FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n02 clinical flu pool d2.3420000000b68b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n02 clinical flu pool d2.3420000000b68b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938181
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT929949.912159807116112Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC393234.191408694057969No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA379404.043995774802517No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG297323.16911129089163No Hit
GGGGGGAGCGAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACA273472.9148959529131373No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA191602.042249843047344No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTTAATTGTCGTACTCTTC181001.929265248390236No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAACATTAATTG141661.5099431772760268No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT139031.4819102070922348No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG119981.2788577044301686No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA100421.070369150515732No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA97711.04148346641No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG78850.840456159312542No Hit
GGGGGGAGCAAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACA76270.8129561353299629No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA73590.7843902189449584No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA70690.7534793392746175No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT64940.6921905261351488No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG46130.4916961652389038No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT40090.4273162641324009No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT34170.3642154339088087No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT29700.31657004352038676No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA27170.2896029657390205No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA17960.19143427547562783No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT14660.15625982619558487No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT11350.12097878767529932No Hit
GGGGGGAGCGAAAGCAGGCAGCGGAGGCCATGGAAATTGCTAGTCAGGCC10910.11628886110462693No Hit
GGGGGAGCGAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACAA9920.10573652632061405No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT9510.1013663674706693No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA172900.0290.34211
GGGGGGA169150.0289.913481
GGGTTAT173600.0289.002042
TTAGTAG176150.0283.983868
TTATTAG176350.0282.82825
TAGTAGA178300.0280.147259
TATTAGT179400.0278.429536
GGTTATT180200.0277.68293
GTTATTA180250.0276.953434
ATTAGTA182500.0274.263857
GGGGAGC178500.0273.9043
GGGAGCG136900.0269.623384
GGAGCGA136800.0269.39075
AGCGAAA142100.0259.756847
GAGCGAA142300.0258.771946
GGGGGAG190250.0258.532782
CGAAAGC149200.0248.3819
GCGAAAG150250.0246.253888
GGGAGCA51800.0231.565984
GCAAAAG65150.0175.992658
Produced by FastQC (version 0.11.2)