##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AE77A l01n02 clinical flu pool f3.3420000000b710.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 687459 Sequences flagged as poor quality 0 Sequence length 300 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.9772335513827 34.0 34.0 34.0 33.0 34.0 2 33.26458305149834 34.0 34.0 34.0 34.0 34.0 3 33.30660301196144 34.0 34.0 34.0 34.0 34.0 4 33.279385388801366 34.0 34.0 34.0 34.0 34.0 5 33.34257752098671 34.0 34.0 34.0 34.0 34.0 6 36.73030391630628 38.0 38.0 38.0 35.0 38.0 7 36.786163247553674 38.0 38.0 38.0 35.0 38.0 8 36.8348076030716 38.0 38.0 38.0 36.0 38.0 9 36.75865178868849 38.0 38.0 38.0 36.0 38.0 10-14 36.85319822709427 38.0 38.0 38.0 36.0 38.0 15-19 36.791802856606715 38.0 38.0 38.0 35.4 38.0 20-24 36.749278138769 38.0 38.0 38.0 35.2 38.0 25-29 36.68684794293186 38.0 38.0 38.0 35.0 38.0 30-34 36.7358382099878 38.0 38.0 38.0 35.2 38.0 35-39 36.66127899991127 38.0 38.0 38.0 34.6 38.0 40-44 36.49217626069336 38.0 37.6 38.0 34.0 38.0 45-49 36.34710840937423 38.0 37.0 38.0 34.0 38.0 50-54 36.259131962778866 38.0 37.0 38.0 34.0 38.0 55-59 36.21729063115036 38.0 37.0 38.0 34.0 38.0 60-64 36.07706728692183 38.0 37.0 38.0 33.0 38.0 65-69 35.82540922440465 38.0 37.0 38.0 32.0 38.0 70-74 36.14594004878836 38.0 37.0 38.0 33.8 38.0 75-79 36.129253671855345 38.0 37.0 38.0 33.4 38.0 80-84 35.57519386610693 38.0 37.0 38.0 30.8 38.0 85-89 35.53784443872289 38.0 37.0 38.0 30.8 38.0 90-94 35.5202713179986 38.0 37.0 38.0 30.0 38.0 95-99 35.353043599691034 38.0 37.0 38.0 28.2 38.0 100-104 35.3550803757024 38.0 37.0 38.0 28.0 38.0 105-109 35.14383374135767 38.0 37.0 38.0 27.0 38.0 110-114 35.02295002320138 38.0 37.0 38.0 26.8 38.0 115-119 35.05502466328901 38.0 37.0 38.0 27.0 38.0 120-124 34.73974011541052 38.0 36.0 38.0 25.0 38.0 125-129 34.60294621206501 38.0 36.0 38.0 24.2 38.0 130-134 34.6294042262884 38.0 36.0 38.0 24.2 38.0 135-139 34.36565322441047 38.0 35.6 38.0 23.8 38.0 140-144 34.22753880595061 38.0 35.0 38.0 23.6 38.0 145-149 33.949192315469 38.0 34.6 38.0 22.8 38.0 150-154 33.79802053649745 37.8 34.0 38.0 22.6 38.0 155-159 33.520992815571546 37.0 34.0 38.0 21.8 38.0 160-164 33.21484539441625 37.0 33.0 38.0 20.2 38.0 165-169 32.45932862905279 37.0 30.4 38.0 17.2 38.0 170-174 31.930198019081864 36.6 28.8 38.0 16.6 38.0 175-179 32.208415047297365 37.0 29.8 38.0 16.4 38.0 180-184 32.1690340805779 37.0 29.8 38.0 16.2 38.0 185-189 31.90941627064305 37.0 28.6 38.0 15.4 38.0 190-194 31.156352306101166 35.8 27.8 37.8 10.6 38.0 195-199 30.815823780036332 35.0 26.6 37.4 10.0 38.0 200-204 30.111244743322874 35.0 24.4 37.0 10.0 37.8 205-209 29.447452720816806 34.4 23.4 37.0 10.0 37.4 210-214 28.82572458866638 33.8 22.2 37.0 10.0 37.2 215-219 28.038247808232928 32.2 20.8 37.0 9.8 37.0 220-224 26.966087286660002 30.6 19.8 36.4 9.2 37.0 225-229 26.29656604975715 29.4 19.0 36.2 9.0 37.0 230-234 25.348482745880116 28.0 17.0 35.4 9.0 37.0 235-239 24.324215989608106 26.0 15.0 34.0 8.0 37.0 240-244 23.69871483244819 25.0 14.6 33.4 8.0 36.8 245-249 22.576629006238917 23.2 12.2 32.2 8.0 36.2 250-254 21.311340749048306 21.4 10.6 30.8 8.0 36.0 255-259 20.333523308299114 19.8 9.0 29.4 8.0 35.6 260-264 19.90842072036296 19.4 9.0 28.6 8.0 34.8 265-269 19.159700578507227 18.4 9.0 27.4 7.8 34.0 270-274 18.19288859408343 16.8 8.2 26.0 7.0 33.2 275-279 17.1292784006028 14.8 8.0 24.0 7.0 31.4 280-284 16.20420068687732 13.4 8.0 22.2 7.0 30.2 285-289 15.840935386692152 13.2 8.0 21.6 7.0 29.6 290-294 15.404436046367858 13.0 8.0 21.0 7.0 29.0 295-299 14.763045650722443 12.6 8.0 20.2 7.0 27.2 300 12.532596125732589 11.0 8.0 15.0 7.0 22.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04760876917495693 1101 2 0.07644541730115151 1101 3 0.12286359031002547 1101 4 0.10612994482050908 1101 5 0.09863837079534221 1101 6 0.23124391622738472 1101 7 0.09461915976839208 1101 8 0.1440039972880527 1101 9 0.09406734418827511 1101 10-14 0.1563484443456602 1101 15-19 0.1803574143352904 1101 20-24 0.14635930084881466 1101 25-29 0.0907830541298793 1101 30-34 0.12990277079921242 1101 35-39 0.13874452277592297 1101 40-44 0.1612728222801323 1101 45-49 0.15078490895945862 1101 50-54 0.20356749228458426 1101 55-59 0.2408358090311964 1101 60-64 0.2886606909088343 1101 65-69 0.38157516562166194 1101 70-74 0.20156593877499773 1101 75-79 0.19005954566315353 1101 80-84 0.32468140997320916 1101 85-89 0.1385153033842741 1101 90-94 0.1755606497415343 1101 95-99 0.1490276863696991 1101 100-104 0.17597161760134838 1101 105-109 0.2763624583056057 1101 110-114 0.333077706840875 1101 115-119 0.28347240252907824 1101 120-124 0.16698185479573624 1101 125-129 0.32203553061177814 1101 130-134 0.22129029964960978 1101 135-139 0.2346596308067106 1101 140-144 0.18459185739150286 1101 145-149 0.43822061363410114 1101 150-154 0.27800213906005666 1101 155-159 0.29670299544395107 1101 160-164 0.3279283616151858 1101 165-169 0.604962484215875 1101 170-174 1.18813267957335 1101 175-179 0.26760704865789364 1101 180-184 -0.12145918244230813 1101 185-189 0.08443618535958564 1101 190-194 -0.538388456806242 1101 195-199 -0.5222735961619662 1101 200-204 -0.2750500114244936 1101 205-209 -0.17990095403593998 1101 210-214 -0.6371805687810728 1101 215-219 -0.5486860843793018 1101 220-224 -0.7894114819302089 1101 225-229 -0.48802593791427284 1101 230-234 -0.5216681197392994 1101 235-239 -0.5494819097461203 1101 240-244 -0.48279379758021435 1101 245-249 -0.28973467479695003 1101 250-254 -0.3006060574912297 1101 255-259 -0.17681449473234423 1101 260-264 -0.016308931924974956 1101 265-269 -0.19638996463034886 1101 270-274 -0.38213158048282025 1101 275-279 -0.12598053254712838 1101 280-284 0.26752457687697273 1101 285-289 -0.15295511753639346 1101 290-294 -0.08597225046750623 1101 295-299 -0.011453900990506583 1101 300 0.7584294610893192 1102 1 -0.05516164605277396 1102 2 -0.03636345063168278 1102 3 0.002884097615677206 1102 4 0.03824726686297453 1102 5 0.07997895301715374 1102 6 0.2162215010551094 1102 7 0.2041129210746817 1102 8 0.045340769804646186 1102 9 -0.0012994635138383615 1102 10-14 0.06011667682930977 1102 15-19 0.008843641226150112 1102 20-24 -0.13246030538920195 1102 25-29 -0.02751787762034752 1102 30-34 0.08982171811497608 1102 35-39 0.03262307395466735 1102 40-44 0.05288176568370773 1102 45-49 -0.04212958184510285 1102 50-54 -0.10076767497172057 1102 55-59 0.04682699245704214 1102 60-64 0.08440358644153889 1102 65-69 0.1548749791751689 1102 70-74 0.034778129108346434 1102 75-79 0.10072623804934011 1102 80-84 0.044080043181182305 1102 85-89 0.0407366870426884 1102 90-94 0.03185085283136857 1102 95-99 -0.08078566821439637 1102 100-104 -0.07148242029436602 1102 105-109 0.031242467661073192 1102 110-114 0.11075856084174518 1102 115-119 0.06535056309248688 1102 120-124 -0.15697522332914104 1102 125-129 0.06050969544542539 1102 130-134 -0.067972721314014 1102 135-139 -0.08792046794422959 1102 140-144 -0.10867937131679639 1102 145-149 -0.012316874287265023 1102 150-154 -0.2235286149662059 1102 155-159 0.08806864817847071 1102 160-164 -0.00491678290948272 1102 165-169 0.19622789106767868 1102 170-174 1.0766776434384084 1102 175-179 0.48438247075453944 1102 180-184 0.23804214402230173 1102 185-189 0.10233987094799346 1102 190-194 -0.04877333951012375 1102 195-199 -0.25891223055143087 1102 200-204 0.0745231750021027 1102 205-209 0.024374596156025774 1102 210-214 0.301210710851894 1102 215-219 0.24913267853948184 1102 220-224 -0.14818414199057628 1102 225-229 0.23179152174776618 1102 230-234 -0.2482103119232555 1102 235-239 0.14378817363940755 1102 240-244 0.13006504259360696 1102 245-249 0.3764743851884198 1102 250-254 0.9506676318526281 1102 255-259 0.9129890583000169 1102 260-264 0.710689400123389 1102 265-269 0.3641012823910579 1102 270-274 0.5263865466612678 1102 275-279 0.639316006588853 1102 280-284 0.7740664194360818 1102 285-289 0.5387330339789571 1102 290-294 0.8331199787585994 1102 295-299 0.5055139353320186 1102 300 0.6853979544654347 1103 1 0.039335768528310666 1103 2 0.14707279548974128 1103 3 0.10253551767990388 1103 4 0.004528763270919001 1103 5 0.11159551689126346 1103 6 0.2957793031950473 1103 7 0.09111464969692662 1103 8 0.17357832957075914 1103 9 0.08899467278671835 1103 10-14 0.10922577961395064 1103 15-19 0.005990197192048186 1103 20-24 -0.05317488674310766 1103 25-29 -0.016106686452033614 1103 30-34 0.04158044320872989 1103 35-39 0.09254714202108971 1103 40-44 0.03344545103057328 1103 45-49 -0.10234767499834163 1103 50-54 -0.15642492263600616 1103 55-59 -0.0030036169912577293 1103 60-64 -0.07999785669202453 1103 65-69 0.11797732557471363 1103 70-74 0.07707217403329025 1103 75-79 0.13315033391919684 1103 80-84 0.4795004777143106 1103 85-89 0.34643560980234156 1103 90-94 0.1847968597620948 1103 95-99 0.19699505856440425 1103 100-104 0.157919547347241 1103 105-109 0.21583178987944507 1103 110-114 0.22439207308894993 1103 115-119 0.24824192199515238 1103 120-124 0.30377110503935967 1103 125-129 0.36044576257284433 1103 130-134 0.42247693387829344 1103 135-139 0.33687701032927464 1103 140-144 0.3116972104356748 1103 145-149 0.6346241015742393 1103 150-154 0.6042723557193526 1103 155-159 0.4965599676453536 1103 160-164 0.6938060324693893 1103 165-169 0.9843273594359374 1103 170-174 1.8444448953197963 1103 175-179 1.0643541383024058 1103 180-184 0.9709936635476808 1103 185-189 0.8820540636549552 1103 190-194 0.8317217840678879 1103 195-199 0.8806126222631931 1103 200-204 0.9212924608926585 1103 205-209 1.0240286913616004 1103 210-214 0.8677842485339688 1103 215-219 0.8074894444078069 1103 220-224 0.930343941132378 1103 225-229 0.9505600429686929 1103 230-234 1.0356615614301425 1103 235-239 1.0192686996623692 1103 240-244 0.9527166968078404 1103 245-249 1.1213030514020197 1103 250-254 1.259547989011054 1103 255-259 1.5497547582988425 1103 260-264 1.0653355946446403 1103 265-269 1.0361688690859268 1103 270-274 1.1957501365446639 1103 275-279 1.4579797163726873 1103 280-284 1.5316830428245716 1103 285-289 1.2226145859336626 1103 290-294 1.0132963397067094 1103 295-299 1.0610108849194297 1103 300 1.0521057301395018 2116 1 0.0037658994423566128 2116 2 -0.11710836072884234 2116 3 0.019008793816396974 2116 4 -0.029092112954543836 2116 5 0.03652002414258959 2116 6 0.06102907823054693 2116 7 0.02081751671278198 2116 8 -0.06297760640443784 2116 9 -0.03193131164652385 2116 10-14 -0.00848949237277452 2116 15-19 -0.1155300172391236 2116 20-24 -0.1443993830234831 2116 25-29 -0.13614774059878698 2116 30-34 -0.06545697373726256 2116 35-39 -0.18837407034719433 2116 40-44 -0.04673950845183583 2116 45-49 -0.09433660093002061 2116 50-54 -0.03887109752020024 2116 55-59 -0.06036287854418276 2116 60-64 -0.24369329343488033 2116 65-69 -0.08492422180931669 2116 70-74 -0.13641514432398338 2116 75-79 -0.031411028781121786 2116 80-84 0.10229997724699302 2116 85-89 0.016362907752444755 2116 90-94 -0.03125010790837024 2116 95-99 0.03665523478309041 2116 100-104 0.027520834126171678 2116 105-109 0.17130500666488757 2116 110-114 0.07426470811409303 2116 115-119 -0.008203992703585072 2116 120-124 0.16930408654280882 2116 125-129 0.19960780954890822 2116 130-134 0.18571500776256755 2116 135-139 0.1581678538996698 2116 140-144 0.3278718128585254 2116 145-149 0.23634804001459742 2116 150-154 0.44864944237369997 2116 155-159 0.278398663456791 2116 160-164 0.2989175974728937 2116 165-169 0.8793567382101983 2116 170-174 1.5726518158930958 2116 175-179 1.0739324400347954 2116 180-184 0.6254645668408259 2116 185-189 0.6366696276608792 2116 190-194 0.8757183045371058 2116 195-199 0.5364598106716372 2116 200-204 0.7177477638498431 2116 205-209 0.896259230059389 2116 210-214 0.6770057844554422 2116 215-219 1.263385312674835 2116 220-224 1.4043219188919203 2116 225-229 0.6277585051168089 2116 230-234 1.0909243517332605 2116 235-239 0.8722821447755322 2116 240-244 1.0274061079194396 2116 245-249 0.8487460818790886 2116 250-254 1.261417187669835 2116 255-259 1.5191906414695815 2116 260-264 1.371971582608964 2116 265-269 0.9596077413918245 2116 270-274 1.1504034694063883 2116 275-279 1.2538081762246946 2116 280-284 1.3468114479417146 2116 285-289 1.0367624995640838 2116 290-294 1.0761305865867286 2116 295-299 0.7908587124008903 2116 300 0.4648087152579734 1117 1 0.06006179444216997 1117 2 -0.023817310726954588 1117 3 0.01331391039916241 1117 4 0.016048813083529012 1117 5 -0.026713349565731903 1117 6 0.07283107206075101 1117 7 0.1757072267699371 1117 8 0.1159348240433502 1117 9 0.10391197348917558 1117 10-14 0.12332603338519732 1117 15-19 0.12303077234412996 1117 20-24 0.2135561839926865 1117 25-29 0.2552816047587214 1117 30-34 0.292990545093879 1117 35-39 0.20128524207947152 1117 40-44 0.23545843620920692 1117 45-49 0.27539990499374767 1117 50-54 0.20902187250137416 1117 55-59 0.27810955133583093 1117 60-64 0.1862453870602181 1117 65-69 0.3952142438782644 1117 70-74 0.3156346351907615 1117 75-79 0.35751396364856447 1117 80-84 0.31877746503987225 1117 85-89 0.32160392747310595 1117 90-94 0.3424081730636601 1117 95-99 0.23726559096615318 1117 100-104 0.38118711123733817 1117 105-109 0.42669116340814384 1117 110-114 0.5266012130915811 1117 115-119 0.3427169827307637 1117 120-124 0.5092052940071312 1117 125-129 0.49615988854507975 1117 130-134 0.5024521335054146 1117 135-139 0.4467071154159967 1117 140-144 0.6073178318221437 1117 145-149 0.7253373800729008 1117 150-154 0.6619485831436052 1117 155-159 0.7925158707697051 1117 160-164 1.0463184973934005 1117 165-169 1.3900392725604718 1117 170-174 1.864019534201887 1117 175-179 1.2821886902713686 1117 180-184 0.9277256052980007 1117 185-189 1.0564351136747305 1117 190-194 1.2430938202644235 1117 195-199 1.207957004925774 1117 200-204 1.1497443855993374 1117 205-209 0.9718076043354813 1117 210-214 0.7487803595718425 1117 215-219 1.0088270402194084 1117 220-224 1.5908865125555671 1117 225-229 1.5428499166009644 1117 230-234 1.1738188831311192 1117 235-239 1.4179179797806398 1117 240-244 1.151213902499098 1117 245-249 1.260836693749333 1117 250-254 1.0342281854365964 1117 255-259 1.1244192411516316 1117 260-264 1.5365718702807172 1117 265-269 1.2378187353734305 1117 270-274 1.006881643258538 1117 275-279 1.2592496908543183 1117 280-284 1.319272910336199 1117 285-289 0.8930625345767638 1117 290-294 0.7917027127080836 1117 295-299 0.6367670785388988 1117 300 0.8857610838184797 2117 1 0.031093603755138588 2117 2 -2.874713213003588E-4 2117 3 -0.03781632392600187 2117 4 -0.04668628597466551 2117 5 0.04506866611848892 2117 6 0.011654310400771806 2117 7 -0.14020141643294437 2117 8 0.07292752600574914 2117 9 -0.08998670761096861 2117 10-14 -0.0191661573393489 2117 15-19 -0.023929473050046113 2117 20-24 -0.12497532045367876 2117 25-29 -0.14066214500676466 2117 30-34 -0.03558342961625982 2117 35-39 0.023418206133349884 2117 40-44 -0.017837406348292006 2117 45-49 0.026211553712364832 2117 50-54 0.19353992529489972 2117 55-59 0.14516226511767627 2117 60-64 0.16942613074713364 2117 65-69 0.23047921986415076 2117 70-74 0.19525723377991966 2117 75-79 0.3593512539941415 2117 80-84 0.22704099173680703 2117 85-89 0.0971034396865349 2117 90-94 0.18384552532060638 2117 95-99 0.2912626267246523 2117 100-104 0.26804560153789936 2117 105-109 0.2917291779291631 2117 110-114 0.22169133635149763 2117 115-119 0.2861400719206699 2117 120-124 0.5222085584249783 2117 125-129 0.44716606561161143 2117 130-134 0.47170082492412035 2117 135-139 0.5314518248077604 2117 140-144 0.6114861791636983 2117 145-149 0.8518416622761862 2117 150-154 0.7249237060282994 2117 155-159 0.8454796432360325 2117 160-164 0.9904815916487735 2117 165-169 1.2258335438823735 2117 170-174 1.542223213238323 2117 175-179 1.1175664669026375 2117 180-184 0.7093976110388667 2117 185-189 0.5279029994951543 2117 190-194 0.9173745695796462 2117 195-199 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20-24 0.15216568309839573 2109 25-29 0.13185375074792205 2109 30-34 0.11824632908142263 2109 35-39 0.15855314936258225 2109 40-44 0.0467511550199049 2109 45-49 0.16618146450679916 2109 50-54 0.08850019577002399 2109 55-59 0.10529868003556686 2109 60-64 0.15099816125872678 2109 65-69 0.16724970986668097 2109 70-74 -0.03204401694302561 2109 75-79 0.13259443704126994 2109 80-84 0.028459153406515725 2109 85-89 -0.22491678526031933 2109 90-94 0.07741457968327126 2109 95-99 0.15079762166022448 2109 100-104 -0.03244330253652805 2109 105-109 0.05256374980735501 2109 110-114 -0.1312265822619949 2109 115-119 -0.12742203971284027 2109 120-124 -0.013187344029674364 2109 125-129 -0.061440369642888015 2109 130-134 0.10913190589612043 2109 135-139 0.0169596059442938 2109 140-144 -0.06612612888713443 2109 145-149 -0.18581596771640818 2109 150-154 0.032176673225720265 2109 155-159 -0.01550646705747738 2109 160-164 -0.010457193992635894 2109 165-169 -3.664637499657154 2109 170-174 -4.536323875033254 2109 175-179 -0.08920827734215209 2109 180-184 -0.395868364065624 2109 185-189 -0.915147201170349 2109 190-194 -0.8880561739660102 2109 195-199 -0.5713526788059831 2109 200-204 -0.8211516516720394 2109 205-209 -0.4409428908853954 2109 210-214 -0.2379936370048874 2109 215-219 -0.9757908223495875 2109 220-224 -1.3918634537886447 2109 225-229 -0.8161554106294489 2109 230-234 -0.4514317923886253 2109 235-239 -0.9574036132920369 2109 240-244 -0.9444391520040476 2109 245-249 -1.5691694243921006 2109 250-254 -1.1224675133537225 2109 255-259 -0.7480910118437372 2109 260-264 -0.5513682993444533 2109 265-269 -0.4807293298995674 2109 270-274 -0.21567837222312392 2109 275-279 -0.25381434409000647 2109 280-284 -0.06736075362518079 2109 285-289 -0.09216852442664347 2109 290-294 -0.5551865309021267 2109 295-299 0.5114752948783003 2109 300 1.202623414818346 2108 1 -0.27565474322702244 2108 2 -0.07890416742423412 2108 3 -0.1353469586010121 2108 4 0.07610333617792975 2108 5 0.04053019574703853 2108 6 0.15131647079773103 2108 7 0.13644903446743228 2108 8 0.015553848283325067 2108 9 0.06448411099663787 2108 10-14 0.09078238843632391 2108 15-19 0.08056100011131662 2108 20-24 0.1317471748665895 2108 25-29 0.20870924343100228 2108 30-34 0.09474211905564545 2108 35-39 0.13657357310241025 2108 40-44 0.25014128532066593 2108 45-49 0.2885852040946162 2108 50-54 0.18191633764318027 2108 55-59 0.20582180754022517 2108 60-64 0.18988715913899767 2108 65-69 -4.548891883020598 2108 70-74 -0.05614674537106623 2108 75-79 0.30039196717193306 2108 80-84 -0.019101946385063684 2108 85-89 0.02923860469061168 2108 90-94 0.1215235175061764 2108 95-99 0.06985526346657878 2108 100-104 -0.1318042704796767 2108 105-109 -0.12262046898755585 2108 110-114 -0.23497155736991715 2108 115-119 -0.33230142828985265 2108 120-124 -0.027115197338481778 2108 125-129 -0.0325730361357941 2108 130-134 -0.1276818337446315 2108 135-139 -0.23989098524054953 2108 140-144 -0.7495841950747888 2108 145-149 -0.5258796124568192 2108 150-154 -0.3759372059551538 2108 155-159 -0.44521035615033355 2108 160-164 -0.5054910171332594 2108 165-169 -4.093820098528688 2108 170-174 -4.173315488613341 2108 175-179 -0.29649537902145795 2108 180-184 -0.39156980614620096 2108 185-189 -0.8965064981198658 2108 190-194 -0.1921589522186835 2108 195-199 -0.22310701969927393 2108 200-204 -0.058032405443999124 2108 205-209 -0.5102003761801264 2108 210-214 -0.3594218102812583 2108 215-219 -0.5367942655431222 2108 220-224 -0.8327980019710886 2108 225-229 -0.7773372260086369 2108 230-234 -0.729202750419784 2108 235-239 -0.8802895930451733 2108 240-244 -0.9649479051021288 2108 245-249 -0.17709957523928566 2108 250-254 -0.5528084103777253 2108 255-259 0.10278454708743823 2108 260-264 -0.4594345025797537 2108 265-269 -0.6359804569483352 2108 270-274 -0.49888756436963533 2108 275-279 -0.5899774674467686 2108 280-284 -0.0791638977463549 2108 285-289 -0.5676267475619028 2108 290-294 -0.45555657222231716 2108 295-299 0.6786738706411715 2108 300 1.5557221800082406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 54.0 11 170.0 12 454.0 13 827.0 14 984.0 15 1157.0 16 2587.0 17 8109.0 18 14193.0 19 7921.0 20 5641.0 21 6019.0 22 7209.0 23 9345.0 24 12061.0 25 15829.0 26 20474.0 27 26609.0 28 34792.0 29 46543.0 30 61096.0 31 79923.0 32 97175.0 33 101893.0 34 80959.0 35 38730.0 36 6653.0 37 50.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.455291151908696 28.79939021818028 12.775889180300206 23.969429449610814 2 28.67269175325365 27.26882621363601 21.912579513832824 22.145902519277513 3 38.03615924731511 22.523670502531786 25.304199959561224 14.135970290591876 4 38.629940112792184 21.02816313409236 21.330435705983923 19.01146104713154 5 40.96404294656117 22.21805227657213 21.570450019564802 15.24745475730189 6 30.431342087309933 23.07715805597134 31.40580020044832 15.08569965627041 7 16.1280890933132 37.7837805600043 31.46980401740322 14.618326329279274 8 26.540782795774003 34.39492391546259 22.478867830663358 16.585425458100048 9 18.348003299105837 23.017518135626997 31.57046456588684 27.064013999380325 10-14 23.588752201949497 33.97398244840783 26.415044388101688 16.022220961540977 15-19 24.58159686614038 29.94622224743585 24.496180863149657 20.976000023274118 20-24 21.46458188779258 28.936503849684126 29.96053582831849 19.638378434204803 25-29 18.437783198707123 29.718281381144184 32.78883540691154 19.05510001323715 30-34 18.61134991323119 29.940374625977697 31.933366208021134 19.514909252769982 35-39 20.094114703567776 31.28014907070822 29.668125662766798 18.957610562957207 40-44 19.85450768700388 29.281804442155824 31.135005869440942 19.728682001399356 45-49 20.347133429048135 29.809137708576078 30.451270548498165 19.39245831387763 50-54 19.694032662311496 29.85204935857993 31.97211760992292 18.481800369185652 55-59 22.107558414392713 30.957860759696214 27.680458034588245 19.25412279132283 60-64 19.012042900012947 29.399629650640986 29.292830554258508 22.29549689508756 65-69 17.643292181788294 30.27686014729606 31.232466227076817 20.847381443838835 70-74 19.145170839279142 33.46343563761621 26.407451207999312 20.983942315105335 75-79 18.485698783491088 36.25164555268023 26.427583317696033 18.83507234613264 80-84 20.17039561632039 32.79264654328477 26.332072167212882 20.704885673181963 85-89 20.273703595414418 32.58064844594368 26.466625646038526 20.679022312603372 90-94 19.04634312737196 31.379151338479822 28.62288514660511 20.951620387543112 95-99 19.159048030500728 30.61555670956377 28.405155798382157 21.820239461553342 100-104 19.7482031655706 29.635250974967235 28.607640601112212 22.008905258349955 105-109 20.145695961504615 29.58960461642076 29.78688183586221 20.477817586212414 110-114 18.990165231671998 28.892923068866654 30.364952673541257 21.751959025920094 115-119 18.096642854337492 28.82222794377556 30.895544316097396 22.185584885789552 120-124 19.282429934003336 29.793165846981417 29.20584354848798 21.71856067052726 125-129 21.098072757793556 30.15792941833622 29.503984964921543 19.24001285894868 130-134 21.387340917785643 29.46648454671479 29.29579800395369 19.850376531545884 135-139 20.116457854213852 28.41056703017926 30.530024335996764 20.94295077961013 140-144 18.217726438958543 29.97339477699761 31.043044021534378 20.76583476250947 145-149 18.938147584074105 31.225193066059216 28.83686154374297 20.99979780612371 150-154 19.80897769903369 27.866505493418515 30.526824145149018 21.797692662398777 155-159 19.336193140245456 29.195864771571834 31.36931802478402 20.09862406339869 160-164 21.814071821010415 28.19251766287153 28.40620313356869 21.58720738254936 165-169 19.89494646226175 28.180240567073817 30.460434731380342 21.464378239284088 170-174 18.671926230342823 30.860758491999814 31.21120858442717 19.256106693230198 175-179 19.49457349456477 31.102858497743135 29.23778727167729 20.164780736014805 180-184 22.056355360828793 28.61241179473976 29.23723451143995 20.093998332991493 185-189 19.847973390688544 29.732001316151983 28.784831552038188 21.635193741121284 190-194 20.254386079751665 29.50791247187105 28.976040752975813 21.26166069540147 195-199 19.684472635879455 31.27196314076222 30.29849361110618 18.74507061225214 200-204 20.513601538419017 28.436110371673074 29.67894812636099 21.371339963546916 205-209 22.33843763773549 28.63853698911499 29.442337652136345 19.580687721013177 210-214 21.90650552440379 28.853045447959936 28.720993898107057 20.51945512952922 215-219 20.976087301206327 29.07227921956073 29.139890524380363 20.81174295485258 220-224 21.996133587603044 28.527373996121952 29.679529979242396 19.796962437032608 225-229 21.28438205042046 29.202439709131745 29.564003089638803 19.949175150808994 230-234 19.864643485440432 29.743667473212206 28.48162783911642 21.910061202230942 235-239 22.159931620629482 30.22511312677938 26.68055161996617 20.934403632624964 240-244 19.906670797822123 30.281776804143956 29.08365444339226 20.72789795464166 245-249 23.704249998909027 27.544420830915005 28.184866297481015 20.566462872694956 250-254 22.329855307734718 30.263855735396582 26.70931648287389 20.696972473994812 255-259 22.788565656589167 28.959786390108032 27.85272368322291 20.398924270079892 260-264 21.49559464637164 30.353199245336814 27.708008768522923 20.443197339768627 265-269 22.49899994036008 30.533166341556367 27.225856378344016 19.741977339739535 270-274 22.416289553267905 29.360180025281508 27.95055414213793 20.272976279312658 275-279 22.291459502736746 29.463903873221202 26.40431688415364 21.84031973988841 280-284 22.496759806766658 28.995009156909724 27.04804213778567 21.46018889853795 285-289 22.858148631409293 30.119992610468405 26.99116601862802 20.03069273949428 290-294 21.163763948104542 31.859150872997517 25.1462269022589 21.83085827663904 295-299 22.380767434857933 30.551349244100372 26.06520534315501 21.00267797788668 300 13.437184526181964 41.54188328596074 27.56299293920501 17.45793924865228 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.25 10 0.0 11 0.25 12 0.25 13 0.0 14 0.25 15 0.75 16 1.5 17 1.75 18 3.5 19 4.25 20 4.75 21 6.0 22 9.75 23 15.0 24 15.75 25 17.5 26 26.5 27 33.0 28 38.5 29 54.25 30 76.75 31 133.25 32 335.25 33 1097.5 34 3879.25 35 6825.0 36 9912.0 37 12876.75 38 14916.25 39 15533.5 40 19329.0 41 24556.0 42 25821.0 43 23579.75 44 22812.25 45 19220.75 46 13220.5 47 7362.5 48 4065.25 49 1960.25 50 791.75 51 292.25 52 126.25 53 62.75 54 35.0 55 28.0 56 17.75 57 12.5 58 11.25 59 11.25 60 7.25 61 9.25 62 7.75 63 3.75 64 3.5 65 4.5 66 4.25 67 3.75 68 3.5 69 2.25 70 3.0 71 2.0 72 3.0 73 1.75 74 1.75 75 2.75 76 4.25 77 1.75 78 2.0 79 2.5 80 2.25 81 2.0 82 2.0 83 1.5 84 0.5 85 0.0 86 0.5 87 0.75 88 0.75 89 1.25 90 0.5 91 0.25 92 1.0 93 0.75 94 0.25 95 0.5 96 0.5 97 0.25 98 0.75 99 0.5 100 0.6666666666666666 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 2.909264407041002E-5 175-179 0.0 180-184 0.0 185-189 5.818528814082004E-5 190-194 0.0 195-199 5.818528814082004E-5 200-204 0.0 205-209 0.0 210-214 2.909264407041002E-5 215-219 0.0 220-224 0.0 225-229 0.0 230-234 5.818528814082004E-5 235-239 2.909264407041002E-5 240-244 0.0 245-249 0.0 250-254 0.0 255-259 2.909264407041002E-5 260-264 0.0 265-269 0.0 270-274 0.0 275-279 2.909264407041002E-5 280-284 0.0 285-289 0.0 290-294 0.0 295-299 0.0 300 1.4546322035205008E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 300 687459.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.362324264911868 #Duplication Level Percentage of deduplicated Percentage of total 1 69.77017992581797 9.322917681900243 2 3.4845410173532922 0.9312313397652117 3 1.374258286990086 0.5508985456351153 4 1.0707045247526885 0.5722840420661512 5 1.2348102700772419 0.8249967617207754 6 0.9552855953555481 0.7658901534444132 7 1.122594239263973 1.0500327770077085 8 1.0942130539401178 1.1696983713317883 9 1.016941688159955 1.2229834136130184 >10 17.72112051908179 51.82520790731463 >50 1.0498766415243543 8.560542779501054 >100 0.06316087994030194 1.6275128288257157 >500 0.010849654561383665 1.081233206515442 >1k 0.0206141503601835 9.136629759634985 >5k 0.009764597539008091 9.108359587255016 >10k+ 0.0010849552821120103 2.249580844468717 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT 15517 2.2571527902027615 Illumina Single End PCR Primer 1 (96% over 32bp) CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT 9443 1.373609189784409 No Hit GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCT 7489 1.0893740572165032 No Hit GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC 7304 1.0624633614513739 No Hit GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT 7024 1.0217336597527997 No Hit GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA 7016 1.0205699539899833 No Hit GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA 6927 1.0076237273786508 No Hit GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC 6448 0.9379468448300189 No Hit CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT 5985 0.8705973738070197 No Hit GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT 5191 0.755099576847492 No Hit CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA 4986 0.7252796166753217 No Hit CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC 4972 0.723243131590393 No Hit CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA 4601 0.6692762768397824 No Hit CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG 4591 0.6678216446362619 No Hit CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG 4526 0.6583665353133786 No Hit GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC 4517 0.6570573663302102 No Hit CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG 4413 0.6419291914135971 No Hit CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT 3951 0.5747251836109498 No Hit GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC 3829 0.5569786707279998 No Hit GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT 3715 0.5403958636078661 No Hit CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT 3070 0.4465720864807937 No Hit CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG 2743 0.39900561342567337 No Hit CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT 2635 0.383295585627652 No Hit GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTACTTTCT 2311 0.3361655022335877 No Hit GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCT 2013 0.29281746256867686 No Hit GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA 1794 0.2609610173115779 No Hit GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA 1582 0.23012281459694325 No Hit GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT 1484 0.21586741900244236 No Hit GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT 1289 0.18750209103379256 No Hit GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTA 970 0.1410993237414886 No Hit GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT 964 0.1402265444193763 No Hit GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA 867 0.12611661204522742 No Hit CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT 815 0.11855252458692082 No Hit GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT 765 0.11127936356931832 No Hit GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA 704 0.10240610712784326 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.007127697797250455 0.0 0.0 2 0.007418624237954554 0.0 0.0 3 0.012073447289220158 0.0 0.0 4 0.01600095423872551 0.0 0.0 5 0.019346608306822662 0.0 0.0 6 0.024001431358088264 0.0 0.0 7 0.030692739494282566 0.0 0.0 8 0.04000238559681377 0.0 0.0 9 0.05483963407272288 0.0 0.0 10-14 0.11625420570535842 0.0 0.0 15-19 0.19454251089883176 0.0 0.0 20-24 0.255578878158552 0.0 0.0 25-29 0.302127108671208 0.0 0.0 30-34 0.3429440883019933 0.0 0.0 35-39 0.3738113836606983 0.0 0.0 40-44 0.40240945278191137 0.0 0.0 45-49 0.43286945112363073 0.0 0.0 50-54 0.4636785611941948 0.0 0.0 55-59 0.49224753767133744 0.0 0.0 60-64 0.5202637539111423 0.0 0.0 65-69 0.5534293681514098 0.0 0.0 70-74 0.5947700153754625 0.0 0.0 75-79 0.6423655810746531 0.0 0.0 80-84 0.6906593702315338 0.0 0.0 85-89 0.7490773995249171 0.0 0.0 90-94 0.8163104999716346 0.0 0.0 95-99 0.8969262166907409 0.0 0.0 100-104 0.9892662689702222 0.0 0.0 105-109 1.0925742480642482 0.0 0.0 110-114 1.2099048816002118 0.0 0.0 115-119 1.3488804423245604 0.0 0.0 120-124 1.511479230034082 0.0 0.0 125-129 1.6914172336095679 0.0 0.0 130-134 1.8896836029494122 0.0 0.0 135-139 2.1191081940886654 0.0 0.0 140-144 2.387807854722973 0.0 0.0 145-149 2.6915059661739824 0.0 0.0 150-154 3.0360210572557778 0.0 0.0 155-159 3.4417179788176453 0.0 0.0 160-164 3.8294356463440002 0.0 0.0 165-169 4.258203034653704 0.0 0.0 170-174 4.757956474495207 0.0 0.0 175-179 5.323430197291765 0.0 0.0 180-184 5.959657230467562 0.0 0.0 185-189 6.650403878631307 0.0 0.0 190-194 7.404514305580404 0.0 0.0 195-199 8.214657165009113 0.0 0.0 200-204 9.104746610343309 0.0 0.0 205-209 10.058461668259488 0.0 0.0 210-214 11.079671660419022 0.0 0.0 215-219 12.156419510108966 0.0 0.0 220-224 13.26339461698807 0.0 0.0 225-229 14.382384985868248 0.0 0.0 230-234 15.53055527675105 0.0 0.0 235-239 16.670085052344938 0.0 0.0 240-244 17.785758859801096 0.0 1.1637057628164007E-4 245-249 18.893432190137887 0.0 1.4546322035205008E-4 250-254 19.94870966850387 0.0 1.4546322035205008E-4 255-259 21.003812591005424 0.0 1.4546322035205008E-4 260-264 22.021473280588367 0.0 1.4546322035205008E-4 265-269 22.97163903592796 0.0 1.4546322035205008E-4 270-274 23.82678821573359 0.0 1.4546322035205008E-4 275-279 24.655666737943644 0.0 1.4546322035205008E-4 280-284 25.384233823398922 0.0 1.4546322035205008E-4 285-288 26.002241588225623 0.0 1.4546322035205008E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGGTT 6530 0.0 285.67075 1 CGGGTTA 6695 0.0 279.06946 2 GGGGGGA 8770 0.0 269.52795 1 GGGTTAT 7055 0.0 264.8292 3 GGAGCAG 9145 0.0 257.67197 5 CAGAAGC 9230 0.0 255.61755 9 GAGCAGA 9210 0.0 255.53421 6 GGGGAGC 9415 0.0 252.4684 3 ATTAGTA 7475 0.0 249.3592 8 TATTAGT 7485 0.0 249.22244 7 TTATTAG 7580 0.0 245.90501 6 GGTTATT 7680 0.0 243.27734 4 GGGAGCA 10020 0.0 236.93114 4 TTAGTAG 8350 0.0 223.05269 9 GTTATTA 8580 0.0 217.41608 5 GGGGGAG 11450 0.0 207.85414 2 GCAGAAG 11640 0.0 203.70361 8 AGCAGAA 14170 0.0 166.50317 7 GGGTAGC 80 6.2245796E-5 73.5 3 CGCAGAA 145 2.7054557E-7 60.82759 7 >>END_MODULE