##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AEB23 l01n02.33200000008792.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17176297 Sequences flagged as poor quality 0 Sequence length 12 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.946895829758883 33.0 32.0 34.0 16.0 34.0 2 31.07518360913298 33.0 32.0 34.0 16.0 35.0 3 31.13089404543948 33.0 32.0 34.0 16.0 35.0 4 31.152490493148786 33.0 32.0 34.0 16.0 35.0 5 31.509172844414604 33.0 32.0 34.0 18.0 35.0 6 33.06650088782233 35.0 33.0 37.0 18.0 37.0 7 33.038407929252735 36.0 33.0 37.0 18.0 37.0 8 32.96835872132392 36.0 33.0 37.0 18.0 37.0 9 32.46036465252085 34.0 32.0 37.0 18.0 37.0 10 32.66909672090556 34.0 33.0 37.0 18.0 37.0 11 32.730374597039166 35.0 33.0 37.0 18.0 37.0 12 33.0459760331345 37.0 33.0 37.0 18.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05966136795583665 1101 2 0.04947869645044278 1101 3 0.2166143704521879 1101 4 0.2507690349994576 1101 5 0.29694003684013026 1101 6 0.35426618669668386 1101 7 0.31779339421787256 1101 8 0.29323134070844503 1101 9 0.3464177217810871 1101 10 0.2995315938994878 1101 11 0.3853341106731065 1101 12 0.3428550638848904 1102 1 0.29754720004584456 1102 2 0.37941621143014004 1102 3 0.4409348287219075 1102 4 0.375623486090479 1102 5 0.36893353340406776 1102 6 0.4361124534934504 1102 7 0.4134477428797396 1102 8 0.26647461765415414 1102 9 0.3063209913372944 1102 10 0.25365096913838414 1102 11 0.40878076738480473 1102 12 0.321798708266293 1103 1 0.4745036512313945 1103 2 0.4595170166193334 1103 3 0.30870143522615834 1103 4 0.39283416433623586 1103 5 0.3438887473731995 1103 6 0.260924840163824 1103 7 0.4058198750783575 1103 8 0.1402609131311081 1103 9 -0.02033002939091233 1103 10 0.18522159034179708 1103 11 0.3111200746469862 1103 12 -0.03549180773133287 2112 1 0.44455081590552226 2112 2 0.41039664774661233 2112 3 0.47380879648993357 2112 4 0.39414699661451635 2112 5 0.32259189979459535 2112 6 0.12314123061398874 2112 7 0.30935677393096483 2112 8 0.1819579967353846 2112 9 0.27180200799973164 2112 10 0.30624451505262584 2112 11 0.26644014851164144 2112 12 0.08575149825306738 1113 1 0.4761565516458077 1113 2 0.45554000051810917 1113 3 0.48633988094363545 1113 4 0.3732223698646031 1113 5 0.37089751096122825 1113 6 0.23035980011017898 1113 7 0.32494990666904044 1113 8 0.3587494663984998 1113 9 0.39170556450205396 1113 10 0.3520434641243 1113 11 0.32334137541509733 1113 12 0.36120428875143773 2113 1 0.3314587478909381 2113 2 0.3118053438809376 2113 3 0.3250286761449743 2113 4 0.24010441746357003 2113 5 0.18809187264111316 2113 6 -0.06436629192458554 2113 7 0.14960182662625954 2113 8 -0.27096881429810793 2113 9 0.14796911716809547 2113 10 0.20646464815948207 2113 11 0.25440354242086727 2113 12 0.18207189140414926 1112 1 0.3612449487275491 1112 2 0.3338613537665225 1112 3 0.3512261735118436 1112 4 0.28412467689319953 1112 5 0.23586382170178055 1112 6 0.2658722840823202 1112 7 0.256084201739057 1112 8 0.23689178746882789 1112 9 0.2319097202377094 1112 10 0.18391786650752806 1112 11 0.3394213374472983 1112 12 0.23091336147285801 2114 1 0.4331911071102681 2114 2 0.34147676814323447 2114 3 0.37365024445854544 2114 4 0.28982137625273197 2114 5 0.21069829502346238 2114 6 0.2766876569464287 2114 7 0.2817798074261191 2114 8 0.19127351192467756 2114 9 0.22577049457300546 2114 10 0.19695743780363983 2114 11 0.3009046057021152 2114 12 0.2606969367264895 1114 1 0.4251542326101543 1114 2 0.406606152332472 1114 3 0.38209320234812694 1114 4 0.2650997927288614 1114 5 0.3022599815093905 1114 6 0.35861118552234217 1114 7 0.3139950067618713 1114 8 0.35908190449511324 1114 9 0.3322496211815249 1114 10 0.24687619410762096 1114 11 0.35055181964837345 1114 12 0.2623287129797234 1109 1 0.34662949233631934 1109 2 0.3142656514746882 1109 3 0.3372575179210422 1109 4 0.10244793512460859 1109 5 0.17215922963460173 1109 6 0.2173610590585895 1109 7 0.28848408659087 1109 8 0.31383769863203526 1109 9 0.23035957960454567 1109 10 0.1699527180321212 1109 11 0.1952799476094711 1109 12 0.16636986468422776 1108 1 0.30249690678886054 1108 2 0.28959983817454216 1108 3 -0.34369299380367835 1108 4 0.025542881097130987 1108 5 0.10151760246957409 1108 6 0.21786722400975123 1108 7 0.25964182816914416 1108 8 0.2903651525310451 1108 9 0.23664397367706158 1108 10 0.17017528488808864 1108 11 0.20058048243848248 1108 12 0.17122621346606337 1111 1 0.1633419500727129 1111 2 0.10252283556755515 1111 3 0.11455082857672139 1111 4 0.01824234870204222 1111 5 0.06323594339051652 1111 6 0.12232249212484447 1111 7 0.10075872087031712 1111 8 0.14409874548312018 1111 9 0.0933416943262344 1111 10 0.03768411463109089 1111 11 0.07255899830651202 1111 12 0.004605588335358846 1110 1 0.170500897763052 1110 2 0.1230593683585326 1110 3 0.12854630824724467 1110 4 0.08750863006653731 1110 5 0.0888703587699311 1110 6 0.08260879885123984 1110 7 0.051992866002358085 1110 8 -0.039875791990368725 1110 9 0.04161663894908685 1110 10 -0.05253084546920661 1110 11 0.0816426460183024 1110 12 0.017019547440170868 1105 1 0.10984886124720461 1105 2 0.0543469096454281 1105 3 0.08678379907878409 1105 4 -0.020894798988297936 1105 5 0.019311048887924898 1105 6 0.08408830454982308 1105 7 0.016383983052215 1105 8 0.031036370779681022 1105 9 -4.110748252657004E-4 1105 10 -0.05463376822423527 1105 11 -0.0035238183605557083 1105 12 -0.057057427240856384 1104 1 -0.8265121866851146 1104 2 -0.5849301500677306 1104 3 -0.2029823187953319 1104 4 -0.1360623000540322 1104 5 -0.06800831939075636 1104 6 -0.024796410779451605 1104 7 0.14615241075050278 1104 8 0.2713235820641202 1104 9 0.156069934879433 1104 10 0.11212991201957578 1104 11 0.13784186048822278 1104 12 0.14245139572035015 1107 1 -0.1680865528701112 1107 2 -0.17724192685825457 1107 3 0.005039598092579922 1107 4 -0.06414015168587994 1107 5 -0.02244476344326074 1107 6 -0.049786674203808445 1107 7 -0.1163819755758837 1107 8 0.19984039284858568 1107 9 0.06716380535771549 1107 10 0.05091732818555528 1107 11 -0.16040396337456286 1107 12 0.09326300931172682 1106 1 0.048941446692698065 1106 2 0.057065593125958713 1106 3 0.13303530434829725 1106 4 0.13071562222927469 1106 5 0.11496487871453098 1106 6 0.011411847422834853 1106 7 0.161000257983396 1106 8 0.27215253145637774 1106 9 0.22748554615475314 1106 10 0.11242769775090977 1106 11 -0.044850380283229185 1106 12 0.13822765982549612 2103 1 -0.12447088569541265 2103 2 -0.06946036978867909 2103 3 -0.043253155893971496 2103 4 -0.0542625793886522 2103 5 0.014420758107711151 2103 6 -0.03327605962007851 2103 7 -0.07391239964548646 2103 8 0.10852177803810292 2103 9 0.06099453960298007 2103 10 0.07467400917115441 2103 11 -0.14112515912253798 2103 12 0.016124390081891704 2102 1 -0.15656362538455681 2102 2 -0.041370373729797905 2102 3 0.04096336935301892 2102 4 0.05519609985631391 2102 5 -0.030022838931113682 2102 6 0.09962941479707155 2102 7 0.032883389270480734 2102 8 0.140820493210704 2102 9 0.17955387103704368 2102 10 0.10672155768238412 2102 11 -0.030498151202536405 2102 12 -0.03485137453107967 2101 1 -0.036767316680457185 2101 2 -0.0679793591280955 2101 3 -0.30320441088209904 2101 4 -0.07477023834236363 2101 5 -0.09433891300707486 2101 6 -0.08761895598319569 2101 7 -0.1932176352527506 2101 8 -0.8691873169114999 2101 9 -0.14223890174785936 2101 10 -0.06774328761787984 2101 11 0.02548644592165772 2101 12 -0.19661548832726794 2107 1 -0.08937338852767951 2107 2 -0.2614075327878922 2107 3 -0.211050006029037 2107 4 -0.10497167789278805 2107 5 -0.1544015334930542 2107 6 -0.2624652340864344 2107 7 -0.15149078827445805 2107 8 -0.12057023230989472 2107 9 -0.08560494547842978 2107 10 -0.2601649230321357 2107 11 0.06578668041832714 2107 12 -0.12649878222543975 2106 1 0.03678522498205439 2106 2 -0.01268239564698348 2106 3 -0.002490013279683012 2106 4 -0.2605558846586753 2106 5 -0.09794111111093073 2106 6 -0.11807236512581198 2106 7 -0.10877871171493325 2106 8 0.011714240190315195 2106 9 -0.09479957372354164 2106 10 -0.11080842828817339 2106 11 0.09186870488757393 2106 12 -0.0641666795987561 2105 1 -0.14205065076103907 2105 2 -0.1398545616229967 2105 3 -0.27180862522880034 2105 4 -0.13243482816755403 2105 5 -0.4914356898567931 2105 6 -0.38836182961726706 2105 7 -0.351282784697581 2105 8 -0.25049114204153256 2105 9 -0.36146139283443546 2105 10 -0.41252792100923585 2105 11 -0.06481624216367265 2105 12 -0.016144096928748297 2104 1 -0.1979300184721744 2104 2 -0.32139306416195623 2104 3 -0.20822779125351332 2104 4 -0.26155852165514304 2104 5 -0.15460776813501198 2104 6 -0.21365402396795474 2104 7 -0.29158915888267956 2104 8 -0.19370442144857236 2104 9 -0.30177893203485695 2104 10 -0.3622858192781422 2104 11 -0.43335226111499026 2104 12 -0.10646666130161009 2111 1 -0.7000957378465209 2111 2 -0.5182056172315335 2111 3 -1.0697150394497932 2111 4 -0.5863051396996184 2111 5 -0.4680608586566457 2111 6 -0.4201855673611945 2111 7 -0.4457991246209829 2111 8 -0.3454160163041067 2111 9 -0.43041052413036596 2111 10 -0.3235025626723811 2111 11 -0.6306106475617099 2111 12 -0.25081907295567163 2110 1 -0.3857446656037702 2110 2 -0.42878583526616154 2110 3 -0.2960120610909307 2110 4 -0.5264119581669533 2110 5 -0.5486402906773868 2110 6 -0.4523972615776799 2110 7 -0.6152353651660931 2110 8 -0.4897602475111782 2110 9 -0.5703190779434308 2110 10 -0.39339657932896444 2110 11 -0.7876155225985961 2110 12 -0.3665384320485927 2109 1 -0.6371124020815984 2109 2 -0.6223533536557007 2109 3 -0.6083896480293625 2109 4 -0.39525724305934773 2109 5 -0.4008073236326233 2109 6 -0.4341478918165649 2109 7 -0.48249284081185806 2109 8 -0.5138290557509038 2109 9 -0.6331046505245794 2109 10 -0.3744021568266618 2109 11 -0.7353382135968047 2109 12 -0.6793646736401442 2108 1 -0.8979832364861231 2108 2 -0.8432938472885745 2108 3 -0.6437482701787403 2108 4 -0.6677745105602924 2108 5 -0.6839361088890463 2108 6 -0.5921362123793656 2108 7 -0.9999452933756388 2108 8 -0.7178294851841613 2108 9 -0.9069157197358031 2108 10 -0.6535946097487724 2108 11 -0.779209188559367 2108 12 -0.8628936340744886 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 55.0 15 2402.0 16 75223.0 17 168833.0 18 225828.0 19 301904.0 20 297788.0 21 317341.0 22 317028.0 23 289307.0 24 259026.0 25 259959.0 26 248705.0 27 248466.0 28 264442.0 29 292299.0 30 347514.0 31 483285.0 32 698347.0 33 2108683.0 34 4746275.0 35 4591215.0 36 632372.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.8918505542842 24.504874362617272 33.24431336975601 11.35896171334252 2 23.51033520205199 24.672646263627136 25.855002390794713 25.962016143526164 3 36.6962622968152 23.873975863365658 16.809007203357044 22.620754636462095 4 23.45178358292244 11.261076819992109 35.76848374245042 29.51865585463502 5 55.560200199146536 14.933620442170975 23.589665455831373 5.916513902851121 6 26.983901128398045 6.300141409990756 30.479771047275207 36.23618641433599 7 24.01797663372961 34.98766934456245 22.92735157059755 18.067002451110387 8 26.8376546375307 24.256615046689927 25.392244055412178 23.513486260367195 9 25.270441003669188 28.971384227927594 28.287470809336845 17.47070395906638 10 21.04681236007971 14.375630556458125 52.77263195903051 11.804925124431652 11 19.056354230484022 11.624275011080677 34.93693664006858 34.382434118366724 12 39.86922442417155 21.377268999090372 19.30348079702399 19.45002577971409 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 858.0 1 858.0 2 858.0 3 858.0 4 4184.0 5 7510.0 6 7510.0 7 7510.0 8 7510.0 9 7510.0 10 7510.0 11 7510.0 12 7510.0 13 19395.5 14 31281.0 15 31281.0 16 31281.0 17 31281.0 18 31281.0 19 31281.0 20 31281.0 21 58701.0 22 86121.0 23 86121.0 24 86121.0 25 86121.0 26 86121.0 27 86121.0 28 86121.0 29 182393.5 30 278666.0 31 278666.0 32 278666.0 33 278666.0 34 278666.0 35 278666.0 36 278666.0 37 278666.0 38 2433814.5 39 4588963.0 40 4588963.0 41 4588963.0 42 4588963.0 43 4588963.0 44 4588963.0 45 4588963.0 46 4939821.0 47 5290679.0 48 5290679.0 49 5290679.0 50 5290679.0 51 5290679.0 52 5290679.0 53 5290679.0 54 5966357.5 55 6642036.0 56 6642036.0 57 6642036.0 58 6642036.0 59 6642036.0 60 6642036.0 61 6642036.0 62 6642036.0 63 3421627.0 64 201218.0 65 201218.0 66 201218.0 67 201218.0 68 201218.0 69 201218.0 70 201218.0 71 120454.5 72 39691.0 73 39691.0 74 39691.0 75 39691.0 76 39691.0 77 39691.0 78 39691.0 79 23474.5 80 7258.0 81 7258.0 82 7258.0 83 7258.0 84 7258.0 85 7258.0 86 7258.0 87 7258.0 88 4327.5 89 1397.0 90 1397.0 91 1397.0 92 1397.0 93 1397.0 94 1397.0 95 1397.0 96 1008.0 97 619.0 98 619.0 99 619.0 100 619.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 5.821976646072201E-6 9 0.0 10 0.0 11 0.0 12 5.821976646072201E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 12 1.7176297E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 2.477085049072093 #Duplication Level Percentage of deduplicated Percentage of total 1 72.63926317616368 1.799336327892881 2 11.350924055195707 0.5623440854055611 3 4.333418236044999 0.32202736571650287 4 2.1633424808542063 0.2143513326138594 5 1.3474789603312731 0.16689099932879026 6 1.1925179877544674 0.17723810869296772 7 0.7233502906064349 0.12542601330722075 8 0.6198300969343526 0.12282974928647927 9 0.5704335920874622 0.12717112702035077 >10 4.052757398481248 2.0335785734061904 >50 0.4878756599131691 0.8299522315002656 >100 0.38832502644854694 1.9449356662830037 >500 0.060383330946480546 1.0724265828821866 >1k 0.0628104626032866 2.827585122262262 >5k 0.002586506515533585 0.4152025036202395 >10k+ 0.0047027391191519375 87.2587042107812 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGCTGTTGTTTG 1372621 7.99136740590827 No Hit GGATGGTGTTGC 1350859 7.864669550136447 No Hit TAGCTCGTAACT 1326596 7.723410930772796 No Hit GACTTTCCCTCG 1135795 6.612571964725575 No Hit TCTAGCGTAGTG 974225 5.67191519801969 No Hit CTGCTAACGCAA 888338 5.171883089818487 No Hit GGAGACAAGGGA 879771 5.122006215891585 No Hit ACGCGCAGATAC 792554 4.614230878751107 No Hit ATGTGCACGACT 775049 4.512317177561613 No Hit GCTGATGAGCTG 764724 4.452205268690918 No Hit ATCCCGAATTTG 756717 4.405588701685818 No Hit TTAGGGCTCGTA 724779 4.219646411563564 No Hit CAGTGCATATGC 670377 3.9029192380639435 No Hit GTTGGTCAATCT 649362 3.780570398846736 No Hit TCACGGGAGTTG 646354 3.7630578930953513 No Hit TCGAGGACTGCA 631377 3.6758621488671275 No Hit TAGGATTGCTCG 595750 3.4684425868975133 No Hit CTGCTCACGCAA 22167 0.12905575631348248 No Hit >>END_MODULE >>Adapter Content warn >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGGC 160 1.0913936E-11 6.0000005 1 AGCGTGG 65 0.0012619935 6.0000005 4 TTAGGCA 65 0.0012619935 6.0000005 1 TTGGCTG 65 0.0012619935 6.0000005 6 GGGTGCA 65 0.0012619935 6.0000005 1 TAGACCA 65 0.0012619935 6.0000005 2 ATAGCGT 160 1.0913936E-11 6.0000005 2 GTAGGTC 160 1.0913936E-11 6.0000005 1 AAGGTGT 80 6.539558E-5 6.0000005 3 GATCAAT 130 3.6470738E-9 6.0000005 4 GCTTGGT 65 0.0012619935 6.0000005 1 TCTAGGT 65 0.0012619935 6.0000005 1 TGTGCGC 80 6.539558E-5 6.0000005 2 TGGCCTA 160 1.0913936E-11 6.0000005 3 ATCCGGA 160 1.0913936E-11 6.0000005 1 GAGACAC 160 1.0913936E-11 6.0000005 2 GCCGAAT 80 6.539558E-5 6.0000005 3 ACGGCTC 235 0.0 6.0000005 3 TGTCTAT 65 0.0012619935 6.0000005 4 GTATGGC 160 1.0913936E-11 6.0000005 1 >>END_MODULE