000000000-AE77A l01n02 clinical flu pool f4.3420000000b78a.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AE77A l01n02 clinical flu pool f4.3420000000b78a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	588453
Sequences flagged as poor quality	0
Sequence length	300
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT	46007	7.818296448484416	Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATTT	1784	0.3031677975981089	Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATAT	1664	0.28277534484487293	Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGAACT	1124	0.19100930745531078	Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCC	682	0.11589710648089142	Illumina Single End PCR Primer 1 (96% over 32bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAGCGT	20290	0.0	98.3864	8
AAGAGCG	20575	0.0	97.30935	7
TCGGAAG	20930	0.0	96.431435	3
CGGAAGA	20860	0.0	96.40268	4
ATCGGAA	21170	0.0	95.61597	2
GAAGAGC	21270	0.0	94.06065	6
GAGCGTC	22015	0.0	90.87759	9
GGAAGAG	22415	0.0	89.51818	5
GATCGGA	22655	0.0	89.153824	1
AGACGTC	180	1.837725E-8	57.166668	8
GCCGTAT	4090	0.0	49.31149	55-59
GAAGACG	210	6.1945684E-8	49.000004	6
CGTATCA	3985	0.0	47.733498	60-64
CATTAAA	6150	0.0	44.79317	65-69
AAGACGT	230	1.267199E-7	44.739132	7
ATCATTA	5510	0.0	44.019966	60-64
CGCCGTA	4665	0.0	43.485535	55-59
TCGCCGT	4640	0.0	43.339653	55-59
CTCGGTG	6545	0.0	43.033157	45-49
TCTCGGT	6695	0.0	41.585957	45-49