FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n02 clinical flu pool d3.3420000000b6f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n02 clinical flu pool d3.3420000000b6f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843876
Sequences flagged as poor quality0
Sequence length300
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC238962.8316956519678245No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA197982.3460792817902156No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG156991.8603444107902107No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG138751.6441989107404404No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA123981.4691731960619807No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATACTCCTC108621.287155932862174No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG103741.2293275315330687No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG88781.0520503012290905No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAGTA83260.98663784726666No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA82410.976565277363025No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG74620.8842531367167688No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC72360.857471950855339No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG67120.7953775199199882No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA65040.7707293488616811No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTCAAATT59640.706738904767999No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA58340.6913337978565571No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT50420.5974811465191568Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAGTA48570.575558494375951No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA47880.5673819376306471No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT45940.5443927780858799No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC37870.44876261441254406No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA31880.37778062179751526No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA16680.19765937175604No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT12630.14966653868577848No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA12060.14291199180922315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT105700.0289.688752
CGGGTTA105800.0288.99811
GGGGGGA112600.0286.950261
GGTTATT109900.0278.216553
GGGGGAG116500.0277.848942
ATTAGTA109950.0275.81727
TATTAGT111300.0272.075476
GTTATTA114300.0266.60634
TTATTAG114050.0266.417365
GGAGCGA85500.0257.378945
GAGCGAA85700.0256.949836
TTAGTAG118800.0255.393948
TAGTAGA119500.0253.528879
GCGAAAG87200.0252.192678
CGAAAGC87550.0251.856089
GGGGAGC132400.0243.926743
AGCGAAA95600.0231.109827
GGGAGCG97550.0225.887244
GAGCAAA58950.0174.804066
GCAAAAG61150.0168.274738
Produced by FastQC (version 0.11.2)