FastQCFastQC Report
Thu 16 Apr 2015
000000000-AEB23 l01n03.3330000000879f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AEB23 l01n03.3330000000879f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17176297
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT285546516.624450543676556No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC251792514.659300546561346No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT191592711.154482249579173No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC6730523.918493025592187No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGT6676353.8869553781004136No Hit
TCGTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTAAAGTG4075862.372956173265984No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC3397971.9782901984053956No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTAATGATTAAATATCGGTTAT3355151.9533604944069145No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGCAAAGGTTTACTTAA2655181.5458395951117987No Hit
AGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG2651421.5436505318928755No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC2615761.522889363172982No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA2018061.1749098190372465No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTGTTTAA1087310.6330293427040765No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGAA1022320.5951923164812533No Hit
AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAAGTG1001440.5830360292442545No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCGACCACACCGTGGTAAGC982530.5720266714065319No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTAAATCCACTGTGGAAGGT966700.5628104823757996No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATATAAACATTCAATAGT955260.556150141092693No Hit
AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAACTG851440.49570637955317143No Hit
TGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGGT840120.4891159019898177No Hit
CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTCGAGGTA783060.45589570324732975No Hit
TGCTACCTTGTTACGACTTCACCCCAGTCATGAAACATACCGTGGTAACC724520.42181385196122306No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC689980.4017047446256897No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAGACGATAAGGTTTGAGGAG580390.33790170256138446No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGC573400.33383214088577995No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGCAAAGGTTTACTTAA532020.3097408015243332No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCATACCGATCAAGATC526500.3065270704157014No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATATGGTTTACTTGA509880.2968509452299294No Hit
CGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG508360.2959660047797264No Hit
CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTTGAGGTA467850.27238117738648787No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC467350.2720900785541843No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC456010.2654879570375384No Hit
CAAGCAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGG440700.25657451079240184No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC429980.25033335182781247No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTTAAAAAA423780.2467237263072477No Hit
GGTTACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT375810.21879570433603937No Hit
AGCTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATCGGAGTA347350.20222635880131787No Hit
AGCAACCTTGTTACGACTTCTACCATGTCAAGATCCCAAGTATCGTTACA346020.20145203590739028No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACGAAA344860.2007766866164459No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC331650.19308585546698454No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTAGAAAAG327010.19038445830320702No Hit
GAATACCTTGTTACGACTTTTACTTCCTCTAAATGATCCAGTTTGAGCAT296320.17251681197641144No Hit
TGCTACCTTGTTACGACTTCACCCTAGTCATGAAACATACCGTGGTAACC292270.1701589114347522No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA281950.1641506315360057No Hit
GGAAACCTTGTTACGACTTTTACTTTCTCTAGATGGTCAGGTTTGGGCAT275040.1601276456735698No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGTTAAGGTTTACTTAA274010.15952798207902438No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACAGTGGTAAGC260540.15168577953676513No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAAGC241560.14063566786252008No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAGATGATAAGGTTTAATCAA232170.13516883179185826No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAACCCACCGTGGTCGGT228660.13312531798908694No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGTCTAT220900.1286074641117349No Hit
GAAGACCTTGTTACGACTTCTCCTTCTTCTAAATTGCAAGATTCGAACAA220800.12854924434527418No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTAGAAAAA219770.12794958075072874No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCCGCCGTGGTAAGC219020.12751293250227333No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGC205420.11959504426361514No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACTTAA204430.11901866857565399No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGATTAAATATCGGTTAT204280.11893133892596292No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCCGAATCCACTGTGGAAGGT199940.11640460106156758No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT191700.1116072923052041No Hit
CGGTACCTTGTTACGACTTTTACCGGGTTCAAGCCGCGGACGATCAGAAA186270.10844595898638687No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAATC183980.10711272633443636No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAG180290.10496441695203572No Hit
GGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT174030.10131985957159449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTGT16017400.094.967985
TGTTACG16015000.094.950479
CTTGTTA16008200.094.946897
GATACCT3912300.094.929592
AGATACC3568800.094.928121
TTGTTAC16009950.094.921388
GTTACCT7472500.094.890672
CCTTGTT16054250.094.88526
TACCTTG14031350.094.860194
TCGTACC484300.094.852881
ATACCTT3957900.094.665163
GGTTACC7448400.094.451571
TTACCTT7508650.094.3737263
AACCTTG1983350.094.288714
AGGTACC230000.094.215221
CTACCTT1902350.094.173523
GAAACCT1886750.094.081092
GGTACCT263000.094.024722
GGAAACC1862000.093.951531
CGTACCT492600.093.707882
Produced by FastQC (version 0.11.2)