FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n02 clinical flu pool f3.3420000000b710.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n02 clinical flu pool f3.3420000000b710.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687459
Sequences flagged as poor quality0
Sequence length300
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT155172.2571527902027615Illumina Single End PCR Primer 1 (96% over 32bp)
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT94431.373609189784409No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCT74891.0893740572165032No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC73041.0624633614513739No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT70241.0217336597527997No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA70161.0205699539899833No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA69271.0076237273786508No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC64480.9379468448300189No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT59850.8705973738070197No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT51910.755099576847492No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA49860.7252796166753217No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC49720.723243131590393No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA46010.6692762768397824No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG45910.6678216446362619No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG45260.6583665353133786No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC45170.6570573663302102No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG44130.6419291914135971No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT39510.5747251836109498No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC38290.5569786707279998No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT37150.5403958636078661No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT30700.4465720864807937No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG27430.39900561342567337No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT26350.383295585627652No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTACTTTCT23110.3361655022335877No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCT20130.29281746256867686No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA17940.2609610173115779No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA15820.23012281459694325No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT14840.21586741900244236No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT12890.18750209103379256No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTA9700.1410993237414886No Hit
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT9640.1402265444193763No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA8670.12611661204522742No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT8150.11855252458692082No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT7650.11127936356931832No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA7040.10240610712784326No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT65300.0285.670751
CGGGTTA66950.0279.069462
GGGGGGA87700.0269.527951
GGGTTAT70550.0264.82923
GGAGCAG91450.0257.671975
CAGAAGC92300.0255.617559
GAGCAGA92100.0255.534216
GGGGAGC94150.0252.46843
ATTAGTA74750.0249.35928
TATTAGT74850.0249.222447
TTATTAG75800.0245.905016
GGTTATT76800.0243.277344
GGGAGCA100200.0236.931144
TTAGTAG83500.0223.052699
GTTATTA85800.0217.416085
GGGGGAG114500.0207.854142
GCAGAAG116400.0203.703618
AGCAGAA141700.0166.503177
GGGTAGC806.2245796E-573.53
CGCAGAA1452.7054557E-760.827597
Produced by FastQC (version 0.11.2)