FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n02 clinical flu pool e4.3420000000b77d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n02 clinical flu pool e4.3420000000b77d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946342
Sequences flagged as poor quality0
Sequence length300
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA352501.8110897262659902No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC273331.4043266805114414No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC178480.9170022534580253No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT167850.8623869802943163Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG162870.8368005211828138No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG155400.79842083251556No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG121930.6264572207762048No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA108360.556736688618958No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTTAATTGTCGTACTCTTC105480.5419397002171252No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC102440.5263206569040796No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG101790.5229810588272771No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC91370.46944473273453485No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG90870.4668758111369944No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA84830.4358432382387063No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG83990.4315274499548384No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG77200.3966414946602396No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA68630.3526101784783969No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG67740.34803749803477496No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA64390.3308257233312542No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA63490.3262016644556815No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT61040.31361394862773345No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG57960.29778939158688456No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT52330.2688633343985795No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC51490.2645475461147116No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT51210.26310895002008894No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG50900.2615162186296139No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA49360.25360394010918946No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA49340.2535011832452878No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA48930.2513946675353047No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA48650.24995607144068208No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAACATTAATTG47400.24353376744683103No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT46520.2390124654351599No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA46120.23695732815712756No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT45090.23166534966619434No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG42630.2190262554062955No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG38470.19765282771475925No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA38300.19677939437159556No Hit
CCGGGTTATTAGTAGTAACAAGAGCACCGATTATCACCAGAAGAGGGAGA32510.16703128227207756No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT32460.16677439011232353No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAGTCATTTGTGT32330.16610647049696303No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT32030.16456511753843878No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT30690.15768040765703048No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA30540.15690973117776835No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT29650.1523370507341464No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC29410.151103968367327No Hit
GGGGGGAGCGAAAGCAGGCCTACCAGAAGCGAATGGGAGTGCAGATGCAG27980.14375685259836146No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT26750.137437305468412No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA25830.13271048972893768No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTTTAT25580.13142602893016747No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT25160.1292681347882335No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGT24960.12824056614921733No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA23870.12264031706657925No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG23810.1223320464748744No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA23790.12222928961097279No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT23720.12186964058731713No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACA23590.12120172097195663No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT22020.11313530715567972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA230450.0283.857671
CCGGGTT78000.0279.11151
GGAGCGA121550.0264.491155
CGAAAGC127950.0251.835889
GGGGAGC261400.0251.317143
GCGAAAG129500.0248.254068
GGGAGCG131200.0245.933694
GAGCGAA131500.0244.813676
GGGGGAG270700.0242.900252
AGCGAAA132500.0242.633217
TAGTAGA196100.0228.55849
GGAGCAG84600.0212.85465
CGGGTTA229700.0196.213321
GGGTTAT239650.0188.312122
GGGAGCA178800.0187.531884
GTTATTA249300.0180.610124
TATTAGT249350.0180.161216
ATTAGTA250750.0179.448467
TTATTAG252300.0178.112965
GGTTATT254950.0177.068833
Produced by FastQC (version 0.11.2)