FastQCFastQC Report
Thu 16 Apr 2015
000000000-AEB23 l01n01.33100000008795.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AEB23 l01n01.33100000008795.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17176297
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG218187012.702796184765553No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC16284379.480722183599877No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGAATAA13046437.595601077461573No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCAAAG9324045.428434312704304No Hit
ACACCATGGGAGTCGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC6915844.026385896797197No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG6671083.8838871964079336No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAGTAGCTACC4168172.4266988396858764No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCATAA2450421.426628801306824No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACTGTATGTG2368591.3789875664120153No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGCTTAACCTTCGGGAG2309351.3444981767606836No Hit
GCTGTTACCGATTGATTATTTTGATGAGTATTGGGGAGTTTAATAATAGG2192291.2763461181417624No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTTTCAGCC2089921.2167465432159212No Hit
ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG1873671.0908462982446099No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTCAGCAATGA1791161.0428091689378682No Hit
GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGACGCAAGTTT1730221.0073300432567043No Hit
ACACCATGGGAATTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG1595810.9290768551568478No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGGACAGGAC1113570.648317853376662No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTTCGGGG1110150.6463267373637054No Hit
GCTGTCCGGGACTGAGCTGTTTCGAGAAGAGTGAGGACTGCTGCAGCGAG1014290.5905172692344572No Hit
ACACCATGGGAGTCTATTGCACCAGAAGTAGGTAGCCTAACGAAAGAGGG848490.49398889644258015No Hit
GTTGTTTCCGATGATGGTGGAATGCAGGTGATCGGACTGGCCTCGGCCGG745370.43395267326828363No Hit
GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTTGCAAAGCT721280.4199275315278957No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTCGGGAG700480.4078178201040655No Hit
GCTGTTACCGATTGAATGGTTCAATGAGTAGTAAGGAGTTTGAGGCAGCA676240.3937053487139865No Hit
GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACTAGCAGACGTTTT656270.38207886135178026No Hit
ACACCATGGGAGTGGGGGATACCAGAAGTGGGTAGGCTAACCGCAAGGAG653320.380361378241189No Hit
ACACCATGGGAGTGGAGGATACCAGAAGTAGCTAGACTAACCGCAAGGGG644270.3750924893764937No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACGGCATGTG644220.3750633794932633No Hit
GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGTGTCACCGCT564430.3286098278342532No Hit
ACACCATGGGAGTGGGTTGTACCAGAAGTAGTTAGTCTAACCCTTCGGGG557080.32433067499939017No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTAGGGGG549370.3198419310052685No Hit
GCTGCCCGGAACTGAGCCATTTCGAGAAAAATCGGGACTGACTGCTTGTG494320.287791949568641No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCCTTACCGC448460.2610923646697539No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGGGCGTAAGCTC401580.2337989381529674No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCCTAACCTTCGGGAG391870.2281457988296313No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACCGTATGTG380100.22129333231720436No Hit
GCTGTCCGGGACTGAGCTGTTTCGAGAAGACTGAGGACTGCTGCAGCGAG379710.22106627522800754No Hit
TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTGCTGA378130.2201464029179281No Hit
GCACCTACCGATTGAATGACTCGGTGAAAAATTGGGACCGTGAAACTGTT374700.21814946492832535No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAG369860.21533162823162644No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTCCGTGCC345340.2010561414954574No Hit
GCTATCCGGGACTGAGTTGTTTCGAGAGGAGTGGAGACTGTTGTTTTGGG328450.19122282294024143No Hit
GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACCGATGTGCGGAG326830.1902796627235777No Hit
ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGTAAGGAG321550.1872056590544516No Hit
GCTGTTACCGATTGAATGGTTCAATGAGTAGTAAGGAGTTTGGGGAATTT321070.18692620417544015No Hit
GCTACCGCGAAAGGGTCCTAAGTAAAGCTTCACGGAGTGGCCGCACGGGG317770.1850049518822363No Hit
GCACCTACCGATTGAATGACTCGGTGAGAAATCGGGACCGTGAGTCCGTT303820.1768832944609656No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTGTCATGT301760.17568396727187474No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCGTGTTG296750.17276715697219255No Hit
GCTACTACCGATTGAATGTTTTAGTGAGATCTTCGGCTTGGTTTTCCGAG283590.16510543570596153No Hit
ACACCATGGGAGTGGGGGATACCAGAAGTAGCTAGGATAACCGCAAGGGG267370.15566218958603242No Hit
GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACGAGCAGGCGTTTT266380.15508581389807127No Hit
GCTATTACCGATTGGGTGTATAGGTGAACCTTCCTGATAGATGCAAGTCT265880.15479471506576767No Hit
GCTTTTACCGATTGAGTGCTCAGGTGAACCTTCTCGATAGTGCAAACTAG254950.14843129459161075No Hit
GCACCTACCGATTGGACGATCCGGCAAGATGTTTGGAGTCGTGTTTCTTG252390.14694086857021627No Hit
GCTACTATCGATTGGATGATTTGGTGAGGTCTTTGGACTGGTCGGCGAAG244290.14222506748689778No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTTTCATAT240690.1401291558943118No Hit
GCTGTTACCGATTGATTATTTTGATGAGTATTGGGGAGTTTTAATGGATT237340.13817879371787758No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTATAACCTTCGGGAG232210.13519211969844255No Hit
GCACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG231590.13483115714638608No Hit
ACACCATGGGAGTTGAATTCACCCGAAGCCGGAATACTAAACTAGTTACC228460.1330088784561655No Hit
GCACCTACCGATTGGACGATCCGGCAAGATGTTTGGAGTTGTGTCTGTTG227810.1326304499741708No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGCCTAACCTTCGGGAG224280.13057529221810732No Hit
GCTCCTACCGATTTCGAGTGATCCGGTGAACCTTTTGGACTGCGCGGGGT218450.12718107983344723No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAATAGCTAAC210760.1227039797926177No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAA207270.1206721099431385No Hit
GCTCCTACCGATTTCGAGTGATGTGGTGAACTATCTGGACTGTGGACGGG201860.11752242057761343No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGATAAAATAGCTACC192320.11196825485726056No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGGTTTTGGAGAGCTTTTGAAAA191550.11151996265551299No Hit
GCTACCGGGGACTCTGAGCTGCGGCGAGAAGAATTTTTTTCTGATCGTCC178970.10419591603475417No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCGGTAGCAATA178930.10417262812816988No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGGG174610.10165753421706668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGGG8643550.094.986824
AGTAACG3236300.094.976527
CACCATG8632150.094.942222
ACCATGG8622600.094.927833
TTGTAGT3232600.094.9221343
ACACCAT8550100.094.902231
TTCCGAT680400.094.895296
CTATCCG542450.094.894922
GCTATCC541250.094.89471
TGTAGTA3235850.094.884034
CCGATTG2186250.094.8826758
TATCCGG542600.094.877443
CTGTCCG227950.094.874982
CGATTGA1937200.094.838179
GCTGTTA565200.094.823521
GCTATTA385850.094.803041
TGGGAGT8357600.094.780057
CTACTAC214100.094.755962
GCTGTCC228100.094.750111
TGTTACC569200.094.716273
Produced by FastQC (version 0.11.2)