##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AF4P8 l01n02.33200000008afc.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15963168
Sequences flagged as poor quality	0
Sequence length	12
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.880032960875937	33.0	32.0	34.0	16.0	34.0
2	30.920516904915115	33.0	32.0	34.0	16.0	34.0
3	31.09391713474418	33.0	32.0	34.0	16.0	34.0
4	31.13597438804127	33.0	32.0	34.0	16.0	35.0
5	31.147035350376566	33.0	32.0	34.0	16.0	35.0
6	33.68820274271373	37.0	34.0	37.0	18.0	37.0
7	33.271642007401034	37.0	33.0	37.0	18.0	37.0
8	33.44854166791955	37.0	33.0	37.0	18.0	37.0
9	33.20618432381342	37.0	33.0	37.0	18.0	37.0
10	33.51526814727503	37.0	34.0	37.0	18.0	37.0
11	32.892427117223846	35.0	33.0	37.0	18.0	37.0
12	32.94163971712883	37.0	33.0	37.0	18.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.08259669739615205
1101	2	0.23712077970960266
1101	3	0.19139525898624754
1101	4	0.18816883925299166
1101	5	0.21143228491440524
1101	6	0.11009036940720307
1101	7	0.21142722808501446
1101	8	0.24865602260478425
1101	9	0.1915968441987843
1101	10	0.20384984622270963
1101	11	0.2164016983662691
1101	12	0.21602312024587889
1102	1	0.07728850639271556
1102	2	0.20830913130247097
1102	3	0.18216552646415707
1102	4	0.19250166273191383
1102	5	0.20461822045673372
1102	6	0.18454697447963753
1102	7	0.2312924911256502
1102	8	0.16738327468108594
1102	9	0.12216574709218975
1102	10	0.17346835863946808
1102	11	0.1896800279678459
1102	12	0.25077062443664744
1103	1	0.041190744731547824
1103	2	0.09747393689114148
1103	3	0.06579553728154153
1103	4	0.08099621280495839
1103	5	0.07044625640103064
1103	6	-0.002657926475393424
1103	7	0.07620136010019962
1103	8	0.032237651092067665
1103	9	0.03504426549381634
1103	10	0.06252807165786578
1103	11	0.10588514747845323
1103	12	0.021349465860438954
2112	1	0.05397314338161863
2112	2	0.1002987343691828
2112	3	0.05804620925551518
2112	4	0.03873702535389256
2112	5	0.07007575822949619
2112	6	0.026441733192733352
2112	7	0.08438690412126704
2112	8	0.10312816320735863
2112	9	0.09249630345431115
2112	10	0.05251206288963317
2112	11	0.09269913097322302
2112	12	0.03754277442310183
1113	1	0.06585391504412286
1113	2	-0.03107394381170181
1113	3	0.004280761010786449
1113	4	0.056736829833468505
1113	5	0.02336864036213626
1113	6	-0.09953976979194579
1113	7	0.014055047710236579
1113	8	0.09474243720895714
1113	9	0.013041430618201844
1113	10	-0.004692014058441885
1113	11	0.09105416973452662
1113	12	0.13583512443942425
2113	1	0.07738266237241476
2113	2	0.08442423091382878
2113	3	-0.011158566479977594
2113	4	0.06572090701208921
2113	5	0.082256317794279
2113	6	-0.14474450940183914
2113	7	0.06824200945480641
2113	8	0.11793746682380402
2113	9	0.025823388016554816
2113	10	0.051863629575493064
2113	11	0.059049585911360225
2113	12	0.18146805149128653
1112	1	0.141150041842117
1112	2	0.14093851752357622
1112	3	0.14753524478729574
1112	4	0.10498394839136083
1112	5	0.08621449547274906
1112	6	0.1303070387064622
1112	7	0.17530947939221164
1112	8	0.15655343240522512
1112	9	0.04835085023781005
1112	10	0.13209412103136486
1112	11	0.05912141013592276
1112	12	0.20935554710861481
2114	1	0.05946761483665597
2114	2	0.1062759769772974
2114	3	0.04746903496987542
2114	4	0.03707453743481537
2114	5	0.07725305577369213
2114	6	0.12877151005405807
2114	7	0.1362325236308095
2114	8	0.12228337579301751
2114	9	0.14279508946416541
2114	10	0.13557266468007612
2114	11	0.08418637494722248
2114	12	0.16150455430961586
1114	1	0.08046650347383277
1114	2	0.07424136065687748
1114	3	0.06451289356715151
1114	4	0.11865219933868687
1114	5	0.08776028388919244
1114	6	0.10064489502376972
1114	7	0.10743732089838431
1114	8	0.13322925813902486
1114	9	0.13239101140782594
1114	10	0.1344013699774962
1114	11	0.08741732770215549
1114	12	0.1544411701462849
1109	1	0.14589408125250003
1109	2	0.12150332465202851
1109	3	0.14454760581346804
1109	4	0.15611040750892613
1109	5	0.16164020644676924
1109	6	0.16363273360421715
1109	7	0.11909827063399092
1109	8	0.19350692614652587
1109	9	0.2086935678542332
1109	10	0.1995132391881569
1109	11	0.16246344593301387
1109	12	0.3196564887650837
1108	1	0.19026863021717233
1108	2	0.22435261532097428
1108	3	0.1843523993447782
1108	4	0.22863261821225223
1108	5	0.17945101612660963
1108	6	0.21622682699752716
1108	7	0.21995368164745344
1108	8	0.23347801198089257
1108	9	0.2386334609335421
1108	10	0.22483109189550277
1108	11	0.13077878806882381
1108	12	0.1884682102740527
1111	1	0.2164196385300201
1111	2	0.2165225352027491
1111	3	0.22289080828596752
1111	4	0.24757569559987758
1111	5	0.1049593728794278
1111	6	0.19609884273498324
1111	7	0.17679207363022442
1111	8	0.1922080772375523
1111	9	0.2149440333717081
1111	10	0.24672007736559465
1111	11	0.1613707914119047
1111	12	0.22788969309309692
1110	1	0.21457061633418917
1110	2	0.2257303230685892
1110	3	0.22235571662510267
1110	4	0.18138997211200092
1110	5	0.20520112256179246
1110	6	0.18412783628059515
1110	7	0.12102003332537947
1110	8	0.169430690996208
1110	9	0.1405832084756895
1110	10	0.20462236843913928
1110	11	0.05999581828544365
1110	12	0.22560780838994532
1105	1	0.2343454763862134
1105	2	0.2521140733310574
1105	3	0.2085896554828892
1105	4	0.17413065638519853
1105	5	0.1983424162562848
1105	6	0.17990233150309365
1105	7	0.13953690008741404
1105	8	0.28461761349040415
1105	9	0.17976505548846688
1105	10	0.24960848114625378
1105	11	0.24289841674617918
1105	12	0.30919481903220003
1104	1	-0.01159624622523836
1104	2	0.007224410292000272
1104	3	-0.02021460157384425
1104	4	-0.004817576891092301
1104	5	0.006748414770047617
1104	6	-0.028110665097877074
1104	7	0.03153760185438159
1104	8	0.06520152367404819
1104	9	0.005344444062728826
1104	10	-0.026098969361740387
1104	11	-0.02082463947558466
1104	12	0.10474111171472344
1107	1	-0.13945626172359127
1107	2	-0.10451121784348416
1107	3	-0.1349913871492454
1107	4	-0.07655514302359379
1107	5	-0.10924920170442576
1107	6	-0.10483547779223557
1107	7	-0.11341836473934563
1107	8	-0.16310949668433494
1107	9	-0.09724265577747815
1107	10	-0.08322091969089485
1107	11	-0.09413259858755652
1107	12	0.008579519497075694
1106	1	-0.1250303171296956
1106	2	-0.10195027794918943
1106	3	-0.15020861481271552
1106	4	-0.15912023102935535
1106	5	-0.12997964388639716
1106	6	-0.09275580338856315
1106	7	-0.1450452393299031
1106	8	-0.1286072538718912
1106	9	-0.1576065191923277
1106	10	-0.19778672650583218
1106	11	-0.08796104771248991
1106	12	-0.054946747404038376
2103	1	-0.1213064799827741
2103	2	-0.11035580655548927
2103	3	-0.1479445407024471
2103	4	-0.1062760780606169
2103	5	-0.10098381414195501
2103	6	-0.05147616205578487
2103	7	-0.028617512918884813
2103	8	-0.022549663812725385
2103	9	-0.11167630865300282
2103	10	-0.05670781443335926
2103	11	-0.0544229623511967
2103	12	-0.027567974905132075
2102	1	-0.2700014922099143
2102	2	-0.31906655710371723
2102	3	-0.2374079396471238
2102	4	-0.21911088556580083
2102	5	-0.25464596412664875
2102	6	-0.14552125885437306
2102	7	-0.17679521630894612
2102	8	-0.18064066959604475
2102	9	-0.23955370621791872
2102	10	-0.1918155784677822
2102	11	-0.17845839544018816
2102	12	-0.3210637404932015
2101	1	-0.22377025642068205
2101	2	-0.24860278314632112
2101	3	-0.27638310861012627
2101	4	-0.2618314578222858
2101	5	-0.23501606835560196
2101	6	-0.19840155992223174
2101	7	-0.22715887601228246
2101	8	-0.2850012816890839
2101	9	-0.2944674692013294
2101	10	-0.2866616352066558
2101	11	-0.2518049965134139
2101	12	-0.31928701351719013
2107	1	-0.22938187582929714
2107	2	-0.2677928718002711
2107	3	-0.21099233837512443
2107	4	-0.29496763792960223
2107	5	-0.22986653873185858
2107	6	-0.14842308988924913
2107	7	-0.2224777428739273
2107	8	-0.32621920142977956
2107	9	-0.3350249013518791
2107	10	-0.2886998568368
2107	11	-0.215263739678754
2107	12	-0.4956756806692795
2106	1	-0.20926742134280119
2106	2	-0.25676206492338594
2106	3	-0.17489560022650963
2106	4	-0.25171310718412343
2106	5	-0.2606073173596961
2106	6	-0.15438370769074794
2106	7	-0.24046524485085996
2106	8	-0.32293348704832425
2106	9	-0.2366117359684381
2106	10	-0.24157211682184254
2106	11	-0.12617360674853018
2106	12	-0.5689066293463583
2105	1	-0.08616660962944067
2105	2	-0.13558188185904996
2105	3	-0.13815525355563807
2105	4	-0.10297163586576374
2105	5	-0.13252925008139016
2105	6	-0.07099500090892263
2105	7	-0.1670790565860898
2105	8	-0.19193801439314484
2105	9	-0.12175961460280149
2105	10	-0.17029267617188992
2105	11	-0.03524906149456086
2105	12	-0.21888333456804787
2104	1	-0.16832641314000796
2104	2	-0.1693887587016114
2104	3	-0.07996256349355235
2104	4	-0.12811629432309246
2104	5	-0.17512851717593136
2104	6	-0.09642968228305904
2104	7	-0.19571497912209423
2104	8	-0.17486886851938976
2104	9	-0.17250060976009252
2104	10	-0.395185681394544
2104	11	-0.1680403579173344
2104	12	-0.28745005021637837
2111	1	-0.05546793701691044
2111	2	-0.1523409832758702
2111	3	-0.12822060675814484
2111	4	-0.051049721506711165
2111	5	-0.056594928922184096
2111	6	-0.06427807206090108
2111	7	-0.09105866137901586
2111	8	-0.18386016356966906
2111	9	-0.06554296924529979
2111	10	-0.032950652784045076
2111	11	-0.0839094515806238
2111	12	-0.16691712130192826
2110	1	0.04089055713869172
2110	2	-0.003077394600428107
2110	3	0.008539041274755022
2110	4	-0.06040904077370968
2110	5	0.0214554656081134
2110	6	-0.03322178305967327
2110	7	-0.161137937249606
2110	8	-0.29746218351510834
2110	9	-0.01029732084348467
2110	10	-0.06108292589557607
2110	11	-0.16007501575987249
2110	12	-0.12228337726664051
2109	1	-0.08273300752034274
2109	2	-0.14195844375088384
2109	3	-0.08315482292522347
2109	4	-0.11775000330671048
2109	5	-0.05540284170808718
2109	6	-0.10846125213524971
2109	7	-0.14446380131092695
2109	8	-0.04065824057866507
2109	9	0.029908113409355508
2109	10	-0.12128082166973542
2109	11	-0.2220099711530068
2109	12	-0.0830868710278807
2108	1	7.454888405789006E-4
2108	2	-0.05406696488996943
2108	3	0.04121425115997468
2108	4	-0.036722698689910516
2108	5	-0.05121924174856929
2108	6	-0.0765553711761271
2108	7	9.097069847072703E-4
2108	8	0.0032545992275103686
2108	9	0.020706997234633207
2108	10	0.08646300659047057
2108	11	-0.04467628924903977
2108	12	-0.08635954251128197
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	38.0
15	3618.0
16	152209.0
17	280093.0
18	281377.0
19	285115.0
20	230002.0
21	232479.0
22	218851.0
23	197256.0
24	202043.0
25	196955.0
26	188697.0
27	192742.0
28	202364.0
29	223173.0
30	260598.0
31	350748.0
32	582911.0
33	1373079.0
34	3747088.0
35	6032297.0
36	529435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.206628659173418	25.03977280700172	41.83013046031965	18.92346807350521
2	30.498922926760088	39.226771229042456	1.9597740222851767	28.314531821912283
3	15.887404054132615	21.23237066727607	46.48094288051093	16.39928239808038
4	13.803124793274115	12.984615585076847	31.36242129381837	41.84983832783067
5	12.128244218190273	57.047918057368065	17.597528260054645	13.226309464387018
6	32.47932991747002	20.675012629072125	22.202096726664784	24.64356072679308
7	47.424189233615785	20.990607879338235	25.338541823277183	6.246661063768796
8	24.374104461852713	9.598189381843842	27.680710224982295	38.346995931321146
9	14.830821801787716	32.79687340257272	40.16153936361504	12.21076543202452
10	24.050533077143584	18.410474662673472	31.570963858802966	25.96802840137998
11	33.96996761545077	13.832592628230186	16.295925721009766	35.90151403530928
12	10.960656431104402	30.87057030283713	29.581233499515886	28.58753976654258
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7300.0
1	7300.0
2	7300.0
3	7300.0
4	21838.0
5	36376.0
6	36376.0
7	36376.0
8	36376.0
9	36376.0
10	36376.0
11	36376.0
12	36376.0
13	67769.5
14	99163.0
15	99163.0
16	99163.0
17	99163.0
18	99163.0
19	99163.0
20	99163.0
21	147495.0
22	195827.0
23	195827.0
24	195827.0
25	195827.0
26	195827.0
27	195827.0
28	195827.0
29	310492.5
30	425158.0
31	425158.0
32	425158.0
33	425158.0
34	425158.0
35	425158.0
36	425158.0
37	425158.0
38	3558999.0
39	6692840.0
40	6692840.0
41	6692840.0
42	6692840.0
43	6692840.0
44	6692840.0
45	6692840.0
46	6121004.5
47	5549169.0
48	5549169.0
49	5549169.0
50	5549169.0
51	5549169.0
52	5549169.0
53	5549169.0
54	4168737.0
55	2788305.0
56	2788305.0
57	2788305.0
58	2788305.0
59	2788305.0
60	2788305.0
61	2788305.0
62	2788305.0
63	1453989.5
64	119674.0
65	119674.0
66	119674.0
67	119674.0
68	119674.0
69	119674.0
70	119674.0
71	75720.5
72	31767.0
73	31767.0
74	31767.0
75	31767.0
76	31767.0
77	31767.0
78	31767.0
79	21489.5
80	11212.0
81	11212.0
82	11212.0
83	11212.0
84	11212.0
85	11212.0
86	11212.0
87	11212.0
88	7157.5
89	3103.0
90	3103.0
91	3103.0
92	3103.0
93	3103.0
94	3103.0
95	3103.0
96	3188.5
97	3274.0
98	3274.0
99	3274.0
100	3274.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	6.264420696443212E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	6.264420696443212E-6
9	0.0
10	0.0
11	0.0
12	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
12	1.5963168E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.63684004271392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.10447799656343	3.5752113104701957
2	12.047700831457798	1.117265232758826
3	3.6050254757208426	0.501477794424786
4	1.898730963021733	0.3521644703871974
5	1.1696616942722897	0.2711767090215179
6	0.6268465434576755	0.17439522920048167
7	0.48318521059784797	0.15683167727830816
8	0.37867419942163866	0.1404681352816712
9	0.3219559796536022	0.13435725406041096
>10	1.9480913083678928	1.7473940586740524
>50	0.191319645874774	0.6202769923315886
>100	0.14073835013859734	1.3712973858150788
>500	0.04123202773912775	1.347985831282704
>1k	0.03936723726695234	3.3186575320431717
>5k	4.0807315171139714E-4	0.11699917401807247
>10k+	0.0025844632941680805	85.05404121295197
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATCAGTCTCGT	1093525	6.850300642078063	No Hit
CAACACGCACGA	854938	5.355691301375767	No Hit
AATCTTGCTGCA	809647	5.071969423613157	No Hit
CCTCTCGTGATC	808464	5.0645586139292655	No Hit
GGTCACTGACAG	796531	4.989805281758608	No Hit
AATGGAGCATGA	775243	4.856448293972726	No Hit
TATCAGGTGTGC	774971	4.854744371543292	No Hit
TGGCAAGACTCT	764969	4.7920876357374675	No Hit
TGTAATTGTCGC	719048	4.504419172936099	No Hit
AGCTTGACAGCT	715236	4.480539201241257	No Hit
TCGTCGATAATC	706276	4.4244099918011255	No Hit
TGCAATGTTGCT	696152	4.360988996670335	No Hit
TAATCCACAGCG	692967	4.341036816752164	No Hit
TCGGAGTGTTGT	677139	4.241883565968861	No Hit
GCCTGAATTTAC	661541	4.144171131945739	No Hit
CCATAGGGTTCA	638718	4.001198258390816	No Hit
TGTTATCGCACA	618453	3.8742497729773935	No Hit
GAAGAAGCGGTA	460962	2.887659893073856	No Hit
CCATGCGATAAC	220379	1.3805467686614588	No Hit
>>END_MODULE
>>Adapter Content	warn
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTCG	320	0.0	6.0000005	5
CGGCGAG	80	6.5394204E-5	6.0000005	2
GGAGCTT	80	6.5394204E-5	6.0000005	4
GAAGCCG	80	6.5394204E-5	6.0000005	4
TACCAGG	80	6.5394204E-5	6.0000005	1
GCAGTCG	395	0.0	6.0000005	2
CACGCCG	80	6.5394204E-5	6.0000005	4
CAACCCG	335	0.0	6.0000005	1
CACGACT	205	0.0	6.0000005	4
TCGGAGC	160	1.0913936E-11	6.0000005	1
TGCTGGA	80	6.5394204E-5	6.0000005	6
CAAGGCG	205	0.0	6.0000005	6
GACGGCT	80	6.5394204E-5	6.0000005	6
AGGTGCG	395	0.0	6.0000005	5
AAGCTCT	80	6.5394204E-5	6.0000005	5
TCACGGG	65	0.0012619761	6.0	3
TCGCCGG	75	1.751374E-4	6.0	1
GTGTTCC	55	0.0091607375	6.0	6
GTCATGC	55	0.0091607375	6.0	2
CTTCGGT	55	0.0091607375	6.0	2
>>END_MODULE