FastQCFastQC Report
Thu 16 Apr 2015
000000000-AEB23 l01n02.33200000008792.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AEB23 l01n02.33200000008792.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17176297
Sequences flagged as poor quality0
Sequence length12
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTGTTGTTTG13726217.99136740590827No Hit
GGATGGTGTTGC13508597.864669550136447No Hit
TAGCTCGTAACT13265967.723410930772796No Hit
GACTTTCCCTCG11357956.612571964725575No Hit
TCTAGCGTAGTG9742255.67191519801969No Hit
CTGCTAACGCAA8883385.171883089818487No Hit
GGAGACAAGGGA8797715.122006215891585No Hit
ACGCGCAGATAC7925544.614230878751107No Hit
ATGTGCACGACT7750494.512317177561613No Hit
GCTGATGAGCTG7647244.452205268690918No Hit
ATCCCGAATTTG7567174.405588701685818No Hit
TTAGGGCTCGTA7247794.219646411563564No Hit
CAGTGCATATGC6703773.9029192380639435No Hit
GTTGGTCAATCT6493623.780570398846736No Hit
TCACGGGAGTTG6463543.7630578930953513No Hit
TCGAGGACTGCA6313773.6758621488671275No Hit
TAGGATTGCTCG5957503.4684425868975133No Hit
CTGCTCACGCAA221670.12905575631348248No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGC1601.0913936E-116.00000051
AGCGTGG650.00126199356.00000054
TTAGGCA650.00126199356.00000051
TTGGCTG650.00126199356.00000056
GGGTGCA650.00126199356.00000051
TAGACCA650.00126199356.00000052
ATAGCGT1601.0913936E-116.00000052
GTAGGTC1601.0913936E-116.00000051
AAGGTGT806.539558E-56.00000053
GATCAAT1303.6470738E-96.00000054
GCTTGGT650.00126199356.00000051
TCTAGGT650.00126199356.00000051
TGTGCGC806.539558E-56.00000052
TGGCCTA1601.0913936E-116.00000053
ATCCGGA1601.0913936E-116.00000051
GAGACAC1601.0913936E-116.00000052
GCCGAAT806.539558E-56.00000053
ACGGCTC2350.06.00000053
TGTCTAT650.00126199356.00000054
GTATGGC1601.0913936E-116.00000051
Produced by FastQC (version 0.11.2)