FastQCFastQC Report
Fri 24 Apr 2015
000000000-AF4P8 l01n03.33300000008af9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AF4P8 l01n03.33300000008af9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15963168
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAATACCGGTCCAGGA2510431.5726389648971932No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGAACAGGCCCAGAG2261701.4168240289145615No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCATCAGCGTCAGTTATGGTCCAGTG1947251.2198393201149045No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCAGTTGTGGTCCAGTG1549810.9708661839554655No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGTGTCAGTATTGGGCCAGGA1399560.8767432629914063No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG1112820.6971172639415936No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCAGTAATGGTCCAGTG1093340.6849141724249221No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT1072060.671583485182891No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG991290.6209857592177191No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA886500.5553408947396907No Hit
CCTGTTTGCTCCCCACGCTGTCGCACCTGAGTGTCAGTATTGGGCCAGGT884840.5543010009040812No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG832500.5215130229788975No Hit
CCTGTTTGATCCCCACGCTGTCGCGCCTGAGCGTCAGTTACGAGCCAGTG785980.4923709378990436No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAATGGCCCAGAG780390.4888691267297319No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTACTGGTCCAGGA770890.4829179270681108No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG745750.46716917343725256No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCATCAGCGTCAGTTGTGGTCCAGTG732590.4589251958007333No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGGAAATGTCCAGAG729730.45713357148155054No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGTG722430.452560544373147No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTGACCCAGGG687300.430553634466542No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCCAACCAGGG678580.4250910596192435No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCATCAGCGTCAGTAATGGTCCAGTG663970.41593874098173994No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAATGCCGGTCCAGGA660120.4135269390136093No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGGCCCAGAA658910.41276894410933973No Hit
CCTGTTTGATCCCCACGCTATCGCGCCTGAGCGTCAGATACGAGCCAGTG640120.4009980976207229No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGGTCCAGTT639070.40034033344759634No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG637540.39938187708104056No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTCAAGGACCAGGT598300.3748002902681974No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAATGCCGGTCCAGGA584290.36602383687248047No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACTCCTTAGTA583550.3655602697409437No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCAGTTATGGTCCAGTG555880.34822661767388524No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA546480.34233806221922863No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGATCGTCAGTTCTGGTCCAGGA529720.3318388931319899No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG521840.32690252962319255No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATTGGTCCAGGA520910.3263199384984234No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA515490.32292462248095116No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG494270.30963152176309866No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAGTGTTGGTCCAGGA487210.30520884075140975No Hit
CCTGTTTGATCCCCACGCTGTCGCGCCTGAGCGTCAGAAATGAGCCAGTG441570.2766180246928429No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA433040.27127447383877684No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG414800.2598481704884644No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGTTGGTCCAGGT414260.2595098917708565No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAATGCCGGTCCAGGA410860.2573799887340658No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG395140.2475323193992571No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAACGTTCCAGAG394970.24742582424741758No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCAGCCCAGAG393110.24626064199787914No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG386360.24203215802777994No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGGCCCAGGA383110.2399962213014359No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGCG370990.23240374341734674No Hit
CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGAG368040.230555739311896No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG363790.22789336051590764No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG358620.22465465501584647No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGATGGTCCAGTT354170.22186698780592923No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT352470.2208020362875339No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTATCAGCGTCAGTATCCAACCAGGG351140.21996886833490695No Hit
CCTGTTTGCTCCCCATGCTTTCGCACCTCAGCGTCAGTATTAGGCCAGAA349720.21907932059601204No Hit
CCTGTTTGCTCCCCACGCTGTCGCACCTGAGTGTCAGTATTGGGCCAGGC333350.20882446391593448No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTGCTGGTCCAGGA329950.20669456087914378No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGAACAGGTCCAGAG328640.20587392176790975No Hit
CCTGTTTGATCCCCACGCTGTCGCGCCTGAGCGTCAGAAACGAGCCAGTG324940.20355608611022571No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTAACGTCCCAGCG324720.20341826885490402No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGAGTTGTCCAGGG321730.20154520706666748No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG312870.1959949303296188No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACTGTCCAGAA312150.19554389203947486No Hit
CCTGTTTGATCCCCACGCTGTCGCGCCTGAGCGTCAGTAATGAGCCAGTG305780.19155345605584054No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGCCCAGCA302560.18953631259158582No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGGACCAGGT301660.18897251472890594No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTAACAGCCCAGAG297760.1865293906572931No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCGGTATCTGTCCAGAA295470.1850948383178076No Hit
CCTGTTTGCTCCCCACGCTTTCGCACTTGAGTGTCAGTACTGGACCAGAG294910.18474403075880677No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA290810.18217561827326506No Hit
TACCGCGGCTGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC288980.18102922928581594Illumina Single End PCR Primer 1 (100% over 21bp)
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTACCGGCCCAGAG288590.18078491687865467No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAGTGTTGGTCCAGGA282540.1769949423573065No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG282370.176888447205467No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTATCAGCGTCAGTATCCAACCAGGT279840.17530354876926685No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA278860.1746896355410154No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGAG278720.1746019336512652No Hit
CCCTTTTGCTCCCCATGCTTTCGTGCCTGAGCGTCAGTAACTGTCCAGGT277820.17403813578858532No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAATACAGGTCTAGCC273340.17123167531657876No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG266980.16724750375364086No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGAACCAGGA265810.16651456653215702No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTACCGGGCCAGTG264050.16541202848958303No Hit
CCTGTTTGCTCCCCCGGCTTTCGTGCCTCAGTGTCAGGTGTTGTCCAGGG255870.16028773235989247No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG253450.1587717425513532No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCATCGTCCAGAA253090.15854622340628127No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGTGTCAATATTCGTGTAGCC246220.15424256638782477No Hit
CCTGTTTGATCCCCACGCTGTCGCGCCTGAGCGTCAGTGATGAGCCAGTG244390.15309617740037568No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCAGCCTAGCA242400.1518495576817835No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGTGTCAGCAACGGCCCAGCA239950.1503147746111549No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATTACGGGCCAGAT239010.14972591906568922No Hit
CCCTTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGCAATTGTCCAGGT236230.147984410112078No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACTTGTCCAGCG232470.14562898793021536No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG231330.14491484397082083No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTGCTGGACCAGGA228950.14342391184506734No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTTGGCCCAGCA227940.14279120535472659No Hit
CCTGTTTGATCCCCACGCTGTCGTGCATGAGCGTCAGTTACGAGCCAGTG226720.1420269460297605No Hit
TACCGCGGCGGCTGGCACACAATTACCATAGTGTAGATCTCGGTGGTCGC224340.14053601390400702Illumina Single End PCR Primer 1 (100% over 21bp)
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTAATGCCCAGAG224110.14039193222798882No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACAGCCCAGCA223470.13999100930341646No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTGAGCGTCGGTAACGGGCCAGTG221680.13886967799875313No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG220210.13794880815637597No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTTGATCCAGAT219610.13757294291458938No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTCCGGTCCAGTG218290.1367460393826589No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGGG215010.13469130939422552No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGA214150.13415256921433139No Hit
CCTGTTTGATCCCCACGCTATCGCGCCTGAGCGTCAGTAATGAGCCAGTG212160.1329059494957392No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGTG207200.12979879683030338No Hit
CCTGTTTGCTCCCCCAGCTTTCGTGCATCAGCGTCAGTTATGGTCCAGTG205400.1286712011049436No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTTATGGTCCAGTG202100.12660394227511731No Hit
CCTGTTTGATCCCCACGCTGTCGTGCATGAGCGTCAGAAACGGGCCAGTG200790.12578330316388328No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGTGTCAGTGTTGGCCCAGGA191410.11990727655061953No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAGGT190490.11933094984654675No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGATCCAGTG188300.11795904171402569No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAATATTGGTCCAGGA186970.11712587376139874No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAGTATTGGTCCAGGA186470.11681265272657658No Hit
CCCGTTTGCTCCCCTGGCTTTCGTTCCTCAGTGTCAGTTGCAGTCCAGAA186210.11664977778846905No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAATGCCGGTCCAGGA182490.11431941328939217No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGTGTTGTCCAGGG181780.11387463941994472No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGGTCCAGAT180180.1128723321085138No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATTGGCCCAGGA178170.11161318354852871No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGG178050.1115380105001714No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGTAATGGTCCAGTG176360.11047932340247249No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGCTTCAGCGTCAGTGTCAGCCCAGCA174540.10933919883571984No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA172570.10810510795852052No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGTCCAGAG172200.10787332439275212No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA171690.10755383893723351No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCAGTCCAGTG170900.10705894970221451No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACTCTTTAGTA168780.10573089251456853No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAATCCAGGTCTAGCC167640.10501674855517401No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG167260.10477870056870918No Hit
CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGGAACTGTCCAGAG164320.10293696088395488No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGGTAAGGCCCAGAG163450.10239195628336431No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG162170.10159011043421957No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTACAGGCCCAGAG162070.10152746622725516No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCCAACCAGGT160970.10083837995064639No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACATGTCCAGTG159900.10016808693612696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC8106600.0244.836793
TTTGCTC10038450.0244.212895
TGTTTGA1455950.0244.124943
CTGTTCG1242000.0244.112322
CTGTTTG9595800.0243.877872
GTTTGCT10425400.0243.540624
TTTGATC1448200.0243.07145
CCTGTTC1256300.0242.055241
CCTGTTT9716300.0241.45221
GTTTGAT1502050.0238.956744
TGTTCGC1229450.0238.941993
CCCGTTT2302500.0237.977191
CTCCCCA7952800.0236.907079
GATCCCC1521550.0236.900688
GCTCCCC12400000.0236.632458
TTGCTCC10429550.0236.194416
TGATCCC1493700.0236.167397
CCTTTTG137450.0235.82032
TGCTCCC10459950.0235.444727
GTTCGCT2044650.0234.623174
Produced by FastQC (version 0.11.2)