FastQCFastQC Report
Fri 24 Apr 2015
000000000-AF4P8 l01n02.33200000008afc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AF4P8 l01n02.33200000008afc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15963168
Sequences flagged as poor quality0
Sequence length12
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCAGTCTCGT10935256.850300642078063No Hit
CAACACGCACGA8549385.355691301375767No Hit
AATCTTGCTGCA8096475.071969423613157No Hit
CCTCTCGTGATC8084645.0645586139292655No Hit
GGTCACTGACAG7965314.989805281758608No Hit
AATGGAGCATGA7752434.856448293972726No Hit
TATCAGGTGTGC7749714.854744371543292No Hit
TGGCAAGACTCT7649694.7920876357374675No Hit
TGTAATTGTCGC7190484.504419172936099No Hit
AGCTTGACAGCT7152364.480539201241257No Hit
TCGTCGATAATC7062764.4244099918011255No Hit
TGCAATGTTGCT6961524.360988996670335No Hit
TAATCCACAGCG6929674.341036816752164No Hit
TCGGAGTGTTGT6771394.241883565968861No Hit
GCCTGAATTTAC6615414.144171131945739No Hit
CCATAGGGTTCA6387184.001198258390816No Hit
TGTTATCGCACA6184533.8742497729773935No Hit
GAAGAAGCGGTA4609622.887659893073856No Hit
CCATGCGATAAC2203791.3805467686614588No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTCG3200.06.00000055
CGGCGAG806.5394204E-56.00000052
GGAGCTT806.5394204E-56.00000054
GAAGCCG806.5394204E-56.00000054
TACCAGG806.5394204E-56.00000051
GCAGTCG3950.06.00000052
CACGCCG806.5394204E-56.00000054
CAACCCG3350.06.00000051
CACGACT2050.06.00000054
TCGGAGC1601.0913936E-116.00000051
TGCTGGA806.5394204E-56.00000056
CAAGGCG2050.06.00000056
GACGGCT806.5394204E-56.00000056
AGGTGCG3950.06.00000055
AAGCTCT806.5394204E-56.00000055
TCACGGG650.00126197616.03
TCGCCGG751.751374E-46.01
GTGTTCC550.00916073756.06
GTCATGC550.00916073756.02
CTTCGGT550.00916073756.02
Produced by FastQC (version 0.11.2)