FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n02 clinical flu pool f1.3420000000b62e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n02 clinical flu pool f1.3420000000b62e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600706
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT313395.217027963762639Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC123162.0502542008902855No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG101981.6976690760538433No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA93161.5508418427650132No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT61271.0199665060778484No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT53030.8827945783794401No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT47090.7839109314706362No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG45330.7546120731272868No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT43550.7249802732118541No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT43240.7198196788445596No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA40740.6782019823341202No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT33740.5616724321048899No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG29890.4975811794788133No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT28680.4774382143677606No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA28360.4721111492144244No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA26310.4379846380758641No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTTGGACAGTACGGATGACAA25180.41917343925314543No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA21810.3630727843570732No Hit
GGGGGGAGCGAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCA21330.3550821866270688No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG20780.34592629339477216No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT19980.3326086305114316No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA18220.3033097721680822No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATAT14400.23971793190013085Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA13310.22157261622157928No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT13130.21857614207282766No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT12580.20942024884053095No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG12580.20942024884053095No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATTT12180.20276141739886067Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA12130.20192906346865191No Hit
GGGGGGAGCGAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAA10820.18012139049718165No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA9840.1638072534650894No Hit
GGGGGGAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCA8850.1473266456469554No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG8310.13833722320070052No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGAACT8190.1363395737681994Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGGTGCAGGATAAATGAAGGAACTGAACCAGCTAG6210.10337835813193143No Hit
GGGGGGAGCGAAAGCAGGATCCCTGATGCCTATGACAGTGGCTGCCACAT6200.10321188734588967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA55450.0289.493231
GGGTTAT56150.0285.884252
GGGGGGA86600.0269.217071
GTTATTA60800.0262.08554
GGTTATT61250.0261.63
GGGGAGC89850.0261.11523
TTATTAG61350.0259.735935
GGAGCGA62400.0255.129795
GGGAGCG62450.0254.454774
TTAGTAG64150.0248.399068
TATTAGT66150.0241.333336
GGGGGAG97550.0241.25782
ATTAGTA66650.0239.522877
GAGCGAA68800.0231.39686
CGAAAGC70000.0228.900019
TAGTAGA70450.0226.811949
AGCGAAA70550.0226.282077
GCGAAAG71850.0222.597098
GGGAGCA35700.0210.04
GCAAAAG39250.0187.261158
Produced by FastQC (version 0.11.2)