##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AF4P8 l01n03.33300000008af9.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15963168
Sequences flagged as poor quality	0
Sequence length	251
%GC	55
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32898438455324	32.0	32.0	33.0	29.0	33.0
2	32.0517448666831	33.0	32.0	33.0	32.0	33.0
3	31.1952364969159	33.0	32.0	33.0	29.0	33.0
4	30.778045874102183	32.0	32.0	33.0	29.0	33.0
5	30.861315686209654	32.0	32.0	33.0	29.0	33.0
6	35.10388201139022	37.0	37.0	37.0	33.0	37.0
7	35.066718460896986	37.0	37.0	37.0	33.0	37.0
8	33.34866431274794	37.0	32.0	37.0	29.0	37.0
9	34.10493343175991	35.0	33.0	37.0	31.0	37.0
10-14	35.66790153433203	37.4	35.6	37.4	33.4	37.4
15-19	34.99473457900085	37.2	35.4	38.0	27.0	38.0
20-24	34.13613500778793	36.8	34.0	37.8	23.0	38.0
25-29	35.428366938191715	37.4	35.8	38.4	29.2	38.6
30-34	34.56164112286483	37.2	34.4	37.6	26.6	38.6
35-39	35.19875687582815	37.0	36.2	38.2	31.0	38.4
40-44	35.392975028515636	38.0	36.2	39.0	22.0	39.0
45-49	35.982012956325455	38.0	36.4	39.0	27.4	39.0
50-54	35.15213906161985	37.6	36.0	39.0	23.0	39.0
55-59	35.66019598365437	37.4	36.0	38.0	32.2	39.0
60-64	36.04240997776883	38.0	36.2	38.4	33.4	38.4
65-69	33.1530120712881	36.0	30.6	37.8	21.8	38.4
70-74	34.396337544026345	37.0	33.8	38.0	25.8	38.4
75-79	35.52874364286588	38.4	36.0	38.8	26.2	39.0
80-84	35.45817453026868	38.0	35.8	38.8	26.8	39.0
85-89	35.9528373315372	38.2	36.6	38.6	31.6	38.6
90-94	34.99551270775324	37.4	35.8	38.4	24.8	38.6
95-99	36.19834014150575	38.2	36.6	38.6	32.6	39.0
100-104	34.8763048287157	37.0	35.0	38.0	28.0	38.8
105-109	32.302442936138995	36.2	29.4	38.0	15.2	38.8
110-114	34.32047726366095	36.8	32.0	38.4	26.8	38.8
115-119	33.94999582789582	37.2	33.4	38.8	16.4	39.0
120-124	35.934075203618725	38.0	36.4	39.0	30.4	39.0
125-129	34.17255324256438	37.6	34.2	38.8	18.6	39.0
130-134	34.96287508845362	38.0	34.6	38.8	24.2	39.0
135-139	31.907738476472844	36.4	26.6	38.2	16.0	39.0
140-144	31.643712877042958	35.8	29.0	37.8	15.0	38.8
145-149	30.60172096165373	33.4	26.8	36.4	17.2	38.2
150-154	31.696419845985456	36.2	27.8	38.0	15.0	39.0
155-159	32.57040721490872	37.2	30.4	38.2	15.0	39.0
160-164	30.692835081357288	35.2	23.4	38.0	14.0	38.8
165-169	29.61093995878512	33.6	19.8	37.8	14.2	38.8
170-174	31.13451993990165	35.4	27.0	38.0	15.4	38.8
175-179	27.634419684112828	30.2	16.6	36.0	12.6	38.0
180-184	31.66124602585151	36.4	28.2	37.8	15.0	38.8
185-189	28.886326811820812	33.2	18.8	37.2	13.2	38.2
190-194	29.334548781294536	33.2	20.8	37.0	14.0	38.0
195-199	28.1850780371415	32.6	16.8	37.0	13.4	38.0
200-204	28.545687710609826	31.8	22.4	36.0	12.8	37.4
205-209	26.51349060537357	29.2	15.6	34.8	12.4	37.0
210-214	25.98867315059266	28.0	17.8	33.6	12.2	37.0
215-219	25.564569012867622	26.4	15.8	33.4	13.2	36.8
220-224	24.77951535685147	25.8	15.6	32.8	12.2	36.8
225-229	23.82504811075095	24.2	14.0	33.0	14.0	37.0
230-234	24.19305388504337	26.2	15.4	33.0	12.6	35.8
235-239	22.557024996542044	22.8	12.8	30.8	12.2	34.6
240-244	21.773481266375196	22.0	13.2	30.0	12.0	35.2
245-249	25.411542470767706	26.4	17.6	33.4	12.4	37.0
250-251	20.59680725655459	19.5	13.5	23.5	12.0	31.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31643467157811855
1101	2	0.21541429938663015
1101	3	0.4230352697124715
1101	4	0.6491672889186724
1101	5	0.5838793278220535
1101	6	0.47687551785494264
1101	7	0.4298505275463498
1101	8	0.7075187613468543
1101	9	0.3537871520811606
1101	10-14	0.28815668676886474
1101	15-19	0.5975422436245239
1101	20-24	0.601762569831223
1101	25-29	0.5530204465741946
1101	30-34	0.37404897155665395
1101	35-39	0.45479277639659443
1101	40-44	0.44641685949270027
1101	45-49	0.5249982979832666
1101	50-54	0.5324189265573693
1101	55-59	0.634792814939054
1101	60-64	0.46240294177867014
1101	65-69	0.6409350054463374
1101	70-74	0.3551561784556512
1101	75-79	0.46480574067618363
1101	80-84	0.7038646703406712
1101	85-89	0.4245697613816901
1101	90-94	0.9893476879993912
1101	95-99	0.49997720615984065
1101	100-104	0.6330672389488328
1101	105-109	0.9665327478324031
1101	110-114	0.5177066520030493
1101	115-119	0.5851319152762073
1101	120-124	0.6694859078696282
1101	125-129	0.8061702632085783
1101	130-134	0.6397774426650429
1101	135-139	1.0865835735429563
1101	140-144	1.1554636764668906
1101	145-149	1.1814071965782986
1101	150-154	1.2496186747606721
1101	155-159	1.005171781612347
1101	160-164	0.9186168508561536
1101	165-169	1.119433578135066
1101	170-174	1.1831520108461326
1101	175-179	1.3610213790637609
1101	180-184	0.6609257338621966
1101	185-189	1.795373443519832
1101	190-194	1.2780667379309385
1101	195-199	1.3401747839265745
1101	200-204	1.3474255927703034
1101	205-209	1.3560914174979182
1101	210-214	1.3630085081979963
1101	215-219	1.3052209355506044
1101	220-224	1.0631093336921928
1101	225-229	1.6408344333124099
1101	230-234	1.6031239778020705
1101	235-239	1.5324484482406895
1101	240-244	1.7897333018936372
1101	245-249	1.3992982440474329
1101	250-251	0.8031588025562826
1102	1	0.2221433750752304
1102	2	0.15214796770860772
1102	3	0.3341219564276514
1102	4	0.44387043282318217
1102	5	0.3704694059001774
1102	6	0.3463235054719931
1102	7	0.3540555129613523
1102	8	0.5586363842918161
1102	9	0.2588745913361379
1102	10-14	0.20658825791589663
1102	15-19	0.573460010877227
1102	20-24	0.29280494877116325
1102	25-29	0.27767717876688636
1102	30-34	0.09804546317450757
1102	35-39	0.2148191234365413
1102	40-44	0.4496304353574061
1102	45-49	0.3945146113778719
1102	50-54	0.44810897167266006
1102	55-59	0.5102468047954645
1102	60-64	0.31161508689659456
1102	65-69	0.41331119177619513
1102	70-74	0.5265864847724799
1102	75-79	0.4334012621235672
1102	80-84	0.47753520518012493
1102	85-89	0.33638040098012567
1102	90-94	0.5940341816051884
1102	95-99	0.25721218995169437
1102	100-104	0.26645515606011827
1102	105-109	0.4214665595199847
1102	110-114	0.19174430966089773
1102	115-119	0.456514766597536
1102	120-124	0.5728616188684441
1102	125-129	0.6812730619534832
1102	130-134	0.5753521535527
1102	135-139	0.8189507528463444
1102	140-144	0.6809572667118324
1102	145-149	0.3693100890074028
1102	150-154	0.8601152537785985
1102	155-159	0.8698482816941251
1102	160-164	0.8443659233978842
1102	165-169	1.4948360328378065
1102	170-174	0.9668313122851906
1102	175-179	1.0135045201728445
1102	180-184	0.2751698560199465
1102	185-189	1.5226135998498762
1102	190-194	1.4623393813488086
1102	195-199	1.1993954342988893
1102	200-204	1.2518108589860084
1102	205-209	1.294197083302084
1102	210-214	1.2128968987313016
1102	215-219	1.158248660815559
1102	220-224	0.6859956193801224
1102	225-229	1.0760976071173936
1102	230-234	1.2629877283206739
1102	235-239	0.8186314127072905
1102	240-244	1.7919880196158502
1102	245-249	1.5800609481354684
1102	250-251	0.8974778007788551
1103	1	0.07366919132807936
1103	2	0.06138192423790656
1103	3	0.08291505834108293
1103	4	0.25622971249433135
1103	5	0.2677055429036024
1103	6	0.2651106940364514
1103	7	0.23151398660156985
1103	8	0.4878096051676195
1103	9	0.2256731705453987
1103	10-14	0.15424031628915458
1103	15-19	0.1746008613872263
1103	20-24	0.09783083546599158
1103	25-29	-0.18762235660562965
1103	30-34	-0.026325432937319704
1103	35-39	-0.29727380171949136
1103	40-44	0.34908419150594483
1103	45-49	0.40356182851817834
1103	50-54	0.15466007709512297
1103	55-59	0.26946483065847815
1103	60-64	0.271343683834381
1103	65-69	-0.1830761198402584
1103	70-74	-0.09878041067489107
1103	75-79	0.3009649338393956
1103	80-84	0.19455211393360372
1103	85-89	0.19909410207502276
1103	90-94	0.4125531101996387
1103	95-99	0.09463430621936908
1103	100-104	-0.006104522349112074
1103	105-109	0.3339631856999574
1103	110-114	-0.09207842728600468
1103	115-119	0.2461201434418001
1103	120-124	0.34400039859889375
1103	125-129	0.5235983360675505
1103	130-134	0.40416184532041655
1103	135-139	0.5776627785088344
1103	140-144	0.3154292867470865
1103	145-149	0.2869609602651657
1103	150-154	0.4295771953948311
1103	155-159	0.4864623793662304
1103	160-164	0.3453402591379273
1103	165-169	0.6452306247079633
1103	170-174	0.6806064984341447
1103	175-179	0.6023459717753177
1103	180-184	-0.12102020628255161
1103	185-189	1.0659798112570975
1103	190-194	1.1135343937196254
1103	195-199	0.7347243950033047
1103	200-204	0.7958183480061827
1103	205-209	0.9407922035838467
1103	210-214	0.7567109446718554
1103	215-219	0.08418108015465009
1103	220-224	0.3942271301677529
1103	225-229	0.7311213706139554
1103	230-234	1.087732828042114
1103	235-239	0.4221980323371106
1103	240-244	0.7872888354796999
1103	245-249	0.9419609350291864
1103	250-251	0.7342476224871604
2112	1	-0.05267580716158449
2112	2	0.02671820477355169
2112	3	0.18654842978280683
2112	4	0.24524131095491697
2112	5	0.19497754408768841
2112	6	0.15785684686846224
2112	7	0.1409297141759538
2112	8	0.4098499369116837
2112	9	0.35046390046093023
2112	10-14	0.14059539551004008
2112	15-19	0.11832804321117862
2112	20-24	0.06475715094471468
2112	25-29	0.0917143684125179
2112	30-34	-0.11607211249403093
2112	35-39	0.16434489018936915
2112	40-44	0.1696125117791496
2112	45-49	0.10837396168712843
2112	50-54	0.17962725512881406
2112	55-59	0.18002734700998957
2112	60-64	0.24048552503163734
2112	65-69	0.06513777187846159
2112	70-74	0.17387349407338348
2112	75-79	0.1231940483765186
2112	80-84	0.254512000023837
2112	85-89	0.11679552901986057
2112	90-94	0.2243920145051561
2112	95-99	-0.008704449741749443
2112	100-104	-0.12869529691276682
2112	105-109	0.15667425952717906
2112	110-114	-0.36000855804863363
2112	115-119	0.12819621997734743
2112	120-124	0.3103269800076518
2112	125-129	0.4824527637663252
2112	130-134	0.3162677235539135
2112	135-139	0.10851645587915115
2112	140-144	0.2647511133616085
2112	145-149	-0.012782728360861029
2112	150-154	0.5264181232436265
2112	155-159	0.4678491960340523
2112	160-164	0.055167342235307615
2112	165-169	0.4714550933149759
2112	170-174	0.488569803592771
2112	175-179	0.6913828026143456
2112	180-184	-0.2654458730026228
2112	185-189	1.0576421962176212
2112	190-194	0.9576892005801589
2112	195-199	0.6480255731355946
2112	200-204	0.844744234127834
2112	205-209	0.6512499730130408
2112	210-214	0.3806558837402534
2112	215-219	0.2974550268121696
2112	220-224	0.39455429965252264
2112	225-229	0.6129236010379344
2112	230-234	0.6612779410174525
2112	235-239	0.5427998950477075
2112	240-244	1.2041641604827902
2112	245-249	1.0515160804541814
2112	250-251	0.5895108094708768
1113	1	-0.10219089223436129
1113	2	-0.04196734332744967
1113	3	0.03412349341473231
1113	4	0.09747974705020468
1113	5	0.1139025339517552
1113	6	0.10626303896055589
1113	7	0.06089534408419439
1113	8	0.20898199709883158
1113	9	-0.011431848029197056
1113	10-14	0.020591514595558635
1113	15-19	-0.02084658826545649
1113	20-24	-0.18925099273300106
1113	25-29	-0.019657702164472823
1113	30-34	1.2694427555924648
1113	35-39	0.17748502739503635
1113	40-44	0.16346026297384242
1113	45-49	0.059991034449282665
1113	50-54	0.062946530667368
1113	55-59	0.002615919214697726
1113	60-64	-0.21150167002706866
1113	65-69	-0.48210205152835073
1113	70-74	-0.5039576105204091
1113	75-79	0.13372320226355328
1113	80-84	0.23000217782053056
1113	85-89	0.029756741691429056
1113	90-94	0.09746090030066767
1113	95-99	-0.05823710136528604
1113	100-104	-0.20523618584770986
1113	105-109	-0.11614881822104905
1113	110-114	-0.5929635968086018
1113	115-119	-0.06825453910659718
1113	120-124	0.14897439884803987
1113	125-129	0.2462440458235733
1113	130-134	0.21314360869367732
1113	135-139	-0.029575528486329716
1113	140-144	-0.028584987342512136
1113	145-149	-0.4101082342993898
1113	150-154	0.13576180159098072
1113	155-159	0.16538237910599918
1113	160-164	0.00829373600692307
1113	165-169	0.48108889379329156
1113	170-174	0.09041630865721473
1113	175-179	0.20496222791378216
1113	180-184	-0.5351401540076637
1113	185-189	0.7946545163388805
1113	190-194	0.35741547740588686
1113	195-199	0.187873550482216
1113	200-204	-0.18862650581778695
1113	205-209	0.24195389171494952
1113	210-214	0.31368451756320326
1113	215-219	-0.19802172285907105
1113	220-224	-0.22165769821382497
1113	225-229	0.14485325637805957
1113	230-234	0.7451558489531251
1113	235-239	0.41317795726173046
1113	240-244	0.7601812895693278
1113	245-249	0.672538997880423
1113	250-251	0.3191667123675188
2113	1	-0.07145242788422834
2113	2	-0.02192441059425221
2113	3	0.09725521966309003
2113	4	0.09774877198541176
2113	5	0.09215628838035173
2113	6	0.0707019411838985
2113	7	-0.003056264396491315
2113	8	0.23747791495762982
2113	9	0.16775855508425508
2113	10-14	0.031077453307979397
2113	15-19	0.11412550932910648
2113	20-24	-0.2784637847187241
2113	25-29	-0.11746298036283065
2113	30-34	-0.3161361969079408
2113	35-39	0.03338305349395654
2113	40-44	0.09239479539711226
2113	45-49	0.012240943593880615
2113	50-54	-0.03811977202565231
2113	55-59	0.08084680191836924
2113	60-64	0.06276157167909702
2113	65-69	-0.11906113200792134
2113	70-74	-7.784190404294122E-4
2113	75-79	-0.030522847993601943
2113	80-84	0.09076869458681358
2113	85-89	0.028164282134532925
2113	90-94	-0.017329795447047047
2113	95-99	-0.09929868094019412
2113	100-104	-0.2993345186021301
2113	105-109	-0.14781672420734537
2113	110-114	-0.6816710570676676
2113	115-119	-0.23519282570187983
2113	120-124	0.1894256647396375
2113	125-129	0.2652839441160779
2113	130-134	0.19802800917633334
2113	135-139	-0.04645562646829049
2113	140-144	-0.3922162064668626
2113	145-149	-0.2844763571080158
2113	150-154	-0.033273963032609544
2113	155-159	0.013631441867403282
2113	160-164	-0.6209331466563377
2113	165-169	-0.5879038974386006
2113	170-174	0.12027050784621451
2113	175-179	0.04477531974470139
2113	180-184	-0.5578031652025892
2113	185-189	0.6592122002505398
2113	190-194	0.03907279413072118
2113	195-199	-0.2240555000832316
2113	200-204	0.4311170300825786
2113	205-209	0.2573501693153233
2113	210-214	0.07570155882658369
2113	215-219	-0.15102564813008712
2113	220-224	-0.29609407772920804
2113	225-229	0.24090981127882216
2113	230-234	0.6683560792254326
2113	235-239	-0.05971535511474002
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2106	95-99	-0.4054113298179445
2106	100-104	-0.3278323052662273
2106	105-109	-0.6034130832688511
2106	110-114	-0.23226703214974975
2106	115-119	-0.35260951289859577
2106	120-124	-0.21669852999393413
2106	125-129	-0.3499797305024046
2106	130-134	-0.3997297073647985
2106	135-139	-0.699580009180707
2106	140-144	-0.6807104284425556
2106	145-149	-0.1759668878938747
2106	150-154	-0.8861202965089703
2106	155-159	-0.7616687049496633
2106	160-164	-0.3775583930530111
2106	165-169	-0.6329738223723638
2106	170-174	-0.7694242292042084
2106	175-179	-0.8497502808039066
2106	180-184	-0.12382147587749515
2106	185-189	-0.5663895787426263
2106	190-194	-1.009859943072609
2106	195-199	-0.9022543450404719
2106	200-204	-0.8897959971152822
2106	205-209	-0.8224052910706234
2106	210-214	-0.9404672070384485
2106	215-219	-1.013458794080968
2106	220-224	-0.7001590365314208
2106	225-229	-0.9166992105448273
2106	230-234	-1.0275544939423469
2106	235-239	-0.8059307791813666
2106	240-244	-0.5210516732003541
2106	245-249	-0.639492961179787
2106	250-251	-0.6711227043058052
2105	1	-0.3146629681596309
2105	2	-0.227205182451236
2105	3	-0.5198906484271504
2105	4	-0.7492070394501731
2105	5	-0.6368119481335697
2105	6	-0.5125105709917079
2105	7	-0.31524863570216866
2105	8	-1.008549815286358
2105	9	-0.5862205248201917
2105	10-14	-0.26395444090270104
2105	15-19	-0.4031621393090532
2105	20-24	-0.5063278154355046
2105	25-29	-0.3723592680891983
2105	30-34	-0.7000369713533274
2105	35-39	-0.2435429388307071
2105	40-44	-0.39322572577334114
2105	45-49	-0.3819785564887326
2105	50-54	-0.45061754939282395
2105	55-59	-1.7643696517460654
2105	60-64	-0.41732422410662906
2105	65-69	-0.46462737166596924
2105	70-74	-0.12194852258298283
2105	75-79	-0.39162873882064275
2105	80-84	-0.49612976187653146
2105	85-89	-1.0902261188617786
2105	90-94	-1.1088840125251807
2105	95-99	-0.5150274137828674
2105	100-104	-0.806795159295298
2105	105-109	-0.6869165888555635
2105	110-114	-0.17130290471290266
2105	115-119	-0.24404690661454254
2105	120-124	-0.10266389197828829
2105	125-129	-0.452917317448275
2105	130-134	-0.5154858615495144
2105	135-139	-1.006184044949702
2105	140-144	-1.0359231862985645
2105	145-149	-0.6295233550182395
2105	150-154	-1.0672282666762882
2105	155-159	-0.8965241363114629
2105	160-164	-0.2368222735230816
2105	165-169	-0.6048986187803145
2105	170-174	-1.0426971137092629
2105	175-179	-1.0410111936037865
2105	180-184	-0.24999557728611776
2105	185-189	-0.7168962572724418
2105	190-194	-0.9251615704998919
2105	195-199	-1.1022161200153313
2105	200-204	-1.2886760496086787
2105	205-209	-1.1629989625594774
2105	210-214	-1.1653340860226926
2105	215-219	-1.2131264940835749
2105	220-224	-0.7433779071005269
2105	225-229	-0.8795302879795415
2105	230-234	-1.2035458822891236
2105	235-239	-0.5699138538373774
2105	240-244	-2.652600852838578
2105	245-249	-0.7739909279564365
2105	250-251	-0.5095152961090044
2104	1	-0.14590039405451805
2104	2	-0.0973836296757824
2104	3	-0.22102657605750053
2104	4	-0.2839835312023844
2104	5	-0.20839325059913705
2104	6	-0.1846744772697022
2104	7	-0.16849948594478548
2104	8	-0.5012947259122669
2104	9	-0.36565547966604584
2104	10-14	-0.1264569676309435
2104	15-19	-0.3011175493545437
2104	20-24	-0.34285028068479306
2104	25-29	-0.15931477084736656
2104	30-34	-0.18682320024570487
2104	35-39	-0.19439473248703365
2104	40-44	-0.2545988852870522
2104	45-49	-0.0738134398241641
2104	50-54	-0.3026312753082365
2104	55-59	-0.7540952869741275
2104	60-64	-0.26127399627459624
2104	65-69	-0.21618460100017245
2104	70-74	-0.20245579206044795
2104	75-79	-0.3630432010021991
2104	80-84	-0.4877191687106972
2104	85-89	-0.2923076430327711
2104	90-94	-0.4297328780379459
2104	95-99	-0.26265954179725526
2104	100-104	-0.10303658663283954
2104	105-109	-0.09696025564345234
2104	110-114	0.05552910903145403
2104	115-119	-0.23470933962788365
2104	120-124	-0.15120385540516423
2104	125-129	-1.76096216328137
2104	130-134	-0.4204747194693894
2104	135-139	-0.5639751710759384
2104	140-144	-0.22416624698410814
2104	145-149	-0.5778524125014428
2104	150-154	-0.7031732965850033
2104	155-159	-0.4295610372641363
2104	160-164	-0.11401076941358212
2104	165-169	-0.23664808971895823
2104	170-174	-0.6021252383625892
2104	175-179	-0.21465320921649678
2104	180-184	0.2807867704880529
2104	185-189	-0.2838803116280353
2104	190-194	-0.8868292432136222
2104	195-199	-0.641653580613994
2104	200-204	-0.8095155823465703
2104	205-209	-0.8873485616587686
2104	210-214	-0.7889332555335891
2104	215-219	-0.2583659748986733
2104	220-224	-0.1298875644994837
2104	225-229	-0.522333912822571
2104	230-234	-0.8211721833286703
2104	235-239	-0.29636056985475534
2104	240-244	-2.6747290878643
2104	245-249	-2.770469979333498
2104	250-251	-0.7067938967526182
2111	1	-0.040931956371366596
2111	2	-0.05815020536454085
2111	3	-0.11794409388037863
2111	4	-0.1230156412592116
2111	5	-0.23825605298126362
2111	6	-0.19384019502030725
2111	7	-0.12591366103411872
2111	8	-0.370270598977271
2111	9	-0.28616278475708157
2111	10-14	-0.06472968406193758
2111	15-19	-0.053846949345839334
2111	20-24	-0.08174839467874051
2111	25-29	0.09279742951530778
2111	30-34	-0.08527875250712214
2111	35-39	-0.08852150999792485
2111	40-44	-0.25002027292222806
2111	45-49	-0.07332273097151898
2111	50-54	-0.15294721536636047
2111	55-59	-0.024592263344956677
2111	60-64	-0.09538492479384786
2111	65-69	0.12333605281235549
2111	70-74	0.07173148548287145
2111	75-79	-0.0012056370281214868
2111	80-84	-0.14548306447704817
2111	85-89	-0.21517098711544236
2111	90-94	-0.3834388801366373
2111	95-99	-0.131855008651236
2111	100-104	0.11481285904216065
2111	105-109	-0.08676658279562588
2111	110-114	0.3038012290122367
2111	115-119	0.03027819013089328
2111	120-124	0.15060311689369144
2111	125-129	-0.5176194894438169
2111	130-134	-0.14958389432036512
2111	135-139	-0.0013309319127117192
2111	140-144	0.1627524212577427
2111	145-149	0.20282746017448972
2111	150-154	-0.1803683448830249
2111	155-159	-0.23771266216087383
2111	160-164	-0.3367214575509081
2111	165-169	-0.3141322935853026
2111	170-174	-0.310074300839581
2111	175-179	-0.2948759883000065
2111	180-184	0.4597456783158229
2111	185-189	-0.04747577705587247
2111	190-194	-0.43105214873397557
2111	195-199	-0.4364224180123131
2111	200-204	-0.422363573456245
2111	205-209	-0.5373592056620922
2111	210-214	-0.4993155162722722
2111	215-219	-0.23208473877360447
2111	220-224	-0.4113052784043525
2111	225-229	-0.7841005027555887
2111	230-234	-0.41109132158504025
2111	235-239	-0.0782876806091437
2111	240-244	-1.9796357254699757
2111	245-249	-0.3202910509249932
2111	250-251	-0.4726000108812869
2110	1	0.14998667008376643
2110	2	0.05811919796448706
2110	3	0.016674245832074774
2110	4	-0.0711359854568343
2110	5	-0.08070371368294715
2110	6	0.005364500238158598
2110	7	0.0055051985092475775
2110	8	-0.21832085678598645
2110	9	-0.2860859904334063
2110	10-14	0.0647286065181234
2110	15-19	-0.8834721631807838
2110	20-24	-0.04574448165659106
2110	25-29	0.04223349747424976
2110	30-34	0.19667014622628898
2110	35-39	0.013691904454056214
2110	40-44	-0.10028291690611013
2110	45-49	-0.06157938699498544
2110	50-54	-0.07794518563962072
2110	55-59	0.021615059186807173
2110	60-64	0.020289311500775398
2110	65-69	0.11439283942176814
2110	70-74	0.3815836629298559
2110	75-79	-0.010122188496353601
2110	80-84	-0.10942663745198189
2110	85-89	0.09490580456895259
2110	90-94	0.24882565356373476
2110	95-99	0.21649935829086786
2110	100-104	0.2643890468196801
2110	105-109	-0.006585598193950659
2110	110-114	0.3689617637718925
2110	115-119	0.363216006217705
2110	120-124	0.3463258079808469
2110	125-129	0.09461869182434413
2110	130-134	-0.11610757911395808
2110	135-139	0.10452512483616871
2110	140-144	0.3840885939755516
2110	145-149	0.20186240016272805
2110	150-154	-0.06014810323561548
2110	155-159	-0.12864576297514674
2110	160-164	-0.2084067672303611
2110	165-169	0.16750606259964584
2110	170-174	0.03811601705669432
2110	175-179	-0.17796066165843527
2110	180-184	0.7799281524082353
2110	185-189	-0.08994852861463443
2110	190-194	-0.3830611548830447
2110	195-199	-0.5814664597748553
2110	200-204	-0.16932399085203187
2110	205-209	-0.34964080479859305
2110	210-214	-0.0849387555587704
2110	215-219	0.03289548759530092
2110	220-224	0.09832310037702285
2110	225-229	-0.3294973828942922
2110	230-234	-2.3231400939869644
2110	235-239	-0.6141614612246968
2110	240-244	-1.5706833640274205
2110	245-249	0.03763209520087685
2110	250-251	-0.08726356558504378
2109	1	0.11830203376501203
2109	2	0.06213410331270808
2109	3	0.10013089431376088
2109	4	0.026900381403311968
2109	5	-0.05556990505025183
2109	6	0.06114516774058387
2109	7	0.08289618988149527
2109	8	-0.23055230355620893
2109	9	-0.13312474210479763
2109	10-14	0.14228003111742282
2109	15-19	-1.1927341730276098
2109	20-24	-0.13100364899016625
2109	25-29	-0.007457977495889168
2109	30-34	0.1898539898923559
2109	35-39	0.043200612058811316
2109	40-44	-0.22688284869838782
2109	45-49	-0.0922858695206159
2109	50-54	-0.06869390828472888
2109	55-59	0.08764663227908187
2109	60-64	0.03826931251668242
2109	65-69	0.2765940292798419
2109	70-74	0.21803002260144666
2109	75-79	-0.11039726703746311
2109	80-84	-0.022571402407571384
2109	85-89	0.10689496698677203
2109	90-94	0.31962104864982877
2109	95-99	0.15246295719454395
2109	100-104	0.33975473460214545
2109	105-109	-0.025259043395315928
2109	110-114	0.4255460566907061
2109	115-119	0.19807107807231716
2109	120-124	0.28494257453661476
2109	125-129	0.050708837248663485
2109	130-134	-0.06445986654669156
2109	135-139	-0.023861459263624596
2109	140-144	0.031381803458355506
2109	145-149	-0.17206460912631982
2109	150-154	-0.2930960249532433
2109	155-159	-0.23139167950134976
2109	160-164	0.0036653174726524185
2109	165-169	-0.5607269800491181
2109	170-174	-0.08543053174304305
2109	175-179	-0.04890039265236368
2109	180-184	0.8119766993324937
2109	185-189	0.03680031451489896
2109	190-194	-0.3049818089252305
2109	195-199	-0.43049526357673784
2109	200-204	-0.16857089618437726
2109	205-209	-0.18601779307057953
2109	210-214	-0.06615035834666116
2109	215-219	0.1784031584913599
2109	220-224	0.08099899792395249
2109	225-229	-0.16361212337460174
2109	230-234	-0.27833454962098614
2109	235-239	0.31752890826686553
2109	240-244	-0.0068199824152088695
2109	245-249	0.04464180009215468
2109	250-251	-0.10340228797906903
2108	1	0.19385916320253926
2108	2	0.11373394279817006
2108	3	-0.0803247344601381
2108	4	-0.040471406526020104
2108	5	-7.282606545402359E-4
2108	6	0.06965661302879766
2108	7	0.07323512047905467
2108	8	-0.22874473483043545
2108	9	-0.190382909311694
2108	10-14	0.17533929618927147
2108	15-19	0.10809584926929006
2108	20-24	0.2658832578788193
2108	25-29	-0.07590058131694377
2108	30-34	1.1985452850285867
2108	35-39	0.21337196283334947
2108	40-44	-0.13643671055939421
2108	45-49	0.026681918976031227
2108	50-54	0.05200184763086213
2108	55-59	0.02982124972663769
2108	60-64	0.002115770032261821
2108	65-69	0.2731219809087051
2108	70-74	0.5847112531620269
2108	75-79	-0.01260045796683329
2108	80-84	-1.4145953098795019
2108	85-89	0.07697984359848675
2108	90-94	0.27690632414321925
2108	95-99	0.12530760000206698
2108	100-104	0.29154390998910884
2108	105-109	0.09492020809096147
2108	110-114	0.5002411455827414
2108	115-119	0.290478259609813
2108	120-124	0.3458615378617438
2108	125-129	-0.05460887305207507
2108	130-134	-0.17149269823827495
2108	135-139	-0.15916523178462327
2108	140-144	0.018678147555803548
2108	145-149	-0.21593125796445634
2108	150-154	-0.0332490384509434
2108	155-159	-0.12117903149011511
2108	160-164	-0.07611749396465939
2108	165-169	-0.7507353451797236
2108	170-174	0.007322436712598801
2108	175-179	0.3399030825079876
2108	180-184	0.9047840907708213
2108	185-189	0.16666682819329282
2108	190-194	-0.4347521706880286
2108	195-199	-0.6928031915320965
2108	200-204	-0.11376477003618035
2108	205-209	-0.16030418991251594
2108	210-214	0.10896884372493432
2108	215-219	-0.06972298755471584
2108	220-224	0.20011947659730467
2108	225-229	0.11399130634684695
2108	230-234	-0.08065347576492954
2108	235-239	0.005358389484634074
2108	240-244	0.1303460696388079
2108	245-249	0.03733935016216705
2108	250-251	-0.07926752603930609
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	8.0
13	39.0
14	271.0
15	1089.0
16	7393.0
17	26782.0
18	45198.0
19	68364.0
20	98394.0
21	118236.0
22	137933.0
23	189632.0
24	275993.0
25	398836.0
26	549518.0
27	722417.0
28	905520.0
29	1088478.0
30	1267266.0
31	1475282.0
32	1716403.0
33	1931691.0
34	2003708.0
35	1767232.0
36	1031969.0
37	135496.0
38	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.9437056604303105	2.5648542945861372	2.7038179388953374	92.78762210608822
2	2.1452571319176745	3.050415807188147	2.1583998865388123	92.64592717435536
3	2.537071588797412	2.5353488731058897	72.12815776918467	22.799421768912033
4	90.34867013865919	3.3070440654386397	3.9195728567161607	2.4247129391860063
5	2.579011885360099	3.2147942062628174	92.25263431419127	1.9535595941858157
6	1.8270057672762698	2.370362825223665	92.9968913438736	2.805740063626468
7	2.3897073563342817	2.319195036975117	79.10748041992667	16.18361718676393
8	92.70122948026356	2.3604963626267668	3.008093380962977	1.9301807761466898
9	1.846300183021315	11.768365778021005	2.747048706121492	83.63828533283619
10-14	2.171287107202959	3.3723858164591345	20.7184218250017	73.73790525133622
15-19	21.912967400957005	18.71188851736698	26.63728528071621	32.737858800959806
20-24	21.184050684676127	3.2809815695731577	44.01212215520127	31.522845590549448
25-29	18.721718646323836	8.830116929170952	25.088670369189874	47.35949405531534
30-34	37.82784093984352	21.040208309528534	23.536909465589787	17.595041285038157
35-39	23.010720679003065	33.83036750599881	21.28964375993537	21.869268055062754
40-44	32.89281300553875	16.93365001232838	27.79702500155358	22.376511980579295
45-49	26.638271300533827	24.710564970562235	9.89334072033822	38.757823008565715
50-54	28.910176225671496	21.187378344950076	14.500128044759036	35.402317384619394
55-59	19.917472521745058	2.675470182359792	41.65014112486945	35.7569161710257
60-64	20.05139831893018	20.537152775689638	4.349677958660838	55.06177094671935
65-69	36.51963068984803	7.4364825327904835	30.641380207237063	25.402506570124427
70-74	18.456896525802396	3.8998737593941257	43.419350093916194	34.22387962088728
75-79	18.829915214824524	12.085701284356588	27.690987152424885	41.393396348394
80-84	2.429659325767918	35.17868633594535	38.73355965432425	23.65809468396248
85-89	21.547642673434243	23.711285880095982	20.718495226010276	34.0225762204595
90-94	2.1039294956990995	20.10881173461308	56.01863740330241	21.768621366385418
95-99	15.891609986188204	21.019940402807265	8.261768591297166	54.826681019707365
100-104	1.954628304356629	37.19319623773927	21.8583028130757	38.99387264482839
105-109	39.631593177494594	26.584788182395876	11.936898740901555	21.84671989920798
110-114	3.382222125332515	18.862600456250288	39.635726442270105	38.11945097614709
115-119	12.77574351156362	25.122974336923598	25.138969908729898	36.962312242782886
120-124	2.264001731987034	3.553015291200343	34.044558072683316	60.13842490412931
125-129	18.16019249011465	17.854704252372766	18.329564887490566	45.65553837002202
130-134	4.121392445409333	24.533484832083456	28.924252378976405	42.42087034353081
135-139	22.426073852333996	28.898148899994645	15.616757470907498	33.05901977676386
140-144	33.146484457220524	25.565008148758444	11.53986727446582	29.748640119555215
145-149	8.658598451868682	19.119718831747168	45.01026419079551	27.211418525588638
150-154	32.08509153176978	24.872097128491	14.163746371193318	28.879064968545904
155-159	14.054082497910189	22.771052713346123	33.92138452718157	29.253480261562114
160-164	41.02064577657768	18.068578868555417	8.435864359756158	32.47491099511075
165-169	30.100688033853935	27.065787943846736	38.64220811307631	4.191315909223031
170-174	27.496142714229943	10.603742447661297	10.369978069139886	51.53013676896887
175-179	31.199287399584936	18.046697473166947	39.168181894323006	11.58583323292511
180-184	5.058485947883101	29.72150678012669	21.83923669586434	43.380770576125876
185-189	21.748940354419382	29.13731357428083	18.187530650245133	30.926215421054653
190-194	11.572917105176115	20.93977085250246	36.717578866550795	30.769733175770625
195-199	22.187424478492296	33.86770513055686	9.465954495573241	34.47891589537761
200-204	15.972097769064387	7.041196333960778	20.069715485046576	56.91699041192826
205-209	29.82366194258761	27.558978613254947	6.333923270542831	36.28343617361461
210-214	9.437345004133139	3.5209941665963593	37.99573087236145	49.04592995690905
215-219	17.901099001191955	22.865039929356374	21.846948745272194	37.38691232417947
220-224	14.876086186352062	24.48876594531569	19.9391802421572	40.69596762617505
225-229	6.043970947770679	36.13052256784578	33.91106203647279	23.914444447910757
230-234	24.356184491150938	27.74775818619159	11.914845623561007	35.98121169909646
235-239	18.478867102068964	34.97480074130649	4.457707893571	42.088624263053546
240-244	21.80595633405545	13.764920769673298	28.825333778675184	35.603789117596065
245-249	3.14130883219808	10.407595915893335	22.283146080817367	64.16794917109122
250-251	29.416034461329982	3.8176225420918954	12.798665653333973	53.96767734324415
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	25.0
1	6.666666666666664
2	1.3333333333333333
3	1.3333333333333333
4	1.6666666666666665
5	1.6666666666666665
6	1.6666666666666665
7	1.0
8	0.6666666666666666
9	1.0
10	1.6666666666666665
11	2.333333333333333
12	1.3333333333333333
13	1.3333333333333333
14	1.9999999999999998
15	1.6666666666666665
16	1.0
17	0.6666666666666666
18	3.3333333333333335
19	3.666666666666667
20	3.0
21	3.3333333333333335
22	4.999999999999999
23	8.666666666666664
24	16.666666666666675
25	19.999999999999996
26	35.66666666666664
27	61.000000000000156
28	98.66666666666634
29	159.66666666666683
30	297.6666666666672
31	562.9999999999941
32	1060.0000000000382
33	1640.6666666665728
34	1958.3333333331673
35	2533.000000000034
36	4573.000000000311
37	8086.999999997115
38	13686.333333340346
39	24697.999999981672
40	38333.33333332614
41	48436.666666732985
42	57772.00000013424
43	73142.00000008005
44	98199.99999971541
45	128739.99999927099
46	152259.9999998537
47	158229.6666666941
48	151879.99999984264
49	162045.66666680516
50	214335.666668327
51	240573.33333575728
52	375414.66666312516
53	556539.6666582142
54	735691.6666790702
55	810331.0000210927
56	791852.0000189415
57	739866.6666795562
58	650609.0000024986
59	530165.9999884772
60	392322.9999954743
61	264033.00000294176
62	164731.33333355
63	138571.99999945532
64	112204.99999951161
65	53982.66666677334
66	25805.66666664431
67	14259.666666674722
68	8797.666666664787
69	5307.3333333329765
70	3446.333333333783
71	2267.9999999999136
72	1493.333333333273
73	1045.3333333333749
74	765.3333333333504
75	528.6666666666569
76	389.66666666666197
77	300.0000000000004
78	244.3333333333359
79	193.33333333333445
80	166.33333333333368
81	144.6666666666664
82	123.33333333333266
83	105.33333333333292
84	102.99999999999962
85	94.33333333333307
86	79.99999999999994
87	80.6666666666666
88	74.33333333333336
89	75.33333333333334
90	79.99999999999994
91	75.00000000000001
92	75.33333333333334
93	73.6666666666667
94	75.66666666666667
95	72.33333333333339
96	84.66666666666654
97	87.33333333333317
98	104.66666666666626
99	216.3333333333351
100	696.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	2.5057682785772853E-6
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	1.2528841392886426E-6
130-134	0.0
135-139	1.2528841392886426E-6
140-144	0.0
145-149	1.2528841392886426E-6
150-154	1.2528841392886426E-6
155-159	0.0
160-164	0.0
165-169	0.0
170-174	1.2528841392886426E-6
175-179	1.2528841392886426E-6
180-184	1.2528841392886426E-6
185-189	2.5057682785772853E-6
190-194	0.0
195-199	1.2528841392886426E-6
200-204	0.0
205-209	1.2528841392886426E-6
210-214	2.5057682785772853E-6
215-219	3.758652417865927E-6
220-224	1.2528841392886426E-6
225-229	3.758652417865927E-6
230-234	1.2528841392886426E-6
235-239	0.0
240-244	1.2528841392886426E-6
245-249	1.2528841392886426E-6
250-251	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
251	1.5963168E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.607048804432779
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.03820886857358	12.12943169575937
2	7.63678309175311	2.2310172666020947
3	2.590841064015054	1.1353362559978932
4	1.5779257535238116	0.9219535396597475
5	0.8420363477501811	0.6149833013346614
6	0.5579137655252725	0.4889684161015513
7	0.42472011100263224	0.43427351727476887
8	0.29653075580019694	0.34651513775910525
9	0.2846629505117627	0.3742277049845236
>10	1.9785189247850654	5.837857160461701
>50	0.3717006301443748	3.9415436019354857
>100	0.3162281712937974	8.343785775558374
>500	0.03318739217544097	3.4370775648174994
>1k	0.03546308506229452	10.988253117308144
>5k	0.006242451237813658	6.287041678759767
>10k+	0.009036636845592014	42.487734265685546
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAATACCGGTCCAGGA	251043	1.5726389648971932	No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGAACAGGCCCAGAG	226170	1.4168240289145615	No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCATCAGCGTCAGTTATGGTCCAGTG	194725	1.2198393201149045	No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCAGTTGTGGTCCAGTG	154981	0.9708661839554655	No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGTGTCAGTATTGGGCCAGGA	139956	0.8767432629914063	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG	111282	0.6971172639415936	No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCAGTAATGGTCCAGTG	109334	0.6849141724249221	No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT	107206	0.671583485182891	No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG	99129	0.6209857592177191	No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA	88650	0.5553408947396907	No Hit
CCTGTTTGCTCCCCACGCTGTCGCACCTGAGTGTCAGTATTGGGCCAGGT	88484	0.5543010009040812	No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG	83250	0.5215130229788975	No Hit
CCTGTTTGATCCCCACGCTGTCGCGCCTGAGCGTCAGTTACGAGCCAGTG	78598	0.4923709378990436	No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAATGGCCCAGAG	78039	0.4888691267297319	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTACTGGTCCAGGA	77089	0.4829179270681108	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG	74575	0.46716917343725256	No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCATCAGCGTCAGTTGTGGTCCAGTG	73259	0.4589251958007333	No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGGAAATGTCCAGAG	72973	0.45713357148155054	No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGTG	72243	0.452560544373147	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTGACCCAGGG	68730	0.430553634466542	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCCAACCAGGG	67858	0.4250910596192435	No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCATCAGCGTCAGTAATGGTCCAGTG	66397	0.41593874098173994	No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAATGCCGGTCCAGGA	66012	0.4135269390136093	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGGCCCAGAA	65891	0.41276894410933973	No Hit
CCTGTTTGATCCCCACGCTATCGCGCCTGAGCGTCAGATACGAGCCAGTG	64012	0.4009980976207229	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGGTCCAGTT	63907	0.40034033344759634	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG	63754	0.39938187708104056	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTCAAGGACCAGGT	59830	0.3748002902681974	No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAATGCCGGTCCAGGA	58429	0.36602383687248047	No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACTCCTTAGTA	58355	0.3655602697409437	No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCAGTTATGGTCCAGTG	55588	0.34822661767388524	No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA	54648	0.34233806221922863	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGATCGTCAGTTCTGGTCCAGGA	52972	0.3318388931319899	No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG	52184	0.32690252962319255	No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATTGGTCCAGGA	52091	0.3263199384984234	No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA	51549	0.32292462248095116	No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG	49427	0.30963152176309866	No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAGTGTTGGTCCAGGA	48721	0.30520884075140975	No Hit
CCTGTTTGATCCCCACGCTGTCGCGCCTGAGCGTCAGAAATGAGCCAGTG	44157	0.2766180246928429	No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA	43304	0.27127447383877684	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG	41480	0.2598481704884644	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGTTGGTCCAGGT	41426	0.2595098917708565	No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAATGCCGGTCCAGGA	41086	0.2573799887340658	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG	39514	0.2475323193992571	No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAACGTTCCAGAG	39497	0.24742582424741758	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCAGCCCAGAG	39311	0.24626064199787914	No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG	38636	0.24203215802777994	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGGCCCAGGA	38311	0.2399962213014359	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGCG	37099	0.23240374341734674	No Hit
CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGAG	36804	0.230555739311896	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG	36379	0.22789336051590764	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG	35862	0.22465465501584647	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGATGGTCCAGTT	35417	0.22186698780592923	No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT	35247	0.2208020362875339	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTATCAGCGTCAGTATCCAACCAGGG	35114	0.21996886833490695	No Hit
CCTGTTTGCTCCCCATGCTTTCGCACCTCAGCGTCAGTATTAGGCCAGAA	34972	0.21907932059601204	No Hit
CCTGTTTGCTCCCCACGCTGTCGCACCTGAGTGTCAGTATTGGGCCAGGC	33335	0.20882446391593448	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTGCTGGTCCAGGA	32995	0.20669456087914378	No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGAACAGGTCCAGAG	32864	0.20587392176790975	No Hit
CCTGTTTGATCCCCACGCTGTCGCGCCTGAGCGTCAGAAACGAGCCAGTG	32494	0.20355608611022571	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTAACGTCCCAGCG	32472	0.20341826885490402	No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGAGTTGTCCAGGG	32173	0.20154520706666748	No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG	31287	0.1959949303296188	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACTGTCCAGAA	31215	0.19554389203947486	No Hit
CCTGTTTGATCCCCACGCTGTCGCGCCTGAGCGTCAGTAATGAGCCAGTG	30578	0.19155345605584054	No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGCCCAGCA	30256	0.18953631259158582	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGGACCAGGT	30166	0.18897251472890594	No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTAACAGCCCAGAG	29776	0.1865293906572931	No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCGGTATCTGTCCAGAA	29547	0.1850948383178076	No Hit
CCTGTTTGCTCCCCACGCTTTCGCACTTGAGTGTCAGTACTGGACCAGAG	29491	0.18474403075880677	No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA	29081	0.18217561827326506	No Hit
TACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC	28898	0.18102922928581594	Illumina Single End PCR Primer 1 (100% over 21bp)
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTACCGGCCCAGAG	28859	0.18078491687865467	No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAGTGTTGGTCCAGGA	28254	0.1769949423573065	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG	28237	0.176888447205467	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTATCAGCGTCAGTATCCAACCAGGT	27984	0.17530354876926685	No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA	27886	0.1746896355410154	No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGAG	27872	0.1746019336512652	No Hit
CCCTTTTGCTCCCCATGCTTTCGTGCCTGAGCGTCAGTAACTGTCCAGGT	27782	0.17403813578858532	No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAATACAGGTCTAGCC	27334	0.17123167531657876	No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG	26698	0.16724750375364086	No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGAACCAGGA	26581	0.16651456653215702	No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTACCGGGCCAGTG	26405	0.16541202848958303	No Hit
CCTGTTTGCTCCCCCGGCTTTCGTGCCTCAGTGTCAGGTGTTGTCCAGGG	25587	0.16028773235989247	No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG	25345	0.1587717425513532	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCATCGTCCAGAA	25309	0.15854622340628127	No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGTGTCAATATTCGTGTAGCC	24622	0.15424256638782477	No Hit
CCTGTTTGATCCCCACGCTGTCGCGCCTGAGCGTCAGTGATGAGCCAGTG	24439	0.15309617740037568	No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCAGCCTAGCA	24240	0.1518495576817835	No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGTGTCAGCAACGGCCCAGCA	23995	0.1503147746111549	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATTACGGGCCAGAT	23901	0.14972591906568922	No Hit
CCCTTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGCAATTGTCCAGGT	23623	0.147984410112078	No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACTTGTCCAGCG	23247	0.14562898793021536	No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG	23133	0.14491484397082083	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTGCTGGACCAGGA	22895	0.14342391184506734	No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTTGGCCCAGCA	22794	0.14279120535472659	No Hit
CCTGTTTGATCCCCACGCTGTCGTGCATGAGCGTCAGTTACGAGCCAGTG	22672	0.1420269460297605	No Hit
TACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC	22434	0.14053601390400702	Illumina Single End PCR Primer 1 (100% over 21bp)
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTAATGCCCAGAG	22411	0.14039193222798882	No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACAGCCCAGCA	22347	0.13999100930341646	No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTGAGCGTCGGTAACGGGCCAGTG	22168	0.13886967799875313	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG	22021	0.13794880815637597	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTTGATCCAGAT	21961	0.13757294291458938	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTCCGGTCCAGTG	21829	0.1367460393826589	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGGG	21501	0.13469130939422552	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGA	21415	0.13415256921433139	No Hit
CCTGTTTGATCCCCACGCTATCGCGCCTGAGCGTCAGTAATGAGCCAGTG	21216	0.1329059494957392	No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGTG	20720	0.12979879683030338	No Hit
CCTGTTTGCTCCCCCAGCTTTCGTGCATCAGCGTCAGTTATGGTCCAGTG	20540	0.1286712011049436	No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTTATGGTCCAGTG	20210	0.12660394227511731	No Hit
CCTGTTTGATCCCCACGCTGTCGTGCATGAGCGTCAGAAACGGGCCAGTG	20079	0.12578330316388328	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGTGTCAGTGTTGGCCCAGGA	19141	0.11990727655061953	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAGGT	19049	0.11933094984654675	No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGATCCAGTG	18830	0.11795904171402569	No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAATATTGGTCCAGGA	18697	0.11712587376139874	No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAGTATTGGTCCAGGA	18647	0.11681265272657658	No Hit
CCCGTTTGCTCCCCTGGCTTTCGTTCCTCAGTGTCAGTTGCAGTCCAGAA	18621	0.11664977778846905	No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAATGCCGGTCCAGGA	18249	0.11431941328939217	No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGTGTTGTCCAGGG	18178	0.11387463941994472	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGGTCCAGAT	18018	0.1128723321085138	No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATTGGCCCAGGA	17817	0.11161318354852871	No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGG	17805	0.1115380105001714	No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGTAATGGTCCAGTG	17636	0.11047932340247249	No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGCTTCAGCGTCAGTGTCAGCCCAGCA	17454	0.10933919883571984	No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA	17257	0.10810510795852052	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGTCCAGAG	17220	0.10787332439275212	No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA	17169	0.10755383893723351	No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCAGTCCAGTG	17090	0.10705894970221451	No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACTCTTTAGTA	16878	0.10573089251456853	No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAATCCAGGTCTAGCC	16764	0.10501674855517401	No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG	16726	0.10477870056870918	No Hit
CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGGAACTGTCCAGAG	16432	0.10293696088395488	No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGGTAAGGCCCAGAG	16345	0.10239195628336431	No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG	16217	0.10159011043421957	No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTACAGGCCCAGAG	16207	0.10152746622725516	No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCCAACCAGGT	16097	0.10083837995064639	No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACATGTCCAGTG	15990	0.10016808693612696	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	5.637978626798891E-4	0.0	0.0
2	5.637978626798891E-4	0.0	0.0
3	5.700622833763324E-4	0.0	0.0
4	5.700622833763324E-4	0.0	0.0
5	5.763267040727755E-4	0.0	0.0
6	5.825911247692188E-4	0.0	0.0
7	5.951199661621051E-4	0.0	0.0
8	5.951199661621051E-4	0.0	0.0
9	6.076488075549915E-4	0.0	0.0
10-14	6.427295634550735E-4	0.0	0.0
15-19	6.90339160748042E-4	0.0	0.0
20-24	7.554891359910513E-4	0.0	0.0
25-29	8.23144879512638E-4	0.0	0.0
30-34	9.308929154914613E-4	0.0	0.0
35-39	0.0010912620853204076	0.0	0.0
40-44	0.0013518619862924453	0.0	0.0
45-49	0.0018091646971327997	0.0	0.0
50-54	0.0024832163640700895	0.0	0.0
55-59	0.0033514650725971185	0.0	0.0
60-64	0.004541705004921329	0.0	0.0
65-69	0.005858486235313692	0.0	0.0
70-74	0.007437120250817381	0.0	0.0
75-79	0.009387860855689798	0.0	0.0
80-84	0.011684397483005881	0.0	3.7586524178659275E-6
85-89	0.014290396492726254	0.0	6.264420696443212E-6
90-94	0.017501538541723045	0.0	6.264420696443212E-6
95-99	0.02139174379421428	0.0	6.264420696443212E-6
100-104	0.026009874731632215	0.0	6.264420696443212E-6
105-109	0.031111618946815568	0.0	6.264420696443212E-6
110-114	0.036666907220421416	0.0	6.264420696443212E-6
115-119	0.042794763545682166	0.0	6.264420696443212E-6
120-124	0.049524004257801464	0.0	6.264420696443212E-6
125-129	0.05757503773687027	0.0	6.264420696443212E-6
130-134	0.0661673171641118	0.0	6.264420696443212E-6
135-139	0.07545244152037991	0.0	6.264420696443212E-6
140-144	0.0854943078967784	0.0	6.264420696443212E-6
145-149	0.09567273864435932	0.0	6.264420696443212E-6
150-154	0.10631598940761633	6.264420696443212E-6	1.127595725359778E-5
155-159	0.1172411391022133	6.264420696443212E-6	1.2528841392886424E-5
160-164	0.12818633494303888	6.264420696443212E-6	1.2528841392886424E-5
165-169	0.1398343987860054	6.264420696443212E-6	1.2528841392886424E-5
170-174	0.15167290101814376	6.264420696443212E-6	1.2528841392886424E-5
175-179	0.16328337833693163	6.264420696443212E-6	1.2528841392886424E-5
180-184	0.17505171905726985	6.264420696443212E-6	1.2528841392886424E-5
185-189	0.18777475749174602	6.264420696443212E-6	4.2598060735813834E-5
190-194	0.20121444565389526	6.264420696443212E-6	5.0115365571545695E-5
195-199	0.21480573279689846	6.264420696443212E-6	5.0115365571545695E-5
200-204	0.22915752061244987	6.264420696443212E-6	5.0115365571545695E-5
205-209	0.2432837892829293	6.264420696443212E-6	5.0115365571545695E-5
210-214	0.2578961769994528	6.264420696443212E-6	5.0115365571545695E-5
215-219	0.27311871929180975	6.264420696443212E-6	5.0115365571545695E-5
220-224	0.288446503851867	6.264420696443212E-6	5.0115365571545695E-5
225-229	0.3039396691183104	6.264420696443212E-6	5.0115365571545695E-5
230-234	0.31845934340852644	6.264420696443212E-6	5.0115365571545695E-5
235-239	0.33282992448616716	6.264420696443212E-6	5.387401798941162E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTTGC	810660	0.0	244.83679	3
TTTGCTC	1003845	0.0	244.21289	5
TGTTTGA	145595	0.0	244.12494	3
CTGTTCG	124200	0.0	244.11232	2
CTGTTTG	959580	0.0	243.87787	2
GTTTGCT	1042540	0.0	243.54062	4
TTTGATC	144820	0.0	243.0714	5
CCTGTTC	125630	0.0	242.05524	1
CCTGTTT	971630	0.0	241.4522	1
GTTTGAT	150205	0.0	238.95674	4
TGTTCGC	122945	0.0	238.94199	3
CCCGTTT	230250	0.0	237.97719	1
CTCCCCA	795280	0.0	236.90707	9
GATCCCC	152155	0.0	236.90068	8
GCTCCCC	1240000	0.0	236.63245	8
TTGCTCC	1042955	0.0	236.19441	6
TGATCCC	149370	0.0	236.16739	7
CCTTTTG	13745	0.0	235.8203	2
TGCTCCC	1045995	0.0	235.44472	7
GTTCGCT	204465	0.0	234.62317	4
>>END_MODULE