##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AEB23 l01n03.3330000000879f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17176297 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.721456143894113 32.0 32.0 33.0 29.0 33.0 2 31.693648054641812 32.0 32.0 33.0 30.0 33.0 3 31.34241635435158 33.0 32.0 33.0 29.0 34.0 4 31.931720731191362 33.0 32.0 33.0 31.0 34.0 5 32.20140947725811 33.0 32.0 33.0 32.0 34.0 6 36.328217135509476 37.0 37.0 37.0 34.0 37.0 7 36.49930989199826 37.0 37.0 37.0 37.0 37.0 8 36.55354026540179 37.0 37.0 37.0 37.0 37.0 9 36.602472057859735 37.0 37.0 37.0 37.0 37.0 10-11 36.53960332660759 37.0 37.0 37.0 37.0 37.0 12-13 37.008659025865704 37.5 37.5 37.5 37.0 37.5 14-15 37.34210263714001 38.0 38.0 38.0 37.0 38.0 16-17 37.149419458687746 38.0 38.0 38.0 36.5 38.0 18-19 37.565391626611955 38.0 38.0 38.0 38.0 38.0 20-21 37.00238878612777 38.0 38.0 38.0 36.5 38.0 22-23 37.03849365785885 38.5 38.0 38.5 35.0 38.5 24-25 37.14982583265764 38.5 38.0 39.0 35.0 39.0 26-27 36.47839368404028 38.0 37.0 39.0 33.0 39.0 28-29 38.161723333032725 39.0 38.5 39.0 37.5 39.0 30-31 36.430362260270655 38.0 37.0 39.0 32.5 39.0 32-33 35.816465941407515 38.0 36.5 39.0 31.0 39.0 34-35 36.253979655801245 38.5 37.0 39.0 32.0 39.0 36-37 36.53731659973043 39.0 37.0 39.0 32.5 39.0 38-39 37.04543709275637 39.0 37.5 39.0 34.0 39.0 40-41 36.45201771371326 38.0 37.0 39.0 33.0 39.0 42-43 37.26614662636539 38.5 37.5 39.0 35.0 39.0 44-45 36.630471864802985 38.0 36.5 39.0 34.0 39.0 46-47 36.713288842175935 39.0 37.0 39.0 33.0 39.0 48-49 36.26736793151632 38.5 37.0 39.0 32.5 39.0 50-51 36.283981902502035 38.5 37.0 39.0 32.5 39.0 52-53 36.8086769517318 39.0 37.0 39.0 33.5 39.0 54-55 36.49004622474798 38.0 37.0 39.0 33.5 39.0 56-57 36.56474628961062 38.5 37.0 39.0 32.5 39.0 58-59 35.963156494091834 38.0 37.0 39.0 32.0 39.0 60-61 35.61685164153833 38.0 36.0 38.5 32.0 39.0 62-63 35.92388117764848 38.0 36.5 39.0 31.0 39.0 64-65 36.46440501698358 39.0 37.0 39.0 32.5 39.0 66-67 36.06248110404705 39.0 37.0 39.0 32.0 39.0 68-69 35.50270186292191 38.0 36.0 39.0 25.5 39.0 70-71 35.74406343229859 38.0 36.5 39.0 30.5 39.0 72-73 35.71481498602405 38.0 36.5 39.0 25.0 39.0 74-75 35.93271090386944 38.5 36.5 39.0 30.5 39.0 76-77 36.06934617513892 39.0 37.0 39.0 31.0 39.0 78-79 35.714365325657795 39.0 37.0 39.0 25.0 39.0 80-81 36.09748966846579 39.0 37.0 39.0 31.0 39.0 82-83 36.03539683204127 39.0 37.0 39.0 30.5 39.0 84-85 35.819931211017135 38.0 37.0 39.0 29.5 39.0 86-87 35.729547556146706 38.0 37.0 39.0 29.0 39.0 88-89 36.54236643672382 39.0 37.0 39.0 33.0 39.0 90-91 35.987114684847384 38.0 37.0 39.0 30.5 39.0 92-93 35.645590431977276 38.0 36.5 39.0 29.5 39.0 94-95 35.78081762908501 38.0 36.5 39.0 24.5 39.0 96-97 35.78276839879982 38.5 37.0 39.0 30.0 39.0 98-99 35.69778523857616 38.0 36.5 39.0 29.0 39.0 100-101 34.96892388970684 38.0 35.0 38.5 23.5 39.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6241431842668277 1101 2 0.3978281590147894 1101 3 0.5648529796789958 1101 4 0.3178731677879618 1101 5 0.2626985761520899 1101 6 0.0987031100254967 1101 7 0.05705804903411149 1101 8 0.07258616422191011 1101 9 0.0753701509074105 1101 10-11 0.07247827241229032 1101 12-13 0.09409640474043357 1101 14-15 0.15654709163071345 1101 16-17 0.1910823307168883 1101 18-19 0.10793915634076967 1101 20-21 0.3237873319081359 1101 22-23 0.2995925878621861 1101 24-25 0.5222051582590552 1101 26-27 0.6036618149696267 1101 28-29 0.17076341070614376 1101 30-31 0.5702858114179961 1101 32-33 0.6965822632774703 1101 34-35 0.7607620433136333 1101 36-37 0.5334179063225974 1101 38-39 0.45385989061378496 1101 40-41 0.4287628107708983 1101 42-43 0.2439571312485569 1101 44-45 0.38113226726863303 1101 46-47 0.49118594749403854 1101 48-49 0.6022184904425032 1101 50-51 0.433030529292175 1101 52-53 0.48346714356366505 1101 54-55 0.4360104806242262 1101 56-57 0.27554541821857725 1101 58-59 0.4310752403471696 1101 60-61 0.5896200473126356 1101 62-63 0.7718944482818131 1101 64-65 0.5027670712447829 1101 66-67 0.5844340329378284 1101 68-69 0.5182007172422161 1101 70-71 0.486226294619037 1101 72-73 0.46296942223996496 1101 74-75 0.6044075046282273 1101 76-77 0.4536712393932376 1101 78-79 0.7536973717877302 1101 80-81 0.4188318064587051 1101 82-83 0.29442881611522864 1101 84-85 0.5491881556107714 1101 86-87 0.6466718114383738 1101 88-89 0.37580959762318855 1101 90-91 0.4060228385603253 1101 92-93 0.6042107178987592 1101 94-95 0.6567232712032265 1101 96-97 0.6457516815888056 1101 98-99 0.5523122229370188 1101 100-101 0.6000991657518355 1102 1 0.5273867599329023 1102 2 0.40382558971844773 1102 3 0.6688955225202946 1102 4 0.3803844065904691 1102 5 0.3061453728532726 1102 6 0.08717681599024019 1102 7 0.06729547292103177 1102 8 0.08611115962087013 1102 9 0.08045035958927116 1102 10-11 0.10631686732044443 1102 12-13 0.12323159838806674 1102 14-15 0.1889169179124579 1102 16-17 0.2034642231486714 1102 18-19 0.11540759425259495 1102 20-21 0.2776606909007384 1102 22-23 0.27773056108568994 1102 24-25 0.49794624678870747 1102 26-27 0.643669215617038 1102 28-29 0.18594242260052596 1102 30-31 0.6251776629473582 1102 32-33 0.649600334302356 1102 34-35 0.7849043576986148 1102 36-37 0.5093260452934061 1102 38-39 0.49532489355539866 1102 40-41 0.42367954849323297 1102 42-43 0.24868582144497253 1102 44-45 0.39464662675892015 1102 46-47 0.6481333032978114 1102 48-49 0.6577772278284115 1102 50-51 0.4018205741779326 1102 52-53 0.5343560618604286 1102 54-55 0.5424224874400565 1102 56-57 0.3203942498419039 1102 58-59 0.4122600306232016 1102 60-61 0.6867199368236925 1102 62-63 0.8699620456529118 1102 64-65 0.5152391782504111 1102 66-67 0.6859004183863959 1102 68-69 0.5087375498067175 1102 70-71 0.5220122013270938 1102 72-73 0.5416918493428469 1102 74-75 0.7029370382773692 1102 76-77 0.47045635310247746 1102 78-79 0.9445957671229195 1102 80-81 0.5328910349260099 1102 82-83 0.5448097456079211 1102 84-85 0.7228681392774945 1102 86-87 0.784764853823539 1102 88-89 0.48966365368733733 1102 90-91 0.5414632180466512 1102 92-93 0.7849923292662595 1102 94-95 0.8421273693255458 1102 96-97 0.7630223806161354 1102 98-99 0.6936484181997074 1102 100-101 0.6991434467188569 1103 1 0.7345173524122117 1103 2 0.4878032717715435 1103 3 0.4984855738949534 1103 4 0.267151337802769 1103 5 0.22786423191870853 1103 6 0.1081229276827429 1103 7 0.06856499672551308 1103 8 -0.05502171181746718 1103 9 -0.03493646606487033 1103 10-11 -0.03013516697151175 1103 12-13 -0.070632017398502 1103 14-15 0.13870491676075147 1103 16-17 -0.15684837785160965 1103 18-19 0.06548310167176652 1103 20-21 -0.552441721036601 1103 22-23 0.07348177916204435 1103 24-25 -0.48418322805867575 1103 26-27 0.10195871319596961 1103 28-29 -0.21060195172604068 1103 30-31 -0.12781725546235378 1103 32-33 -0.45561745729091996 1103 34-35 -0.054397683587609436 1103 36-37 -0.7541662566896008 1103 38-39 -0.36540524649520734 1103 40-41 -0.49621905924244913 1103 42-43 0.0016221730678367408 1103 44-45 0.18026645042984768 1103 46-47 0.2337022644328428 1103 48-49 8.917193849100613E-5 1103 50-51 0.43793463252880827 1103 52-53 0.2694322726535603 1103 54-55 0.3990965859063138 1103 56-57 0.023965107878325398 1103 58-59 0.5055580861108808 1103 60-61 -0.6936261481048405 1103 62-63 0.4585700178077161 1103 64-65 -0.35420970077299785 1103 66-67 0.33220532236561695 1103 68-69 -0.7845974623065644 1103 70-71 0.004021324294420481 1103 72-73 0.28963234646224834 1103 74-75 0.544741997632201 1103 76-77 0.2974402103977454 1103 78-79 0.7781157739826199 1103 80-81 0.5570608465382847 1103 82-83 0.433494828229982 1103 84-85 0.2784199788969417 1103 86-87 0.6288946015547836 1103 88-89 0.20925007818343033 1103 90-91 0.5515832613052538 1103 92-93 0.7481019224039187 1103 94-95 0.6635836579325982 1103 96-97 0.5660587740948557 1103 98-99 0.5547646438876299 1103 100-101 0.6208818460123879 2112 1 0.7571062075615345 2112 2 0.44635466333601315 2112 3 0.5555253741073294 2112 4 0.3297471756583299 2112 5 0.26622672535425096 2112 6 0.09890712750613062 2112 7 0.06384756493221033 2112 8 0.10583595575875648 2112 9 0.08163180761509636 2112 10-11 0.12410945756001013 2112 12-13 0.0395842746183348 2112 14-15 0.13817873756430288 2112 16-17 0.151970479972924 2112 18-19 0.14613679562207693 2112 20-21 0.35047624381035547 2112 22-23 0.5089316598778382 2112 24-25 0.5129390401140839 2112 26-27 0.66551421190686 2112 28-29 0.18733795841279033 2112 30-31 0.5933763904671068 2112 32-33 0.6244537126374681 2112 34-35 0.7325841288421913 2112 36-37 0.43006670604263775 2112 38-39 0.5970383189999353 2112 40-41 0.5449463563252195 2112 42-43 0.25500257593048303 2112 44-45 0.32324447031928116 2112 46-47 0.5132831811107863 2112 48-49 0.6621982735150098 2112 50-51 0.5012377819215814 2112 52-53 0.5743484044015688 2112 54-55 0.4830566856040477 2112 56-57 0.39368706077501514 2112 58-59 0.6448489171250387 2112 60-61 0.7183114677074229 2112 62-63 0.8716793978414046 2112 64-65 0.4837899226259381 2112 66-67 0.6731923287666675 2112 68-69 0.4550537822218885 2112 70-71 0.5349179359089362 2112 72-73 0.5184387168521241 2112 74-75 0.7069443491404925 2112 76-77 0.3170571341872943 2112 78-79 0.7712402722514895 2112 80-81 0.6172375678542252 2112 82-83 0.4063150925228385 2112 84-85 0.5745956533858205 2112 86-87 0.793528450746102 2112 88-89 0.5219487152470847 2112 90-91 0.6308360318039448 2112 92-93 0.8024275973919259 2112 94-95 0.38549506716579884 2112 96-97 0.6569567283290638 2112 98-99 0.7679398278048808 2112 100-101 0.6423677368080618 1113 1 0.6004610159630204 1113 2 0.255268649660497 1113 3 0.28495336126795223 1113 4 0.21758320796105934 1113 5 0.1968019538658723 1113 6 0.0922569307682295 1113 7 0.06536902642884712 1113 8 0.09007499401830188 1113 9 0.08335789401222371 1113 10-11 0.12943562471156156 1113 12-13 0.1621506351735178 1113 14-15 0.16860174475064582 1113 16-17 0.12634805667877202 1113 18-19 0.1535448804141879 1113 20-21 0.416250713490939 1113 22-23 0.4734989697971841 1113 24-25 0.4637739887502903 1113 26-27 0.7283686543656955 1113 28-29 0.20949351608291522 1113 30-31 0.4908180680019498 1113 32-33 0.6204518003760526 1113 34-35 0.7989160642486581 1113 36-37 0.4272782329246354 1113 38-39 0.5391714753282812 1113 40-41 0.5284830196200403 1113 42-43 0.3107545501076814 1113 44-45 0.34439072360542866 1113 46-47 0.5044858645892489 1113 48-49 0.7439220008899383 1113 50-51 0.5502748401709354 1113 52-53 0.5273834214384152 1113 54-55 0.5586877508717336 1113 56-57 0.5281944898595299 1113 58-59 0.658568639461464 1113 60-61 0.567475132300487 1113 62-63 0.9081956812054983 1113 64-65 0.5887038102728255 1113 66-67 0.7616129266997334 1113 68-69 0.626067436307089 1113 70-71 0.6708565555226045 1113 72-73 0.5647805295362645 1113 74-75 0.657482123837255 1113 76-77 0.47779396595579016 1113 78-79 0.8070773669866682 1113 80-81 0.6065690414226523 1113 82-83 0.4080752439083284 1113 84-85 0.5746579140629962 1113 86-87 0.8362076474948736 1113 88-89 0.41124878285779687 1113 90-91 0.6325868615867023 1113 92-93 0.5828857247021233 1113 94-95 0.5667443790319382 1113 96-97 0.6600207431262248 1113 98-99 0.7421798928696433 1113 100-101 0.6895212739941172 2113 1 0.34392182278735106 2113 2 0.3247703317341326 2113 3 0.4989101065775081 2113 4 0.2593703094146882 2113 5 0.20954796560307898 2113 6 0.06425090018568369 2113 7 0.039933765765375995 2113 8 0.07773474558916149 2113 9 0.06746102663315412 2113 10-11 0.06542369111390656 2113 12-13 0.10788625300285304 2113 14-15 0.08629034141838332 2113 16-17 0.09477909003085472 2113 18-19 0.11404218725176207 2113 20-21 0.36856254604744265 2113 22-23 0.42286813686417446 2113 24-25 0.4486209609237477 2113 26-27 0.6113825114472746 2113 28-29 0.1748816068232415 2113 30-31 0.42470861074935584 2113 32-33 0.6000463867608445 2113 34-35 0.5731770425579441 2113 36-37 0.5108556721159019 2113 38-39 0.53000571303388 2113 40-41 0.531186040261808 2113 42-43 0.1230485277766249 2113 44-45 0.070859220691041 2113 46-47 0.4340435035240304 2113 48-49 0.5975234818446395 2113 50-51 0.4477168208167157 2113 52-53 0.45041300918109073 2113 54-55 0.3919778151957942 2113 56-57 0.25761084245817045 2113 58-59 0.42911034653914726 2113 60-61 0.32714443148474004 2113 62-63 0.6158241540075409 2113 64-65 0.32088571728778703 2113 66-67 0.483587377210867 2113 68-69 0.24829859646526842 2113 70-71 0.15122536623295701 2113 72-73 0.35983560615152044 2113 74-75 0.51531336400285 2113 76-77 0.4054462607468565 2113 78-79 0.7819737119458594 2113 80-81 0.4411949925982199 2113 82-83 0.39440620057321496 2113 84-85 0.5567384222158367 2113 86-87 0.652144619786938 2113 88-89 0.336052588680694 2113 90-91 0.5990057548826044 2113 92-93 0.46771030494312527 2113 94-95 0.3450519002558039 2113 96-97 0.5859629726051026 2113 98-99 0.6779953828401446 2113 100-101 0.6145438490151562 1112 1 0.4430481667026598 1112 2 0.3308719934996738 1112 3 0.5292336412654421 1112 4 0.2742299694407926 1112 5 0.18252351933656286 1112 6 0.03885615993387859 1112 7 0.009117033477508585 1112 8 0.0366488915599561 1112 9 0.031401917568970816 1112 10-11 0.05783197783617311 1112 12-13 0.07671521450637186 1112 14-15 0.1033674641780209 1112 16-17 0.08966787546490451 1112 18-19 0.08847394037905332 1112 20-21 0.3443534239830228 1112 22-23 0.34188913726035963 1112 24-25 0.4181728802334206 1112 26-27 0.41866102549876416 1112 28-29 0.1148422823364541 1112 30-31 0.4195518199448358 1112 32-33 0.4903563482351032 1112 34-35 0.4846728454843756 1112 36-37 0.303165443187531 1112 38-39 0.3715676021951779 1112 40-41 0.31679524191174835 1112 42-43 0.11197160791059702 1112 44-45 0.0656799223831186 1112 46-47 0.33012610562867195 1112 48-49 0.352706720257423 1112 50-51 0.3683404750356374 1112 52-53 0.26834867418246233 1112 54-55 0.26531394270258346 1112 56-57 0.17909101382115722 1112 58-59 0.29871307412672365 1112 60-61 0.12165469792920902 1112 62-63 0.519229456117472 1112 64-65 0.15852403882381338 1112 66-67 0.44531549808413473 1112 68-69 0.1565029856684106 1112 70-71 0.30818059221979865 1112 72-73 0.30529399488167996 1112 74-75 0.37221320674714775 1112 76-77 0.3437172534320112 1112 78-79 0.7329403140826045 1112 80-81 0.4101329814009915 1112 82-83 0.387352840385887 1112 84-85 0.37375789473149723 1112 86-87 0.3902004283339622 1112 88-89 0.294005131836208 1112 90-91 0.3360619778769163 1112 92-93 0.0992871645860589 1112 94-95 0.49859014622335707 1112 96-97 0.3968273007785825 1112 98-99 0.39133187214108034 1112 100-101 0.3001997436728914 2114 1 0.5875484740948806 2114 2 0.40309663067176515 2114 3 0.4809415941007025 2114 4 0.2601758592611638 2114 5 0.28258276918727887 2114 6 0.07774953244012295 2114 7 0.040197240313290195 2114 8 0.07401302062587689 2114 9 0.07755947632698224 2114 10-11 0.09993741722321658 2114 12-13 0.1105902489777435 2114 14-15 0.15222079578700942 2114 16-17 0.152866639320429 2114 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2109 16-17 -0.23727288493721943 2109 18-19 -0.1719810982271497 2109 20-21 -0.5326347461852308 2109 22-23 -0.7283303294039456 2109 24-25 -0.7048501532231839 2109 26-27 -0.9935281093280821 2109 28-29 -0.177621498858052 2109 30-31 -0.8032637935944464 2109 32-33 -1.1172302536646797 2109 34-35 -1.3373295394078468 2109 36-37 -0.9142657667662206 2109 38-39 -0.7661100080648495 2109 40-41 -0.8054566959196237 2109 42-43 -0.4801499909228255 2109 44-45 -0.5839956443688408 2109 46-47 -1.2672250058812509 2109 48-49 -1.083246041156933 2109 50-51 -0.9698522622665493 2109 52-53 -0.8626174420278829 2109 54-55 -0.5234723895934721 2109 56-57 -0.7857307381441885 2109 58-59 -1.2208496215870284 2109 60-61 -0.6900721837076418 2109 62-63 -0.6051400383158381 2109 64-65 -0.6215456614940891 2109 66-67 -0.7936470791894124 2109 68-69 -0.5649039811319838 2109 70-71 -0.7510915318994051 2109 72-73 -0.9155982159003884 2109 74-75 -0.5070111516566769 2109 76-77 -0.5856158934247802 2109 78-79 -0.24611630847135757 2109 80-81 -0.7469917530412644 2109 82-83 -0.6748131964478787 2109 84-85 -1.0569855130149222 2109 86-87 -1.7221487644295834 2109 88-89 -0.856808188651911 2109 90-91 -1.2880292441767338 2109 92-93 -0.952512299812895 2109 94-95 -1.724820816438971 2109 96-97 -1.367108338313706 2109 98-99 -1.5196707204414466 2109 100-101 -0.9273779489537262 2108 1 -1.1356879939629607 2108 2 -0.6220102048752523 2108 3 -1.8983968857391709 2108 4 -0.7151790828961246 2108 5 -0.4325153804569837 2108 6 -0.11981899799070561 2108 7 -0.06307542888086459 2108 8 -0.15774931320551389 2108 9 -0.13333705673565532 2108 10-11 -0.12014137865655528 2108 12-13 -0.09492566101092592 2108 14-15 -0.24898030369500646 2108 16-17 -0.35364298793677307 2108 18-19 -0.20340628855068132 2108 20-21 -0.8179519537950029 2108 22-23 -1.480336777298227 2108 24-25 -1.1331381360940114 2108 26-27 -1.4915161185846983 2108 28-29 -0.28777862054919723 2108 30-31 -2.064676624309662 2108 32-33 -2.2715294773225168 2108 34-35 -2.166299074993084 2108 36-37 -1.4601428778466143 2108 38-39 -1.244178094491538 2108 40-41 -1.4506070067297046 2108 42-43 -0.6966918371293858 2108 44-45 -1.1221349211612974 2108 46-47 -1.0792020565014724 2108 48-49 -1.5711373211835777 2108 50-51 -1.3593570855610224 2108 52-53 -1.6413692621933507 2108 54-55 -1.0605270925342438 2108 56-57 -1.1947132131572786 2108 58-59 -1.440501997904299 2108 60-61 -1.294263699654536 2108 62-63 -1.162885195823371 2108 64-65 -1.0700892540249356 2108 66-67 -1.3060028653017426 2108 68-69 -1.134479748380663 2108 70-71 -1.6777678261725413 2108 72-73 -1.2720365523271155 2108 74-75 -1.122090234951166 2108 76-77 -0.8903752140004215 2108 78-79 -0.514619003268507 2108 80-81 -0.7397442354142569 2108 82-83 -1.4212471082824862 2108 84-85 -1.6647961007831213 2108 86-87 -1.6900709559386655 2108 88-89 -0.8010334168964874 2108 90-91 -1.19212073396357 2108 92-93 -1.1243276254544483 2108 94-95 -0.9955844415986661 2108 96-97 -1.478838919073283 2108 98-99 -1.4221542366138777 2108 100-101 -1.1272295866288644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 23.0 14 177.0 15 1825.0 16 1220.0 17 2993.0 18 12536.0 19 29657.0 20 38572.0 21 30225.0 22 24007.0 23 27459.0 24 37682.0 25 54655.0 26 81543.0 27 129423.0 28 190343.0 29 262674.0 30 357791.0 31 473062.0 32 530005.0 33 637692.0 34 843929.0 35 1219046.0 36 2069944.0 37 5804344.0 38 4315469.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 62.93795455446538 28.48432930567048 5.767989456633173 2.8097266832309664 2 92.52530973352405 2.428352281053361 1.304687500454842 3.7416504849677437 3 5.601667227808182 36.39638974570596 45.29203238625881 12.70991064022705 4 1.5973873763361217 12.999443360812869 84.18742992159486 1.2157393412561508 5 1.096988483606216 95.90407641414211 1.430418908103417 1.56851619414825 6 1.035729705636186 1.681584900493098 1.3001289684341724 95.98255642543654 7 1.291465866680453 1.2870819180107282 1.2422876270879357 96.17916458822087 8 0.9588620876781532 1.5798574046547984 95.95858758147929 1.5026929261877575 9 1.027392574779069 1.521841407376689 96.19809787872205 1.2526681391221868 10-11 48.46094000708884 1.418559624263578 48.99697816106569 1.1235222075818907 12-13 1.2048435119630267 48.49142105542306 48.98391370386761 1.3198217287463065 14-15 48.61131593148395 1.2750041525248428 1.628855160108142 48.48482475588307 16-17 1.2273221637935114 48.337182339127 1.2154686193421085 49.22002687773738 18-19 1.154882219374758 1.2032803112335562 96.32266489104143 1.3191725783502697 20-21 1.275670768850818 28.298785820948485 30.789508937811217 39.63603447238948 22-23 15.98256306350548 5.056328497347246 13.51164048921604 65.44946794993123 24-25 0.9973337093553982 1.56707816591667 39.84662410064288 57.58896402408505 26-27 41.262194057310495 26.594370136939293 11.385483727953703 20.757952077796514 28-29 0.989325580478726 1.6073516893658744 51.154346597523315 46.24897613263208 30-31 11.164464028538863 38.66116777091128 26.183111528637397 23.99125667191246 32-33 29.6955653479909 51.112390522823404 12.901078154389156 6.290965974796546 34-35 20.458262918951622 39.1261486687148 31.129582237661584 9.286006174671991 36-37 12.260812094714012 26.275945857247347 30.155446194252463 31.307795853786182 38-39 16.10035038402049 49.93755930047087 6.491046935203787 27.47104338030485 40-41 33.9585476427195 12.118546273390592 13.249072253466506 40.6738338304234 42-43 27.194013354566472 5.5297017744860835 65.65077152543415 1.6255133455132968 44-45 57.76059880659958 17.52735179183266 19.972081293191426 4.739968108376328 46-47 5.48207509453289 47.813416943128075 32.52142763949645 14.183080322842578 48-49 31.677395308197102 34.60441735491649 28.74008291775579 4.97810441913062 50-51 24.10852583650597 18.36452583464294 34.85251215672388 22.674436172127205 52-53 11.642841294605 2.034021069849922 55.711044703057944 30.612092932487133 54-55 2.2286148172682387 12.29011119218537 35.16540555860207 50.315868431944324 56-57 14.938455593775538 9.592719548340368 23.413751520481977 52.05507333740211 58-59 17.269333430832035 5.952741152531305 38.81172990895534 37.96619550768131 60-61 19.53217273781421 42.690639315330884 14.442533801086462 23.33465414576844 62-63 34.79283980708997 21.955570516741762 25.967523733433346 17.284065942734923 64-65 13.202828292966757 18.22101702130558 52.6613274095109 15.914827276216753 66-67 16.527005209562923 30.028957929639898 15.27071580096688 38.1733210598303 68-69 31.46422363330117 30.501964422250033 5.995113498561419 32.03869844588738 70-71 11.862222107593972 34.399501242904684 29.041367298201703 24.69690935129964 72-73 13.062833042535305 21.42729308884214 20.583033700453594 44.92684016816896 74-75 23.666928907901394 43.92325947787233 22.23000102990767 10.179810584318611 76-77 9.150627751720874 20.27555473685626 35.476176849992754 35.097640661430106 78-79 17.77150511545067 26.281363206516517 24.271046314581078 31.67608536345174 80-81 32.896773384857056 22.097262873365544 33.27680873240606 11.729155009371345 82-83 30.035699778596047 13.952567890506318 36.237065532809545 19.774666798088088 84-85 26.234799037301233 26.81841435322177 23.808726060104807 23.1380605493722 86-87 4.705391389075305 40.60603691237989 22.878959300715398 31.809612397829408 88-89 7.720243775477334 16.235460996045887 5.3116425501957725 70.732652678281 90-91 9.726939397938915 41.43878043096251 8.05049540072578 40.7837847703728 92-93 17.501476016629194 16.928331525706618 32.78976254311392 32.78042991455027 94-95 13.44350007455041 11.740729098943737 17.746962572899154 57.0688082536067 96-97 16.49340367135012 28.48913243640349 28.415720221884843 26.601743670361543 98-99 34.885566429131956 17.436511490224 30.29237908496808 17.38554299567596 100-101 34.009664597672014 12.194197037929653 38.641603018392146 15.154535346006185 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 2.0 2 0.5 3 0.0 4 0.0 5 0.5 6 1.0 7 1.5 8 1.5 9 2.5 10 4.5 11 7.5 12 14.5 13 16.0 14 17.0 15 25.5 16 48.0 17 77.0 18 91.5 19 128.0 20 191.5 21 249.5 22 605.5 23 954.0 24 1158.5 25 1587.5 26 2981.5 27 6704.5 28 8662.0 29 12577.5 30 19252.0 31 22206.5 32 39005.5 33 153001.5 34 176341.0 35 84795.5 36 88839.5 37 229966.0 38 273555.5 39 171967.0 40 141262.0 41 175636.0 42 316706.5 43 936962.0 44 1557279.0 45 1123512.5 46 611133.5 47 549010.0 48 385530.5 49 526322.0 50 1785851.5 51 3200966.5 52 2271783.0 53 683064.5 54 514305.0 55 480084.0 56 232886.5 57 133665.5 58 95646.5 59 48668.0 60 29609.0 61 20885.5 62 18452.0 63 16919.0 64 11615.5 65 4326.5 66 2394.5 67 1528.0 68 997.0 69 708.0 70 598.5 71 530.5 72 396.0 73 276.5 74 196.0 75 128.5 76 96.0 77 88.5 78 69.5 79 57.5 80 48.5 81 46.5 82 52.0 83 43.0 84 33.0 85 29.0 86 28.5 87 27.0 88 24.0 89 22.5 90 21.5 91 21.0 92 26.5 93 27.5 94 26.0 95 28.5 96 24.5 97 26.0 98 45.0 99 65.0 100 238.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 5.821976646072201E-6 7 5.821976646072201E-6 8 0.0 9 0.0 10-11 5.821976646072201E-6 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7176297E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 3.8062963448559937 #Duplication Level Percentage of deduplicated Percentage of total 1 86.77962937519065 3.30308986098746 2 4.86650555593916 0.3704672461958522 3 1.7321986357972208 0.197797840079985 4 1.0754160472404106 0.1637340867924262 5 0.7259790031959749 0.13816456131535185 6 0.4925572879134315 0.11248914027702456 7 0.34712451277321565 0.09248811349250259 8 0.24253413837982707 0.07385254435343469 9 0.2668575249873788 0.09141649397610993 >10 2.1334507559238847 2.091607262286389 >50 0.8724462950427712 2.2517411258228335 >100 0.30988625906018036 2.4581702253255115 >500 0.061221768461195314 1.636755865641306 >1k 0.067846729768412 5.612383352613272 >5k 0.012787878112257487 3.2512658476968577 >10k+ 0.01355823221415673 78.15457643314386 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT 2855465 16.624450543676556 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC 2517925 14.659300546561346 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT 1915927 11.154482249579173 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC 673052 3.918493025592187 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGT 667635 3.8869553781004136 No Hit TCGTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTAAAGTG 407586 2.372956173265984 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC 339797 1.9782901984053956 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTAATGATTAAATATCGGTTAT 335515 1.9533604944069145 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGCAAAGGTTTACTTAA 265518 1.5458395951117987 No Hit AGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG 265142 1.5436505318928755 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC 261576 1.522889363172982 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA 201806 1.1749098190372465 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTGTTTAA 108731 0.6330293427040765 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGAA 102232 0.5951923164812533 No Hit AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAAGTG 100144 0.5830360292442545 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATCGACCACACCGTGGTAAGC 98253 0.5720266714065319 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTAAATCCACTGTGGAAGGT 96670 0.5628104823757996 No Hit GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATATAAACATTCAATAGT 95526 0.556150141092693 No Hit AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAACTG 85144 0.49570637955317143 No Hit TGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGGT 84012 0.4891159019898177 No Hit CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTCGAGGTA 78306 0.45589570324732975 No Hit TGCTACCTTGTTACGACTTCACCCCAGTCATGAAACATACCGTGGTAACC 72452 0.42181385196122306 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC 68998 0.4017047446256897 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAGACGATAAGGTTTGAGGAG 58039 0.33790170256138446 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGC 57340 0.33383214088577995 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGCAAAGGTTTACTTAA 53202 0.3097408015243332 No Hit GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCATACCGATCAAGATC 52650 0.3065270704157014 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATATGGTTTACTTGA 50988 0.2968509452299294 No Hit CGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG 50836 0.2959660047797264 No Hit CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTTGAGGTA 46785 0.27238117738648787 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC 46735 0.2720900785541843 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC 45601 0.2654879570375384 No Hit CAAGCAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGG 44070 0.25657451079240184 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC 42998 0.25033335182781247 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTTAAAAAA 42378 0.2467237263072477 No Hit GGTTACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT 37581 0.21879570433603937 No Hit AGCTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATCGGAGTA 34735 0.20222635880131787 No Hit AGCAACCTTGTTACGACTTCTACCATGTCAAGATCCCAAGTATCGTTACA 34602 0.20145203590739028 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACGAAA 34486 0.2007766866164459 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC 33165 0.19308585546698454 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTAGAAAAG 32701 0.19038445830320702 No Hit GAATACCTTGTTACGACTTTTACTTCCTCTAAATGATCCAGTTTGAGCAT 29632 0.17251681197641144 No Hit TGCTACCTTGTTACGACTTCACCCTAGTCATGAAACATACCGTGGTAACC 29227 0.1701589114347522 No Hit GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA 28195 0.1641506315360057 No Hit GGAAACCTTGTTACGACTTTTACTTTCTCTAGATGGTCAGGTTTGGGCAT 27504 0.1601276456735698 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGTTAAGGTTTACTTAA 27401 0.15952798207902438 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACAGTGGTAAGC 26054 0.15168577953676513 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAAGC 24156 0.14063566786252008 No Hit GGAAACCTTGTTACGACTTTTGCTTCCTCTAGATGATAAGGTTTAATCAA 23217 0.13516883179185826 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAACCCACCGTGGTCGGT 22866 0.13312531798908694 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGTCTAT 22090 0.1286074641117349 No Hit GAAGACCTTGTTACGACTTCTCCTTCTTCTAAATTGCAAGATTCGAACAA 22080 0.12854924434527418 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTAGAAAAA 21977 0.12794958075072874 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCCGCCGTGGTAAGC 21902 0.12751293250227333 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGC 20542 0.11959504426361514 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACTTAA 20443 0.11901866857565399 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGATTAAATATCGGTTAT 20428 0.11893133892596292 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCCGAATCCACTGTGGAAGGT 19994 0.11640460106156758 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT 19170 0.1116072923052041 No Hit CGGTACCTTGTTACGACTTTTACCGGGTTCAAGCCGCGGACGATCAGAAA 18627 0.10844595898638687 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAATC 18398 0.10711272633443636 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAG 18029 0.10496441695203572 No Hit GGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT 17403 0.10131985957159449 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.3287906584288802E-4 0.0 0.0 2 2.4452301913503244E-4 0.0 0.0 3 2.503449957811046E-4 0.0 0.0 4 2.561669724271768E-4 0.0 0.0 5 2.561669724271768E-4 0.0 0.0 6 2.6781092571932124E-4 0.0 0.0 7 2.6781092571932124E-4 0.0 0.0 8 2.7363290236539345E-4 0.0 0.0 9 2.794548790114656E-4 0.0 0.0 10-11 2.852768556575378E-4 0.0 0.0 12-13 3.085647622418266E-4 0.0 0.0 14-15 3.347636571491515E-4 0.0 0.0 16-17 3.4931859876433205E-4 0.0 0.0 18-19 3.580515637334403E-4 0.0 0.0 20-21 3.871614469638013E-4 0.0 0.0 22-23 3.9589441193290965E-4 0.0 0.0 24-25 4.017163885789818E-4 0.0 0.0 26-27 4.07538365225054E-4 0.0 0.0 28-29 4.1918231851719844E-4 0.0 5.821976646072201E-6 30-31 4.6284714336273993E-4 0.0 5.821976646072201E-6 32-33 4.91957026593101E-4 0.0 5.821976646072201E-6 34-35 4.948680149161371E-4 0.0 5.821976646072201E-6 36-37 5.152449331773897E-4 2.9109883230361004E-6 2.03769182612527E-5 38-39 5.443548164077508E-4 5.821976646072201E-6 5.2397789814649805E-5 40-41 6.025745828684727E-4 5.821976646072201E-6 6.98637197528664E-5 42-43 6.66616325975267E-4 5.821976646072201E-6 9.897360298322741E-5 44-45 7.481239990202778E-4 5.821976646072201E-6 1.4554941615180502E-4 46-47 8.092547538040358E-4 3.49318598764332E-5 1.804812760282382E-4 48-49 9.460712049867326E-4 3.49318598764332E-5 2.4452301913503244E-4 50-51 0.0011178195160458627 3.49318598764332E-5 3.056537739187905E-4 52-53 0.0012895678271049923 3.49318598764332E-5 3.3767464547218763E-4 54-55 0.0014671381148101945 3.49318598764332E-5 4.017163885789818E-4 56-57 0.001653441367484505 3.49318598764332E-5 4.5120319007059556E-4 58-59 0.0018921424099734652 3.49318598764332E-5 5.443548164077508E-4 60-61 0.0021570423473697502 3.49318598764332E-5 6.054855711915088E-4 62-63 0.0024510521679963967 3.49318598764332E-5 6.782602792674114E-4 64-65 0.0027188630937157177 3.49318598764332E-5 7.073701624977723E-4 66-67 0.002975030066142894 3.49318598764332E-5 7.685009172815305E-4 68-69 0.003335992618199371 3.49318598764332E-5 8.092547538040358E-4 70-71 0.003734798018455317 3.49318598764332E-5 8.67474520264758E-4 72-73 0.0041277814420651905 3.49318598764332E-5 9.198723100794076E-4 74-75 0.004613916492012219 3.49318598764332E-5 9.780920765401298E-4 76-77 0.00510587351860532 3.49318598764332E-5 0.001042133819646924 78-79 0.005574542638614132 3.49318598764332E-5 0.0010887096328155016 80-81 0.006121808443344919 3.49318598764332E-5 0.0011236414926919347 82-83 0.006817534652550547 3.49318598764332E-5 0.0011556623642453318 84-85 0.007423020223742056 3.49318598764332E-5 0.0011847722474756929 86-87 0.007990662946734096 3.49318598764332E-5 0.0012284370723212343 88-89 0.008773718805630805 3.49318598764332E-5 0.0012517249789055231 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTTGT 1601740 0.0 94.96798 5 TGTTACG 1601500 0.0 94.95047 9 CTTGTTA 1600820 0.0 94.94689 7 GATACCT 391230 0.0 94.92959 2 AGATACC 356880 0.0 94.92812 1 TTGTTAC 1600995 0.0 94.92138 8 GTTACCT 747250 0.0 94.89067 2 CCTTGTT 1605425 0.0 94.8852 6 TACCTTG 1403135 0.0 94.86019 4 TCGTACC 48430 0.0 94.85288 1 ATACCTT 395790 0.0 94.66516 3 GGTTACC 744840 0.0 94.45157 1 TTACCTT 750865 0.0 94.373726 3 AACCTTG 198335 0.0 94.28871 4 AGGTACC 23000 0.0 94.21522 1 CTACCTT 190235 0.0 94.17352 3 GAAACCT 188675 0.0 94.08109 2 GGTACCT 26300 0.0 94.02472 2 GGAAACC 186200 0.0 93.95153 1 CGTACCT 49260 0.0 93.70788 2 >>END_MODULE