##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AF4P8 l01n01.33100000008aff.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15963168
Sequences flagged as poor quality	0
Sequence length	251
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40995327493891	33.0	32.0	34.0	32.0	34.0
2	32.54413685303569	33.0	32.0	34.0	32.0	34.0
3	32.55587499924827	33.0	32.0	34.0	32.0	34.0
4	32.37125387642353	33.0	32.0	34.0	32.0	34.0
5	31.839964849082588	33.0	32.0	34.0	30.0	34.0
6	33.94520454837035	34.0	33.0	37.0	32.0	37.0
7	34.404596443512965	35.0	33.0	37.0	32.0	37.0
8	32.63673839678941	33.0	32.0	34.0	31.0	35.0
9	33.1027925033427	33.0	33.0	35.0	31.0	37.0
10-14	34.281377931999465	35.2	34.0	36.4	31.4	37.4
15-19	37.025959208097035	38.0	37.2	38.0	36.0	38.0
20-24	36.46907397078074	38.0	36.8	38.2	33.8	38.2
25-29	36.481852123588496	38.0	36.8	38.2	34.0	38.2
30-34	37.98126177711091	39.0	38.2	39.0	37.0	39.0
35-39	37.39442261085018	38.4	37.6	38.4	36.4	38.6
40-44	37.39894299176704	38.6	37.8	38.6	35.6	39.0
45-49	36.54936273301139	38.0	36.8	38.0	34.2	38.8
50-54	37.20347458599697	38.2	37.8	38.2	35.8	39.0
55-59	36.26047084137686	38.0	37.2	38.2	32.6	38.6
60-64	36.830962425503515	38.2	37.2	39.0	33.8	39.0
65-69	36.774923962461585	38.4	37.0	39.0	34.0	39.0
70-74	36.8952974246716	38.2	37.0	39.0	34.2	39.0
75-79	37.35864513860908	39.0	37.6	39.0	36.2	39.0
80-84	36.405328992340365	38.2	37.0	38.6	32.8	39.0
85-89	36.45840527394061	38.0	36.6	38.6	33.6	39.0
90-94	36.612931894220495	38.4	37.4	39.0	33.4	39.0
95-99	35.83974353962823	38.0	36.8	38.8	30.8	39.0
100-104	36.304892086583315	38.0	37.0	39.0	31.8	39.0
105-109	35.95299305250687	38.0	37.0	39.0	30.4	39.0
110-114	36.46038713618751	38.2	37.0	39.0	31.8	39.0
115-119	35.82810952061646	38.0	36.6	38.6	30.0	39.0
120-124	35.65178408195667	38.0	36.8	39.0	28.2	39.0
125-129	35.22696286852334	38.0	36.2	38.8	26.6	39.0
130-134	35.00805658375581	37.8	35.0	38.2	26.4	39.0
135-139	34.535503328662585	37.8	35.0	38.4	24.6	39.0
140-144	34.59334009389615	37.2	34.6	38.0	24.8	39.0
145-149	35.51627073022097	37.8	36.6	38.8	28.6	39.0
150-154	34.62271795924218	37.0	34.4	38.0	25.6	39.0
155-159	34.56064391479185	37.0	34.8	38.0	26.0	38.4
160-164	34.09263603565408	37.0	34.0	38.0	21.4	38.6
165-169	34.141319229366	37.0	34.4	38.0	24.8	38.4
170-174	34.060239496320534	37.0	34.8	38.0	20.8	38.2
175-179	33.106170717491665	37.0	31.6	37.8	18.6	38.0
180-184	32.594696240746195	36.2	31.2	37.2	20.4	38.0
185-189	33.799433032340445	37.0	34.0	37.0	21.6	38.0
190-194	32.990883288329734	37.0	32.0	37.0	22.0	38.0
195-199	31.491610324466922	34.4	29.4	37.0	15.4	37.0
200-204	30.953132937021017	34.4	27.6	37.0	12.4	37.0
205-209	33.11115220988716	37.0	32.8	37.0	18.2	37.0
210-214	32.04575205873922	37.0	31.4	37.0	13.6	37.0
215-219	32.08722076971188	37.0	30.0	37.0	14.0	37.0
220-224	31.983716703351114	36.2	32.4	37.0	13.8	37.0
225-229	30.89042446962909	36.0	26.0	37.0	13.6	37.0
230-234	29.66545787152024	34.0	24.4	36.6	12.8	37.0
235-239	28.60148732381943	32.4	22.2	36.2	13.0	37.0
240-244	29.495139248049007	32.8	23.2	37.0	12.6	37.0
245-249	28.405694834509042	32.4	22.2	37.0	13.0	37.0
250-251	25.788095884225488	28.0	18.5	34.5	12.5	37.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.12962129544799694
1101	2	0.1384521123901763
1101	3	0.0769813753675308
1101	4	0.1773498416672794
1101	5	0.34212840876808315
1101	6	0.1968191685811007
1101	7	0.2542842436664756
1101	8	0.26553294118771475
1101	9	0.26271078070383425
1101	10-14	-0.0735325668562794
1101	15-19	0.19918412487496084
1101	20-24	0.5529981156459911
1101	25-29	0.48972592471622534
1101	30-34	0.2380816492817388
1101	35-39	0.21547346018107305
1101	40-44	0.2773429653336237
1101	45-49	0.2786656339451383
1101	50-54	0.20078926781710038
1101	55-59	0.25604931558538624
1101	60-64	0.219063033022465
1101	65-69	0.25094489285952193
1101	70-74	0.2625496101895308
1101	75-79	0.16239898194122304
1101	80-84	0.2061258257736469
1101	85-89	0.326437344695087
1101	90-94	0.2532877909199982
1101	95-99	0.34112511685915337
1101	100-104	0.2351726528776794
1101	105-109	0.27386155245221744
1101	110-114	0.16052026713303036
1101	115-119	0.28882867455616434
1101	120-124	0.3814562437693496
1101	125-129	0.29087198573273554
1101	130-134	0.5171221825885297
1101	135-139	0.33762279861582556
1101	140-144	0.06914966363562058
1101	145-149	0.22666417783283066
1101	150-154	0.26206258869383703
1101	155-159	0.3395044294953564
1101	160-164	0.43102280499970647
1101	165-169	0.39189752101197683
1101	170-174	0.18857533065619236
1101	175-179	1.0251009331407843
1101	180-184	0.2554312195942572
1101	185-189	0.1382420525837773
1101	190-194	0.579415821843078
1101	195-199	0.650602492335377
1101	200-204	1.082239168288428
1101	205-209	0.34790105530011317
1101	210-214	0.5294621463223592
1101	215-219	0.4957170716482153
1101	220-224	0.4264630344852449
1101	225-229	0.5854822674746742
1101	230-234	0.5752367665675919
1101	235-239	-0.1666112573976264
1101	240-244	0.7154688838747063
1101	245-249	0.7129861678152061
1101	250-251	0.8508856895706458
1102	1	0.09489912117491173
1102	2	0.0914114129320609
1102	3	0.0712044024218983
1102	4	0.11697740754499364
1102	5	0.26310400074834916
1102	6	0.09959179899623649
1102	7	0.20000220249490042
1102	8	0.3055771225385371
1102	9	0.29381682237190176
1102	10-14	0.3990559713392301
1102	15-19	0.23509547569915412
1102	20-24	0.5328493437222406
1102	25-29	0.31850175390546553
1102	30-34	0.2289066314790702
1102	35-39	0.18841085458900864
1102	40-44	0.2541359973623756
1102	45-49	0.24515990781217312
1102	50-54	0.21036904134350465
1102	55-59	0.24188179217961192
1102	60-64	0.22320557405667074
1102	65-69	0.22746821971127673
1102	70-74	0.23202406129573205
1102	75-79	0.14884110434232412
1102	80-84	0.17701638389669938
1102	85-89	0.2815336835346116
1102	90-94	0.28407163977237104
1102	95-99	0.31514395887095503
1102	100-104	0.2264190566652573
1102	105-109	0.24141555376910162
1102	110-114	0.2553674755745732
1102	115-119	0.28630133639403255
1102	120-124	0.36302569945092955
1102	125-129	0.3343226946364837
1102	130-134	0.5721161422605263
1102	135-139	0.46155798770566037
1102	140-144	0.2031513680151562
1102	145-149	0.33298929307423464
1102	150-154	0.39059924223752773
1102	155-159	0.3917821942632358
1102	160-164	0.596449648051383
1102	165-169	0.6510819144400415
1102	170-174	0.41130405770900325
1102	175-179	1.132022358592046
1102	180-184	0.6139142080207236
1102	185-189	0.3388148419925372
1102	190-194	0.7997300941239658
1102	195-199	0.8438746756996132
1102	200-204	1.1420755666903482
1102	205-209	0.4629046875416094
1102	210-214	0.4954211695801831
1102	215-219	0.6449608602412695
1102	220-224	-0.6350691891009923
1102	225-229	0.3474507445302386
1102	230-234	0.8661693659951482
1102	235-239	-0.2838972518781482
1102	240-244	0.9445730702121757
1102	245-249	1.150080091115047
1102	250-251	0.7792790729645986
1103	1	0.12990743191851095
1103	2	0.059259513866791735
1103	3	0.0072044992335449365
1103	4	0.11535426895427747
1103	5	0.1873825843565058
1103	6	0.11690649893101579
1103	7	0.17485196250589752
1103	8	0.1147660319713566
1103	9	0.19129754529721765
1103	10-14	-0.19240605810104228
1103	15-19	0.12038176791966748
1103	20-24	0.4999923333698604
1103	25-29	0.3273250183790921
1103	30-34	0.21356177275653465
1103	35-39	0.1688103887795549
1103	40-44	0.2507370970374865
1103	45-49	0.24686341000514744
1103	50-54	0.21379959687946837
1103	55-59	0.197809779920064
1103	60-64	0.1805429939495511
1103	65-69	0.22765131209914102
1103	70-74	0.24679972053824883
1103	75-79	0.1333683323416892
1103	80-84	-0.04539046043851158
1103	85-89	0.21259041985948812
1103	90-94	0.02801840550901602
1103	95-99	0.32681211586411507
1103	100-104	0.2814992539008898
1103	105-109	0.22993016348384998
1103	110-114	0.18698003939822883
1103	115-119	0.38062260016139504
1103	120-124	0.43183630731710565
1103	125-129	0.361545014767529
1103	130-134	0.4754132806872633
1103	135-139	0.4970483401994201
1103	140-144	0.0958789044438646
1103	145-149	0.07520315284660484
1103	150-154	0.24734932783339048
1103	155-159	0.18772438245173362
1103	160-164	0.5665442689554112
1103	165-169	0.5782595941070241
1103	170-174	0.4117441391087979
1103	175-179	1.035011167191783
1103	180-184	0.7499751204230662
1103	185-189	0.28317915749885003
1103	190-194	0.5840159188614891
1103	195-199	0.8180603767973729
1103	200-204	1.1416124955978368
1103	205-209	0.482929279104205
1103	210-214	0.5714114048612302
1103	215-219	0.6059072034275204
1103	220-224	0.5726438198104162
1103	225-229	0.3905867290555953
1103	230-234	0.5291957186554548
1103	235-239	-0.35330058499325645
1103	240-244	0.7084853377039337
1103	245-249	0.9739212603457723
1103	250-251	0.7347250256341269
2112	1	0.14502906221866851
2112	2	0.044273889182598225
2112	3	-0.006618992154528769
2112	4	0.0771687131239247
2112	5	0.2022257964555081
2112	6	0.09960746596971148
2112	7	0.21263393427872757
2112	8	0.2711882572503299
2112	9	0.26997139976514717
2112	10-14	0.2955424484166116
2112	15-19	0.22021632841668293
2112	20-24	0.507528903893629
2112	25-29	0.2948815084538339
2112	30-34	0.23379233808118727
2112	35-39	0.17639143631406995
2112	40-44	0.2675881398392548
2112	45-49	0.22072363228008385
2112	50-54	0.17933334778805943
2112	55-59	0.25188348980547204
2112	60-64	0.19911137846459326
2112	65-69	0.23409610550972815
2112	70-74	0.28042224163046114
2112	75-79	0.17012397590178097
2112	80-84	0.22485707772166563
2112	85-89	0.2835057469857105
2112	90-94	0.08689104469311104
2112	95-99	0.1636919326907389
2112	100-104	0.10294129771569516
2112	105-109	-0.22574942169465828
2112	110-114	0.13430847855901362
2112	115-119	0.32498096719660907
2112	120-124	0.46453597473774977
2112	125-129	0.34296484707530084
2112	130-134	0.5559278120864661
2112	135-139	0.4065280045287878
2112	140-144	0.027051671546139744
2112	145-149	0.232583345540597
2112	150-154	0.3197984077131508
2112	155-159	0.2385239835438071
2112	160-164	0.5609039965769469
2112	165-169	0.6257420314135018
2112	170-174	0.3657469912810285
2112	175-179	0.99830070535603
2112	180-184	0.6665242485177245
2112	185-189	0.26153651100029407
2112	190-194	0.6589054787956528
2112	195-199	0.7565657633891583
2112	200-204	1.2054737628555579
2112	205-209	0.5145341976064444
2112	210-214	0.4903288468651681
2112	215-219	0.547987609664041
2112	220-224	0.40611132206869627
2112	225-229	0.5479581534530098
2112	230-234	0.871491834848662
2112	235-239	1.35417768820734
2112	240-244	0.9066431564470427
2112	245-249	1.2321506013826387
2112	250-251	0.37998576378778637
1113	1	0.13786438462702932
1113	2	0.03363577329482581
1113	3	-0.018202521319651055
1113	4	0.07515891841422473
1113	5	0.13327960625460733
1113	6	0.09264388622202802
1113	7	0.121982001552432
1113	8	0.09770414349659262
1113	9	0.13082304894250996
1113	10-14	-0.2610477137026024
1113	15-19	0.0802610494653635
1113	20-24	0.4195143303673845
1113	25-29	0.3593077680599279
1113	30-34	0.21722673379308333
1113	35-39	0.16849409370356483
1113	40-44	0.19333582615827538
1113	45-49	0.1838837010493748
1113	50-54	0.17411894763127833
1113	55-59	0.17873110463316522
1113	60-64	0.167272145006244
1113	65-69	0.24933138037843605
1113	70-74	0.23833156087555807
1113	75-79	0.14781083990696686
1113	80-84	0.21098219887790037
1113	85-89	0.33559138114982545
1113	90-94	0.0020371269074388465
1113	95-99	0.27731909259016163
1113	100-104	0.17204415251033112
1113	105-109	0.20972445647283422
1113	110-114	0.14368731381474475
1113	115-119	0.20150470897824846
1113	120-124	0.3280277092648234
1113	125-129	0.2522212792433436
1113	130-134	0.4445435251530441
1113	135-139	0.38571884781375587
1113	140-144	-0.14881969780979887
1113	145-149	0.1442998740932211
1113	150-154	0.20867924610759303
1113	155-159	0.0855435717001285
1113	160-164	0.45121483774944693
1113	165-169	0.40223701781835786
1113	170-174	0.31088902494091997
1113	175-179	1.0276355649822406
1113	180-184	0.4525124182159388
1113	185-189	0.1837463515553921
1113	190-194	0.5444157546716895
1113	195-199	0.5524422710583927
1113	200-204	0.9212003869175156
1113	205-209	0.38707701706587727
1113	210-214	0.3466637111270927
1113	215-219	0.5304564476619973
1113	220-224	0.3444646492492325
1113	225-229	0.41107694681938867
1113	230-234	0.2508149819641119
1113	235-239	1.0245170305523068
1113	240-244	0.5889864935561633
1113	245-249	0.8275767468017889
1113	250-251	0.07599994968549595
2113	1	0.13598977443665916
2113	2	0.024971345501079156
2113	3	-0.07658464993780001
2113	4	0.010362687071477694
2113	5	0.1332768882076465
2113	6	0.09160435833767622
2113	7	0.19338314047021044
2113	8	0.2656672500287627
2113	9	0.25083220080068
2113	10-14	0.2797895216556654
2113	15-19	0.16324426533727632
2113	20-24	0.4400299562183747
2113	25-29	0.3119969015144406
2113	30-34	0.2285937608914992
2113	35-39	0.18655867146973293
2113	40-44	0.19869730209789793
2113	45-49	0.14001914412792615
2113	50-54	0.1644929260423993
2113	55-59	0.13446302638091367
2113	60-64	0.12660053058544207
2113	65-69	0.20825351093691324
2113	70-74	0.16473766542686263
2113	75-79	0.14062971812522562
2113	80-84	0.23079201406955718
2113	85-89	0.35773627662282337
2113	90-94	0.17334724913839494
2113	95-99	0.31678868196300414
2113	100-104	0.17356460910235683
2113	105-109	0.18321460061046935
2113	110-114	0.1739660114791164
2113	115-119	0.22337595177377523
2113	120-124	0.37597200807369546
2113	125-129	0.24303563809139916
2113	130-134	0.5647577690992591
2113	135-139	0.2279225311838715
2113	140-144	0.12406172446431185
2113	145-149	0.1659686041071211
2113	150-154	0.23358469099292023
2113	155-159	0.308370248858445
2113	160-164	0.4428648078600048
2113	165-169	0.5310379111066723
2113	170-174	0.23450914700162429
2113	175-179	0.9644723378369022
2113	180-184	-0.05756065252953135
2113	185-189	-0.043281709794413814
2113	190-194	0.4350971946870317
2113	195-199	0.4933617573690441
2113	200-204	0.8806112298437796
2113	205-209	0.30307724331457564
2113	210-214	0.23101645720218045
2113	215-219	0.41090304700095004
2113	220-224	0.4188015025710925
2113	225-229	0.4704596153870071
2113	230-234	0.6771233747073389
2113	235-239	-0.3744037470550552
2113	240-244	0.9210082052994402
2113	245-249	0.32361048020670324
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2106	110-114	-0.13430672150605005
2106	115-119	-0.33606763581601484
2106	120-124	-0.1515037967052919
2106	125-129	-0.2396227863501892
2106	130-134	-3.5171517644875365
2106	135-139	-0.5702299845272449
2106	140-144	-1.495311744808724
2106	145-149	-0.4450409696630473
2106	150-154	-0.4181527995513221
2106	155-159	-0.3265865307017819
2106	160-164	-0.12734497132146316
2106	165-169	-0.4423204180521978
2106	170-174	-0.23660647714149974
2106	175-179	-4.966002107296298
2106	180-184	-0.2853805616880223
2106	185-189	-0.17858370872624363
2106	190-194	-0.5023403049301649
2106	195-199	-0.6671469866410149
2106	200-204	-0.02408170663767706
2106	205-209	-0.5599796273967783
2106	210-214	-0.44979253159056753
2106	215-219	-0.2943941166088351
2106	220-224	-0.34403814944075606
2106	225-229	-0.8339993591014832
2106	230-234	-0.5681929528353251
2106	235-239	0.021025637304184386
2106	240-244	-0.9100239406428443
2106	245-249	-0.22298195724730263
2106	250-251	-0.5420820463435128
2105	1	-0.2462641620920536
2105	2	-0.19052237927620297
2105	3	-0.18279312805327663
2105	4	-0.23940685193399247
2105	5	-0.3915412674584573
2105	6	-0.3202629481416821
2105	7	-0.3165758690238434
2105	8	-0.3798454135488569
2105	9	-0.5935404297364073
2105	10-14	-0.24418531453287784
2105	15-19	-0.22793331198528932
2105	20-24	-0.1755755424364125
2105	25-29	-0.5139845730164296
2105	30-34	-0.3760093180906878
2105	35-39	-0.2038206695083744
2105	40-44	-0.206908914476287
2105	45-49	-0.29641166172262245
2105	50-54	-0.2872253796691453
2105	55-59	-0.24145369692518415
2105	60-64	-0.22031987588677282
2105	65-69	-0.17197659566838297
2105	70-74	-0.32647058842594845
2105	75-79	-0.20502103232055902
2105	80-84	-0.2972110885668968
2105	85-89	-0.3230720601094177
2105	90-94	-0.2004291229081332
2105	95-99	-0.29389353598242707
2105	100-104	-0.15720753856057001
2105	105-109	-0.19021735312099253
2105	110-114	-0.23337460674645172
2105	115-119	-0.5676565436064962
2105	120-124	-0.23178254515334373
2105	125-129	-0.27191043492487665
2105	130-134	-0.5408044330560955
2105	135-139	-0.46570878408115135
2105	140-144	-1.4363236806073516
2105	145-149	-0.5182646354763065
2105	150-154	-0.5439670227777782
2105	155-159	-0.5029881912944418
2105	160-164	-0.22251492755702174
2105	165-169	-2.447634582363243
2105	170-174	-0.5638017579526107
2105	175-179	0.11502107724474087
2105	180-184	-0.21425647020319616
2105	185-189	-0.3440222000587241
2105	190-194	-0.5642393869158511
2105	195-199	-0.7859382744541037
2105	200-204	-0.5849912890844386
2105	205-209	-0.3965141379254149
2105	210-214	-0.5412034847003575
2105	215-219	-0.4343893818049054
2105	220-224	-0.43305508677104143
2105	225-229	-0.8977023968483131
2105	230-234	-0.9462822524304997
2105	235-239	-0.3618377145119034
2105	240-244	-1.0777384840219497
2105	245-249	-0.35054346452808716
2105	250-251	-0.5475880542891964
2104	1	-0.17368685646332693
2104	2	-0.11458912045100789
2104	3	-0.037011153686371756
2104	4	-0.11723752864949688
2104	5	-0.04569273577994437
2104	6	-0.04194938814787008
2104	7	-0.17099029727867787
2104	8	-0.18143243577033275
2104	9	-0.2197877819869518
2104	10-14	0.025737456844360906
2104	15-19	-0.21930482410301266
2104	20-24	-0.15905507232410798
2104	25-29	-0.26190135608313625
2104	30-34	-0.1896029587069279
2104	35-39	-0.15585988084767877
2104	40-44	-0.18005483847453974
2104	45-49	-0.2172482386763619
2104	50-54	-0.17567897449072944
2104	55-59	-0.22748789111546586
2104	60-64	-0.23256512352746483
2104	65-69	-0.2525242681833504
2104	70-74	-0.30950213434005036
2104	75-79	-0.2688616833318491
2104	80-84	-0.2683173831584327
2104	85-89	-0.40919125828455805
2104	90-94	-0.07207378058581071
2104	95-99	-0.09328914539612754
2104	100-104	-0.18599252326934135
2104	105-109	-0.14974713309155163
2104	110-114	-0.2276443014762819
2104	115-119	-0.49209081996107784
2104	120-124	-0.12202094298505983
2104	125-129	-0.23776925114814418
2104	130-134	-0.15065869775453677
2104	135-139	-0.40948344471539855
2104	140-144	0.13276166188671112
2104	145-149	-0.2546300548343652
2104	150-154	-0.43860868036856715
2104	155-159	-0.37158079801166366
2104	160-164	-0.11559094244116608
2104	165-169	-0.27692512871313824
2104	170-174	-0.28859858599663113
2104	175-179	0.2845792333714172
2104	180-184	0.018908129605542
2104	185-189	-0.1957266429036082
2104	190-194	-0.3845055961596131
2104	195-199	-0.14827935919915802
2104	200-204	-0.04739797573961013
2104	205-209	-0.4716889890929039
2104	210-214	-0.3484398611408359
2104	215-219	-0.3863888515207634
2104	220-224	-0.2572806717719338
2104	225-229	-0.39205440903673505
2104	230-234	-0.6699512237212666
2104	235-239	-0.4953747419123218
2104	240-244	-0.9498186170271623
2104	245-249	-7.775651169573283
2104	250-251	-4.238641096258206
2111	1	-0.1496342419257033
2111	2	-0.0670267001436855
2111	3	0.003202634383811187
2111	4	-0.10849379595394026
2111	5	-0.30764986594922306
2111	6	-0.19729499983407095
2111	7	-0.19643512480939052
2111	8	-0.1849561306240446
2111	9	-0.42980185638514
2111	10-14	-0.06694634680764722
2111	15-19	-0.14704947212018737
2111	20-24	0.22571606990554471
2111	25-29	-0.12492901317442318
2111	30-34	-0.10136190840383108
2111	35-39	-0.12197053592957019
2111	40-44	-0.149809797592809
2111	45-49	-0.15895632175136853
2111	50-54	-0.18738709171614687
2111	55-59	-0.21284386920667941
2111	60-64	-0.22432502606211102
2111	65-69	-0.32452722523012767
2111	70-74	-0.3115227820563504
2111	75-79	-0.13697083397804022
2111	80-84	-0.12003626530210454
2111	85-89	-0.17908425316309007
2111	90-94	-0.12023920807745014
2111	95-99	-0.36730487554065405
2111	100-104	-0.20158002810131137
2111	105-109	-0.18159334409621408
2111	110-114	-0.17939809897112724
2111	115-119	-0.32870562913906554
2111	120-124	0.006169217148887185
2111	125-129	-0.21644791062848867
2111	130-134	-0.3911131495327922
2111	135-139	-0.4722346927410257
2111	140-144	0.28928379201708765
2111	145-149	-0.17048455464833268
2111	150-154	-0.2520031617429339
2111	155-159	-0.418792369680105
2111	160-164	0.03683585347938134
2111	165-169	-0.20957250557145812
2111	170-174	-0.2967133457787341
2111	175-179	0.38523200221219867
2111	180-184	0.07744033625948532
2111	185-189	-0.09125392205394434
2111	190-194	-0.23372530795685265
2111	195-199	-0.01693169769888314
2111	200-204	-0.0723214183762515
2111	205-209	-0.37658320196255346
2111	210-214	-0.3670197914200024
2111	215-219	-0.2326812174088957
2111	220-224	-0.05741838771636765
2111	225-229	-0.435808616079445
2111	230-234	-0.9554353447407316
2111	235-239	0.09406929248431695
2111	240-244	-0.4393922738015057
2111	245-249	-7.422790357922494
2111	250-251	-4.062025056047464
2110	1	-0.1737434514725109
2110	2	-0.027065577376397698
2110	3	0.03810340589736683
2110	4	-0.03685997720970846
2110	5	-0.16801498266588055
2110	6	-0.04300377219966833
2110	7	-0.1218442201889971
2110	8	-0.10118242151175849
2110	9	-0.08999897850817717
2110	10-14	0.0764470036849545
2110	15-19	-0.1483817554443405
2110	20-24	-1.7373628544096746
2110	25-29	-0.5592304217216153
2110	30-34	-0.0018960178128537564
2110	35-39	-0.08662846992503859
2110	40-44	-0.10376729279675345
2110	45-49	-0.21592368683954533
2110	50-54	-0.12787220798927024
2110	55-59	-0.08240722168648773
2110	60-64	-0.11498663523210695
2110	65-69	-0.27342966840020466
2110	70-74	-0.15035016956220915
2110	75-79	-0.10440310412627696
2110	80-84	-0.16825476444225984
2110	85-89	-0.1250828019905157
2110	90-94	-0.002447395091003557
2110	95-99	-0.40662972503118766
2110	100-104	-0.33345093302841633
2110	105-109	-0.13713550467651459
2110	110-114	-0.15531515892763537
2110	115-119	-0.15909346528629698
2110	120-124	0.04245796250014422
2110	125-129	-0.16656660280442992
2110	130-134	-0.2377931546964689
2110	135-139	-0.21318251291589974
2110	140-144	0.5198612912347613
2110	145-149	-0.015266800989763851
2110	150-154	-0.109554689115825
2110	155-159	-0.1465406001558307
2110	160-164	0.26699870641113677
2110	165-169	0.013894301147821864
2110	170-174	-0.044472661502148014
2110	175-179	0.38421412919503695
2110	180-184	0.060353770621695446
2110	185-189	0.05420134517698472
2110	190-194	-0.01812119573028781
2110	195-199	0.11137491417205325
2110	200-204	-4.647739273729698
2110	205-209	-0.48500816540360603
2110	210-214	-0.04995952532467385
2110	215-219	-0.015471929659916839
2110	220-224	-0.06432397094030051
2110	225-229	-0.08695809238007968
2110	230-234	-0.2835196659812027
2110	235-239	0.46193560987303073
2110	240-244	-0.09830668276593357
2110	245-249	0.4476871883788611
2110	250-251	0.09586385290572963
2109	1	-0.15850885014688743
2109	2	-0.02633816199894312
2109	3	0.06922484755895653
2109	4	-0.004335313580313027
2109	5	-0.22368300728171775
2109	6	0.016668239809959573
2109	7	-0.09399727838511751
2109	8	-0.12921050867091566
2109	9	-0.06428662662763429
2109	10-14	0.13054401823256967
2109	15-19	-0.11861401305108643
2109	20-24	-1.7185577528476657
2109	25-29	-0.822479726348817
2109	30-34	-0.19709058119913436
2109	35-39	-0.07600858167005953
2109	40-44	-0.11401331479883936
2109	45-49	-0.1559042642818369
2109	50-54	-0.18540065100813052
2109	55-59	-0.26807330585904765
2109	60-64	-0.20718945529434052
2109	65-69	-0.2376862808885818
2109	70-74	-0.18570212802986674
2109	75-79	-0.11414045520940874
2109	80-84	-0.26752698132114006
2109	85-89	-0.3516444120034876
2109	90-94	-0.18519200593370755
2109	95-99	-0.2663429293186894
2109	100-104	-0.2970411552967249
2109	105-109	-0.3088632470706898
2109	110-114	-0.17323433220010287
2109	115-119	-0.2951982999421574
2109	120-124	-2.3988054294472363
2109	125-129	-0.6456333559043514
2109	130-134	-0.2810780673834046
2109	135-139	-0.440881840502243
2109	140-144	0.3489133743544599
2109	145-149	-0.24302279102276003
2109	150-154	-0.24099036417438668
2109	155-159	-0.27590660748235507
2109	160-164	0.03979152784697959
2109	165-169	-0.09914452542908947
2109	170-174	-0.08339245700946663
2109	175-179	0.3264236126229534
2109	180-184	-2.6468444992424445
2109	185-189	-0.2026959365539014
2109	190-194	-0.2521937805939274
2109	195-199	-0.049973067554951456
2109	200-204	-4.1798713153192395
2109	205-209	-0.6703962130071162
2109	210-214	-0.20764844860256915
2109	215-219	-0.16612005603726132
2109	220-224	0.1718238427788279
2109	225-229	-0.13584943296221397
2109	230-234	-0.29570290178856595
2109	235-239	0.4090581684951857
2109	240-244	-0.31026095278397037
2109	245-249	0.23258229059783275
2109	250-251	-0.5532100602416783
2108	1	-0.20351481079775624
2108	2	-0.0971965191647115
2108	3	-0.03200120300047615
2108	4	-0.10112870888885084
2108	5	-0.18143945941993778
2108	6	0.01476651415259056
2108	7	-0.08391236613189079
2108	8	-0.09286677421000178
2108	9	-0.09760537308751793
2108	10-14	0.17015868748060115
2108	15-19	-0.02106334916820174
2108	20-24	-4.011440874236378
2108	25-29	-0.9129327096058688
2108	30-34	-0.11248961227121868
2108	35-39	-0.08786562384343455
2108	40-44	-0.10549477538950924
2108	45-49	-0.14916708131080725
2108	50-54	-0.12169675407253777
2108	55-59	-0.1663109737829842
2108	60-64	-0.15316531651184562
2108	65-69	-0.2772181138209504
2108	70-74	-0.1516758432193086
2108	75-79	-0.14471862809124048
2108	80-84	-0.2122013538864138
2108	85-89	-0.24696024703143848
2108	90-94	-0.05456086546919181
2108	95-99	-0.1593578793481072
2108	100-104	-0.14865624223042317
2108	105-109	-0.15896437006758646
2108	110-114	-0.15347145367576331
2108	115-119	-0.11738421847303471
2108	120-124	-0.081961312887465
2108	125-129	-0.14800673546725562
2108	130-134	-0.11603464203658831
2108	135-139	-0.19719097761496585
2108	140-144	0.5247947408614237
2108	145-149	-0.03404418395223985
2108	150-154	-0.07561013331245192
2108	155-159	-0.26995224714855226
2108	160-164	0.10329593688509675
2108	165-169	0.018707755564868478
2108	170-174	-0.03246088946659853
2108	175-179	0.48461707013101574
2108	180-184	-3.494113437990592
2108	185-189	-0.3657370898385608
2108	190-194	-0.20577487604492717
2108	195-199	0.06888474781821685
2108	200-204	-4.204893895685782
2108	205-209	-0.37773191291236685
2108	210-214	-0.09652538120491982
2108	215-219	0.02150424807653195
2108	220-224	-0.03790629030176973
2108	225-229	-0.11355049187624289
2108	230-234	-0.5572776632271363
2108	235-239	0.22976561702255438
2108	240-244	-0.38306441953126225
2108	245-249	0.6760420142863239
2108	250-251	-0.4360556679027141
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	20.0
14	70.0
15	225.0
16	332.0
17	490.0
18	1369.0
19	4757.0
20	14005.0
21	37200.0
22	86886.0
23	118712.0
24	132840.0
25	163660.0
26	214719.0
27	296903.0
28	387834.0
29	479924.0
30	611757.0
31	753612.0
32	897875.0
33	1077049.0
34	1351812.0
35	1939051.0
36	3522623.0
37	3775290.0
38	94152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	6.84787631126854	3.6509169107284967	86.36384707596888	3.1373597020340824
2	1.5299281445888435	93.46902820292313	2.833510240573801	2.1675334119142264
3	1.4506331074132655	3.018893242243645	2.472685872879368	93.05778777746372
4	70.93885123554422	24.420697696096415	2.2899026057985483	2.350548462560815
5	44.2190171775427	25.926282301858876	27.906465684004576	1.9482348365938391
6	17.368212876040644	78.10388263783229	2.57235280615978	1.9555516799672847
7	89.92317815611538	3.8007430605253294	3.1501015337306484	3.1259772496286455
8	90.10526607249889	4.13161096844937	2.594547648687278	3.168575310364459
9	75.93902413355545	6.086993509057851	12.837783828372915	5.1361985290137895
10-14	31.64096750720158	31.754237003582247	13.015875044352098	23.58892044486408
15-19	37.58495807348516	3.340995972729223	38.96964437134283	20.104401582442783
20-24	32.52056609314643	11.930575434650565	29.505214754364545	26.043643717838467
25-29	20.818304988082566	33.61031720019485	39.130378130456315	6.440999681266275
30-34	38.32389911576449	20.69884749693795	25.933223279990536	15.044030107307021
35-39	36.27431096383876	20.72700857373674	24.26842842222797	18.730252040196532
40-44	47.741601165883864	40.32650160669862	2.6648156556392815	9.267081571778233
45-49	33.85480250536735	5.194091799322039	28.42603924233586	32.52506645297475
50-54	55.92414864016967	21.001650800141928	6.157701278342745	16.916499281345658
55-59	33.55112218326588	9.35698227319289	34.371464360958925	22.720431182582303
60-64	29.10804171202107	36.74224815525339	21.345938350081887	12.803771782643647
65-69	28.949912699033177	18.530047419158905	30.27718808697622	22.242851794831704
70-74	41.96102553077184	21.542444457140338	31.00459131921684	5.491938692870988
75-79	8.18411107369164	40.97818302732891	19.561490551248976	31.276215347730474
80-84	50.04494220696042	10.667168321476037	11.140529248329655	28.147360223233886
85-89	2.453310019665269	38.30946087894333	27.989878951345997	31.247350150045406
90-94	30.08676047478196	8.700513800281092	18.349211593819263	42.86351413111768
95-99	28.36487379403479	34.2013143531574	23.161682232019135	14.272129620788675
100-104	28.398722609446946	14.056362747043693	25.174946476789568	32.36996816671979
105-109	25.66992842523489	45.15234820556922	19.91381660582661	9.263906763369276
110-114	27.846810858596488	11.94262943295466	26.319852049417758	33.89070765903109
115-119	32.58417752666639	15.300850056830825	29.039853492740285	23.075118923762503
120-124	41.741263911289586	29.137390888041658	24.694778939804166	4.426566260864589
125-129	45.08348969327392	17.84293694083781	18.509455015445557	18.56411835044272
130-134	59.22665350637166	34.4065350937859	3.7470732626506216	2.6197381371918156
135-139	37.193544218629896	24.964571567863512	24.650021215087662	13.191862998418936
140-144	37.42430700472488	39.42077537491305	19.441655941978432	3.713261678383639
145-149	18.904961958741044	11.993268654358191	28.715051156700866	40.3867182301999
150-154	39.42688793169095	20.972783622217936	37.35651563289983	2.243812813191287
155-159	54.710627732806614	9.196619598230617	20.018648429150115	16.074104239812655
160-164	21.317581104642414	55.55459932651462	20.54591596219287	2.5819036066500987
165-169	33.38258003380231	20.020604181340552	24.758586134245686	21.83822965061145
170-174	22.907206999533187	39.36492954399514	35.031949862281095	2.6959135941905865
175-179	40.54678670984089	26.851845784644325	13.256933877497673	19.344433628017114
180-184	33.86610687467818	44.240783389381946	3.7450324082295547	18.148077327710322
185-189	23.725454088447957	30.77212820843903	7.11706992288924	38.385347780223775
190-194	54.62620452281152	4.81003018949622	20.35151418565538	20.212251102036888
195-199	36.48681184695684	40.980676780206494	2.767678731034826	19.764832641801835
200-204	35.19025804365116	14.092358638048236	20.909513233570426	29.807870084730176
205-209	37.217711567693016	9.982919805752335	25.6022535978668	27.19711502868785
210-214	23.067264207227446	26.924631511412954	16.824526941940107	33.18357733941949
215-219	20.862590737790317	21.90800609929095	33.81201612436931	23.417387038549432
220-224	18.356061341560572	23.751009464450018	19.937944145094914	37.9549850488945
225-229	46.66454169158757	24.830556812546405	9.768147770371089	18.736753725494932
230-234	30.73158988821241	42.648604403552085	3.2425582000404485	23.377247508195055
235-239	32.808158541063506	26.72636442498587	17.622879338523273	22.842597695427344
240-244	73.38464269748962	19.57717039625217	4.298284651267218	2.7399022549909895
245-249	21.15896748661547	52.64116944610879	4.401860748512855	21.79800231876288
250-251	11.989857651680271	39.07696386312315	2.6667483964804726	46.266430088716106
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.5
1	0.0
2	0.3333333333333333
3	1.0
4	2.333333333333333
5	0.3333333333333333
6	0.3333333333333333
7	0.0
8	0.0
9	0.0
10	0.6666666666666666
11	0.3333333333333333
12	0.0
13	0.6666666666666666
14	1.9999999999999998
15	5.666666666666665
16	10.666666666666668
17	22.999999999999986
18	34.666666666666636
19	44.33333333333337
20	56.00000000000012
21	60.66666666666682
22	66.6666666666668
23	69.0000000000001
24	72.33333333333339
25	108.99999999999953
26	163.66666666666694
27	291.0000000000009
28	496.33333333332257
29	757.0000000000161
30	1147.0000000000184
31	1747.6666666665485
32	2961.6666666668957
33	4112.666666667396
34	5295.66666666632
35	8159.666666663716
36	13625.66666667357
37	11994.333333337268
38	10192.33333333399
39	17970.666666672812
40	35828.33333330791
41	46248.666666717065
42	61437.66666682758
43	81055.99999996489
44	111321.99999952446
45	132094.66666593347
46	163419.00000017846
47	182940.33333407994
48	168012.00000031214
49	189588.33333427343
50	239086.00000238066
51	334822.6666654879
52	636003.666667465
53	912878.0000330308
54	1000216.666709865
55	944514.333370047
56	805715.6666872221
57	608517.9999975986
58	442767.3333258714
59	283351.0000018173
60	160787.66666676855
61	153302.33333321736
62	131197.9999992407
63	42053.00000001987
64	18597.66666667053
65	9913.333333333483
66	6094.333333332261
67	4453.333333333753
68	3091.000000000288
69	2504.3333333333544
70	2064.3333333331543
71	1591.666666666584
72	1052.6666666667065
73	612.6666666666664
74	329.33333333333206
75	179.0000000000007
76	121.33333333333269
77	121.66666666666602
78	109.99999999999952
79	71.00000000000007
80	56.00000000000012
81	27.666666666666636
82	20.33333333333333
83	15.666666666666677
84	12.666666666666671
85	10.333333333333334
86	8.666666666666664
87	8.999999999999998
88	11.333333333333336
89	13.666666666666673
90	12.000000000000004
91	7.33333333333333
92	7.9999999999999964
93	8.666666666666664
94	6.666666666666664
95	6.666666666666664
96	4.666666666666666
97	4.666666666666666
98	6.666666666666664
99	21.666666666666657
100	107.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	2.5057682785772853E-6
95-99	1.2528841392886426E-6
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	1.2528841392886426E-6
125-129	0.0
130-134	0.0
135-139	2.5057682785772853E-6
140-144	0.0
145-149	1.2528841392886426E-6
150-154	2.5057682785772853E-6
155-159	2.5057682785772853E-6
160-164	2.5057682785772853E-6
165-169	2.5057682785772853E-6
170-174	3.758652417865927E-6
175-179	1.2528841392886426E-6
180-184	2.5057682785772853E-6
185-189	2.5057682785772853E-6
190-194	0.0
195-199	1.2528841392886426E-6
200-204	3.758652417865927E-6
205-209	3.758652417865927E-6
210-214	3.758652417865927E-6
215-219	6.264420696443212E-6
220-224	3.758652417865927E-6
225-229	2.5057682785772853E-6
230-234	2.5057682785772853E-6
235-239	1.2528841392886426E-6
240-244	0.0
245-249	3.758652417865927E-6
250-251	6.264420696443212E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
251	1.5963168E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	3.8381668704785614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.67775508945881	2.8278751867559406
2	10.563140388737684	0.8108619097653402
3	3.445065808222319	0.3966821235521206
4	2.3628204072863674	0.36275596032548785
5	1.284770257896334	0.24655813200169532
6	0.887552743785141	0.20439453221990853
7	0.6232167899278244	0.1674407025358881
8	0.5026623473903198	0.1543441575032409
9	0.3625226810560422	0.12522802898037305
>10	3.7010491796547482	2.947335423588959
>50	1.1655999766601668	3.36854428710234
>100	1.1483514252331284	7.655924644288397
>500	0.10078912099404111	2.7317111195327106
>1k	0.12402728001464944	10.280365482335492
>5k	0.021301316919799175	5.804422207326327
>10k+	0.02937518676276814	61.915556102185676
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACGAGGGGGGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGGGTGCGTA	501830	3.1436742380960974	No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGCA	392402	2.4581712101257094	No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA	370264	2.3194894647478494	No Hit
TACAGAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA	325722	2.040459638086876	No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA	262119	1.6420236885309982	No Hit
TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCTCGTA	255363	1.599701262305828	No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA	240531	1.5067873745361824	No Hit
TACGGGGGGAGCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA	218976	1.3717577864243489	No Hit
TACGAGGGGGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGGGTGCGTA	215534	1.3501956503871915	No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA	207744	1.3013958131618988	No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA	173124	1.0845215686510348	No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA	156597	0.9809894878009177	No Hit
TACGTAGGGTGCAGGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA	154873	0.9701896265202496	No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGTGTGCGCA	145614	0.9121873552918819	No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA	143288	0.897616312751955	No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA	142713	0.8940142708515002	No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATGACTGGGCGTAAAGGGCGCGTA	139877	0.8762483737563873	No Hit
TACGTAGGGCGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA	138664	0.8686496314516016	No Hit
TACAGAGGGTGCAAGCGTTGTTCGGAATCATTGGGCGTAAAGGGCGTGTA	138352	0.8666951321943114	No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA	131131	0.8214597503452948	No Hit
TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA	122547	0.7676859630870263	No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA	118926	0.7450024957452055	No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA	116400	0.72917856906599	No Hit
TACGTAGGGACCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA	115065	0.7208155674362382	No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA	109901	0.6884660989598055	No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA	106903	0.6696853657118688	No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA	100884	0.631979817539977	No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA	92916	0.5820649134307175	No Hit
TACAGAGGTCCCGAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA	91223	0.5714592491916392	No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA	89028	0.5577088457629463	No Hit
TACGGAGGGTGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA	88394	0.5537372030414013	No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA	87650	0.5490764740432476	No Hit
TACAGAGACTGCAAGCGTTATTCGGATTCACTGGGCGTAAAGGGTGCGCA	78306	0.4905417270556822	No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA	76303	0.47799409240070645	No Hit
TACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA	73659	0.46143096407931056	No Hit
TACGTAGGGAGCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA	71965	0.4508190354195358	No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA	70754	0.4432328219561431	No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTGCGCA	70696	0.44286948555574934	No Hit
TACGGGGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCTCGTA	67765	0.4245084684944743	No Hit
TACGAAGGTGGCAAGCGTTGTTCGGATTTACTGGGCGTACAGGGAGCGTA	66763	0.4182315189566381	No Hit
TACAGAGGGTGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA	66422	0.41609535149915106	No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA	65141	0.4080706285870073	No Hit
TACGAGGGGGGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGGGTGCGTA	61506	0.3852994593554362	No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGCACGCA	60129	0.3766733520564339	No Hit
TACAGAGGTCTCAAGCGTTGTTCGGATTCATTGGGCGTAAAGGGTGCGTA	58647	0.3673894805843051	No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGCGCACGTA	57134	0.3579114120705865	No Hit
TACGGAGGGTGCGAGCGTTATCCGGAATTACTGGGTTTAAAGGGTGCGTA	52590	0.3294458844259485	No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA	48911	0.30639908068373395	No Hit
TACAGAGGGGGCAAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA	46860	0.2935507538353289	No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA	45020	0.28202421975387343	No Hit
TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA	44622	0.27953098031668905	No Hit
TACGTAGGGTGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGCTCGTA	42060	0.2634815344924015	No Hit
TACGTAGGTGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGGGCGCGTA	41217	0.2582006278452999	No Hit
TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGTGCGTA	40512	0.2537842112543074	No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA	39768	0.2491234822561537	No Hit
TACGTAGGGTGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGCTCGTA	39288	0.2461165603218609	No Hit
TACGAAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA	38366	0.24034076443974028	No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCGTGTA	35449	0.22206744926821542	No Hit
TACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA	35008	0.219304839741084	No Hit
TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGTTCGTA	34887	0.21854684483681433	No Hit
TACAGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA	34634	0.21696194640061423	No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCACGTA	33826	0.2119002944778881	No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA	33439	0.20947596366836457	No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA	32769	0.20527880180174762	No Hit
TACGAAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA	32767	0.20526627296035474	No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA	32659	0.20458971552513885	No Hit
TACGGAGGGGACTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA	32500	0.2035936726344044	No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA	31194	0.19541233920484954	No Hit
TACGGGGGGGGTAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCTCGTA	31091	0.1947671038731159	No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGAGCGTGTA	30861	0.19332628711293398	No Hit
CACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA	30756	0.19266852293980744	No Hit
TACCAGCACCCCGAGTGGTCGGGACGATTATTGGGCCTAAAGCATCCGTA	30572	0.1915158695316619	No Hit
GACGTAGGGGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA	30543	0.19133420133146503	No Hit
TACGTAGGCAGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGAGTGTGTA	30506	0.19110241776569664	No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA	30190	0.18912286082562058	No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA	30138	0.18879711094940554	No Hit
TACGTAGGGTGCGGGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA	30119	0.1886780869561731	No Hit
TACGTAGGGCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCTTGTA	29437	0.18440575204119883	No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA	29329	0.18372919460598297	No Hit
TACGAGGGGGGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGGGCGCGTA	28500	0.17853598984863156	No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA	27310	0.17108132921986413	No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA	26942	0.16877602240357303	No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCACTGGGTTTAAAGGGTGCGTA	26646	0.16692175387742583	No Hit
CACGGGGGGAGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGTGTA	26612	0.16670876357374678	No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCTTAAAGGGCATCTA	26543	0.16627651854569217	No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCGTGTA	26503	0.16602594171783444	No Hit
TACGAGGGGGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGGGCGCGTA	25706	0.16103319842276922	No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA	25674	0.16083273696048303	No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA	25425	0.15927289620706866	No Hit
GACAGAGGGGGCAAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA	24569	0.15391055209091328	No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA	24530	0.15366623968375198	No Hit
TACGTAGGGCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCTCGTA	24032	0.15054655817692328	No Hit
TACGAAGGGTGCAAACGTTGCTCGGAATTATTGGGCGTAAAGCGCACGTA	23771	0.1489115443751516	No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCGTGTA	23659	0.14820992925714996	No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATGACTGGGCGTAAAGGGCGCGTA	23617	0.14794682358789935	No Hit
GACGAACCGTGCGAACGTTGTTCGGAATCACTGGGCTTAAAGGGCGCGTA	23268	0.14576054076484066	No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGCGCGTA	23152	0.14503386796405324	No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA	22936	0.14368075309362152	No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA	22805	0.14286011398238746	No Hit
TACGGAGGGTGCAAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA	21734	0.1361509194164968	No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGCA	20994	0.1315152481011288	No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA	20983	0.13144633947346793	No Hit
TACAGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGCA	20936	0.1311519117007351	No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCGCGCA	20930	0.13111432517655644	No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA	20490	0.12835798007012142	No Hit
GACAGAGGGTGCAAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA	20027	0.12545755328766822	No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGAGCTCGTA	19857	0.12439260176927286	No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGTGCGTA	19853	0.12436754408648709	No Hit
TACAGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA	19762	0.12379748180311077	No Hit
TACGGAAGGGGCAAGCGTTGTTCGGAATCATTGGGCGTAAAGGGCGCGTA	19706	0.12344667424410993	No Hit
TACGTAGGGAGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCGCGTA	19587	0.1227012081812332	No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA	18631	0.1167124219954335	No Hit
GACGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA	18394	0.11522775429037645	No Hit
GACAGAGGGGGCAAGCGTTGCTCGGAATTACTGGGCGTAAAGGGCGCGTA	18286	0.11455119685516058	No Hit
TACGTAGGGTCCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA	17948	0.11243382265976277	No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA	17919	0.11225215445956592	No Hit
TACAGAGACCTCAAGCGTTATCCGGAATCATTGGGCGTAAAGCGTACCGA	17883	0.11202663531449397	No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA	17726	0.11104312126515238	No Hit
TACGAAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGCA	17326	0.1085373529865751	No Hit
TACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGTGTA	17308	0.10842459341403911	No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA	17247	0.10804246375155609	No Hit
CACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA	17127	0.10729073326798289	No Hit
TACAGAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA	16907	0.10591256071476539	No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGAGCGTA	16878	0.10573089251456853	No Hit
TACGGGGGGAGCAAACGTTGTTCGGAATCACTGGGCGTAAAGGGCGTGTA	16579	0.103857830726332	No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGTGTA	16454	0.10307477813927661	No Hit
TACGAAGGGAGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA	16399	0.10273023500097223	No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA	16356	0.10246086491102518	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	5.074180764119002E-4	0.0	0.0
2	5.074180764119002E-4	0.0	0.0
3	5.136824971083434E-4	0.0	0.0
4	5.136824971083434E-4	0.0	0.0
5	5.199469178047866E-4	0.0	0.0
6	5.262113385012298E-4	0.0	0.0
7	5.387401798941162E-4	0.0	0.0
8	5.387401798941162E-4	0.0	0.0
9	5.512690212870027E-4	0.0	0.0
10-14	5.901084296049506E-4	0.0	0.0
15-19	6.314536062014758E-4	0.0	0.0
20-24	6.953506973051965E-4	0.0	0.0
25-29	7.592477884089173E-4	0.0	0.0
30-34	8.507083305769882E-4	0.0	0.0
35-39	0.0010010544272916252	0.0	0.0
40-44	0.0012666658648208175	0.0	0.0
45-49	0.0017540377950040996	0.0	0.0
50-54	0.002431848114359255	0.0	0.0
55-59	0.00331387854841846	0.0	0.0
60-64	0.004502865596603381	0.0	0.0
65-69	0.005864750656010135	0.0	0.0
70-74	0.007503523110199679	5.01153655715457E-6	0.0
75-79	0.0094868387026936	6.264420696443212E-6	0.0
80-84	0.011919939701192143	6.264420696443212E-6	3.7586524178659275E-6
85-89	0.014682549228323603	6.264420696443212E-6	6.264420696443212E-6
90-94	0.01798389893534917	6.264420696443212E-6	6.264420696443212E-6
95-99	0.021925472437551243	6.264420696443212E-6	6.264420696443212E-6
100-104	0.026567408173615664	6.264420696443212E-6	6.264420696443212E-6
105-109	0.0317155091019527	6.264420696443212E-6	6.264420696443212E-6
110-114	0.03768550202566308	6.264420696443212E-6	6.264420696443212E-6
115-119	0.044146625531974605	6.264420696443212E-6	6.264420696443212E-6
120-124	0.05520332806119688	6.264420696443212E-6	6.264420696443212E-6
125-129	0.06609590276817233	6.264420696443212E-6	6.264420696443212E-6
130-134	0.07475333217065686	6.264420696443212E-6	6.264420696443212E-6
135-139	0.08395075463717477	0.0040418042333451604	6.264420696443212E-6
140-144	0.093700699009119	0.012096596364831843	6.264420696443212E-6
145-149	0.10392172781743574	0.012096596364831843	6.264420696443212E-6
150-154	0.114678991037368	0.012096596364831843	6.264420696443212E-6
155-159	0.12535732255652512	0.012096596364831843	6.264420696443212E-6
160-164	0.1360156079294536	0.012096596364831843	6.264420696443212E-6
165-169	0.14746321031013393	0.012096596364831843	7.517304835731855E-6
170-174	0.15926287313395435	0.012096596364831843	1.2528841392886424E-5
175-179	0.17109260517711772	0.012096596364831843	1.2528841392886424E-5
180-184	0.1832505928647747	0.012096596364831843	1.2528841392886424E-5
185-189	0.1961177129752691	0.012096596364831843	1.2528841392886424E-5
190-194	0.20954236652774685	0.012096596364831843	1.2528841392886424E-5
195-199	0.22322761998119672	0.012096596364831843	1.2528841392886424E-5
200-204	0.2379026519046846	0.012096596364831843	1.2528841392886424E-5
205-209	0.25263531649857973	0.012096596364831843	2.255191450719556E-5
210-214	0.26822244807546974	0.012096596364831843	2.5057682785772848E-5
215-219	0.2842806640887323	0.012096596364831843	2.8816335203638782E-5
220-224	0.30099664427512135	0.012096596364831843	3.1322103482216065E-5
225-229	0.31814111083714713	0.012096596364831843	3.1322103482216065E-5
230-234	0.3358393521887385	0.012096596364831843	3.1322103482216065E-5
235-239	0.35374056077089466	0.012096596364831843	3.7586524178659275E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACAGAG	313720	0.0	242.89922	1
TCAAACA	124710	0.0	242.28888	245
TACGAGG	102645	0.0	242.26701	1
CGTAGGG	290685	0.0	242.15117	3
GCAAACA	682000	0.0	242.00214	245
GTGCGAG	216160	0.0	241.95673	9
AGGATCC	14415	0.0	241.60074	6
GGTCCCA	32795	0.0	241.56346	7
TAGGGCG	46150	0.0	241.31038	5
AGGTCCC	51090	0.0	240.66008	6
GTAGGGT	168885	0.0	239.77022	4
TACGAAG	230565	0.0	239.27783	1
AGGGGGC	312345	0.0	239.23079	6
GTCCCAA	33120	0.0	239.08208	8
ACGAGGG	106050	0.0	238.57751	2
ACGGAGG	196790	0.0	238.55717	2
GGGTGCA	272575	0.0	238.29465	7
CGAAGGG	216435	0.0	237.97603	3
ACAGAGG	335300	0.0	237.1816	2
TACGGAG	193790	0.0	237.111	1
>>END_MODULE