##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE77A l01n02 clinical flu pool d3.3420000000b6f5.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	843876
Sequences flagged as poor quality	0
Sequence length	300
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.98795676141992	34.0	34.0	34.0	33.0	34.0
2	33.26983348264437	34.0	34.0	34.0	34.0	34.0
3	33.310538515137296	34.0	34.0	34.0	34.0	34.0
4	33.256229588233346	34.0	34.0	34.0	34.0	34.0
5	33.267927989420244	34.0	34.0	34.0	34.0	34.0
6	36.66533827244761	38.0	38.0	38.0	35.0	38.0
7	36.844941673895214	38.0	38.0	38.0	36.0	38.0
8	36.80868634728325	38.0	38.0	38.0	36.0	38.0
9	36.72246751892458	38.0	38.0	38.0	35.0	38.0
10-14	36.78411140973319	38.0	38.0	38.0	35.6	38.0
15-19	36.817850489882396	38.0	38.0	38.0	36.0	38.0
20-24	36.77011906962635	38.0	38.0	38.0	35.6	38.0
25-29	36.66888215804217	38.0	38.0	38.0	35.0	38.0
30-34	36.765415534983816	38.0	38.0	38.0	35.8	38.0
35-39	36.69548464466344	38.0	38.0	38.0	35.2	38.0
40-44	36.58456431987638	38.0	38.0	38.0	34.6	38.0
45-49	36.59630822537908	38.0	38.0	38.0	34.4	38.0
50-54	36.491291374562145	38.0	38.0	38.0	34.0	38.0
55-59	36.51499556806924	38.0	38.0	38.0	34.0	38.0
60-64	36.46327161810503	38.0	37.2	38.0	34.0	38.0
65-69	36.188796221245774	38.0	37.0	38.0	33.6	38.0
70-74	36.36609667771094	38.0	37.0	38.0	34.0	38.0
75-79	36.25649029004261	38.0	37.0	38.0	33.4	38.0
80-84	36.0020751864018	38.0	37.0	38.0	32.8	38.0
85-89	36.08292616450758	38.0	37.0	38.0	32.8	38.0
90-94	36.006101133341865	38.0	37.0	38.0	32.6	38.0
95-99	35.84405196972067	38.0	37.0	38.0	31.8	38.0
100-104	35.88839545146443	38.0	37.0	38.0	31.8	38.0
105-109	35.640258995397424	38.0	37.0	38.0	30.6	38.0
110-114	35.47924529196232	38.0	37.0	38.0	29.4	38.0
115-119	35.527318231588524	38.0	37.0	38.0	30.0	38.0
120-124	35.28849428115031	38.0	37.0	38.0	28.2	38.0
125-129	35.24355071124194	38.0	36.8	38.0	27.8	38.0
130-134	35.19659950040053	38.0	36.2	38.0	27.6	38.0
135-139	34.85025145874512	38.0	36.0	38.0	25.2	38.0
140-144	34.72593248297144	38.0	35.8	38.0	24.2	38.0
145-149	34.47490271082482	38.0	34.8	38.0	24.0	38.0
150-154	34.26229706734165	38.0	34.6	38.0	23.6	38.0
155-159	34.03457593295697	37.4	34.0	38.0	23.2	38.0
160-164	33.79244699458214	37.0	34.0	38.0	23.0	38.0
165-169	33.047523332811934	37.0	32.4	38.0	20.0	38.0
170-174	32.38868885950069	36.6	29.4	38.0	18.8	38.0
175-179	32.74366636804459	37.0	31.0	38.0	19.8	38.0
180-184	32.78782901753338	37.0	31.0	38.0	20.8	38.0
185-189	32.50438500443194	37.0	30.4	38.0	20.0	38.0
190-194	31.821758173001722	37.0	28.6	37.8	17.4	38.0
195-199	31.387877602870567	35.4	28.0	37.4	16.2	38.0
200-204	30.800978342789698	35.0	26.6	37.0	14.8	37.8
205-209	29.957512952139886	34.4	24.4	37.0	10.8	37.6
210-214	29.28866255231811	33.8	23.4	37.0	10.0	37.0
215-219	28.368167361081486	32.2	21.8	37.0	10.0	37.0
220-224	27.280909280510407	30.6	20.4	36.6	9.4	37.0
225-229	26.334356706435546	29.2	19.4	35.8	9.0	37.0
230-234	25.40011091676977	27.8	18.0	34.4	9.0	37.0
235-239	24.283930340476562	25.8	15.8	33.6	8.6	37.0
240-244	23.497891633367935	24.6	14.6	32.8	8.0	36.8
245-249	22.385994624802695	23.0	13.2	31.4	8.0	36.2
250-254	21.29170826045533	21.2	11.2	30.2	8.0	36.0
255-259	20.216741085183134	19.6	9.4	28.8	7.8	34.8
260-264	19.618379951556864	19.0	9.0	27.8	7.4	34.2
265-269	18.9054330257052	18.4	8.8	26.6	7.2	33.4
270-274	17.900846569875192	17.0	8.0	25.0	7.0	32.2
275-279	16.919632268247945	15.2	8.0	23.4	7.0	30.6
280-284	16.028818926003346	13.4	8.0	21.8	7.0	29.4
285-289	15.592240803151174	13.0	8.0	21.2	7.0	28.8
290-294	14.923023761784908	13.0	8.0	20.4	7.0	27.2
295-299	14.323126620498746	12.0	8.0	19.0	7.0	26.4
300	12.308047627850538	11.0	8.0	14.0	7.0	21.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.053117844954229554
1101	2	0.061863936161891786
1101	3	0.05610084316301567
1101	4	-0.016411506500404016
1101	5	-0.008760938057186252
1101	6	0.09674227041963235
1101	7	-0.03388430724592695
1101	8	-0.030348523963695584
1101	9	0.03755044969293664
1101	10-14	0.10552174998542796
1101	15-19	0.18054761692575738
1101	20-24	0.06396706262042073
1101	25-29	0.053822468798031764
1101	30-34	0.10667614185216223
1101	35-39	0.0838361762423574
1101	40-44	0.12321536663058907
1101	45-49	0.0718194313580085
1101	50-54	0.1139658506747736
1101	55-59	0.031152750874376522
1101	60-64	0.18582478609632602
1101	65-69	0.22402151029051254
1101	70-74	0.12698708367859268
1101	75-79	0.14115424761692452
1101	80-84	0.21809767439786043
1101	85-89	0.140460990020145
1101	90-94	0.10798117799787121
1101	95-99	0.06206384501775375
1101	100-104	0.16345666587705665
1101	105-109	0.15193814506996262
1101	110-114	0.30763228167582213
1101	115-119	0.16804913149400136
1101	120-124	0.13774790725504715
1101	125-129	0.19186430531396326
1101	130-134	-0.0017708365940833914
1101	135-139	0.088352857029804
1101	140-144	0.033303314154828456
1101	145-149	0.3127546201087412
1101	150-154	0.12966439222038417
1101	155-159	0.12635368663092805
1101	160-164	0.28629578662179966
1101	165-169	0.4391518240148642
1101	170-174	1.217989780264844
1101	175-179	0.16496912778592332
1101	180-184	-0.18280367113723628
1101	185-189	-0.2890574875847136
1101	190-194	-0.8692982212679183
1101	195-199	-0.9166848842817714
1101	200-204	-0.5028119255561059
1101	205-209	-0.33925597937747654
1101	210-214	-0.9196834016188014
1101	215-219	-0.7961108164440134
1101	220-224	-1.0943207165159947
1101	225-229	-0.8572623607125429
1101	230-234	-0.6487769744909464
1101	235-239	-0.7282778157435317
1101	240-244	-0.8719950703660366
1101	245-249	-0.8326087530603061
1101	250-254	-0.49682414074683834
1101	255-259	-0.3943473196093983
1101	260-264	-0.42387770340745234
1101	265-269	-0.757487907860618
1101	270-274	-0.6594630449278185
1101	275-279	-0.46371152200125465
1101	280-284	-0.24227674856173387
1101	285-289	-0.4015018857336923
1101	290-294	-0.44883939372166637
1101	295-299	-0.3449922074808214
1101	300	0.5712297695017146
1102	1	-0.10019374515419344
1102	2	0.025170456030004118
1102	3	-0.1191923844105105
1102	4	-0.07890658924147687
1102	5	-0.026257638030088515
1102	6	0.1993409275211917
1102	7	0.0468630445866296
1102	8	0.01699325553170894
1102	9	-0.056810854995916316
1102	10-14	-0.04130907167164821
1102	15-19	-0.05777513486373209
1102	20-24	-0.07784914832065226
1102	25-29	-0.1075499292269555
1102	30-34	0.015203920490876044
1102	35-39	-0.04930348662639972
1102	40-44	0.03047693619579661
1102	45-49	-0.11163354534193104
1102	50-54	-0.12436333825366575
1102	55-59	-0.05215928427081451
1102	60-64	0.029660351368519855
1102	65-69	0.08266344880709653
1102	70-74	-0.0123604447381922
1102	75-79	0.005952157215659781
1102	80-84	-0.03128221454957725
1102	85-89	-0.0738036701664555
1102	90-94	-0.11398750571847671
1102	95-99	-0.04831548524958862
1102	100-104	-0.0739948072343779
1102	105-109	0.05480493682375709
1102	110-114	0.0663849230882434
1102	115-119	0.17326877700632792
1102	120-124	-0.041841911893136796
1102	125-129	0.03115890614379424
1102	130-134	0.04912742243797652
1102	135-139	0.0988127310049336
1102	140-144	0.02263534575561721
1102	145-149	-0.04026137816832431
1102	150-154	-0.1312851016694978
1102	155-159	0.03903453248686617
1102	160-164	9.701190467836796E-4
1102	165-169	0.3437553717526072
1102	170-174	1.1643573663297886
1102	175-179	0.6545088564565447
1102	180-184	0.4132741642039264
1102	185-189	0.31255370445421704
1102	190-194	-0.06829049113821384
1102	195-199	-0.054411286803222936
1102	200-204	0.49323392276741984
1102	205-209	0.051468143125255494
1102	210-214	0.3327427242646124
1102	215-219	0.15453579813745932
1102	220-224	0.15881818343259013
1102	225-229	0.31107196203288723
1102	230-234	-0.14062707975008948
1102	235-239	0.14723646000283708
1102	240-244	0.2309314437324943
1102	245-249	0.433729540048855
1102	250-254	0.9005089764257797
1102	255-259	0.8664312324793677
1102	260-264	0.7730039398590236
1102	265-269	0.5917506421778072
1102	270-274	0.556579378202013
1102	275-279	0.9618470190092836
1102	280-284	1.0307330033064268
1102	285-289	0.8120664905894
1102	290-294	0.709873234117131
1102	295-299	0.4535146544298314
1102	300	1.139930731019545
1103	1	-0.1832299241402282
1103	2	-0.03949289672399914
1103	3	0.024194756565833586
1103	4	-0.02055218855342389
1103	5	0.07467888190354444
1103	6	0.13893961711988112
1103	7	-0.04063716273035567
1103	8	-0.012940750535371137
1103	9	0.09140302031535441
1103	10-14	0.011132253672279546
1103	15-19	-0.12035959185513434
1103	20-24	-0.14662136579104157
1103	25-29	-0.06982629343196578
1103	30-34	-0.05747596549662859
1103	35-39	-0.0770480278714274
1103	40-44	-0.059181855584498066
1103	45-49	-0.11132576846713249
1103	50-54	-0.17356175206152358
1103	55-59	-0.0701015229941504
1103	60-64	-0.31043657030988925
1103	65-69	-0.04443434241798627
1103	70-74	-0.17152507969839093
1103	75-79	-0.08778189418474369
1103	80-84	0.21946294193328697
1103	85-89	0.1739840009095417
1103	90-94	0.10817250824481306
1103	95-99	0.025875524380182924
1103	100-104	-6.441224564923687E-4
1103	105-109	0.08754431686303832
1103	110-114	0.13960967760518628
1103	115-119	0.07535102932965998
1103	120-124	0.0401875358065098
1103	125-129	0.09415902382085761
1103	130-134	0.1021811819044629
1103	135-139	0.042897562746468054
1103	140-144	0.15153774249793628
1103	145-149	0.26962187670046944
1103	150-154	0.30795889818201516
1103	155-159	0.2062745787047362
1103	160-164	0.41975118204192796
1103	165-169	0.7717282775943701
1103	170-174	1.4941457766389377
1103	175-179	0.9584915539783054
1103	180-184	0.6101875894201498
1103	185-189	0.6662890826833276
1103	190-194	0.5916051237718669
1103	195-199	0.6231411407009837
1103	200-204	0.6073238169923201
1103	205-209	0.8565743407579696
1103	210-214	0.8598554423127212
1103	215-219	0.8952999251118108
1103	220-224	0.8217677552539051
1103	225-229	1.0518165419770789
1103	230-234	0.990317882921012
1103	235-239	0.7748486737770577
1103	240-244	0.9324087253447786
1103	245-249	1.0622445107046161
1103	250-254	1.3180705021840282
1103	255-259	1.4498790837671471
1103	260-264	0.9755169376468515
1103	265-269	0.6945627292714782
1103	270-274	0.9304300720623466
1103	275-279	1.2544750639034774
1103	280-284	1.3112342681374898
1103	285-289	0.7193949288725126
1103	290-294	0.7123107685218795
1103	295-299	0.6281099519277333
1103	300	1.4548404948203402
2116	1	0.04605990999139209
2116	2	0.06069273129236308
2116	3	-0.04832401429452915
2116	4	0.09480171339978938
2116	5	0.12801820468624925
2116	6	0.16310197267758042
2116	7	0.13410343757257692
2116	8	0.05165296426802257
2116	9	-0.016015125417702336
2116	10-14	0.08281090320072337
2116	15-19	0.03295091974741382
2116	20-24	-0.04325608520501589
2116	25-29	0.020744484977164745
2116	30-34	0.04050693484997936
2116	35-39	-0.014841004284299686
2116	40-44	0.045189993316519406
2116	45-49	0.019797677334160824
2116	50-54	-0.0339434070676532
2116	55-59	0.03055590295873145
2116	60-64	-0.11434026725591195
2116	65-69	0.19282850035381216
2116	70-74	0.10394190316469576
2116	75-79	0.04882617785985133
2116	80-84	0.2143674845885002
2116	85-89	0.20516102678728743
2116	90-94	0.1568559918692074
2116	95-99	0.23810969467763954
2116	100-104	0.23120610220730242
2116	105-109	0.3424428119445295
2116	110-114	0.2871105950451138
2116	115-119	0.22207856275181115
2116	120-124	0.3469593916824536
2116	125-129	0.3902365817741327
2116	130-134	0.2911138445885584
2116	135-139	0.3190757101598365
2116	140-144	0.4286398291882918
2116	145-149	0.47991709816610495
2116	150-154	0.45184446825897595
2116	155-159	0.5456780485695134
2116	160-164	0.5332973904985394
2116	165-169	0.9849870083330785
2116	170-174	1.8342053250286412
2116	175-179	1.1533009444415256
2116	180-184	0.8235511421279611
2116	185-189	0.8071372331006685
2116	190-194	0.8789891557994345
2116	195-199	0.6102410239503158
2116	200-204	0.8738033761138908
2116	205-209	0.9720613210371702
2116	210-214	0.885902651432751
2116	215-219	1.3107062578646875
2116	220-224	1.538458424632335
2116	225-229	0.7642959837931613
2116	230-234	1.2528247488590587
2116	235-239	0.9296031129577251
2116	240-244	0.9832957688738375
2116	245-249	0.9355299284429464
2116	250-254	1.4152024225145787
2116	255-259	1.6886090774342328
2116	260-264	1.4852381944325401
2116	265-269	1.1869489687030814
2116	270-274	1.2667469807772775
2116	275-279	1.3774899260338564
2116	280-284	1.3963470518206087
2116	285-289	1.1871689303293227
2116	290-294	0.9073064210852042
2116	295-299	0.6095937554473778
2116	300	0.7813573618442593
1117	1	0.1033153356052594
1117	2	-0.07234462570635003
1117	3	0.04907681586099244
1117	4	-0.04007468637357192
1117	5	-0.05027026799310619
1117	6	0.015851226237892035
1117	7	0.0728707139112359
1117	8	0.0453519946922043
1117	9	-0.06064075965270632
1117	10-14	0.04562770239093794
1117	15-19	-0.06214867981713468
1117	20-24	-0.014581819389732686
1117	25-29	0.06423247509678731
1117	30-34	0.1514633644172676
1117	35-39	0.03462486957534594
1117	40-44	0.07502800476298432
1117	45-49	0.1284286846616851
1117	50-54	0.16136198066717355
1117	55-59	0.14060068934011127
1117	60-64	0.1702709793142887
1117	65-69	0.3316560966588611
1117	70-74	0.22454968650939122
1117	75-79	0.23262779997356375
1117	80-84	0.1938795453986657
1117	85-89	0.14631086222041745
1117	90-94	0.1277816089154058
1117	95-99	0.2703129172599006
1117	100-104	0.3488634593333373
1117	105-109	0.3722074526667143
1117	110-114	0.35113968231966197
1117	115-119	0.2768411344514021
1117	120-124	0.3722684718268354
1117	125-129	0.3031122685304837
1117	130-134	0.34070179078123175
1117	135-139	0.3583527618344746
1117	140-144	0.4681366747246116
1117	145-149	0.6450324223023642
1117	150-154	0.5862243026098497
1117	155-159	0.7890223211966685
1117	160-164	0.8429888514831418
1117	165-169	1.1885520934176554
1117	170-174	1.5822443690765482
1117	175-179	1.0736149265778963
1117	180-184	0.6641684429765746
1117	185-189	0.826813319655848
1117	190-194	1.0336852004648627
1117	195-199	0.9534707045568922
1117	200-204	0.9238937764856736
1117	205-209	0.7604436000178119
1117	210-214	0.49447190639363114
1117	215-219	1.0108531584688407
1117	220-224	1.525438383761177
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2108	220-224	-0.534554631541944
2108	225-229	-0.6447046421660296
2108	230-234	-0.5375890197012652
2108	235-239	-0.456125811758767
2108	240-244	-0.8159098666653968
2108	245-249	0.14197746702523162
2108	250-254	-0.4313092053923988
2108	255-259	0.5047129104016328
2108	260-264	-0.1752890411473622
2108	265-269	-0.34859725218743165
2108	270-274	-0.22909325028746252
2108	275-279	-0.06292098281441127
2108	280-284	0.24560289119376222
2108	285-289	-0.29855920002270864
2108	290-294	-0.028430765021678184
2108	295-299	1.3153295434728065
2108	300	1.5940915007690712
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	20.0
11	156.0
12	781.0
13	1290.0
14	1351.0
15	1551.0
16	2356.0
17	3409.0
18	5927.0
19	6947.0
20	6648.0
21	7272.0
22	9232.0
23	11520.0
24	14883.0
25	19327.0
26	24772.0
27	32979.0
28	44718.0
29	60959.0
30	82552.0
31	108760.0
32	130706.0
33	130653.0
34	94258.0
35	36682.0
36	4144.0
37	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.316506216553144	25.2815579540122	13.212960197943774	28.18897563149088
2	42.86186596134977	22.94543274130322	20.721883309870172	13.470817987476833
3	42.97906327469912	20.496376244851138	21.063758182481788	15.460802297967948
4	42.88438111760496	20.02675748569695	20.019765937175606	17.06909545952249
5	30.816494366470902	20.465092027738674	32.71274452644701	16.00566907934341
6	30.282885163223032	20.526001450450064	32.878645677801	16.312467708525897
7	16.587626618128727	47.07445169669477	20.372898387914812	15.965023297261682
8	29.110438026439905	20.997160720295398	33.43749555621916	16.45490569704554
9	16.54875834838294	20.56593622759742	33.46795026757486	29.417355156444785
10-14	25.664410411008255	34.81381150785187	23.34250529698676	16.179272784153124
15-19	22.979371376837353	33.40459972792211	22.286425967796216	21.329602927444316
20-24	24.69104465584991	31.289288947665295	26.16031265256981	17.859353743914983
25-29	18.174376330171732	28.625106058236042	35.30118169020093	17.899335921391295
30-34	19.215595656233855	29.674099038247327	30.199294683105098	20.91101062241372
35-39	21.078381183965416	30.050433950011612	28.53082680393802	20.340358062084952
40-44	19.666680886765352	30.621513113301006	28.345183415572905	21.366622584360737
45-49	21.598813095762885	29.150799406547883	26.74689172342856	22.503495774260674
50-54	22.290336494935275	30.087264005612198	26.282012997170202	21.34038650228233
55-59	20.96566320170262	27.403741781967966	29.77702885258024	21.85356616374918
60-64	20.77238836037522	27.115310780256813	29.384127525845027	22.728173333522935
65-69	18.289369528224526	27.30102526911537	30.191284027511152	24.218321175148954
70-74	19.870075698325344	26.396176689466227	30.807488303968828	22.9262593082396
75-79	21.35401409685783	26.041480027871394	28.320464144021155	24.284041731249616
80-84	22.532481075418666	27.855893519901027	25.806777299034454	23.804848105645853
85-89	21.090254966369468	26.941635974953666	26.93545023202461	25.032658826652256
90-94	21.548876849205335	29.95399798074599	27.61789646820149	20.87922870184719
95-99	22.778441382383193	27.623181604880337	28.98331034417379	20.61506666856268
100-104	22.360204579819783	27.3840469452858	27.999516516644622	22.25623195824979
105-109	24.962127137162334	27.473752067839353	25.52424763827861	22.039873156719707
110-114	21.106299977721847	26.745185311586063	30.050694651820887	22.09782005887121
115-119	23.983499945489623	27.56537690371571	27.404523887395776	21.04659926339889
120-124	20.08031985741981	28.005488958093373	27.514942953704097	24.39924823078272
125-129	20.007015248685825	25.957462944792837	29.613924320634787	24.42159748588655
130-134	22.786309836990267	29.429513340822584	26.658940413046466	21.12523640914068
135-139	22.60140115372401	26.863662433817293	26.108456692689447	24.426479719769254
140-144	21.690485331968205	28.432968824803645	26.193872085472275	23.68267375775588
145-149	21.860486611777084	26.364868772189283	29.684313809137837	22.0903308068958
150-154	21.916537500770257	29.204053676132514	25.68320464144021	23.19620418165702
155-159	21.155359318193668	27.537244808478974	30.413757471476856	20.89363840185051
160-164	22.843332432727085	30.137769056117246	26.227976622157755	20.79092188899791
165-169	21.238997198640558	28.68042224213036	27.185723968924346	22.894856590304737
170-174	21.30964738895288	30.813745147391323	26.70361522308965	21.17299224056615
175-179	20.05050505050505	28.564836539965587	28.411686077101372	22.972972332427986
180-184	21.57184230858562	28.1194156487446	28.35722309912831	21.95151894354147
185-189	21.600448407111944	26.95201664699553	29.266551009864006	22.180983936028518
190-194	20.527944863937357	27.740852921519277	27.798752423341817	23.932449791201552
195-199	19.891168844711782	31.848968331840222	26.87802473349165	21.381838089956346
200-204	22.54523176390844	27.886632633230473	27.475268878366016	22.092866724495067
205-209	22.26455071598197	27.04250387497689	29.860737833520563	20.832207575520574
210-214	24.22121259521541	27.072579383700923	28.97266897032265	19.733539050761014
215-219	22.801572742914836	28.296361076745875	29.25015049604444	19.65191568429485
220-224	21.287345534178005	28.229668813901572	28.462831032047365	22.02015461987306
225-229	25.339362655176828	26.91312467708526	28.195066573762023	19.55244609397589
230-234	23.082585593144017	27.324630632936593	28.208765268830966	21.384018505088427
235-239	23.539169261834676	27.507667003209	27.364968312880087	21.58819542207623
240-244	23.968284439894013	30.951348302357218	26.616825220767033	18.463542036981735
245-249	25.657276661500028	28.187624722115572	26.284596315098423	19.87050230128597
250-254	22.96593338357768	27.92848712370064	26.0269281268812	23.07865136584048
255-259	23.653569955775495	29.43783209855476	26.35413259768023	20.554465347989513
260-264	22.49171679251454	28.74199526944717	28.00565485924472	20.760633078793568
265-269	23.39696827495983	28.543387891111966	27.191127606425592	20.868516227502617
270-274	21.27708336295854	31.041811830174105	26.425493792926925	21.255611013940438
275-279	22.582749124278923	29.95001635311349	25.98879456223426	21.478439960373326
280-284	20.444022581516716	30.439282548620888	27.542695846309172	21.573999023553224
285-289	22.871440827799344	29.535595277031224	26.6132227957662	20.979741099403228
290-294	22.738032601946255	30.299475278358436	26.646592627352835	20.315899492342478
295-299	21.674985424398844	30.386383781503447	25.550412619863582	22.38821817423413
300	13.336556555702497	41.68740431058592	23.140129592499374	21.835909541212217
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.25
14	1.5
15	0.75
16	0.5
17	1.25
18	2.0
19	3.0
20	3.75
21	5.0
22	8.0
23	13.0
24	13.75
25	21.5
26	25.25
27	34.0
28	43.75
29	54.0
30	70.75
31	89.5
32	117.5
33	190.5
34	446.75
35	1081.25
36	2294.25
37	4256.5
38	7224.5
39	11978.75
40	15876.0
41	19930.0
42	26195.0
43	32149.75
44	33667.25
45	30757.5
46	28320.0
47	25031.0
48	16387.75
49	11804.75
50	8293.25
51	3312.0
52	989.5
53	279.0
54	91.0
55	38.25
56	28.25
57	17.25
58	11.5
59	10.0
60	11.0
61	5.75
62	6.0
63	7.0
64	6.0
65	4.25
66	6.25
67	3.75
68	3.0
69	1.75
70	3.5
71	2.75
72	2.0
73	3.5
74	3.0
75	2.5
76	1.75
77	1.25
78	1.0
79	1.5
80	1.75
81	1.0
82	1.25
83	1.5
84	2.25
85	1.5
86	0.75
87	1.25
88	2.0
89	1.25
90	0.75
91	0.25
92	0.5
93	0.5
94	0.0
95	0.75
96	1.0
97	1.25
98	0.5
99	0.5
100	0.6666666666666666
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	843876.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.212939079269777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.1638003648588	7.8674241906120805
2	2.2735029449196404	0.5098530003584871
3	1.3907710930563086	0.46783894618949473
4	1.0512458939510092	0.4715022466482065
5	1.0004039276546925	0.5608734147727137
6	0.8751698965412051	0.588793604035642
7	0.7055744647505854	0.5538094442395687
8	0.808808663705106	0.7255297818328758
9	0.979263493057912	0.988237970119016
>10	18.667498306393668	45.20540383728597
>50	1.736234727791968	13.160668111376056
>100	0.31932416164696625	4.887762620847903
>500	0.002104165251814389	0.13295092242677115
>1k	0.008415326860159536	2.9901677950945516
>5k	0.010519158564854124	8.280020076848597
>10k+	0.007363410995397887	12.609164037312052
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	23896	2.8316956519678245	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	19798	2.3460792817902156	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	15699	1.8603444107902107	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	13875	1.6441989107404404	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	12398	1.4691731960619807	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATACTCCTC	10862	1.287155932862174	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	10374	1.2293275315330687	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	8878	1.0520503012290905	No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAGTA	8326	0.98663784726666	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	8241	0.976565277363025	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	7462	0.8842531367167688	No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	7236	0.857471950855339	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG	6712	0.7953775199199882	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA	6504	0.7707293488616811	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTCAAATT	5964	0.706738904767999	No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	5834	0.6913337978565571	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT	5042	0.5974811465191568	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAGTA	4857	0.575558494375951	No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	4788	0.5673819376306471	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	4594	0.5443927780858799	No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	3787	0.44876261441254406	No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	3188	0.37778062179751526	No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	1668	0.19765937175604	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1263	0.14966653868577848	No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	1206	0.14291199180922315	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0010665074015613668	0.0	0.0
2	0.0013035090463527816	0.0	0.0
3	0.002488517270309856	0.0	0.0
4	0.0035550246718712227	0.0	0.0
5	0.004621532073432589	0.0	0.0
6	0.006517545231763909	0.0	0.0
7	0.008058055922908106	0.0	0.0
8	0.010309571548426546	0.0	0.0
9	0.013390592930714939	0.0	0.0
10-14	0.02265735724205926	0.0	0.0
15-19	0.03481554161985884	0.0	0.0
20-24	0.04744772928724125	0.0	0.0
25-29	0.05842090544108376	0.0	0.0
30-34	0.06906227929221828	0.0	0.0
35-39	0.07716773554408467	0.0	0.0
40-44	0.08728770577667809	0.0	0.0
45-49	0.0972891751868758	0.0	0.0
50-54	0.10575013390592931	0.0	0.0
55-59	0.11857192288914484	0.0	0.0
60-64	0.13184401499746407	0.0	0.0
65-69	0.14788902634984286	0.0	0.0
70-74	0.16838966862430027	0.0	0.0
75-79	0.19363034379458594	0.0	0.0
80-84	0.22228384264986797	0.0	0.0
85-89	0.262147519303784	0.0	0.0
90-94	0.31388497836174983	0.0	0.0
95-99	0.3695092643942949	0.0	0.0
100-104	0.4396143509235954	0.0	0.0
105-109	0.5235129331797562	0.0	0.0
110-114	0.6150192682337214	0.0	0.0
115-119	0.7311974745104731	0.0	0.0
120-124	0.8649849029952268	0.0	0.0
125-129	1.0288478402040109	0.0	0.0
130-134	1.2281425233091117	0.0	0.0
135-139	1.4533177860254352	0.0	0.0
140-144	1.7118391801639106	0.0	0.0
145-149	2.0352990249752336	0.0	0.0
150-154	2.4254511326308603	0.0	0.0
155-159	2.8834331110257905	0.0	0.0
160-164	3.3630533395901763	0.0	0.0
165-169	3.881257435926605	0.0	0.0
170-174	4.484876925045859	0.0	0.0
175-179	5.202731206954576	0.0	0.0
180-184	6.017163659115794	0.0	0.0
185-189	6.9267759718252435	0.0	0.0
190-194	7.929198128635012	0.0	0.0
195-199	8.994686423123776	0.0	0.0
200-204	10.133977029800587	9.480065791656594E-5	4.740032895828297E-5
205-209	11.350933075475544	1.1850082239570742E-4	1.1850082239570742E-4
210-214	12.607563196488583	1.1850082239570742E-4	1.1850082239570742E-4
215-219	13.87590119875432	1.1850082239570742E-4	1.1850082239570742E-4
220-224	15.19874957932208	1.1850082239570742E-4	1.1850082239570742E-4
225-229	16.575847636382594	1.1850082239570742E-4	1.1850082239570742E-4
230-234	17.917134744915128	1.1850082239570742E-4	1.1850082239570742E-4
235-239	19.224980921367596	1.1850082239570742E-4	1.1850082239570742E-4
240-244	20.445752693523694	1.1850082239570742E-4	1.1850082239570742E-4
245-249	21.597462186387578	1.1850082239570742E-4	1.1850082239570742E-4
250-254	22.699093231707028	1.1850082239570742E-4	1.1850082239570742E-4
255-259	23.800582076039607	1.1850082239570742E-4	1.1850082239570742E-4
260-264	24.86775308220639	1.1850082239570742E-4	1.1850082239570742E-4
265-269	25.872521555299596	1.1850082239570742E-4	1.1850082239570742E-4
270-274	26.716887315197965	1.1850082239570742E-4	2.1330148031227335E-4
275-279	27.460503675895513	1.1850082239570742E-4	2.3700164479141484E-4
280-284	28.15020216240301	1.1850082239570742E-4	2.3700164479141484E-4
285-288	28.75766107816788	1.1850082239570742E-4	3.5550246718712227E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTTAT	10570	0.0	289.68875	2
CGGGTTA	10580	0.0	288.9981	1
GGGGGGA	11260	0.0	286.95026	1
GGTTATT	10990	0.0	278.21655	3
GGGGGAG	11650	0.0	277.84894	2
ATTAGTA	10995	0.0	275.8172	7
TATTAGT	11130	0.0	272.07547	6
GTTATTA	11430	0.0	266.6063	4
TTATTAG	11405	0.0	266.41736	5
GGAGCGA	8550	0.0	257.37894	5
GAGCGAA	8570	0.0	256.94983	6
TTAGTAG	11880	0.0	255.39394	8
TAGTAGA	11950	0.0	253.52887	9
GCGAAAG	8720	0.0	252.19267	8
CGAAAGC	8755	0.0	251.85608	9
GGGGAGC	13240	0.0	243.92674	3
AGCGAAA	9560	0.0	231.10982	7
GGGAGCG	9755	0.0	225.88724	4
GAGCAAA	5895	0.0	174.80406	6
GCAAAAG	6115	0.0	168.27473	8
>>END_MODULE