##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE77A l01n02 clinical flu pool f1.3420000000b62e.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	600706
Sequences flagged as poor quality	0
Sequence length	300
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83276844246603	34.0	34.0	34.0	32.0	34.0
2	33.2217440811312	34.0	34.0	34.0	34.0	34.0
3	33.218005147276706	34.0	34.0	34.0	34.0	34.0
4	33.216258868731124	34.0	34.0	34.0	34.0	34.0
5	33.25377139565778	34.0	34.0	34.0	34.0	34.0
6	36.570229030507434	38.0	38.0	38.0	34.0	38.0
7	36.693161047167834	38.0	38.0	38.0	35.0	38.0
8	36.710104443771165	38.0	38.0	38.0	35.0	38.0
9	36.61971913048979	38.0	38.0	38.0	35.0	38.0
10-14	36.66760977915986	38.0	38.0	38.0	34.8	38.0
15-19	36.684822525495	38.0	38.0	38.0	34.8	38.0
20-24	36.67262487806015	38.0	38.0	38.0	35.0	38.0
25-29	36.52669192583394	38.0	38.0	38.0	34.0	38.0
30-34	36.56159419083545	38.0	37.8	38.0	34.4	38.0
35-39	36.43988540151089	38.0	37.4	38.0	34.0	38.0
40-44	36.275226816445986	38.0	37.0	38.0	34.0	38.0
45-49	35.78956361348147	38.0	37.0	38.0	31.0	38.0
50-54	35.722057379150534	38.0	37.0	38.0	31.0	38.0
55-59	35.5713826730547	38.0	37.0	38.0	29.6	38.0
60-64	35.25449987181749	38.0	37.0	38.0	27.6	38.0
65-69	35.099659733713324	38.0	36.8	38.0	26.2	38.0
70-74	35.63458497168332	38.0	37.0	38.0	30.4	38.0
75-79	35.73383252373041	38.0	37.0	38.0	30.8	38.0
80-84	34.679983552686345	38.0	36.0	38.0	24.4	38.0
85-89	34.45325999740306	38.0	36.0	38.0	23.0	38.0
90-94	34.39172373840115	38.0	36.2	38.0	23.2	38.0
95-99	34.192972935179604	38.0	36.0	38.0	22.4	38.0
100-104	34.19819012961416	38.0	36.0	38.0	22.2	38.0
105-109	33.969210229296856	38.0	35.4	38.0	20.8	38.0
110-114	33.78625417425496	38.0	34.8	38.0	20.0	38.0
115-119	33.82860367634083	38.0	35.0	38.0	20.0	38.0
120-124	33.57150985673525	38.0	34.0	38.0	19.2	38.0
125-129	33.462126897350785	38.0	34.0	38.0	18.8	38.0
130-134	33.347619301288816	38.0	34.0	38.0	18.2	38.0
135-139	32.980754645367284	37.4	33.0	38.0	17.2	38.0
140-144	32.85020292788818	37.0	32.8	38.0	16.0	38.0
145-149	32.584985333923754	37.0	31.2	38.0	12.8	38.0
150-154	32.29936940866247	37.0	30.8	38.0	11.0	38.0
155-159	32.07844702733117	37.0	29.8	38.0	11.0	38.0
160-164	31.73146564209447	37.0	28.4	38.0	11.0	38.0
165-169	31.088230515426847	36.8	27.0	38.0	11.0	38.0
170-174	30.39543237457259	35.6	24.8	38.0	11.0	38.0
175-179	30.548141353673845	35.8	24.4	38.0	11.0	38.0
180-184	30.559917830020016	36.0	24.4	38.0	11.0	38.0
185-189	30.174297243576728	35.0	23.8	38.0	10.4	38.0
190-194	29.513023675475193	35.0	22.4	37.6	10.0	38.0
195-199	29.003957343525787	34.4	21.6	37.2	10.0	38.0
200-204	28.29066831361764	33.4	20.6	37.0	10.0	37.6
205-209	27.590831787929538	32.4	19.8	37.0	9.6	37.2
210-214	27.007573421940187	30.8	18.6	37.0	9.0	37.0
215-219	26.069834161802948	29.6	17.0	36.6	9.0	37.0
220-224	24.90116862491801	27.4	15.2	35.6	8.8	37.0
225-229	23.968327268247698	25.6	14.0	34.2	8.2	37.0
230-234	23.070473742562918	24.2	12.6	33.0	8.0	37.0
235-239	21.937367697342793	22.4	10.6	31.6	8.0	36.4
240-244	21.1575672625211	21.0	9.8	31.0	8.0	36.0
245-249	20.221219032272025	19.4	9.2	29.6	7.6	35.6
250-254	19.215610298548704	18.2	8.8	28.2	7.0	34.4
255-259	18.277637646369442	16.6	8.0	26.2	7.0	33.2
260-264	17.712391086488232	15.8	8.0	25.4	7.0	32.4
265-269	17.129714702366883	14.8	8.0	24.2	7.0	31.6
270-274	16.303529180664086	13.6	8.0	22.4	7.0	30.2
275-279	15.41509290734569	13.0	8.0	21.0	7.0	29.0
280-284	14.7283369901416	12.2	8.0	19.6	7.0	27.4
285-289	14.317150153319592	12.0	8.0	19.2	7.0	26.6
290-294	13.896437525178706	11.4	8.0	19.0	7.0	25.6
295-299	13.325378471332067	11.0	8.0	17.8	7.0	24.8
300	11.901782236235363	10.0	8.0	13.0	7.0	20.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.06590134042019713
1101	2	0.033775502847952055
1101	3	-0.10185954517640283
1101	4	-0.06880160882445807
1101	5	-0.015146900407700059
1101	6	0.06473793305413977
1101	7	-0.10971868858577238
1101	8	-0.0769355002067087
1101	9	0.01143098391028019
1101	10-14	0.060020092668473524
1101	15-19	0.06663894269112092
1101	20-24	0.05719572420811403
1101	25-29	0.008120464282356465
1101	30-34	0.016607618696390603
1101	35-39	0.01981794403781123
1101	40-44	0.08645476915759076
1101	45-49	0.1474380572544547
1101	50-54	0.1319182329898183
1101	55-59	0.17118325573792958
1101	60-64	0.2788313354078866
1101	65-69	0.2839532543253256
1101	70-74	0.2496975723143109
1101	75-79	0.22037565663291758
1101	80-84	0.2538767620576934
1101	85-89	0.3004667280742197
1101	90-94	0.27061125330480706
1101	95-99	0.15571945701729817
1101	100-104	0.19541819731787768
1101	105-109	0.29672206413504654
1101	110-114	0.18632809645183102
1101	115-119	0.22373231508159108
1101	120-124	0.20272361329843136
1101	125-129	0.18275875988656054
1101	130-134	0.13419019781579777
1101	135-139	0.1413388094606134
1101	140-144	0.17125508587968596
1101	145-149	0.21187924085405285
1101	150-154	0.014312911237240655
1101	155-159	0.23767320257675095
1101	160-164	0.23532836566612048
1101	165-169	0.4446807965940778
1101	170-174	1.0909648027203822
1101	175-179	0.057575265959993516
1101	180-184	-0.11656443616345058
1101	185-189	-0.04345839921243666
1101	190-194	-0.5131937635932289
1101	195-199	-0.6404334333395916
1101	200-204	-0.5390052039336517
1101	205-209	-0.2230757912553898
1101	210-214	-0.9368223836964233
1101	215-219	-0.4877097801039163
1101	220-224	-1.004389675863898
1101	225-229	-0.8411791631379231
1101	230-234	-0.5096739717007672
1101	235-239	-0.6230716393983791
1101	240-244	-0.6595529302239918
1101	245-249	-0.3974949611021614
1101	250-254	-0.24230852069456432
1101	255-259	-0.23192891618742806
1101	260-264	-0.2035551106823199
1101	265-269	-0.5128230218511618
1101	270-274	-0.42428633030043983
1101	275-279	-0.1790005025079715
1101	280-284	-0.12978068451136515
1101	285-289	-0.44681097885367294
1101	290-294	-0.13918283454479585
1101	295-299	-0.286805331151875
1101	300	0.8298437999891046
1102	1	0.00996469567670033
1102	2	0.00408222999784158
1102	3	-0.0339649404085236
1102	4	0.16111249213104628
1102	5	0.07801321927186677
1102	6	0.21219705862117166
1102	7	1.4522416396545168E-4
1102	8	0.18508978692774036
1102	9	0.09965567264688957
1102	10-14	0.150835496587014
1102	15-19	0.05726162389950673
1102	20-24	0.04209563734391253
1102	25-29	0.12244850211654068
1102	30-34	0.09777947469485326
1102	35-39	0.09951619710219006
1102	40-44	0.22736997039299922
1102	45-49	0.13112295253482387
1102	50-54	0.13343160042539637
1102	55-59	0.3407214856385252
1102	60-64	0.3145112890803148
1102	65-69	0.47865716321443585
1102	70-74	0.3568692352812022
1102	75-79	0.3677483040607683
1102	80-84	0.3683210821040319
1102	85-89	0.31477438177559947
1102	90-94	0.3432494156443653
1102	95-99	0.4561108478096898
1102	100-104	0.24832305206162886
1102	105-109	0.377759570167278
1102	110-114	0.4004863437252624
1102	115-119	0.49587917349293065
1102	120-124	0.3150456671542301
1102	125-129	0.40673652265077465
1102	130-134	0.33298444547529016
1102	135-139	0.2922725031195199
1102	140-144	0.4547386106359781
1102	145-149	0.47092635985801223
1102	150-154	0.3586317993754591
1102	155-159	0.5788180341567184
1102	160-164	0.32429815622688096
1102	165-169	0.7567014895773809
1102	170-174	1.446867128750693
1102	175-179	0.9153627382117087
1102	180-184	0.6306475317933078
1102	185-189	0.6651917310838655
1102	190-194	0.6335201829370263
1102	195-199	0.36752371365394154
1102	200-204	0.876567346978419
1102	205-209	0.6428730553810382
1102	210-214	0.8227985795310673
1102	215-219	0.6634927679126825
1102	220-224	0.3602698743725661
1102	225-229	0.538637534092075
1102	230-234	0.199866034090185
1102	235-239	0.5316639266666705
1102	240-244	0.795323857730839
1102	245-249	0.6346085038143556
1102	250-254	1.1383585191664558
1102	255-259	1.0369055206429962
1102	260-264	0.8697297845980607
1102	265-269	0.8697110192645638
1102	270-274	0.7716526717263896
1102	275-279	0.946578881721349
1102	280-284	1.0274600451479365
1102	285-289	0.5008390163205387
1102	290-294	0.36685596479889426
1102	295-299	0.4846025252326438
1102	300	0.9719748684187941
1103	1	0.07360934067952485
1103	2	-0.009374990494322333
1103	3	0.116058263042774
1103	4	0.01447839883910973
1103	5	0.1751364600674421
1103	6	0.20875326019275775
1103	7	0.09209182966310436
1103	8	0.1534846549805735
1103	9	0.08554766175298312
1103	10-14	0.1115654131014665
1103	15-19	-0.13714126614113553
1103	20-24	-0.1093957117532014
1103	25-29	-0.05016220647154768
1103	30-34	-0.07807018569099
1103	35-39	-0.0652834641430502
1103	40-44	-0.1545455565441003
1103	45-49	-0.21494332027213403
1103	50-54	-0.23550968156138907
1103	55-59	-0.20310600568557646
1103	60-64	-0.31328285177479387
1103	65-69	-0.06774395805133793
1103	70-74	-0.020302006189382382
1103	75-79	-0.04669283651776368
1103	80-84	0.2311085275524789
1103	85-89	-0.07754472896189668
1103	90-94	-0.08710538622004549
1103	95-99	-0.09007948924192277
1103	100-104	-0.09454130071832623
1103	105-109	-0.1786826298011519
1103	110-114	-0.06168546806583919
1103	115-119	-0.08735434072835346
1103	120-124	-0.05706456564591633
1103	125-129	0.06417904918630057
1103	130-134	0.03908078120501557
1103	135-139	-0.1101339369710459
1103	140-144	0.10432024537772833
1103	145-149	0.2177584246839288
1103	150-154	-0.002076398083566744
1103	155-159	-0.02016007647296192
1103	160-164	0.11248939641618705
1103	165-169	0.5744895522220084
1103	170-174	1.4284584323783953
1103	175-179	0.9320329726368826
1103	180-184	0.5363713622846795
1103	185-189	0.5530140598353626
1103	190-194	0.358082872099839
1103	195-199	0.47375911383926095
1103	200-204	0.653370770776565
1103	205-209	0.8164398711643805
1103	210-214	0.5883148133531009
1103	215-219	0.49491024097089564
1103	220-224	0.5478032928112597
1103	225-229	0.667524659067265
1103	230-234	0.5832904884965693
1103	235-239	0.6843184171204406
1103	240-244	0.5133028605605716
1103	245-249	0.82130722301509
1103	250-254	0.8184893335060295
1103	255-259	1.1590267692230505
1103	260-264	0.7008739725631976
1103	265-269	0.4884630017143188
1103	270-274	0.8124390265345021
1103	275-279	0.7695934241133511
1103	280-284	1.0093024460566973
1103	285-289	0.8572007464073632
1103	290-294	0.5530417080359076
1103	295-299	0.23318539592926335
1103	300	1.2073109903330081
2116	1	0.19133321113764623
2116	2	0.040232313400643704
2116	3	-0.007949280886300869
2116	4	0.08775080004510016
2116	5	0.06937449337982571
2116	6	0.1211033058497506
2116	7	0.13787244185723324
2116	8	-2.722691538039612E-4
2116	9	0.1108369694428788
2116	10-14	0.15969454777697933
2116	15-19	0.11575556129071884
2116	20-24	0.03809345953976617
2116	25-29	0.09916319512250737
2116	30-34	0.13266575306356998
2116	35-39	0.007329532142392736
2116	40-44	0.1448992115007428
2116	45-49	0.03957304850782606
2116	50-54	0.13024833558544202
2116	55-59	0.06711108893730255
2116	60-64	-0.09541669069016478
2116	65-69	0.1953255393236688
2116	70-74	0.1849105456961837
2116	75-79	0.15430067199862663
2116	80-84	0.1934598361440294
2116	85-89	0.16282149144237934
2116	90-94	0.17093104905914913
2116	95-99	0.2704319150571024
2116	100-104	0.1898655142296093
2116	105-109	0.23723899477662513
2116	110-114	0.2897415331687725
2116	115-119	0.19941278409014274
2116	120-124	0.31668989385254065
2116	125-129	0.3591919119833875
2116	130-134	0.30749241520504
2116	135-139	0.1860167915891111
2116	140-144	0.237207750056335
2116	145-149	0.4600374934189446
2116	150-154	0.5138452264942046
2116	155-159	0.42412422616902035
2116	160-164	0.3735277888612387
2116	165-169	0.8350425950906022
2116	170-174	1.5895825512901922
2116	175-179	1.0589546806448844
2116	180-184	0.7363508491658486
2116	185-189	0.9313978250795572
2116	190-194	0.7945104206060982
2116	195-199	0.513767502786358
2116	200-204	0.9352964933856249
2116	205-209	0.9291409360861103
2116	210-214	0.7858916629207577
2116	215-219	1.122131532363575
2116	220-224	1.5060543504232733
2116	225-229	0.37614828237292386
2116	230-234	0.6366786357409602
2116	235-239	0.522228497710973
2116	240-244	0.8618907083746592
2116	245-249	0.7017903681966899
2116	250-254	1.2188734186936472
2116	255-259	1.474396912378598
2116	260-264	1.1621995486718468
2116	265-269	0.82322554322851
2116	270-274	1.1297860067049008
2116	275-279	1.1866487812138349
2116	280-284	1.3656634647014378
2116	285-289	1.035455845409956
2116	290-294	0.757752913173988
2116	295-299	0.6402670909558239
2116	300	0.8455839541189327
1117	1	0.19933246026187845
1117	2	0.0025238923894406184
1117	3	0.15005178487285775
1117	4	0.23547463217592224
1117	5	0.13647985215008873
1117	6	0.1630425939105251
1117	7	0.17820287261165646
1117	8	0.07739606478003225
1117	9	-0.011578110443643652
1117	10-14	0.16132232836381633
1117	15-19	0.03914117838646547
1117	20-24	-0.0025704751098132306
1117	25-29	0.08135737220202088
1117	30-34	0.09664869486161365
1117	35-39	0.10507614790292052
1117	40-44	0.09381649708483764
1117	45-49	0.16600221034273943
1117	50-54	0.229737482705616
1117	55-59	0.22091398707555499
1117	60-64	0.10724929675688344
1117	65-69	0.42853370146439573
1117	70-74	0.37673220664549234
1117	75-79	0.34165151870188737
1117	80-84	0.24633314705202736
1117	85-89	0.16806180196584108
1117	90-94	0.33556805512368726
1117	95-99	0.4075553039290085
1117	100-104	0.3879164163402393
1117	105-109	0.3891416927822604
1117	110-114	0.35675492479590787
1117	115-119	0.288685479158886
1117	120-124	0.4662632874174122
1117	125-129	0.4501673995227904
1117	130-134	0.3572928124425445
1117	135-139	0.39020239415177116
1117	140-144	0.42079172854736413
1117	145-149	0.6604157319491648
1117	150-154	0.60121590479541
1117	155-159	0.7579454269041435
1117	160-164	0.7870369674092004
1117	165-169	1.1745703759802097
1117	170-174	1.667488485933724
1117	175-179	1.071724186005472
1117	180-184	0.7691827407975218
1117	185-189	0.9919815401585375
1117	190-194	1.1736182106394324
1117	195-199	1.1858951001958395
1117	200-204	1.0758689150508118
1117	205-209	0.8808476645991057
1117	210-214	0.8920397610553792
1117	215-219	1.2475082570313596
1117	220-224	1.4061114230372027
1117	225-229	1.2348701737321015
1117	230-234	0.48733474372174257
1117	235-239	0.9459544310828605
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2108	220-224	-0.7555059303844729
2108	225-229	-0.759025238790354
2108	230-234	-0.8349281990490205
2108	235-239	-0.964103874761495
2108	240-244	-0.9239839340359346
2108	245-249	-0.05149711142959745
2108	250-254	-0.7325543226689533
2108	255-259	0.35549221566674305
2108	260-264	-0.11944595224228394
2108	265-269	-0.6488782266209725
2108	270-274	-0.3035939248659787
2108	275-279	-0.0765483472934303
2108	280-284	-0.040221970798139495
2108	285-289	-0.32821322692326405
2108	290-294	-0.14700924447539876
2108	295-299	1.061705352065795
2108	300	1.552502386637526
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	16.0
10	95.0
11	200.0
12	510.0
13	873.0
14	1191.0
15	1842.0
16	4941.0
17	16013.0
18	25808.0
19	11409.0
20	6572.0
21	6549.0
22	8137.0
23	10227.0
24	12924.0
25	16425.0
26	20722.0
27	26979.0
28	35140.0
29	46390.0
30	59750.0
31	71514.0
32	78774.0
33	71961.0
34	47080.0
35	16884.0
36	1774.0
37	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.626372967807875	23.196205797844534	12.823078178010542	24.354343056337044
2	38.92752860800458	29.075621019267327	19.088705623050213	12.90814474967788
3	39.20287128811765	19.51586966003336	26.534277999553858	14.746981052295133
4	39.05687640875903	19.054912053483733	18.51071905391323	23.37749248384401
5	37.52601106031903	19.211228121576944	27.971753237024434	15.291007581079597
6	37.00046278878519	19.358055354865776	28.096106914197627	15.545374942151401
7	15.807732900953212	49.73947321984465	18.869297127047176	15.583496752154966
8	28.74800651233715	27.24510825595216	28.279724191201687	15.727161040509003
9	23.076846244252597	19.4685919567975	28.727364134867972	28.72719766408193
10-14	26.74729401737289	33.68523037892081	22.66313304678162	16.904342556924686
15-19	25.0047777115594	29.692528458180874	23.78764320649369	21.515050623766037
20-24	27.31888810832587	31.455087846633795	24.074805312415723	17.151218732624613
25-29	22.06007597726675	29.203637053733438	31.716413686562145	17.019873282437665
30-34	22.387457425096468	26.180427696743497	32.01486251177781	19.417252366382222
35-39	21.50526214154678	30.294320349721826	26.603363375761184	21.597054132970207
40-44	22.586722955988453	27.117591633844178	28.53029601835174	21.76538939181563
45-49	21.680322820148294	29.87637879428539	26.251244369125665	22.192054016440654
50-54	24.00841676294227	26.609089970801026	28.36226040692119	21.020232859335515
55-59	25.59145405572776	24.111329002873287	28.520540830289693	21.77667611110926
60-64	22.448152673687293	25.507253132147838	28.40244645467167	23.642147739493197
65-69	19.525458377309366	25.045196818410336	31.75736549992842	23.67197930435188
70-74	19.80932436166777	30.827259924155907	26.434462116243218	22.9289535979331
75-79	19.016923420109006	31.187103175263776	28.28312019523694	21.512853209390283
80-84	21.752937377019705	29.754122649016324	25.424916681371585	23.068023292592382
85-89	19.870285963516263	30.406088835470264	25.53335575139919	24.190269449614288
90-94	19.343072984122017	30.090626695921134	28.394522445256083	22.17177787470077
95-99	22.4999916764607	28.636570968160797	26.452907079336647	22.410530276041857
100-104	21.81892639660666	28.568251357569256	27.021304931197626	22.591517314626454
105-109	22.500690853762073	28.340519322264136	24.698105229513274	24.46068459446052
110-114	21.47076939467893	28.02176106115138	27.905164922607735	22.60230462156196
115-119	21.362363618808537	29.597007521150115	26.17619933877804	22.864429521263315
120-124	20.158247129211297	28.13143201499569	28.06774029225611	23.642580563536907
125-129	20.810046844879192	26.08810299880474	29.421713783448144	23.680136372867924
130-134	23.544029858200187	28.031915779099926	26.72368845991217	21.70036590278772
135-139	22.570675172214028	28.479921958495503	25.609133253205396	23.340269616085074
140-144	20.914390733570166	29.103088698964218	26.740568597616804	23.241951969848813
145-149	20.63115733819872	28.00211750839845	28.3012988050727	23.06542634833013
150-154	22.17674608768552	26.601341288863868	26.49476665615681	24.727145967293808
155-159	20.949549363582186	26.376829930115562	29.19554657353181	23.47807413277044
160-164	22.769507879062303	28.801510222970972	26.77316357752378	21.655818320442947
165-169	19.779326326023046	27.153582617786405	28.045133559511644	25.02195749667891
170-174	21.11282391053194	29.236431798583666	25.794481826384285	23.856262464500105
175-179	20.394735527862213	28.84236215386562	27.669408995415395	23.093493322856773
180-184	21.020998624951307	27.810110103777884	27.496345966246384	23.67254530502442
185-189	22.26869716633428	27.142928487479733	27.269412990714258	23.31896135547173
190-194	20.171131968050926	28.277426894354306	27.89770703139306	23.6537341062017
195-199	21.509024381311324	28.818889773033728	27.024334699503584	22.647751146151364
200-204	23.02933548191628	27.404420798194124	27.08965783594637	22.476585883943226
205-209	21.82358757861583	27.769191584568826	27.88898396220447	22.518236874610874
210-214	23.002200743791473	27.392201842498658	27.938825315545373	21.666772098164493
215-219	23.165375408269604	26.328719872949495	28.628713547059625	21.877191171721275
220-224	20.435960498466972	27.27158619077758	27.841648940296565	24.450804370458883
225-229	22.92206170739097	27.93443048679387	26.51023961804943	22.63326818776573
230-234	21.913182155663502	27.21481057289256	27.583743128918307	23.288264142525627
235-239	23.00326615682214	26.84854154944349	25.841193528947603	24.306998764786766
240-244	23.78204978808269	29.371106664491446	24.631650091725405	22.21519345570046
245-249	22.95192656640686	28.065409701251525	25.264638608570582	23.71802512377103
250-254	22.769241525804638	27.387773719589948	25.951064247735168	23.89192050687025
255-259	22.702819682173974	26.86445615658908	27.421833642414096	23.01089051882285
260-264	22.73687960499812	28.451322277453528	26.27002227379117	22.54177584375718
265-269	22.976064830382917	27.931367424330706	26.149364248068107	22.943203497218274
270-274	21.937120654696308	29.362117242045194	25.141217167799223	23.559544935459275
275-279	22.196881669235864	28.499299157990766	24.2449384557504	25.05888071702297
280-284	20.367933731309492	29.27834914250898	26.095494301704992	24.258222824476533
285-289	23.36701260417329	27.86145897043112	25.98936118146354	22.782167243932054
290-294	21.848757961465342	29.778593854564463	25.42891198023659	22.943736203733607
295-299	21.518246862858035	30.50690354349715	23.36773729578196	24.607112297862848
300	13.719356890059364	39.93367803884097	21.73509170875603	24.61187336234364
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.25
5	0.5
6	0.5
7	0.25
8	0.0
9	0.0
10	1.0
11	1.5
12	0.5
13	1.5
14	1.0
15	2.25
16	3.5
17	3.5
18	3.5
19	4.0
20	6.75
21	7.0
22	10.5
23	12.0
24	24.25
25	34.5
26	43.25
27	59.0
28	63.25
29	83.0
30	109.5
31	156.0
32	238.25
33	392.75
34	591.25
35	754.0
36	1165.25
37	2151.0
38	4356.25
39	7278.25
40	10621.25
41	13789.5
42	18781.0
43	21386.75
44	20926.5
45	19218.0
46	14735.25
47	11748.25
48	10461.0
49	9323.25
50	8268.75
51	5558.25
52	3039.0
53	2085.0
54	1730.25
55	1661.5
56	2018.75
57	2118.75
58	1502.25
59	973.75
60	628.5
61	421.25
62	308.0
63	253.5
64	229.75
65	197.5
66	150.75
67	107.5
68	73.0
69	45.25
70	35.75
71	24.75
72	20.5
73	19.5
74	16.5
75	11.75
76	8.25
77	7.75
78	7.0
79	3.75
80	3.75
81	4.75
82	5.5
83	2.25
84	3.0
85	1.75
86	1.25
87	0.25
88	0.75
89	0.75
90	0.5
91	1.0
92	1.0
93	0.25
94	0.25
95	0.5
96	0.0
97	0.0
98	0.0
99	0.25
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	3.3294157208351504E-5
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	3.3294157208351504E-5
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	3.3294157208351504E-5
290-294	0.0
295-299	0.0
300	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	600706.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.39642448731273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.48022056789553	16.009013622853306
2	5.728440975962586	2.5659319149634823
3	3.035121820355658	2.039276299781719
4	2.279859537757698	2.0424280791628
5	2.0045242578039595	2.2447088086446487
6	1.927959458128354	2.590763905114323
7	1.471503283770545	2.306948852445981
8	1.424759632271865	2.5527617213398623
9	1.1357663828814388	2.2893395426489214
>10	9.194903143617058	36.33159038013432
>50	0.2320545711850271	3.3620002470586323
>100	0.05681707928316352	2.5044038874435004
>500	0.0059093464812361605	0.9702769211127331
>1k	0.017727954084703332	9.849308019119556
>5k	0.0022159942605468716	3.4321040070371276
>10k+	0.0022159942605468716	8.909143791139035
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT	31339	5.217027963762639	Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	12316	2.0502542008902855	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	10198	1.6976690760538433	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	9316	1.5508418427650132	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	6127	1.0199665060778484	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	5303	0.8827945783794401	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	4709	0.7839109314706362	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	4533	0.7546120731272868	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	4355	0.7249802732118541	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	4324	0.7198196788445596	No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	4074	0.6782019823341202	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	3374	0.5616724321048899	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	2989	0.4975811794788133	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2868	0.4774382143677606	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	2836	0.4721111492144244	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2631	0.4379846380758641	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTTGGACAGTACGGATGACAA	2518	0.41917343925314543	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	2181	0.3630727843570732	No Hit
GGGGGGAGCGAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCA	2133	0.3550821866270688	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	2078	0.34592629339477216	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1998	0.3326086305114316	No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	1822	0.3033097721680822	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATAT	1440	0.23971793190013085	Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	1331	0.22157261622157928	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1313	0.21857614207282766	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT	1258	0.20942024884053095	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1258	0.20942024884053095	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATTT	1218	0.20276141739886067	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1213	0.20192906346865191	No Hit
GGGGGGAGCGAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAA	1082	0.18012139049718165	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	984	0.1638072534650894	No Hit
GGGGGGAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCA	885	0.1473266456469554	No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG	831	0.13833722320070052	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGAACT	819	0.1363395737681994	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGGTGCAGGATAAATGAAGGAACTGAACCAGCTAG	621	0.10337835813193143	No Hit
GGGGGGAGCGAAAGCAGGATCCCTGATGCCTATGACAGTGGCTGCCACAT	620	0.10321188734588967	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.005660006725419756	0.0	0.0
2	0.0068253022277120585	0.0	0.0
3	0.01015471794854721	1.6647078604175753E-4	0.0
4	0.013983546027507633	1.6647078604175753E-4	0.0
5	0.01831178646459333	1.6647078604175753E-4	0.0
6	0.02280649768772078	1.6647078604175753E-4	0.0
7	0.029465329129391083	1.6647078604175753E-4	0.0
8	0.03845475157564599	1.6647078604175753E-4	0.0
9	0.0442812290871075	1.6647078604175753E-4	0.0
10-14	0.06039560117594963	1.6647078604175753E-4	0.0
15-19	0.08792986918725633	1.6647078604175753E-4	0.0
20-24	0.12941438906886232	1.6647078604175753E-4	0.0
25-29	0.16523890222504853	1.6647078604175753E-4	0.0
30-34	0.2093869546833226	1.6647078604175753E-4	0.0
35-39	0.23242651147150184	1.6647078604175753E-4	0.0
40-44	0.25047194467842837	1.6647078604175753E-4	0.0
45-49	0.2710144396759812	1.6647078604175753E-4	0.0
50-54	0.29422046725020223	1.6647078604175753E-4	0.0
55-59	0.3227535599777595	1.6647078604175753E-4	0.0
60-64	0.3526517131508592	1.6647078604175753E-4	0.0
65-69	0.3915725829274221	1.6647078604175753E-4	0.0
70-74	0.433523221009945	1.6647078604175753E-4	0.0
75-79	0.4842302224382643	1.6647078604175753E-4	0.0
80-84	0.5385329928450856	1.6647078604175753E-4	0.0
85-89	0.6159419083545028	1.6647078604175753E-4	0.0
90-94	0.7267115693866883	1.6647078604175753E-4	0.0
95-99	0.8493006562278385	1.6647078604175753E-4	0.0
100-104	0.9583390210851898	1.6647078604175753E-4	0.0
105-109	1.0894847063288864	1.6647078604175753E-4	0.0
110-114	1.2191321544982072	1.6647078604175753E-4	0.0
115-119	1.351642900187446	1.6647078604175753E-4	0.0
120-124	1.507925674123448	1.6647078604175753E-4	0.0
125-129	1.6869816515899623	1.6647078604175753E-4	0.0
130-134	1.8856478876521963	1.6647078604175753E-4	0.0
135-139	2.1110160377955274	1.6647078604175753E-4	0.0
140-144	2.3680802256012092	1.6647078604175753E-4	0.0
145-149	2.6654636377862047	1.6647078604175753E-4	0.0
150-154	2.9965740312232607	1.6647078604175753E-4	0.0
155-159	3.3599797571524173	1.6647078604175753E-4	0.0
160-164	3.7525511647960905	1.6647078604175753E-4	0.0
165-169	4.177884023132781	1.6647078604175753E-4	0.0
170-174	4.673101317449801	1.6647078604175753E-4	6.658831441670301E-5
175-179	5.25135423984445	1.6647078604175753E-4	1.6647078604175753E-4
180-184	6.022113979217787	1.6647078604175753E-4	1.6647078604175753E-4
185-189	7.060392271760229	1.6647078604175753E-4	1.6647078604175753E-4
190-194	7.847965560523784	1.6647078604175753E-4	1.6647078604175753E-4
195-199	8.708286582787586	1.6647078604175753E-4	1.6647078604175753E-4
200-204	9.61305530492454	1.6647078604175753E-4	2.3305910045846057E-4
205-209	10.558975605371014	1.6647078604175753E-4	3.3294157208351506E-4
210-214	11.568354569456606	1.6647078604175753E-4	3.3294157208351506E-4
215-219	12.601871797518253	1.6647078604175753E-4	3.3294157208351506E-4
220-224	13.668816359417084	1.6647078604175753E-4	3.3294157208351506E-4
225-229	14.763128718541186	1.6647078604175753E-4	3.3294157208351506E-4
230-234	15.858573079010364	1.6647078604175753E-4	3.3294157208351506E-4
235-239	16.950188611400584	1.6647078604175753E-4	3.9952988650021804E-4
240-244	17.978212303522856	1.6647078604175753E-4	4.994123581252726E-4
245-249	18.93045849383892	1.6647078604175753E-4	4.994123581252726E-4
250-254	19.829467326778822	1.6647078604175753E-4	4.994123581252726E-4
255-259	20.70120824496509	1.6647078604175753E-4	4.994123581252726E-4
260-264	21.55253984478264	1.6647078604175753E-4	4.994123581252726E-4
265-269	22.34567325779999	1.6647078604175753E-4	4.994123581252726E-4
270-274	23.003432627608184	1.6647078604175753E-4	5.660006725419755E-4
275-279	23.58831108728729	1.6647078604175753E-4	6.658831441670301E-4
280-284	24.131305497198294	1.6647078604175753E-4	6.658831441670301E-4
285-288	24.601385702823013	1.6647078604175753E-4	6.658831441670301E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	5545	0.0	289.49323	1
GGGTTAT	5615	0.0	285.88425	2
GGGGGGA	8660	0.0	269.21707	1
GTTATTA	6080	0.0	262.0855	4
GGTTATT	6125	0.0	261.6	3
GGGGAGC	8985	0.0	261.1152	3
TTATTAG	6135	0.0	259.73593	5
GGAGCGA	6240	0.0	255.12979	5
GGGAGCG	6245	0.0	254.45477	4
TTAGTAG	6415	0.0	248.39906	8
TATTAGT	6615	0.0	241.33333	6
GGGGGAG	9755	0.0	241.2578	2
ATTAGTA	6665	0.0	239.52287	7
GAGCGAA	6880	0.0	231.3968	6
CGAAAGC	7000	0.0	228.90001	9
TAGTAGA	7045	0.0	226.81194	9
AGCGAAA	7055	0.0	226.28207	7
GCGAAAG	7185	0.0	222.59709	8
GGGAGCA	3570	0.0	210.0	4
GCAAAAG	3925	0.0	187.26115	8
>>END_MODULE