##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE77A l01n02 clinical flu pool e4.3420000000b77d.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1946342
Sequences flagged as poor quality	0
Sequence length	300
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.04126715654289	34.0	34.0	34.0	34.0	34.0
2	33.38686417906	34.0	34.0	34.0	34.0	34.0
3	33.410136039812116	34.0	34.0	34.0	34.0	34.0
4	33.400017571423724	34.0	34.0	34.0	34.0	34.0
5	33.39217773649235	34.0	34.0	34.0	34.0	34.0
6	36.88874514345372	38.0	38.0	38.0	36.0	38.0
7	36.955666064853965	38.0	38.0	38.0	36.0	38.0
8	36.912696227076225	38.0	38.0	38.0	36.0	38.0
9	36.89346579378136	38.0	38.0	38.0	36.0	38.0
10-14	36.94869401163824	38.0	38.0	38.0	36.0	38.0
15-19	36.98979675719889	38.0	38.0	38.0	36.0	38.0
20-24	36.95887536722734	38.0	38.0	38.0	36.0	38.0
25-29	36.86926244205797	38.0	38.0	38.0	36.0	38.0
30-34	36.956946004350726	38.0	38.0	38.0	36.0	38.0
35-39	36.85657279142104	38.0	38.0	38.0	36.0	38.0
40-44	36.78366258345142	38.0	38.0	38.0	35.8	38.0
45-49	36.81412896602961	38.0	38.0	38.0	36.0	38.0
50-54	36.70873155899632	38.0	38.0	38.0	35.2	38.0
55-59	36.71978922512077	38.0	38.0	38.0	35.0	38.0
60-64	36.68652066286397	38.0	38.0	38.0	35.0	38.0
65-69	36.396423239081315	38.0	37.4	38.0	34.0	38.0
70-74	36.599672616631615	38.0	37.6	38.0	34.4	38.0
75-79	36.5068505946026	38.0	37.8	38.0	34.4	38.0
80-84	36.18248478427738	38.0	37.0	38.0	33.6	38.0
85-89	36.274245533416014	38.0	37.0	38.0	34.0	38.0
90-94	36.21525107098341	38.0	37.0	38.0	33.6	38.0
95-99	36.05705554316765	38.0	37.0	38.0	32.8	38.0
100-104	36.117266749625706	38.0	37.0	38.0	33.0	38.0
105-109	35.88632059524996	38.0	37.0	38.0	31.8	38.0
110-114	35.71999021754656	38.0	37.0	38.0	30.6	38.0
115-119	35.793830888918805	38.0	37.0	38.0	31.4	38.0
120-124	35.54059214670392	38.0	37.0	38.0	30.0	38.0
125-129	35.49822518344669	38.0	37.0	38.0	29.6	38.0
130-134	35.45244042413923	38.0	36.8	38.0	29.4	38.0
135-139	35.12331173041531	38.0	36.2	38.0	27.6	38.0
140-144	35.042313632444866	38.0	36.0	38.0	27.2	38.0
145-149	34.78630240728505	38.0	35.8	38.0	25.4	38.0
150-154	34.56821165036771	38.0	35.0	38.0	24.2	38.0
155-159	34.36483434052186	38.0	35.0	38.0	24.0	38.0
160-164	34.07805955993346	37.2	34.2	38.0	23.2	38.0
165-169	33.36305489990967	37.0	32.8	38.0	20.4	38.0
170-174	32.693580881468925	37.0	30.2	38.0	19.4	38.0
175-179	33.075975239706075	37.0	32.0	38.0	21.0	38.0
180-184	33.10819897017071	37.0	32.0	38.0	21.0	38.0
185-189	32.784960094371904	37.0	30.6	38.0	20.8	38.0
190-194	32.04083413911842	37.0	29.4	37.8	18.2	38.0
195-199	31.56159554692855	36.0	28.0	37.8	16.8	38.0
200-204	30.90374774834022	35.0	26.8	37.0	15.2	37.8
205-209	30.113887179128845	34.4	24.6	37.0	11.4	37.6
210-214	29.458446665591147	33.8	23.6	37.0	10.0	37.2
215-219	28.444126571794676	32.2	21.8	37.0	10.0	37.0
220-224	27.282078380880648	30.6	20.2	36.6	9.4	37.0
225-229	26.358284515259907	29.2	19.4	35.8	9.0	37.0
230-234	25.30294316209587	27.6	17.4	34.4	9.0	37.0
235-239	24.127344218025407	25.6	15.6	33.6	8.0	37.0
240-244	23.226442937572124	24.6	14.2	32.6	8.0	36.8
245-249	22.090113453853437	22.4	12.4	31.4	8.0	36.2
250-254	20.847192014558594	20.6	10.4	30.0	8.0	36.0
255-259	19.79943052146026	19.2	9.0	28.6	7.0	34.6
260-264	19.21030168387673	18.4	9.0	27.4	7.4	34.0
265-269	18.450867113796033	17.2	8.6	26.2	7.0	33.2
270-274	17.405320647655962	15.6	8.0	24.4	7.0	31.8
275-279	16.391317147757178	14.0	8.0	22.6	7.0	30.2
280-284	15.544385519091714	13.2	8.0	21.2	7.0	29.2
285-289	15.075128317633798	13.0	8.0	20.6	7.0	28.0
290-294	14.568638605137227	12.2	8.0	19.2	7.0	27.0
295-299	13.9299667787059	11.8	8.0	18.8	7.0	25.8
300	12.170523474291773	11.0	8.0	14.0	7.0	21.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.1029524783363982
1101	2	0.010693231256219349
1101	3	0.021713802885805933
1101	4	0.02671539059475947
1101	5	0.0214387054047549
1101	6	0.1380988431498551
1101	7	0.08940447957185427
1101	8	0.07307837619891444
1101	9	0.04109652208187953
1101	10-14	0.08597570261098042
1101	15-19	0.11196563480349653
1101	20-24	0.07265581003048993
1101	25-29	0.02528952732748735
1101	30-34	0.0684032517062434
1101	35-39	0.0721956839297988
1101	40-44	0.09238254373728694
1101	45-49	0.06208129658896411
1101	50-54	0.05579270134115433
1101	55-59	0.04741032078958085
1101	60-64	0.15351514191761595
1101	65-69	0.20264028602724693
1101	70-74	0.0955392285989447
1101	75-79	0.14912022455010998
1101	80-84	0.15376082182369544
1101	85-89	0.1319118773682746
1101	90-94	0.12273462463072349
1101	95-99	0.08674537512128211
1101	100-104	0.1569537318102192
1101	105-109	0.1699503526767785
1101	110-114	0.2432630514014278
1101	115-119	0.27857425828177185
1101	120-124	0.17544455866586617
1101	125-129	0.18879198885951354
1101	130-134	0.13024999676979832
1101	135-139	0.12470223761869192
1101	140-144	0.06325329559192028
1101	145-149	0.31445287936827526
1101	150-154	0.053495337098816265
1101	155-159	0.19908249196759442
1101	160-164	0.18409640533428728
1101	165-169	0.4618215939544612
1101	170-174	1.1902939835788544
1101	175-179	0.2907359546712911
1101	180-184	-0.13695630811287884
1101	185-189	-0.044168446835264774
1101	190-194	-0.6137131611939282
1101	195-199	-0.7035979426509833
1101	200-204	-0.5033689437147544
1101	205-209	-0.15601182840331163
1101	210-214	-0.9124224433143908
1101	215-219	-0.7049564493111333
1101	220-224	-0.9612803970667869
1101	225-229	-0.6185039690878327
1101	230-234	-0.5098054881927006
1101	235-239	-0.6208404921907729
1101	240-244	-0.6041495515818767
1101	245-249	-0.5262652989472869
1101	250-254	-0.07545482980794205
1101	255-259	0.007128852620652992
1101	260-264	-0.15267173253986854
1101	265-269	-0.4225216213279701
1101	270-274	-0.23306747820662466
1101	275-279	-0.17207837122855096
1101	280-284	-0.044661068238225354
1101	285-289	-0.10253041216051706
1101	290-294	-0.13735353966040087
1101	295-299	-0.10565689447497384
1101	300	0.520187357796722
1102	1	0.05917259784049378
1102	2	-0.005049175639378234
1102	3	-0.024986537485233384
1102	4	0.06312467878898076
1102	5	0.060909091196812426
1102	6	0.20152612749603804
1102	7	0.0985664138327067
1102	8	0.07212622376631828
1102	9	0.08218887849466938
1102	10-14	0.059335858366893035
1102	15-19	-0.004024421539945422
1102	20-24	-0.01909644471861327
1102	25-29	0.03115770567686127
1102	30-34	0.04505264339697845
1102	35-39	0.04867094333740596
1102	40-44	0.0814024178336652
1102	45-49	0.017933494374680947
1102	50-54	-0.011299685302169848
1102	55-59	0.10664590000121876
1102	60-64	0.12397116521002971
1102	65-69	0.12717525235488125
1102	70-74	0.06873659217469452
1102	75-79	0.18529735097094857
1102	80-84	0.14433569932612045
1102	85-89	0.09946899647389529
1102	90-94	0.06256350517855225
1102	95-99	0.18698562757987247
1102	100-104	0.09480614851176483
1102	105-109	0.1813258998659606
1102	110-114	0.15156310132563533
1102	115-119	0.19029252777183103
1102	120-124	0.09204430801528218
1102	125-129	0.20256971013554192
1102	130-134	0.25111903566722305
1102	135-139	0.17433314391928434
1102	140-144	0.12888378742744777
1102	145-149	0.1993412611370431
1102	150-154	0.1311765978378716
1102	155-159	0.22507761521217162
1102	160-164	0.28622734431160524
1102	165-169	0.49101500594609604
1102	170-174	1.3187922027153576
1102	175-179	0.6739719235209805
1102	180-184	0.5126504940795797
1102	185-189	0.506781255755385
1102	190-194	0.28246414339721326
1102	195-199	0.12264583042398769
1102	200-204	0.5157769909970682
1102	205-209	0.1965417210890834
1102	210-214	0.555376958223178
1102	215-219	0.6379731717315451
1102	220-224	0.29261619886504775
1102	225-229	0.5400858887417428
1102	230-234	0.14690261354050804
1102	235-239	0.29247626448720254
1102	240-244	0.4049040127702206
1102	245-249	0.4559786993232926
1102	250-254	1.0722454476923353
1102	255-259	0.847180942613317
1102	260-264	0.6031473649967722
1102	265-269	0.6000557569848048
1102	270-274	0.5301038323405258
1102	275-279	0.7261263363965007
1102	280-284	0.890018930923496
1102	285-289	0.600877153696235
1102	290-294	0.49557868164670715
1102	295-299	0.3106166060264215
1102	300	1.0607548054506104
1103	1	-0.10128192648740963
1103	2	0.0579372582246549
1103	3	5.722474135794187E-4
1103	4	-0.02860924991808389
1103	5	0.057595283014549636
1103	6	0.15736820062009826
1103	7	0.04869687133910361
1103	8	0.024017031282198786
1103	9	0.08450106167672544
1103	10-14	0.045583917410631614
1103	15-19	-0.1152957399542558
1103	20-24	-0.10594361815876852
1103	25-29	-0.06952502643947156
1103	30-34	-0.049387150348898956
1103	35-39	-0.07206896549593722
1103	40-44	-0.14165983088906842
1103	45-49	-0.2014051244838555
1103	50-54	-0.20818404236035093
1103	55-59	-0.13506894474891595
1103	60-64	-0.22722434564207816
1103	65-69	-0.008269766476985296
1103	70-74	-0.09280142371689948
1103	75-79	-0.02193107987103815
1103	80-84	0.2977890126563807
1103	85-89	0.15829641920429793
1103	90-94	0.07619222309344309
1103	95-99	0.06662506300995119
1103	100-104	0.004133070374940928
1103	105-109	-6.896213631932824E-4
1103	110-114	0.15160176540049264
1103	115-119	0.07157286245284666
1103	120-124	-0.01234762151760549
1103	125-129	0.16573496625786532
1103	130-134	0.20649865026542358
1103	135-139	0.13052096099259813
1103	140-144	0.1171085518585997
1103	145-149	0.3181617128055123
1103	150-154	0.21273438136706346
1103	155-159	0.12851420864669194
1103	160-164	0.4219068575835081
1103	165-169	0.704745648462179
1103	170-174	1.5738033812954484
1103	175-179	0.8899393619344522
1103	180-184	0.5551131993725775
1103	185-189	0.5735674656027356
1103	190-194	0.6197350054893462
1103	195-199	0.5753861385209937
1103	200-204	0.7006102980508899
1103	205-209	0.7992413914119894
1103	210-214	0.753044096802185
1103	215-219	0.6257241972707739
1103	220-224	0.5816982419305035
1103	225-229	0.7168096195163258
1103	230-234	0.8111112232616584
1103	235-239	0.555940034410348
1103	240-244	0.7246160438497391
1103	245-249	0.862556612030918
1103	250-254	1.047831977475969
1103	255-259	1.2157846777278465
1103	260-264	0.7914620513110258
1103	265-269	0.5559927938632079
1103	270-274	0.6660645740399183
1103	275-279	1.013335751138861
1103	280-284	1.2335250044082962
1103	285-289	0.8887510082666346
1103	290-294	0.6647600721948006
1103	295-299	0.6059531888737464
1103	300	1.186660545515224
2116	1	0.13724391239291123
2116	2	0.0021728053528917712
2116	3	0.014400810631244099
2116	4	0.026559793475065874
2116	5	0.015421656230309111
2116	6	0.08450359620039904
2116	7	0.02138158016282432
2116	8	-0.04152324469608004
2116	9	0.03119591354154494
2116	10-14	0.05318241551941583
2116	15-19	-0.044143782972703605
2116	20-24	-0.08865880180915298
2116	25-29	-0.02612607898400654
2116	30-34	0.008751665500348338
2116	35-39	-0.06565086568416234
2116	40-44	0.03467396751715057
2116	45-49	-0.016831910360664892
2116	50-54	-0.05467018091166409
2116	55-59	0.018300878732986803
2116	60-64	-0.14013991511723134
2116	65-69	0.06569059097407859
2116	70-74	-0.05501558187960853
2116	75-79	0.0034758058712682782
2116	80-84	0.09579975851929845
2116	85-89	0.09890868614372295
2116	90-94	0.037578275036132425
2116	95-99	0.050948955063624624
2116	100-104	0.10354308094131426
2116	105-109	0.15894892708149655
2116	110-114	0.13643444745461863
2116	115-119	0.08391496658084918
2116	120-124	0.15146959720962627
2116	125-129	0.2502205954912782
2116	130-134	0.22117413619017157
2116	135-139	0.1725855649639385
2116	140-144	0.22925968165956334
2116	145-149	0.2945471661568462
2116	150-154	0.3490110534391917
2116	155-159	0.3628406443972665
2116	160-164	0.34701253128588405
2116	165-169	0.8287443891604624
2116	170-174	1.634313031126915
2116	175-179	0.9546516763236781
2116	180-184	0.7221643372981887
2116	185-189	0.7039087509025848
2116	190-194	0.8784694911529343
2116	195-199	0.3986697333898732
2116	200-204	0.7689793746947018
2116	205-209	0.7849715736177032
2116	210-214	0.8611771111423039
2116	215-219	1.1470591829163297
2116	220-224	1.2896095080924255
2116	225-229	0.4755401820652878
2116	230-234	0.8927867887695946
2116	235-239	0.7937013396063541
2116	240-244	0.8786652712366099
2116	245-249	0.8869375364085634
2116	250-254	1.2153681425363345
2116	255-259	1.3562230910293849
2116	260-264	1.0561416574100662
2116	265-269	0.8783607699377747
2116	270-274	0.9878858812672675
2116	275-279	1.070104000681635
2116	280-284	1.221978876733946
2116	285-289	1.1181265818413593
2116	290-294	0.7749410680707545
2116	295-299	0.6970365495634177
2116	300	0.961361568927467
1117	1	0.10401875832536689
1117	2	-0.05632091470547351
1117	3	-0.017735742723679948
1117	4	-0.05805509279293375
1117	5	-0.10312660286498954
1117	6	-0.012773909780278814
1117	7	0.05922389902393377
1117	8	0.1214498168814302
1117	9	-0.016908751174611325
1117	10-14	0.0413652185976332
1117	15-19	-0.051016231785460775
1117	20-24	-0.03147017860460721
1117	25-29	0.059166715714866314
1117	30-34	0.04588811220783384
1117	35-39	-0.020151998410476324
1117	40-44	0.09323928803222259
1117	45-49	0.08064667948405457
1117	50-54	0.08678260510186675
1117	55-59	0.09717966743809114
1117	60-64	0.07998917067073563
1117	65-69	0.306701472671854
1117	70-74	0.19034511809561394
1117	75-79	0.2561379490201148
1117	80-84	0.163576152596157
1117	85-89	0.08747205224009491
1117	90-94	0.11342931899881847
1117	95-99	0.2035497496722556
1117	100-104	0.2612799517254203
1117	105-109	0.2605683568931525
1117	110-114	0.25156868436890534
1117	115-119	0.18862710011693196
1117	120-124	0.2409384671800865
1117	125-129	0.26933313006524173
1117	130-134	0.3079676868909331
1117	135-139	0.25113699487452834
1117	140-144	0.3200442733948208
1117	145-149	0.4821669431068685
1117	150-154	0.4694573040460739
1117	155-159	0.6102062440715557
1117	160-164	0.7232791671569672
1117	165-169	1.104201920315738
1117	170-174	1.5559396680612139
1117	175-179	1.0890093828913763
1117	180-184	0.7662435368476679
1117	185-189	0.8963779342246383
1117	190-194	1.0915520809326082
1117	195-199	1.1003340456646278
1117	200-204	0.94493105836467
1117	205-209	0.8410313706456947
1117	210-214	0.5530492968672007
1117	215-219	1.0624226774531778
1117	220-224	1.3837608856968764
1117	225-229	1.2213818095467133
1117	230-234	0.7870312266884447
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2108	210-214	0.08040070698345403
2108	215-219	-0.3667829299968801
2108	220-224	-0.6017739109213984
2108	225-229	-0.7228208473742761
2108	230-234	-0.6128669297172387
2108	235-239	-0.5464763926423792
2108	240-244	-0.8470620254228365
2108	245-249	0.058750263186261975
2108	250-254	-0.34847048773528044
2108	255-259	0.33720987302461936
2108	260-264	-0.12305835484582417
2108	265-269	-0.5331898023550465
2108	270-274	-0.1697602179882871
2108	275-279	-0.1990174045047297
2108	280-284	0.18447397709842228
2108	285-289	-0.3006442217792209
2108	290-294	-0.21864975244448637
2108	295-299	1.0728191498204112
2108	300	1.453024162499288
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	49.0
11	285.0
12	1286.0
13	2628.0
14	2800.0
15	2760.0
16	3970.0
17	6297.0
18	14364.0
19	15783.0
20	12446.0
21	13631.0
22	17149.0
23	22589.0
24	30050.0
25	40906.0
26	56242.0
27	78234.0
28	109715.0
29	152512.0
30	206023.0
31	262870.0
32	301065.0
33	290850.0
34	205733.0
35	84294.0
36	11725.0
37	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.295270820852654	25.626020504104623	14.76246209556183	28.316246579480893
2	36.751197888140936	24.79939291244807	20.92443157471811	17.524977624692887
3	40.70949504249511	21.74818197418542	21.761797258652386	15.780525724667093
4	40.6904850226733	21.286598141539358	20.442604639883434	17.580312195903904
5	33.43913865086403	21.278017943403572	29.061593491791264	16.221249913941126
6	29.24285659971372	21.318709661508613	32.8744383052927	16.56399543348497
7	16.609002939873875	42.02950971617527	24.885092136942017	16.47639520700884
8	27.52337461761602	25.95314698033542	29.739583279814134	16.783895122234426
9	16.879561762526833	21.4416582491669	33.61356842733703	28.065211560969246
10-14	24.71795809780604	34.11480613376272	24.794727750826937	16.372508017604307
15-19	23.288281298970066	31.98364932781598	23.422142665574704	21.30592670763925
20-24	23.907936015355986	30.41375051249986	27.290075433813787	18.388238038330364
25-29	19.04383710570907	28.187348369402702	34.14916802905142	18.619646495836804
30-34	18.76983592811541	29.144518280959876	31.130448811154466	20.955196979770257
35-39	20.787826599847303	29.955362418321137	28.18647493605954	21.070336045772017
40-44	19.51500815375715	30.095132304600114	29.402653798767126	20.98720574287561
45-49	21.50532640204034	29.172581180491402	27.96379053629835	21.358301881169908
50-54	21.904834813203436	29.91244087626943	27.571290143253346	20.61143416727379
55-59	21.705003539973962	28.399870115324028	28.325885173314862	21.569241171387148
60-64	20.840756660443027	27.49176660627988	29.456529222510742	22.210947510766353
65-69	19.026409541591356	28.137819561002125	29.576097109346662	23.25967378805986
70-74	19.69474018440747	28.405131266755788	28.973479480995636	22.926649067841108
75-79	19.81828476187638	28.64652769143347	29.205648339294942	22.329539207395204
80-84	21.503620638099573	28.98705366271703	27.018180771930112	22.491144927253277
85-89	20.6971744945133	27.979820607067	27.223006028745207	24.099998869674497
90-94	20.8512275848746	29.835249920106538	28.608795371008792	20.704727124010066
95-99	22.153372839922277	28.310944325303574	28.443428749931925	21.09225408484223
100-104	21.175713209703126	27.937495054825924	28.26727265814538	22.619519077325567
105-109	22.766862144474096	28.486545530025044	26.715664564603752	22.03092776089711
110-114	21.024855857809165	27.92804142334698	29.75856247257676	21.2885402462671
115-119	22.057377377665386	28.40371322203395	28.412642793506997	21.126266606793667
120-124	20.101975911735963	28.090037619287873	27.72681265676844	24.08117381220772
125-129	20.234460336364318	26.586982143939757	30.51361990852584	22.66493761117008
130-134	22.85409244624018	28.46793626197246	28.534019201147586	20.143952090639775
135-139	22.67362056616977	26.737335987200606	27.8399685152969	22.749074931332725
140-144	20.761705805043512	28.06278649898117	28.356424513266425	22.819083182708898
145-149	21.31963447328373	27.857365252355443	29.381300922448368	21.44169935191246
150-154	21.993464868298055	27.538883458188074	27.034162242212545	23.43348943130133
155-159	21.012977164342136	26.84411064448077	31.09418591388358	21.048726277293508
160-164	23.165520054082997	29.785035701334678	26.668388871882627	20.3810553726997
165-169	21.880625296068214	27.71330012916538	28.44455907543484	21.961515499331565
170-174	21.401531693813318	30.374086363033836	27.610163064867326	20.614218878285524
175-179	20.103620021558392	28.826290549142957	28.76894194339946	22.30114748589919
180-184	21.818447117721345	28.835497564148543	27.631392632949396	21.714662685180713
185-189	21.71512509106827	28.140963921037514	28.591758282973906	21.552152704920307
190-194	21.301045756603926	27.395257359703486	28.04228650463279	23.261410379059797
195-199	21.76708923714332	30.242660334103665	27.48686510387178	20.503385324881236
200-204	23.163996872081064	27.27240125322271	27.429670633424134	22.13393124127209
205-209	22.62666067936673	27.12354766017483	29.04525515043091	21.204536510027527
210-214	24.854285629144314	26.68048061440384	29.144621037823775	19.320612718628073
215-219	23.705504993469802	27.832662502273497	28.428981134867353	20.032851369389345
220-224	22.565766961818632	27.408266378673428	28.30087415264121	21.725092506866726
225-229	24.073056019959495	27.989644163255996	27.555157315620786	20.38214250116372
230-234	23.093382355207872	27.978423113717938	27.404341066472387	21.523853464601803
235-239	24.054734471125837	26.584906455288948	26.864559260397197	22.49579981318802
240-244	24.29914167191583	29.827789771787284	25.868259535066297	20.004809021230596
245-249	24.96096780524697	28.057042390289066	26.130813598021312	20.851176206442652
250-254	23.88075927876594	27.247269724156364	26.011803271141794	22.860167725935906
255-259	24.422090259574112	27.42916712479102	26.431274668069644	21.71746794756523
260-264	22.555409069937348	28.765283799044568	27.433010231500937	21.246296899517144
265-269	24.024678088434612	28.110599267754587	26.711410430438224	21.153312213372573
270-274	22.306377810271783	29.63954947280591	26.001956490688688	22.052116226233622
275-279	23.331408354749577	28.850839163929052	25.208509090385967	22.609243390935408
280-284	21.7023524128853	29.010739119846356	26.06634394160944	23.2205645256589
285-289	23.78428868102317	28.522582362195337	25.565198716361255	22.127930240420234
290-294	22.54702410984298	29.827800047473673	25.556341074692934	22.068834767990413
295-299	22.35986275793257	29.628575039741218	24.57941101820749	23.432151184118723
300	13.648636081755457	40.19557723954846	22.990164621718392	23.165622056977682
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.25
6	0.25
7	0.25
8	0.5
9	0.75
10	0.75
11	0.5
12	1.0
13	1.0
14	1.5
15	2.75
16	3.0
17	2.75
18	5.25
19	7.25
20	12.0
21	11.5
22	17.5
23	27.75
24	41.5
25	53.5
26	65.5
27	83.25
28	117.0
29	130.25
30	158.25
31	192.0
32	335.75
33	770.75
34	1848.0
35	3565.5
36	7617.75
37	15895.0
38	24752.25
39	30955.0
40	40473.5
41	45244.5
42	52564.75
43	63204.25
44	66691.75
45	69163.0
46	60484.5
47	46333.5
48	37615.0
49	30646.5
50	23759.5
51	15706.75
52	6886.75
53	2200.75
54	600.0
55	198.75
56	89.75
57	60.0
58	40.5
59	28.5
60	21.0
61	24.75
62	19.25
63	16.25
64	8.25
65	8.5
66	6.75
67	6.75
68	6.75
69	4.75
70	6.0
71	6.5
72	5.25
73	6.0
74	5.75
75	4.25
76	4.75
77	3.5
78	2.75
79	2.75
80	2.5
81	3.0
82	3.0
83	4.5
84	2.0
85	1.25
86	3.25
87	3.5
88	1.25
89	1.0
90	1.0
91	2.25
92	2.0
93	2.0
94	1.5
95	2.0
96	1.75
97	1.75
98	0.5
99	1.25
100	1.3333333333333333
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.0275686390161647E-5
155-159	0.0
160-164	1.0275686390161647E-5
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	1.0275686390161647E-5
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300	5.137843195080824E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	1946342.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.955434388068024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.5279455628102	9.982035512150992
2	2.280206345291184	0.63642540085935
3	1.8728363688649041	0.7840873519584521
4	1.3921850436487728	0.7771418813076028
5	1.5241940608647158	1.063539510554025
6	1.5152811690030819	1.2687844160098514
7	1.5667056416904763	1.5304840451619217
8	1.4161012663223325	1.580984664721708
9	0.9300920048501484	1.1681854153697604
>10	14.318641963115859	41.902916873712705
>50	1.3699221000546262	12.715776022932006
>100	0.2526290576859716	4.8905366526931555
>500	0.00560284850190819	0.5545834292107842
>1k	0.01819704676950233	6.684818381851276
>5k	0.005457415688302904	5.1659231696695365
>10k+	0.0040021048380884956	9.293777271836838
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	35250	1.8110897262659902	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	27333	1.4043266805114414	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	17848	0.9170022534580253	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT	16785	0.8623869802943163	Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	16287	0.8368005211828138	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	15540	0.79842083251556	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	12193	0.6264572207762048	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA	10836	0.556736688618958	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTTAATTGTCGTACTCTTC	10548	0.5419397002171252	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC	10244	0.5263206569040796	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	10179	0.5229810588272771	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC	9137	0.46944473273453485	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG	9087	0.4668758111369944	No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA	8483	0.4358432382387063	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	8399	0.4315274499548384	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	7720	0.3966414946602396	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	6863	0.3526101784783969	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG	6774	0.34803749803477496	No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA	6439	0.3308257233312542	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	6349	0.3262016644556815	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	6104	0.31361394862773345	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	5796	0.29778939158688456	No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	5233	0.2688633343985795	No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	5149	0.2645475461147116	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	5121	0.26310895002008894	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	5090	0.2615162186296139	No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA	4936	0.25360394010918946	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4934	0.2535011832452878	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA	4893	0.2513946675353047	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA	4865	0.24995607144068208	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAACATTAATTG	4740	0.24353376744683103	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT	4652	0.2390124654351599	No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	4612	0.23695732815712756	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT	4509	0.23166534966619434	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG	4263	0.2190262554062955	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	3847	0.19765282771475925	No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	3830	0.19677939437159556	No Hit
CCGGGTTATTAGTAGTAACAAGAGCACCGATTATCACCAGAAGAGGGAGA	3251	0.16703128227207756	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT	3246	0.16677439011232353	No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAGTCATTTGTGT	3233	0.16610647049696303	No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	3203	0.16456511753843878	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT	3069	0.15768040765703048	No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA	3054	0.15690973117776835	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	2965	0.1523370507341464	No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	2941	0.151103968367327	No Hit
GGGGGGAGCGAAAGCAGGCCTACCAGAAGCGAATGGGAGTGCAGATGCAG	2798	0.14375685259836146	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2675	0.137437305468412	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	2583	0.13271048972893768	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTTTAT	2558	0.13142602893016747	No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT	2516	0.1292681347882335	No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGT	2496	0.12824056614921733	No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	2387	0.12264031706657925	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG	2381	0.1223320464748744	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	2379	0.12222928961097279	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT	2372	0.12186964058731713	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACA	2359	0.12120172097195663	No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT	2202	0.11313530715567972	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	5.651627514588906E-4	0.0	0.0
2	5.651627514588906E-4	0.0	0.0
3	6.67919615360507E-4	0.0	5.137843195080823E-5
4	8.220549112129317E-4	0.0	5.137843195080823E-5
5	9.761902070653564E-4	0.0	5.137843195080823E-5
6	0.0011303255029177812	0.0	5.137843195080823E-5
7	0.0015413529585242471	0.0	5.137843195080823E-5
8	0.0015413529585242471	0.0	5.137843195080823E-5
9	0.0017982451182782882	0.0	5.137843195080823E-5
10-14	0.0023839592425175023	0.0	5.137843195080823E-5
15-19	0.0037197984732385164	0.0	5.137843195080823E-5
20-24	0.0052714271181529245	0.0	5.137843195080823E-5
25-29	0.006504509484972322	0.0	5.137843195080823E-5
30-34	0.009628318147581463	0.0	5.137843195080823E-5
35-39	0.010851124828010698	0.0	5.137843195080823E-5
40-44	0.012197239745121874	0.0	5.137843195080823E-5
45-49	0.014314031141495173	0.0	5.137843195080823E-5
50-54	0.017078190780448657	0.0	5.137843195080823E-5
55-59	0.021095984159001862	0.0	5.137843195080823E-5
60-64	0.026418789709105595	0.0	5.137843195080823E-5
65-69	0.03465989019401523	0.0	5.137843195080823E-5
70-74	0.04474033854276381	0.0	5.137843195080823E-5
75-79	0.057410259861833124	0.0	5.137843195080823E-5
80-84	0.07548519222212746	0.0	5.137843195080823E-5
85-89	0.10117440819753158	0.0	5.137843195080823E-5
90-94	0.13739620272285138	0.0	5.137843195080823E-5
95-99	0.18386285657916238	0.0	5.137843195080823E-5
100-104	0.24462299020418818	0.0	5.137843195080823E-5
105-109	0.3222044224499086	0.0	5.137843195080823E-5
110-114	0.42466329144621034	0.0	5.137843195080823E-5
115-119	0.5511878179682708	0.0	5.137843195080823E-5
120-124	0.7059499306904952	0.0	5.137843195080823E-5
125-129	0.9017223077958549	0.0	5.137843195080823E-5
130-134	1.1383713653612777	0.0	5.137843195080823E-5
135-139	1.428967776475049	0.0	5.137843195080823E-5
140-144	1.7916995060477554	0.0	5.137843195080823E-5
145-149	2.212920442553261	0.0	5.137843195080823E-5
150-154	2.703789981411284	0.0	5.137843195080823E-5
155-159	3.2985878124194	0.0	5.137843195080823E-5
160-164	3.94953199386336	0.0	5.137843195080823E-5
165-169	4.634951103146312	0.0	5.137843195080823E-5
170-174	5.4528546370576185	0.0	5.137843195080823E-5
175-179	6.391692724094737	0.0	5.137843195080823E-5
180-184	7.423104469820823	0.0	5.137843195080823E-5
185-189	8.53857133021843	0.0	5.137843195080823E-5
190-194	9.759929138866655	0.0	5.137843195080823E-5
195-199	11.062053842541546	0.0	5.137843195080823E-5
200-204	12.411570011847868	4.1102745560646586E-5	7.192980473113151E-5
205-209	13.83435182511604	5.137843195080823E-5	1.0275686390161647E-4
210-214	15.270214587158886	5.137843195080823E-5	1.0275686390161647E-4
215-219	16.706323965675097	5.137843195080823E-5	1.0275686390161647E-4
220-224	18.144796752061048	5.137843195080823E-5	1.4385960946226305E-4
225-229	19.613346472511	5.137843195080823E-5	1.541352958524247E-4
230-234	21.044328283518517	5.137843195080823E-5	1.541352958524247E-4
235-239	22.416142692291487	5.137843195080823E-5	1.541352958524247E-4
240-244	23.723210001120048	5.137843195080823E-5	1.541352958524247E-4
245-249	24.936840493602876	5.137843195080823E-5	1.541352958524247E-4
250-254	26.083730402981598	5.137843195080823E-5	1.541352958524247E-4
255-259	27.22199901147897	5.137843195080823E-5	2.0551372780323293E-4
260-264	28.28129897006795	5.137843195080823E-5	2.0551372780323293E-4
265-269	29.257478901446923	5.137843195080823E-5	2.466164733638795E-4
270-274	30.087600226476127	5.137843195080823E-5	2.5689215975404115E-4
275-279	30.886894492334854	5.137843195080823E-5	2.5689215975404115E-4
280-284	31.57227249887224	5.137843195080823E-5	2.671678461442028E-4
285-288	32.15195479520043	5.137843195080823E-5	3.2111519969255147E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGGGGA	23045	0.0	283.85767	1
CCGGGTT	7800	0.0	279.1115	1
GGAGCGA	12155	0.0	264.49115	5
CGAAAGC	12795	0.0	251.83588	9
GGGGAGC	26140	0.0	251.31714	3
GCGAAAG	12950	0.0	248.25406	8
GGGAGCG	13120	0.0	245.93369	4
GAGCGAA	13150	0.0	244.81367	6
GGGGGAG	27070	0.0	242.90025	2
AGCGAAA	13250	0.0	242.63321	7
TAGTAGA	19610	0.0	228.5584	9
GGAGCAG	8460	0.0	212.8546	5
CGGGTTA	22970	0.0	196.21332	1
GGGTTAT	23965	0.0	188.31212	2
GGGAGCA	17880	0.0	187.53188	4
GTTATTA	24930	0.0	180.61012	4
TATTAGT	24935	0.0	180.16121	6
ATTAGTA	25075	0.0	179.44846	7
TTATTAG	25230	0.0	178.11296	5
GGTTATT	25495	0.0	177.06883	3
>>END_MODULE