FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n02 clinical flu pool e3.3420000000b703.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n02 clinical flu pool e3.3420000000b703.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568177
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT452837.9698755845449565Illumina Single End PCR Primer 1 (96% over 33bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC234104.120194939253085No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG120292.117121953194163No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA95231.6760622130075662No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA76381.3442993996589092No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATACTCCTC69581.2246183847639027No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG67741.192234110145254No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG64021.126761554937986No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA55730.9808563176615737No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG34210.6021011058173774No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC34190.601749102832392No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA31870.5609167565740958No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG27000.4752040297301721No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTCAAATT25890.4556678640634872No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG21770.3831552491565129No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC20470.3602750551324675No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT19240.3386268715558708No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGCCCTGGTGTAGATCT17740.31222664768197234Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA13630.23989003426749061No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA13220.2326739730752917No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGACCTGGTGTAGATCT12690.22334589397318091Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA12650.22264188800321028No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA9530.16772942234550148No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA9270.16315338354069242No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGGCCTGGTGTAGATCT6350.11176094773283676Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA60650.0292.788151
GGGTTAT61300.0289.683532
GGGGGGA55800.0285.56991
GGTTATT63200.0280.50953
ATTAGTA63500.0279.184277
TTATTAG64100.0275.653665
GGGGGAG58550.0274.66782
TATTAGT65150.0271.887946
GTTATTA66400.0266.32684
GGAGCGA39950.0260.147675
GAGCGAA40700.0254.992636
TTAGTAG69900.0254.042918
GCGAAAG40850.0253.696448
CGAAAGC40950.0253.435919
GGGGAGC66250.0242.744143
TAGTAGA73500.0241.49
AGCGAAA45500.0227.12317
GGGAGCG46100.0225.44254
GAGCAAA28400.0197.207756
GCAAAAG31000.0179.719358
Produced by FastQC (version 0.11.2)