FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n02 clinical flu pool d4.3420000000b760.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n02 clinical flu pool d4.3420000000b760.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854480
Sequences flagged as poor quality0
Sequence length300
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT751098.790024342290048Illumina Single End PCR Primer 1 (96% over 32bp)
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA97741.1438535717629434No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC87791.0274084823518397No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG80340.9402209530942794No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA74240.868832506319633No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA58360.6829884842243236No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT48230.5644368504821645No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT47820.5596386106169834No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA45950.5377539556221328No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA44270.5180928751989514No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG40110.4694082951034548No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT30440.3562400524295478No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAGTCATTTGTGT29560.34594139125550044No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT29010.33950472802172077No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA28800.3370470929688231No Hit
CCGGGTTATTAGTAGTAACAAGAGCACCGATTATCACCAGAAGAGGGAGA28640.3351746091189964No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT26990.31586461941765753No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT26810.3137580750866024No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTTTAT25540.2988952345286022No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATAAGA24700.28906469431701154No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG24660.2885965733545548No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGT23170.27115906750304275No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT23080.2701057953375152No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTG20020.23429454170957775No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCCAAAATTGAA18850.2206020035577193No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC15490.1812798427113566No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCGCCTGTGTAGATCT14940.17484317947757702Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCCAAAATTGAA14790.17308772586836438No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTTTAT14080.164778578784758No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA12530.1466388914895609No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAAGGCCTGATTCTG11420.1336485347813875No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT9540.11164684954592266No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC9360.10954030521486752No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGT9140.10696563992135567No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT72350.0291.155461
CGGGTTA73000.0288.563022
GGGGGGA61150.0281.01881
GTTATTA76450.0275.34865
GGGTTAT81450.0258.445683
TTAGTAG82550.0254.467599
TATTAGT83500.0251.74857
ATTAGTA83850.0250.522378
GGGGAGC69550.0247.923813
TTATTAG85500.0245.859656
CAGAAGC70100.0244.720419
GGAGCAG71050.0241.862095
GAGCAGA73200.0234.75826
GGTTATT92600.0227.802374
GGGAGCA76400.0224.925384
GGGGGAG78300.0220.781622
GCAGAAG91650.0187.980368
AGCAGAA103450.0166.538427
CGGGTGA754.521222E-578.41
ATAGGCC14250.055.4989540-44
Produced by FastQC (version 0.11.2)