##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AEB23 l01n01.33100000008795.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17176297 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.025404195095135 33.0 32.0 33.0 32.0 34.0 2 32.66085582940258 33.0 32.0 34.0 32.0 34.0 3 32.38938305503218 33.0 32.0 34.0 32.0 34.0 4 32.418539222976875 33.0 32.0 34.0 32.0 34.0 5 32.52658684232114 33.0 32.0 34.0 32.0 34.0 6 33.99553011921021 34.0 33.0 37.0 32.0 37.0 7 36.03362861040421 37.0 37.0 37.0 35.0 37.0 8 36.07512329345493 37.0 37.0 37.0 33.0 37.0 9 35.835554951105 37.0 37.0 37.0 33.0 37.0 10-11 34.2716654526875 35.0 33.0 36.0 32.0 37.0 12-13 35.26239593435069 35.5 35.0 36.5 34.0 37.5 14-15 36.68548500296659 38.0 37.5 38.0 34.0 38.0 16-17 36.527865639491445 38.0 37.0 38.0 34.0 38.0 18-19 36.34137681131154 38.0 37.0 38.0 33.0 38.0 20-21 36.72918403192493 38.0 37.5 38.0 34.5 38.0 22-23 36.69216869037605 38.5 37.0 38.5 33.5 38.5 24-25 37.11749607613329 39.0 37.5 39.0 34.5 39.0 26-27 37.581099814471074 39.0 38.0 39.0 36.5 39.0 28-29 37.18369136840147 38.0 37.5 39.0 35.0 39.0 30-31 36.89916400490746 38.5 37.5 39.0 33.5 39.0 32-33 37.011334748112475 38.5 37.5 39.0 34.5 39.0 34-35 37.20562543253648 38.0 37.5 39.0 35.0 39.0 36-37 36.399709902547684 38.5 37.0 39.0 33.0 39.0 38-39 37.15895670062063 39.0 37.5 39.0 33.5 39.0 40-41 37.08063132001036 39.0 37.5 39.0 34.0 39.0 42-43 36.92728039693305 39.0 38.0 39.0 33.5 39.0 44-45 36.47389102552197 39.0 37.0 39.0 32.5 39.0 46-47 36.518507394230554 38.0 37.0 39.0 32.5 39.0 48-49 36.362667779906225 38.0 37.0 39.0 32.5 39.0 50-51 36.67232960631736 38.0 37.5 39.0 33.5 39.0 52-53 36.66514030934607 38.5 37.5 39.0 33.5 39.0 54-55 36.187912010371036 38.0 37.0 39.0 32.5 39.0 56-57 36.37287393784586 38.0 37.0 39.0 32.5 39.0 58-59 35.76586102930102 38.0 37.0 39.0 30.0 39.0 60-61 36.341514209960394 38.5 37.0 39.0 31.5 39.0 62-63 36.05075899071843 38.5 37.0 39.0 31.0 39.0 64-65 35.903749801252275 38.0 37.0 39.0 26.0 39.0 66-67 35.9798856237756 38.5 37.0 39.0 30.5 39.0 68-69 36.50250874795656 38.0 37.0 39.0 33.0 39.0 70-71 35.89938995582109 38.0 36.5 39.0 26.0 39.0 72-73 35.82858359982946 38.0 36.5 39.0 26.0 39.0 74-75 36.46041172320204 39.0 37.0 39.0 32.0 39.0 76-77 36.04548468741545 38.0 36.5 39.0 26.0 39.0 78-79 36.08755551327506 38.5 37.0 39.0 31.0 39.0 80-81 36.68134313234104 39.0 37.5 39.0 33.0 39.0 82-83 35.98109898192841 38.0 36.5 39.0 26.0 39.0 84-85 36.12301167125836 38.0 36.5 38.5 31.5 39.0 86-87 36.793940742873744 39.0 37.0 39.0 33.0 39.0 88-89 35.8455913984254 38.5 37.0 39.0 25.0 39.0 90-91 35.908938754377616 38.0 37.0 39.0 30.0 39.0 92-93 36.11989461407194 39.0 37.0 39.0 30.0 39.0 94-95 35.798252527887705 38.5 37.0 39.0 24.5 39.0 96-97 35.98272753434573 39.0 37.0 39.0 30.0 39.0 98-99 36.23855680301756 38.0 37.0 39.0 31.5 39.0 100-101 35.75245540409554 38.5 36.5 39.0 25.0 39.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14204044380351633 1101 2 0.2725538579014426 1101 3 0.2865432056919346 1101 4 0.2915919406019185 1101 5 0.3170505656189704 1101 6 0.45275029861890204 1101 7 0.23569902193803927 1101 8 0.2405194680616347 1101 9 0.3290746378258831 1101 10-11 0.3803688043077642 1101 12-13 0.4317773806628509 1101 14-15 0.45626566874089747 1101 16-17 0.5512230523550556 1101 18-19 0.6023034892306214 1101 20-21 0.5016667883944876 1101 22-23 0.4267030913408689 1101 24-25 0.4417263290036857 1101 26-27 0.41794727070337245 1101 28-29 0.4282118939641961 1101 30-31 0.7056216487666447 1101 32-33 0.4783456065891656 1101 34-35 0.49059026756022917 1101 36-37 1.0278521549985697 1101 38-39 0.8478897726147281 1101 40-41 0.7189492038793119 1101 42-43 0.5314663076287189 1101 44-45 0.6964016301881344 1101 46-47 0.4671836741843691 1101 48-49 0.6507189365186079 1101 50-51 0.41888672354761525 1101 52-53 0.5141723162224139 1101 54-55 0.46185198352738865 1101 56-57 0.3089441836098672 1101 58-59 0.24921871855551103 1101 60-61 0.8164386041526654 1101 62-63 0.7029988291669298 1101 64-65 0.4725380269278432 1101 66-67 0.4965313179901756 1101 68-69 0.48453873083710164 1101 70-71 0.3641200807765017 1101 72-73 0.3821663071832546 1101 74-75 0.46099246463377597 1101 76-77 0.40385370797458364 1101 78-79 0.4412165740980356 1101 80-81 0.4106351935370398 1101 82-83 0.29588893814015194 1101 84-85 0.3764801569266396 1101 86-87 0.4668599556419437 1101 88-89 0.4667783731017181 1101 90-91 0.3554644587091502 1101 92-93 0.4734282480754075 1101 94-95 0.30587060317395043 1101 96-97 0.4617343703562682 1101 98-99 0.43047453108720646 1101 100-101 0.4814247788106627 1102 1 0.28406287338569314 1102 2 0.23230891671084208 1102 3 0.3417147722804472 1102 4 0.35243542074362466 1102 5 0.3313883768369834 1102 6 0.39208391817440713 1102 7 0.22854255562852188 1102 8 0.2079956027721721 1102 9 0.22212766816946328 1102 10-11 0.32728854886791936 1102 12-13 0.32622531554000744 1102 14-15 0.3990062085505315 1102 16-17 0.47341465292091556 1102 18-19 0.5969419528100843 1102 20-21 0.3719535930619813 1102 22-23 0.4904908201742515 1102 24-25 0.41147248773175704 1102 26-27 0.4220201409751638 1102 28-29 0.40365324139289527 1102 30-31 0.6123679493170116 1102 32-33 0.531869225485508 1102 34-35 0.5281557099397034 1102 36-37 1.0620974758735144 1102 38-39 0.8503721889967935 1102 40-41 0.8033134302340201 1102 42-43 0.5362246069260763 1102 44-45 0.7344308975028895 1102 46-47 0.5591184997968668 1102 48-49 0.7686956100863185 1102 50-51 0.5428702503115019 1102 52-53 0.49356578929701556 1102 54-55 0.5437198392144253 1102 56-57 0.42830013027331404 1102 58-59 0.40478331419937064 1102 60-61 0.8523002990461848 1102 62-63 0.6686238802098927 1102 64-65 0.5109066453459761 1102 66-67 0.5561379361902539 1102 68-69 0.5414006892134324 1102 70-71 0.38590672367551804 1102 72-73 0.37389773206382415 1102 74-75 0.43052486267438894 1102 76-77 0.4162338675172066 1102 78-79 0.5171041804485839 1102 80-81 0.3195748522783006 1102 82-83 0.3801157873812784 1102 84-85 0.3748510710759376 1102 86-87 0.5232188109182587 1102 88-89 0.40164154539422725 1102 90-91 0.35377747142594984 1102 92-93 0.4756880338481295 1102 94-95 0.3265587087725734 1102 96-97 0.46576050109833034 1102 98-99 0.3947722734390453 1102 100-101 0.42991350045929977 1103 1 0.27625547751863877 1103 2 0.2510329300726042 1103 3 0.29548618362703394 1103 4 0.25937889967800487 1103 5 0.3428300188200879 1103 6 0.42796476191971067 1103 7 0.2634910138183528 1103 8 0.2501496976754751 1103 9 0.35513550797927707 1103 10-11 0.35820866148229413 1103 12-13 0.3808997287155407 1103 14-15 0.41263164418601406 1103 16-17 0.5121117954350112 1103 18-19 0.5625794238358566 1103 20-21 0.41805115678495497 1103 22-23 0.41028337106243384 1103 24-25 0.3467647108427059 1103 26-27 0.40448479847005814 1103 28-29 0.18993636387374835 1103 30-31 0.6662496113981646 1103 32-33 0.4388452138281096 1103 34-35 0.47715668500611486 1103 36-37 0.9561903780424075 1103 38-39 0.7855906432819424 1103 40-41 0.7319588535635475 1103 42-43 0.40734750105971074 1103 44-45 0.6435581205089349 1103 46-47 0.4748306972838918 1103 48-49 0.6997198137098835 1103 50-51 0.4545493686215636 1103 52-53 0.5492979148763908 1103 54-55 0.498980489819985 1103 56-57 0.37330923599962773 1103 58-59 0.3060408667281109 1103 60-61 0.8630681116210397 1103 62-63 0.7375877619213966 1103 64-65 0.4579582654665444 1103 66-67 0.48173620739610357 1103 68-69 0.4788435033018956 1103 70-71 0.36460545768119346 1103 72-73 0.3192291826094191 1103 74-75 0.4217766015930593 1103 76-77 0.44922137331415257 1103 78-79 0.49216903587683447 1103 80-81 0.26957440253682563 1103 82-83 0.4614108692545358 1103 84-85 0.5015636629109324 1103 86-87 0.5786657922608001 1103 88-89 0.4273362211185585 1103 90-91 0.43119910934473893 1103 92-93 0.3676963512768623 1103 94-95 0.21434253710086182 1103 96-97 0.3512904306178797 1103 98-99 -0.6831394200111731 1103 100-101 -0.6534450954606541 2112 1 0.33304377640267546 2112 2 0.2263202823703523 2112 3 0.3100005663311549 2112 4 0.42148019937906156 2112 5 0.2755674758334905 2112 6 0.4611321485186153 2112 7 0.3225266629698922 2112 8 0.2168945746179034 2112 9 0.3577864582731394 2112 10-11 0.2842523372173318 2112 12-13 0.3718997336236569 2112 14-15 0.4996301288215079 2112 16-17 0.5518094632469612 2112 18-19 0.6101203892705485 2112 20-21 0.5213077614537127 2112 22-23 0.4733915074393735 2112 24-25 0.25425070917358994 2112 26-27 0.4060097070181783 2112 28-29 0.3721434439257578 2112 30-31 0.7569337742924276 2112 32-33 0.5886177188625155 2112 34-35 0.5360707440010657 2112 36-37 1.0238066922646425 2112 38-39 0.8356461983130217 2112 40-41 0.6923154768037136 2112 42-43 0.4398552655392436 2112 44-45 0.7158237563796774 2112 46-47 0.4815791378771834 2112 48-49 0.6778348929184332 2112 50-51 0.47301568544225603 2112 52-53 0.5726168996930809 2112 54-55 0.5504484774391827 2112 56-57 0.41621076253088063 2112 58-59 0.3658319214311305 2112 60-61 0.8916688146224274 2112 62-63 0.8237811692498056 2112 64-65 0.4798417735575171 2112 66-67 0.4549005227570859 2112 68-69 0.4655086374287194 2112 70-71 0.31880171723585704 2112 72-73 0.35309611643535277 2112 74-75 0.43544386221590514 2112 76-77 0.5080176362447588 2112 78-79 0.5085016743204207 2112 80-81 0.1909457235705858 2112 82-83 0.347447950868343 2112 84-85 0.3756317617122491 2112 86-87 0.3325037413404388 2112 88-89 -1.747363851763609 2112 90-91 -0.23892255341750257 2112 92-93 -0.6734838822501672 2112 94-95 0.2388460453983825 2112 96-97 0.4298713179394795 2112 98-99 0.0550742047237236 2112 100-101 0.30048361254229405 1113 1 0.3242310248377862 1113 2 0.21227104905467087 1113 3 0.2911821516109683 1113 4 0.32514465343628984 1113 5 0.30976369534674575 1113 6 0.48622441791072646 1113 7 0.3472583593293024 1113 8 0.3133540594465316 1113 9 0.4357519898547011 1113 10-11 0.2816432632806922 1113 12-13 0.2985445393305852 1113 14-15 0.30142606285778584 1113 16-17 0.4356614355758879 1113 18-19 0.0770190510323232 1113 20-21 0.33103101463484563 1113 22-23 0.30996191625469294 1113 24-25 0.39532826305188706 1113 26-27 0.38157569283296056 1113 28-29 0.4519308422803263 1113 30-31 0.7226481995265104 1113 32-33 0.5073576664012407 1113 34-35 0.5056228838754322 1113 36-37 1.0458855558121911 1113 38-39 0.8230788804770626 1113 40-41 0.7141342006262974 1113 42-43 0.4316748244233537 1113 44-45 0.6944640283897883 1113 46-47 0.44792911382012335 1113 48-49 0.7291994599574494 1113 50-51 0.5343459682121008 1113 52-53 0.5187741753280051 1113 54-55 0.49674845488129193 1113 56-57 0.3704911118355909 1113 58-59 0.4142567728217941 1113 60-61 0.9400641932311302 1113 62-63 0.7865706263182091 1113 64-65 0.44540161735354644 1113 66-67 0.42621025152735825 1113 68-69 0.48594844739358933 1113 70-71 0.4308719062659918 1113 72-73 0.3983978708946623 1113 74-75 0.37744385447278717 1113 76-77 0.5127162990559739 1113 78-79 0.4808020729442646 1113 80-81 0.25031661825981644 1113 82-83 0.35171139495444237 1113 84-85 0.4350641846555021 1113 86-87 0.43088861221746555 1113 88-89 0.3496096461453817 1113 90-91 0.28166561957653613 1113 92-93 0.5642851313513617 1113 94-95 0.4054301714459356 1113 96-97 0.41657680727154656 1113 98-99 -2.1938248626440782E-4 1113 100-101 0.23056253526369375 2113 1 0.24211692827537235 2113 2 0.1355379788063118 2113 3 0.2609079294320651 2113 4 0.2616065482592589 2113 5 0.3249174133437549 2113 6 0.43999542312359097 2113 7 0.28619746303513693 2113 8 0.27903527436623676 2113 9 0.4498831330914612 2113 10-11 0.27224846535040825 2113 12-13 0.2920016880324994 2113 14-15 0.3940167337510445 2113 16-17 0.3693024888762224 2113 18-19 -0.5590658965227178 2113 20-21 0.285092776963765 2113 22-23 0.5341056985586405 2113 24-25 0.5510085234393216 2113 26-27 0.3658917404884505 2113 28-29 0.37104780231694434 2113 30-31 0.7289323322633336 2113 32-33 0.5160726011728372 2113 34-35 0.44827977143104647 2113 36-37 1.0289229538128808 2113 38-39 0.7998367404711288 2113 40-41 0.6897853147427142 2113 42-43 0.4457474333224454 2113 44-45 0.7043453668194957 2113 46-47 0.42625202742667057 2113 48-49 0.7414080665806111 2113 50-51 0.510687266661769 2113 52-53 0.49961986731382524 2113 54-55 0.5453955865764328 2113 56-57 0.38290273294079924 2113 58-59 0.4518261566781163 2113 60-61 0.8523367626733886 2113 62-63 0.7446388155553834 2113 64-65 0.47668844854255354 2113 66-67 0.5400784215220824 2113 68-69 0.5027780248132387 2113 70-71 0.3522842745049246 2113 72-73 0.3802839915746574 2113 74-75 0.39085705159162387 2113 76-77 0.4901354479382789 2113 78-79 0.4825937396626685 2113 80-81 0.2815354188812762 2113 82-83 0.3876051548780808 2113 84-85 0.440688829953622 2113 86-87 0.3823164446635374 2113 88-89 0.34577058375072056 2113 90-91 0.26022831402142543 2113 92-93 0.5201040564975727 2113 94-95 0.3344480243366945 2113 96-97 0.38159907725524533 2113 98-99 0.2990254451042773 2113 100-101 0.503701148483259 1112 1 0.1709361947588306 1112 2 0.1496761518284515 1112 3 0.14121711903570144 1112 4 0.109246609820012 1112 5 0.21157635751424664 1112 6 0.2246890053534969 1112 7 0.1276754004753613 1112 8 0.24989638725105578 1112 9 0.3896477791314368 1112 10-11 0.3164257159548498 1112 12-13 0.3341378948368643 1112 14-15 0.4195276915130819 1112 16-17 0.44708394680609587 1112 18-19 0.6053105206086187 1112 20-21 0.384102448876547 1112 22-23 0.45683961965525555 1112 24-25 0.47418487971729206 1112 26-27 0.2915179381629258 1112 28-29 0.24025697941723223 1112 30-31 0.6171724535918273 1112 32-33 0.4030788710705693 1112 34-35 0.42454838965913666 1112 36-37 0.9487931829558605 1112 38-39 0.7542452522147656 1112 40-41 0.5694217668252861 1112 42-43 0.38215126200656613 1112 44-45 0.5734131470210713 1112 46-47 0.4109005815504787 1112 48-49 0.6289846033870816 1112 50-51 0.3904534423735342 1112 52-53 0.47085556948782425 1112 54-55 0.4333267206353142 1112 56-57 0.27241290034601917 1112 58-59 0.21686948267485917 1112 60-61 0.7667854371661491 1112 62-63 0.6864565637941666 1112 64-65 0.408088435322135 1112 66-67 0.5009177308732831 1112 68-69 0.459145365173697 1112 70-71 0.3478219673711891 1112 72-73 0.3462116427776678 1112 74-75 0.3389321832621306 1112 76-77 0.2887754429451448 1112 78-79 0.33365052394312755 1112 80-81 0.2535357509655185 1112 82-83 0.2680036869570728 1112 84-85 0.28383713107741215 1112 86-87 0.3142487944680852 1112 88-89 0.28542108063632554 1112 90-91 0.20496432832183586 1112 92-93 0.33210660443268125 1112 94-95 0.2004600131398675 1112 96-97 0.3028354911273752 1112 98-99 0.11897713398207088 1112 100-101 0.2733921862312201 2114 1 0.3289627174843446 2114 2 0.23637691223656532 2114 3 0.29491852497620386 2114 4 0.19867754016205907 2114 5 0.34618521334925134 2114 6 0.4164046819180811 2114 7 0.2933595163082714 2114 8 0.3099528893516492 2114 9 0.3213701113090579 2114 10-11 0.389902255991565 2114 12-13 0.3976322090091955 2114 14-15 0.4903716454339673 2114 16-17 0.41238620612976007 2114 18-19 0.5242226127910072 2114 20-21 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-0.6010013268945471 2109 24-25 -0.5670627791606719 2109 26-27 -0.4845042157152548 2109 28-29 -0.21783992980405031 2109 30-31 -0.200607930326548 2109 32-33 -0.11374745320896551 2109 34-35 -0.4225917112644453 2109 36-37 0.17354835314588968 2109 38-39 -0.00790825186741273 2109 40-41 -0.43068810070742103 2109 42-43 -0.5339558187768247 2109 44-45 -1.7177668787582405 2109 46-47 -0.5490331183201533 2109 48-49 -0.7274869604855709 2109 50-51 -0.7357867578908355 2109 52-53 -0.3340755907626658 2109 54-55 -0.3473563524566359 2109 56-57 -0.3929240305991897 2109 58-59 -0.7082995648836103 2109 60-61 -0.23550110854673534 2109 62-63 0.07478371651459526 2109 64-65 -0.5440422627159833 2109 66-67 -0.3459903882425408 2109 68-69 -0.8353024587952333 2109 70-71 -0.3904285494332669 2109 72-73 -0.41263050849187977 2109 74-75 -0.6138531874572664 2109 76-77 -0.8638493159760969 2109 78-79 -0.5716739277646141 2109 80-81 -0.5363154542187729 2109 82-83 -0.9003167895019359 2109 84-85 -0.5515303484023022 2109 86-87 -0.3479398128884199 2109 88-89 -0.4401930734905548 2109 90-91 -0.09678708152333826 2109 92-93 -0.5950647937306783 2109 94-95 -0.3170272792621489 2109 96-97 -0.6047550356584424 2109 98-99 -0.447702083358692 2109 100-101 -0.7026308377794805 2108 1 -0.8654833992051749 2108 2 -0.5209854070291726 2108 3 -0.6472678161254528 2108 4 -1.0615577577761606 2108 5 -0.9615506961346831 2108 6 -1.1497106567245154 2108 7 -0.630688280913354 2108 8 -0.755584063158004 2108 9 -0.8918979249339003 2108 10-11 -1.0090084872540999 2108 12-13 -0.6998500130894811 2108 14-15 -1.2113027904307216 2108 16-17 -1.3267189407568267 2108 18-19 -0.7324384878862276 2108 20-21 -0.2909609546240546 2108 22-23 -0.6611967942669565 2108 24-25 -0.7475229884684751 2108 26-27 -0.7604768042173191 2108 28-29 -0.6096462228634678 2108 30-31 -0.768861914987049 2108 32-33 -0.6418150980699195 2108 34-35 -0.9462023524249474 2108 36-37 -0.2580925027730885 2108 38-39 -0.5284522721386153 2108 40-41 -1.1104115733402509 2108 42-43 -0.7853276384278374 2108 44-45 -0.39915992130933375 2108 46-47 -1.050590871003564 2108 48-49 -0.863640432934524 2108 50-51 -0.9751833213996264 2108 52-53 -1.056212720953127 2108 54-55 -1.37666415278823 2108 56-57 -1.1807599460888625 2108 58-59 -0.6569419976013862 2108 60-61 -0.5429367181287006 2108 62-63 -0.3845447085577405 2108 64-65 -0.7061612242869302 2108 66-67 -0.7477977661711321 2108 68-69 -1.1140623117891124 2108 70-71 -0.5689602319158098 2108 72-73 -1.208788650533748 2108 74-75 -0.9410719323036005 2108 76-77 -0.8969682330142703 2108 78-79 -0.6496456700969517 2108 80-81 -0.9547716275499241 2108 82-83 -0.7655264563398845 2108 84-85 -0.6866639036972586 2108 86-87 -1.035086958250595 2108 88-89 -0.5003746139079084 2108 90-91 -0.6925402715771369 2108 92-93 -0.9369024746368453 2108 94-95 -0.9787614748415336 2108 96-97 -0.8204550864032356 2108 98-99 -0.6819491853307937 2108 100-101 -0.4719417351844868 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 8.0 15 43.0 16 132.0 17 213.0 18 248.0 19 282.0 20 542.0 21 1367.0 22 4640.0 23 15178.0 24 38071.0 25 72004.0 26 110464.0 27 158916.0 28 207043.0 29 261468.0 30 355693.0 31 440825.0 32 555051.0 33 746304.0 34 997318.0 35 1286344.0 36 2041569.0 37 6054099.0 38 3828473.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.93171607361005 52.54746119026703 1.560330494983872 0.9604922411390535 2 0.897405302202215 1.5916934831762632 5.6272781030742545 91.88362311154727 3 1.0012868314980814 55.3482918931828 42.18748662764739 1.4629346476717304 4 11.393392883227392 8.894059062905118 22.300534276974833 57.41201377689266 5 20.880653146600807 1.2308415486760622 16.36798665044043 61.5205186542827 6 1.0189041328290958 52.392183251139635 39.4688040152077 7.120108600823564 7 1.4745786009638748 35.51397603336738 56.86697778921731 6.144467576451432 8 76.59281139542541 1.262926535499854 1.8288518083293392 20.315410260745388 9 57.80026975546592 1.4214355981385278 20.852340874170956 19.925953772224595 10-11 38.47659364530085 59.06221521437362 1.3715063264218126 1.0896848139037185 12-13 38.306251341601744 2.273394550641503 45.83003251515737 13.590321592599382 14-15 50.21660664111711 30.964884340320854 14.115117478464652 4.70339154009738 16-17 34.46146454034883 26.247956704521354 35.29535498833072 3.9952237667990955 18-19 37.78031544809441 6.139248353101031 42.644818689523674 13.435617509280886 20-21 4.070193360070567 31.703131355961066 26.442821173853716 37.78385411011465 22-23 26.427704411492186 13.360548551297175 26.591444593674645 33.620302443536 24-25 45.39492417952484 23.12244891899575 19.13917475926272 12.343452142216684 26-27 11.810147437483177 83.56449297540675 1.9697930234904533 2.6555665636196206 28-29 37.81551693010432 9.98960369630311 20.159263664339292 32.03561570925328 30-31 40.482808954689126 23.862101941996 29.347818682921005 6.307270420393872 32-33 31.05339002929444 2.742608025466723 48.948603415509176 17.255398529729664 34-35 50.57135132211559 28.69509068223494 16.409508987880216 4.324049007769253 36-37 23.145192470763636 22.718930628644813 16.02898750528126 38.1068893953103 38-39 14.19835427857355 30.076185804192836 45.31279064399038 10.412669273243237 40-41 12.54869719590899 39.562788184205246 18.446490532854664 29.4420240870311 42-43 14.132018676668201 14.3789257952398 38.48732063727123 33.001734890820764 44-45 25.37688420268932 20.58267564888986 32.30728660548895 21.73315354293187 46-47 34.37767465245856 27.184075240431625 17.36515152247309 21.073098584636725 48-49 28.844625241400983 41.366081408583 15.155245627157004 14.634047722859005 50-51 51.44525912657425 15.89633958937715 16.324807960644836 16.333593323403758 52-53 27.194924493911582 21.773226208186784 18.909378430053927 32.12247086784771 54-55 31.052458513031066 29.419836534032918 9.573445894653545 29.954259058282467 56-57 25.128137339497563 19.889318984179187 37.789198684675746 17.193344991647503 58-59 24.726674206902686 37.34720586165924 18.78884895853862 19.137270972899458 60-61 26.69320692347134 40.65302899687867 9.424604150708387 23.229159928941613 62-63 19.537223302554676 30.824676587741816 28.565959240225062 21.072140869478446 64-65 23.042385096158966 24.399656689681134 40.13252099681323 12.425437217346673 66-67 38.93553133134575 21.752895865738697 34.096511023301474 5.215061779614081 68-69 32.18790697436123 38.56978602547452 11.077608869944436 18.164698130219804 70-71 8.904008820993257 24.559007101472456 33.4765491071795 33.060434970354784 72-73 19.495698054126567 24.74997666842859 20.238585185153703 35.51574009229114 74-75 39.81421606764252 21.278058361473374 30.8802502658169 8.02747530506721 76-77 17.395385553573785 29.880801137777286 18.385418533034755 34.33839477561417 78-79 32.56150321573969 27.751115388840798 33.75170445643785 5.9356769389816675 80-81 49.75458621843812 18.01165291913618 25.64766724748646 6.586093614939238 82-83 34.560994142101755 17.364243294116307 33.5368764291861 14.537886134595832 84-85 29.94288291591604 43.38987326546577 19.149802777630125 7.5174410409880545 86-87 20.391938960999568 61.24363999993713 6.234469513423062 12.129951525640246 88-89 8.847596778281138 31.848960226991885 20.776017671329274 38.5274253233977 90-91 33.24388545447252 36.97094897695353 23.992074659631236 5.793090908942713 92-93 26.722762965811636 48.15808227344003 18.926498503329864 6.192656257418472 94-95 17.254903661714746 51.946930121201326 9.839321013138047 20.95884520394588 96-97 22.400008569949623 28.426950814835116 15.228139685754153 33.944900929461106 98-99 40.382270415511286 16.483075964600403 38.403226213520476 4.731427406367839 100-101 23.351791813794083 61.94215382809676 6.265017607258934 8.44103675085022 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.0 2 2.0 3 1.5 4 2.0 5 3.0 6 3.0 7 5.0 8 5.0 9 4.0 10 11.0 11 12.5 12 9.0 13 24.0 14 42.5 15 42.5 16 53.0 17 75.5 18 120.0 19 181.5 20 253.5 21 277.0 22 259.5 23 712.5 24 950.0 25 1439.5 26 1956.5 27 3656.5 28 4844.5 29 12005.5 30 16697.5 31 11657.0 32 37643.5 33 157654.5 34 172113.5 35 81472.0 36 84101.5 37 206779.5 38 260424.0 39 160711.5 40 131512.5 41 190439.5 42 296606.5 43 1127787.5 44 1564841.0 45 906022.0 46 466409.0 47 366339.0 48 595514.0 49 1794058.5 50 3252143.0 51 2434511.0 52 1003560.0 53 823641.0 54 507130.5 55 190528.0 56 118472.0 57 69829.5 58 35414.5 59 22672.5 60 19864.5 61 18830.0 62 14022.5 63 5100.5 64 2194.0 65 1018.5 66 531.5 67 314.0 68 140.5 69 109.5 70 128.0 71 85.0 72 42.0 73 29.5 74 23.0 75 16.0 76 9.0 77 3.5 78 4.5 79 4.5 80 3.0 81 2.0 82 1.0 83 3.0 84 3.5 85 3.0 86 3.5 87 2.0 88 0.5 89 1.0 90 2.5 91 2.5 92 3.5 93 5.5 94 6.0 95 3.0 96 3.0 97 4.5 98 8.0 99 17.5 100 60.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 5.821976646072201E-6 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.9109883230361004E-6 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 2.9109883230361004E-6 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 2.9109883230361004E-6 94-95 0.0 96-97 0.0 98-99 2.9109883230361004E-6 100-101 2.9109883230361004E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7176297E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 2.936244169733579 #Duplication Level Percentage of deduplicated Percentage of total 1 76.75499612132108 2.2537140985915247 2 9.394013323318688 0.5516623370198812 3 2.9183653685300746 0.25707099895496455 4 1.8365528773028197 0.21570270713551334 5 1.0326036562791514 0.15159882326976176 6 0.6970353680487779 0.1227999621318794 7 0.6379631599988653 0.1311250926336035 8 0.5937897808079684 0.13948094255558216 9 0.39967797815603007 0.10561969198183935 >10 3.545771060411182 2.5126012100994966 >50 1.389590501216437 2.7859344744989 >100 0.5438439831386918 3.2415079009184735 >500 0.10603169973182486 2.1671326190665905 >1k 0.11136549012525893 7.177327306195667 >5k 0.01622230427059061 3.29345381490015 >10k+ 0.02217732734267057 74.89326802004625 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG 2181870 12.702796184765553 No Hit ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC 1628437 9.480722183599877 No Hit GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGAATAA 1304643 7.595601077461573 No Hit GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCAAAG 932404 5.428434312704304 No Hit ACACCATGGGAGTCGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC 691584 4.026385896797197 No Hit ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG 667108 3.8838871964079336 No Hit ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAGTAGCTACC 416817 2.4266988396858764 No Hit GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCATAA 245042 1.426628801306824 No Hit GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACTGTATGTG 236859 1.3789875664120153 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGCTTAACCTTCGGGAG 230935 1.3444981767606836 No Hit GCTGTTACCGATTGATTATTTTGATGAGTATTGGGGAGTTTAATAATAGG 219229 1.2763461181417624 No Hit GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTTTCAGCC 208992 1.2167465432159212 No Hit ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG 187367 1.0908462982446099 No Hit GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTCAGCAATGA 179116 1.0428091689378682 No Hit GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGACGCAAGTTT 173022 1.0073300432567043 No Hit ACACCATGGGAATTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG 159581 0.9290768551568478 No Hit GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGGACAGGAC 111357 0.648317853376662 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTTCGGGG 111015 0.6463267373637054 No Hit GCTGTCCGGGACTGAGCTGTTTCGAGAAGAGTGAGGACTGCTGCAGCGAG 101429 0.5905172692344572 No Hit ACACCATGGGAGTCTATTGCACCAGAAGTAGGTAGCCTAACGAAAGAGGG 84849 0.49398889644258015 No Hit GTTGTTTCCGATGATGGTGGAATGCAGGTGATCGGACTGGCCTCGGCCGG 74537 0.43395267326828363 No Hit GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTTGCAAAGCT 72128 0.4199275315278957 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTCGGGAG 70048 0.4078178201040655 No Hit GCTGTTACCGATTGAATGGTTCAATGAGTAGTAAGGAGTTTGAGGCAGCA 67624 0.3937053487139865 No Hit GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACTAGCAGACGTTTT 65627 0.38207886135178026 No Hit ACACCATGGGAGTGGGGGATACCAGAAGTGGGTAGGCTAACCGCAAGGAG 65332 0.380361378241189 No Hit ACACCATGGGAGTGGAGGATACCAGAAGTAGCTAGACTAACCGCAAGGGG 64427 0.3750924893764937 No Hit GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACGGCATGTG 64422 0.3750633794932633 No Hit GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGTGTCACCGCT 56443 0.3286098278342532 No Hit ACACCATGGGAGTGGGTTGTACCAGAAGTAGTTAGTCTAACCCTTCGGGG 55708 0.32433067499939017 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTAGGGGG 54937 0.3198419310052685 No Hit GCTGCCCGGAACTGAGCCATTTCGAGAAAAATCGGGACTGACTGCTTGTG 49432 0.287791949568641 No Hit GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCCTTACCGC 44846 0.2610923646697539 No Hit GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGGGCGTAAGCTC 40158 0.2337989381529674 No Hit ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCCTAACCTTCGGGAG 39187 0.2281457988296313 No Hit GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACCGTATGTG 38010 0.22129333231720436 No Hit GCTGTCCGGGACTGAGCTGTTTCGAGAAGACTGAGGACTGCTGCAGCGAG 37971 0.22106627522800754 No Hit TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTGCTGA 37813 0.2201464029179281 No Hit GCACCTACCGATTGAATGACTCGGTGAAAAATTGGGACCGTGAAACTGTT 37470 0.21814946492832535 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAG 36986 0.21533162823162644 No Hit GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTCCGTGCC 34534 0.2010561414954574 No Hit GCTATCCGGGACTGAGTTGTTTCGAGAGGAGTGGAGACTGTTGTTTTGGG 32845 0.19122282294024143 No Hit GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACCGATGTGCGGAG 32683 0.1902796627235777 No Hit ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGTAAGGAG 32155 0.1872056590544516 No Hit GCTGTTACCGATTGAATGGTTCAATGAGTAGTAAGGAGTTTGGGGAATTT 32107 0.18692620417544015 No Hit GCTACCGCGAAAGGGTCCTAAGTAAAGCTTCACGGAGTGGCCGCACGGGG 31777 0.1850049518822363 No Hit GCACCTACCGATTGAATGACTCGGTGAGAAATCGGGACCGTGAGTCCGTT 30382 0.1768832944609656 No Hit GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTGTCATGT 30176 0.17568396727187474 No Hit GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCGTGTTG 29675 0.17276715697219255 No Hit GCTACTACCGATTGAATGTTTTAGTGAGATCTTCGGCTTGGTTTTCCGAG 28359 0.16510543570596153 No Hit ACACCATGGGAGTGGGGGATACCAGAAGTAGCTAGGATAACCGCAAGGGG 26737 0.15566218958603242 No Hit GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACGAGCAGGCGTTTT 26638 0.15508581389807127 No Hit GCTATTACCGATTGGGTGTATAGGTGAACCTTCCTGATAGATGCAAGTCT 26588 0.15479471506576767 No Hit GCTTTTACCGATTGAGTGCTCAGGTGAACCTTCTCGATAGTGCAAACTAG 25495 0.14843129459161075 No Hit GCACCTACCGATTGGACGATCCGGCAAGATGTTTGGAGTCGTGTTTCTTG 25239 0.14694086857021627 No Hit GCTACTATCGATTGGATGATTTGGTGAGGTCTTTGGACTGGTCGGCGAAG 24429 0.14222506748689778 No Hit GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTTTCATAT 24069 0.1401291558943118 No Hit GCTGTTACCGATTGATTATTTTGATGAGTATTGGGGAGTTTTAATGGATT 23734 0.13817879371787758 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTATAACCTTCGGGAG 23221 0.13519211969844255 No Hit GCACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG 23159 0.13483115714638608 No Hit ACACCATGGGAGTTGAATTCACCCGAAGCCGGAATACTAAACTAGTTACC 22846 0.1330088784561655 No Hit GCACCTACCGATTGGACGATCCGGCAAGATGTTTGGAGTTGTGTCTGTTG 22781 0.1326304499741708 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGCCTAACCTTCGGGAG 22428 0.13057529221810732 No Hit GCTCCTACCGATTTCGAGTGATCCGGTGAACCTTTTGGACTGCGCGGGGT 21845 0.12718107983344723 No Hit ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAATAGCTAAC 21076 0.1227039797926177 No Hit GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAA 20727 0.1206721099431385 No Hit GCTCCTACCGATTTCGAGTGATGTGGTGAACTATCTGGACTGTGGACGGG 20186 0.11752242057761343 No Hit ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGATAAAATAGCTACC 19232 0.11196825485726056 No Hit GCTACTACCGATTGAATGGCTTAGTGAGGGTTTTGGAGAGCTTTTGAAAA 19155 0.11151996265551299 No Hit GCTACCGGGGACTCTGAGCTGCGGCGAGAAGAATTTTTTTCTGATCGTCC 17897 0.10419591603475417 No Hit GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCGGTAGCAATA 17893 0.10417262812816988 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGGG 17461 0.10165753421706668 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.2705708919681583E-4 0.0 0.0 2 2.3287906584288802E-4 0.0 0.0 3 2.3870104248896023E-4 0.0 0.0 4 2.4452301913503244E-4 0.0 0.0 5 2.4452301913503244E-4 0.0 0.0 6 2.561669724271768E-4 0.0 0.0 7 2.561669724271768E-4 0.0 0.0 8 2.61988949073249E-4 0.0 0.0 9 2.6781092571932124E-4 0.0 0.0 10-11 2.7363290236539345E-4 0.0 0.0 12-13 2.969208089496822E-4 0.0 0.0 14-15 3.1729772721093494E-4 0.0 0.0 16-17 3.2603069218004326E-4 0.0 0.0 18-19 3.347636571491516E-4 0.0 0.0 20-21 3.6387354037951253E-4 0.0 0.0 22-23 3.7842848199469306E-4 0.0 0.0 24-25 3.842504586407653E-4 0.0 0.0 26-27 3.9007243528683743E-4 0.0 0.0 28-29 4.046273769020179E-4 0.0 5.821976646072201E-6 30-31 4.4538121342452334E-4 0.0 5.821976646072201E-6 32-33 4.7449109665488435E-4 0.0 5.821976646072201E-6 34-35 4.8031307330095657E-4 0.0 5.821976646072201E-6 36-37 5.094229565313175E-4 0.0 1.74659299382166E-5 38-39 5.414438280847147E-4 0.0 4.6575813168577606E-5 40-41 6.083965595145449E-4 0.0 6.40417431067942E-5 42-43 6.724383026213393E-4 0.0 9.315162633715521E-5 44-45 7.568569639893861E-4 0.0 1.3681645118269673E-4 46-47 8.15076730450108E-4 0.0 1.7174831105912992E-4 48-49 9.489821933097687E-4 0.0 2.3287906584288802E-4 50-51 0.0011149085277228263 0.0 2.9400982062664614E-4 52-53 0.0012924788154280286 0.0 3.2603069218004326E-4 54-55 0.0014642271264871584 0.0 3.842504586407653E-4 56-57 0.0016709072974227216 0.0 4.3955923677845113E-4 58-59 0.0018950533982965015 0.0 5.327108631156064E-4 60-61 0.0021686863006618947 0.0 5.880196412532922E-4 62-63 0.002465607109611577 0.0 6.607943493291948E-4 64-65 0.002753794953592151 0.0 6.899042325595558E-4 66-67 0.003018694890988436 0.0 7.510349873433139E-4 68-69 0.0033913013963370567 0.0 7.917888238658193E-4 70-71 0.0037842848199469304 0.0 8.500085903265413E-4 72-73 0.004188912196848948 0.0 9.02406380141191E-4 74-75 0.004683780211765085 0.0 9.606261466019131E-4 76-77 0.0052019361332655105 0.0 0.0010217569013856711 78-79 0.005670605253274324 0.0 0.0010654217262312127 80-81 0.0062382479762663635 0.0 0.001100353586107646 82-83 0.006960173080379316 0.0 0.001132374457661043 84-85 0.00757730260486297 0.0 0.0011643953292144401 86-87 0.008168233234439298 0.0 0.0012109711423830176 88-89 0.008965844034951188 0.0 0.0012342590489673065 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCATGGG 864355 0.0 94.98682 4 AGTAACG 323630 0.0 94.97652 7 CACCATG 863215 0.0 94.94222 2 ACCATGG 862260 0.0 94.92783 3 TTGTAGT 323260 0.0 94.922134 3 ACACCAT 855010 0.0 94.90223 1 TTCCGAT 68040 0.0 94.89529 6 CTATCCG 54245 0.0 94.89492 2 GCTATCC 54125 0.0 94.8947 1 TGTAGTA 323585 0.0 94.88403 4 CCGATTG 218625 0.0 94.882675 8 TATCCGG 54260 0.0 94.87744 3 CTGTCCG 22795 0.0 94.87498 2 CGATTGA 193720 0.0 94.83817 9 GCTGTTA 56520 0.0 94.82352 1 GCTATTA 38585 0.0 94.80304 1 TGGGAGT 835760 0.0 94.78005 7 CTACTAC 21410 0.0 94.75596 2 GCTGTCC 22810 0.0 94.75011 1 TGTTACC 56920 0.0 94.71627 3 >>END_MODULE