998366No Hit
ACACCATGGGAGTGGGGGATACCAGAAGTAGGTAGGGTAACCGCAAGGAG184920.10138310747557873No Hit
GTTGCCTGTGATGGATTGTGAGTAGAGCCATCTGGAGGTGGTTGCAGGGG184500.10115284084601056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTACC5039000.094.9745563
ACTACCG5040000.094.970794
CTACTAC5033100.094.951872
CGATTGA9220650.094.8851249
CTACCGA11024450.094.86865
CCTACCG5729300.094.85164
CACCTAC4216400.094.841162
CCGATTG10686450.094.821778
TACCGAT11088850.094.820956
TCCTACC1624250.094.818693
GCTACTA5031900.094.811211
ACCGATT11047500.094.787187
ACCTACC4210900.094.784553
GCACCTA4208800.094.7584841
CAATCGA835200.094.681525
AATCGAC808350.094.676816
CCGAGTT747400.094.650468
ACCATGG1292050.094.639733
TCAATCG829450.094.604864
CTCCTAC1643000.094.601042
Produced by FastQC (version 0.11.2)