##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE77A l01n02 clinical flu pool d1.3420000000b5e9.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	546802
Sequences flagged as poor quality	0
Sequence length	300
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.056343978259044	34.0	34.0	34.0	34.0	34.0
2	33.376549463974165	34.0	34.0	34.0	34.0	34.0
3	33.3699876737832	34.0	34.0	34.0	34.0	34.0
4	33.35815157954799	34.0	34.0	34.0	34.0	34.0
5	33.42517035416842	34.0	34.0	34.0	34.0	34.0
6	36.85600455009309	38.0	38.0	38.0	36.0	38.0
7	36.94370357094524	38.0	38.0	38.0	36.0	38.0
8	36.90996192406026	38.0	38.0	38.0	36.0	38.0
9	36.84122589163902	38.0	38.0	38.0	36.0	38.0
10-14	36.874127380660646	38.0	38.0	38.0	35.8	38.0
15-19	36.840093489050886	38.0	38.0	38.0	35.6	38.0
20-24	36.88755564171309	38.0	38.0	38.0	35.8	38.0
25-29	36.75881031890886	38.0	38.0	38.0	35.6	38.0
30-34	36.86785527485269	38.0	38.0	38.0	35.8	38.0
35-39	36.7555488092582	38.0	38.0	38.0	35.4	38.0
40-44	36.70398133145087	38.0	38.0	38.0	35.0	38.0
45-49	36.46090614152838	38.0	37.4	38.0	34.2	38.0
50-54	36.25891748750005	38.0	37.0	38.0	33.8	38.0
55-59	36.164323832026945	38.0	37.0	38.0	33.6	38.0
60-64	35.844577744777816	38.0	37.0	38.0	31.4	38.0
65-69	35.53764433926723	38.0	37.0	38.0	29.8	38.0
70-74	36.09850914956419	38.0	37.0	38.0	33.6	38.0
75-79	35.90225273499365	38.0	37.0	38.0	32.0	38.0
80-84	32.83482576874262	38.0	33.8	38.0	12.6	38.0
85-89	32.73045672839529	38.0	34.0	38.0	11.0	38.0
90-94	32.749120156839226	38.0	33.6	38.0	11.0	38.0
95-99	32.57483513227823	38.0	33.2	38.0	11.0	38.0
100-104	32.60868833691172	38.0	33.0	38.0	11.0	38.0
105-109	32.43380967882341	38.0	31.8	38.0	11.0	38.0
110-114	32.315600893925044	38.0	31.8	38.0	11.0	38.0
115-119	32.35030303473652	38.0	32.0	38.0	11.0	38.0
120-124	32.13559057940534	38.0	31.0	38.0	11.0	38.0
125-129	32.01653285832897	38.0	30.4	38.0	11.0	38.0
130-134	31.96855936883918	38.0	30.0	38.0	11.0	38.0
135-139	31.672249187091488	37.4	28.0	38.0	11.0	38.0
140-144	31.565888932374058	37.0	27.8	38.0	11.0	38.0
145-149	31.391214735864168	37.0	26.6	38.0	11.0	38.0
150-154	31.159188883727563	37.0	25.4	38.0	11.0	38.0
155-159	30.971271136535712	37.0	24.2	38.0	11.0	38.0
160-164	30.74430488549786	37.0	23.8	38.0	11.0	38.0
165-169	30.15552174278806	36.6	22.2	38.0	11.0	38.0
170-174	29.52346992147066	35.6	21.4	38.0	11.0	38.0
175-179	29.70525089520521	35.8	21.8	38.0	11.0	38.0
180-184	29.763233126433335	36.0	21.8	38.0	11.0	38.0
185-189	29.461947103339053	35.4	21.4	38.0	10.4	38.0
190-194	28.861746665154847	35.0	20.2	37.8	10.0	38.0
195-199	28.482840589463827	34.6	19.6	37.2	10.0	38.0
200-204	27.880908628717528	33.8	18.6	37.0	10.0	37.8
205-209	27.275031181305117	32.4	17.6	37.0	9.4	37.2
210-214	26.776549098211053	31.6	16.6	37.0	9.0	37.0
215-219	25.941857930292866	29.8	15.6	37.0	9.0	37.0
220-224	24.927306776493133	27.6	14.4	36.0	8.8	37.0
225-229	24.15182643808911	26.4	13.2	34.8	8.2	37.0
230-234	23.352588688410066	24.6	11.8	33.8	8.0	37.0
235-239	22.32250247804507	22.8	10.2	32.6	8.0	37.0
240-244	21.591085987249496	21.6	9.6	32.0	8.0	36.4
245-249	20.74443070800765	20.4	9.2	30.8	8.0	36.2
250-254	19.72055552101126	18.8	8.8	29.2	7.4	35.8
255-259	18.774865856379456	17.6	8.0	27.6	7.0	34.2
260-264	18.204716149538587	16.4	8.2	26.4	7.4	33.6
265-269	17.658269355269365	15.6	8.0	25.2	7.0	32.6
270-274	16.751990665725437	14.0	8.0	23.8	7.0	31.2
275-279	15.815277193572811	13.0	8.0	22.0	7.0	30.0
280-284	15.091278744408395	12.4	8.0	20.6	7.0	28.6
285-289	14.685480301827718	12.2	8.0	19.8	7.0	27.4
290-294	14.210875600308702	12.0	8.0	19.0	7.0	26.8
295-299	13.645963986964201	11.2	8.0	18.8	7.0	25.2
300	12.064979645282936	10.0	8.0	13.0	7.0	21.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.073714070279145
1101	2	0.020526226618265753
1101	3	0.040258096003391586
1101	4	0.12565118148251742
1101	5	0.07657137832535454
1101	6	0.34325810919750666
1101	7	0.13876273545115936
1101	8	0.046255417361642515
1101	9	0.15362263133791032
1101	10-14	0.15843102182332558
1101	15-19	0.1442917603298426
1101	20-24	0.08315733319255258
1101	25-29	0.006551970445592303
1101	30-34	0.13963937006017346
1101	35-39	0.056810141052096697
1101	40-44	0.16618458048266405
1101	45-49	0.05768287797840088
1101	50-54	0.09879697945235222
1101	55-59	0.08995825597727247
1101	60-64	0.07386928211202815
1101	65-69	0.1881856855499251
1101	70-74	0.1184140383615997
1101	75-79	0.20003318057732145
1101	80-84	-0.12908414180716932
1101	85-89	-0.12170556109342101
1101	90-94	-0.17804536271963656
1101	95-99	-0.06318127415494956
1101	100-104	-0.057573475241213146
1101	105-109	-0.017374221845301463
1101	110-114	0.02894151919162624
1101	115-119	-0.008318881331611294
1101	120-124	-0.043504903862533695
1101	125-129	-0.061633803344999905
1101	130-134	-0.151734730184387
1101	135-139	-0.2611006439304546
1101	140-144	-0.23401754095419136
1101	145-149	-0.16197402159399132
1101	150-154	-0.04918415553503408
1101	155-159	-0.021606798988234033
1101	160-164	5.698560329925328E-4
1101	165-169	0.04218888192036374
1101	170-174	0.859851955551207
1101	175-179	-0.14630791004608312
1101	180-184	-0.5423522304952009
1101	185-189	-0.41704243141444763
1101	190-194	-0.9081031247301503
1101	195-199	-1.0139950396857316
1101	200-204	-0.8880656486311125
1101	205-209	-0.5794242274656014
1101	210-214	-1.3192590677767306
1101	215-219	-1.0559530842038036
1101	220-224	-1.3938587296775538
1101	225-229	-1.0530679786768147
1101	230-234	-1.064913595539437
1101	235-239	-1.0858601405379424
1101	240-244	-1.1010648390751108
1101	245-249	-1.0690318362977003
1101	250-254	-0.7487912058175255
1101	255-259	-0.6051292860801283
1101	260-264	-0.39013124844208846
1101	265-269	-0.879757818839888
1101	270-274	-0.5211486348998946
1101	275-279	-0.44686394487737147
1101	280-284	-0.13252691041270914
1101	285-289	-0.47684618812916035
1101	290-294	-0.341078138947676
1101	295-299	-0.5054193363629746
1101	300	0.418491782353021
1102	1	0.14380068704701898
1102	2	0.11845495608208978
1102	3	0.04370158326978668
1102	4	0.21168422766243822
1102	5	0.12971358987739734
1102	6	0.33785868350433645
1102	7	0.2953390194035208
1102	8	0.21360701553516037
1102	9	0.24962131325666093
1102	10-14	0.14342278381550244
1102	15-19	0.13783692814983084
1102	20-24	0.028406121028545783
1102	25-29	0.08851779448175279
1102	30-34	0.14337424743154514
1102	35-39	0.17052571794801707
1102	40-44	0.20367504793076563
1102	45-49	0.08086557345455958
1102	50-54	0.10742947015517501
1102	55-59	0.299092088298508
1102	60-64	0.34555124793606495
1102	65-69	0.4393898469207258
1102	70-74	0.302606948967437
1102	75-79	0.45776937602993684
1102	80-84	0.2901466636346157
1102	85-89	0.12280745495892376
1102	90-94	0.17917019063910544
1102	95-99	0.08577650017071647
1102	100-104	0.24933797794335533
1102	105-109	0.19643926965778746
1102	110-114	0.20011602373428872
1102	115-119	0.18835767094443412
1102	120-124	0.09578474449222085
1102	125-129	0.29821320508130356
1102	130-134	0.12614692494906166
1102	135-139	0.14326879541427573
1102	140-144	0.16510075683802583
1102	145-149	0.16550797247464644
1102	150-154	0.29721512893514657
1102	155-159	0.20483673535249736
1102	160-164	0.24562281465465574
1102	165-169	0.4944738699164084
1102	170-174	1.188735352434886
1102	175-179	0.730998026026981
1102	180-184	0.4009740416885812
1102	185-189	0.39659829729619034
1102	190-194	0.149296960003646
1102	195-199	0.012056836226328471
1102	200-204	0.41757620986345145
1102	205-209	0.060949542717523286
1102	210-214	0.2973120379992906
1102	215-219	0.2307107580030454
1102	220-224	0.08096215249539895
1102	225-229	0.15252115656773668
1102	230-234	-0.3469279061358712
1102	235-239	-0.30295942030068446
1102	240-244	-0.01614086411865756
1102	245-249	-0.044742894057904437
1102	250-254	0.4574743875544094
1102	255-259	0.7071895954337784
1102	260-264	0.46973694343288486
1102	265-269	0.1567309068579874
1102	270-274	0.1219686272570506
1102	275-279	0.43846392783363974
1102	280-284	0.6334397609613873
1102	285-289	0.20595473452771706
1102	290-294	0.2726694484928913
1102	295-299	-0.022524265045362313
1102	300	1.10263244045216
1103	1	-0.28236787930244844
1103	2	-0.05118275745398648
1103	3	-0.06833106148794599
1103	4	-0.20557927846439839
1103	5	-0.08806801155996169
1103	6	0.034078964321501815
1103	7	-0.15053903331861562
1103	8	-0.1840938771097882
1103	9	-0.09095933374456422
1103	10-14	-0.04773575334952085
1103	15-19	-0.2889439412996495
1103	20-24	-0.34663936678908414
1103	25-29	-0.30875426734745304
1103	30-34	-0.28267026123348415
1103	35-39	-0.377380891807384
1103	40-44	-0.26994382404740236
1103	45-49	-0.3924287522134975
1103	50-54	-0.3959494619030366
1103	55-59	-0.3540522273878963
1103	60-64	-0.44690509421597824
1103	65-69	-0.21470301096906752
1103	70-74	-0.337616925244717
1103	75-79	-0.2347659637740449
1103	80-84	0.026158375440537895
1103	85-89	-0.0011845407061841229
1103	90-94	-0.031159298965711457
1103	95-99	-0.11946120576029529
1103	100-104	-0.13153185179816518
1103	105-109	-0.10347384243141988
1103	110-114	0.0026810233065148736
1103	115-119	-0.16663515177189936
1103	120-124	-0.028054579417165826
1103	125-129	-0.07444058366889905
1103	130-134	-0.12182508839782713
1103	135-139	-0.08670943507634732
1103	140-144	-0.03914981116554728
1103	145-149	-0.10336638228280037
1103	150-154	-0.06492341640532828
1103	155-159	-0.10106631903325436
1103	160-164	0.12241822879818187
1103	165-169	0.4279341762105524
1103	170-174	1.184074490970719
1103	175-179	0.5869976647862067
1103	180-184	0.22120238387865143
1103	185-189	0.07572418970347528
1103	190-194	0.08891870289527759
1103	195-199	0.26476965932292984
1103	200-204	0.28163322886661746
1103	205-209	0.5798485188851323
1103	210-214	0.4228487181966436
1103	215-219	0.16428162298455007
1103	220-224	0.29414420833562005
1103	225-229	0.5036784579090785
1103	230-234	0.47762249170428817
1103	235-239	0.5262854914329864
1103	240-244	0.6041057302364834
1103	245-249	0.6554003664252086
1103	250-254	1.1875212537907522
1103	255-259	1.3093470583828335
1103	260-264	0.7927825833518867
1103	265-269	0.7705087247736238
1103	270-274	0.9723812973016379
1103	275-279	0.9269734294164405
1103	280-284	1.1848417553906785
1103	285-289	0.7926817981155985
1103	290-294	0.6527871369322362
1103	295-299	0.5251184488234308
1103	300	1.223511206149496
2116	1	0.029298087748230728
2116	2	-0.06996026213811746
2116	3	0.10739928342509586
2116	4	0.055311528204619265
2116	5	-0.027084111207891226
2116	6	0.10620489708392711
2116	7	0.010836699099229463
2116	8	0.02941902903849325
2116	9	0.08679919085247434
2116	10-14	0.08755301844987429
2116	15-19	0.06556358793463346
2116	20-24	-0.03446138448160241
2116	25-29	0.10347822376129301
2116	30-34	0.023950650076407953
2116	35-39	-0.04007439922967393
2116	40-44	0.06981655394309882
2116	45-49	0.07800076489129282
2116	50-54	0.1429601642005025
2116	55-59	0.16094768074334098
2116	60-64	0.05836379152895432
2116	65-69	0.25431540188728974
2116	70-74	-0.018287972402603714
2116	75-79	0.1251529655732213
2116	80-84	0.23115829770542717
2116	85-89	0.19959818118161365
2116	90-94	0.1436144800866117
2116	95-99	0.1307373014429274
2116	100-104	0.22387024365772845
2116	105-109	0.3002114613456115
2116	110-114	0.18376378387341674
2116	115-119	0.2626736229201754
2116	120-124	0.29372957797900057
2116	125-129	0.31566142835130506
2116	130-134	0.2817927507263036
2116	135-139	0.25148983345982145
2116	140-144	0.31572161260716314
2116	145-149	0.37098277462104434
2116	150-154	0.3110837597231182
2116	155-159	0.36331323936536464
2116	160-164	0.3524220967087963
2116	165-169	0.6889030006625312
2116	170-174	1.407069290256313
2116	175-179	1.1109086547875897
2116	180-184	0.6178126619677613
2116	185-189	0.722954629076451
2116	190-194	0.8549483656709569
2116	195-199	0.5271649465965531
2116	200-204	0.7907215938993879
2116	205-209	0.7374016626107576
2116	210-214	0.857426425745853
2116	215-219	0.9332018069293788
2116	220-224	1.2362149680149344
2116	225-229	0.2833451203356461
2116	230-234	0.6356246145794735
2116	235-239	0.6413131015306348
2116	240-244	0.718297287449758
2116	245-249	0.6663807098071004
2116	250-254	0.9944891463636587
2116	255-259	1.0853717602451312
2116	260-264	1.1620936100065506
2116	265-269	0.7017661066645466
2116	270-274	0.9490104485331727
2116	275-279	1.0502462377221367
2116	280-284	1.1146698477286812
2116	285-289	0.8413083600612943
2116	290-294	0.8463877942414832
2116	295-299	0.618909580707875
2116	300	0.7194037300577545
1117	1	0.0052629971001678655
1117	2	-0.050268376502685896
1117	3	-0.008615206909119877
1117	4	0.042792469297289415
1117	5	-0.09101213809625364
1117	6	-0.013019001951164455
1117	7	0.07133273150771657
1117	8	0.02139202938350593
1117	9	0.021303325383314586
1117	10-14	0.013074703869392579
1117	15-19	-0.08445366246323971
1117	20-24	0.006295184581205149
1117	25-29	0.0895343376361808
1117	30-34	0.17153503790986946
1117	35-39	0.0012394689772818879
1117	40-44	0.20916610265043545
1117	45-49	0.2844343286009092
1117	50-54	0.24000339035878682
1117	55-59	0.3263542336686669
1117	60-64	0.2677082207633745
1117	65-69	0.45130353806526813
1117	70-74	0.3513504926458637
1117	75-79	0.3519803948187672
1117	80-84	0.5101361838082852
1117	85-89	0.5539366562464494
1117	90-94	0.5567923928997587
1117	95-99	0.567707408172808
1117	100-104	0.6367457180436702
1117	105-109	0.48693032437745387
1117	110-114	0.4835361899143962
1117	115-119	0.46325624119670294
1117	120-124	0.5200013774737329
1117	125-129	0.5524904713437522
1117	130-134	0.6468943611344073
1117	135-139	0.42463903270748204
1117	140-144	0.6087986384159834
1117	145-149	0.8075769896166562
1117	150-154	0.6294615545658075
1117	155-159	0.8720363760441607
1117	160-164	0.7714982544330802
1117	165-169	1.1128206773121683
1117	170-174	1.6012628619790163
1117	175-179	1.1220254232872477
1117	180-184	0.9112416515396156
1117	185-189	0.9094656526371558
1117	190-194	1.0955085027837903
1117	195-199	1.1587264971290914
1117	200-204	1.1676903651145913
1117	205-209	0.8341975403221298
1117	210-214	0.6557606494223016
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2108	210-214	-0.10993907811472425
2108	215-219	-0.3956264801394376
2108	220-224	-0.7246569569028551
2108	225-229	-0.544773077797128
2108	230-234	-0.5272148523716105
2108	235-239	-0.5990448173320111
2108	240-244	-0.7687794721241517
2108	245-249	0.05411924201261442
2108	250-254	-0.26252484929070974
2108	255-259	0.4075272914691901
2108	260-264	-0.2596498616192058
2108	265-269	-0.6332671852548053
2108	270-274	-0.2321337118126614
2108	275-279	-0.2179496257970719
2108	280-284	-0.12457641792211582
2108	285-289	-0.4947427890346905
2108	290-294	-0.45898316340469236
2108	295-299	1.0366267251480963
2108	300	1.718065340059134
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	26.0
10	223.0
11	304.0
12	624.0
13	980.0
14	1058.0
15	1093.0
16	2239.0
17	7975.0
18	40843.0
19	41968.0
20	13721.0
21	5421.0
22	4388.0
23	5059.0
24	6552.0
25	8576.0
26	11576.0
27	15360.0
28	21020.0
29	29233.0
30	40702.0
31	55935.0
32	71144.0
33	75884.0
34	58196.0
35	23885.0
36	2809.0
37	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.596040248572606	24.35122768387826	10.3726760326407	22.68005603490843
2	30.7219066499391	39.24400422822155	19.05936701036207	10.97472211147728
3	29.7930512324388	18.840457789108306	37.65951843628955	13.706972542163342
4	30.464226539039725	18.26383224640729	18.02005113368276	33.251890080870226
5	40.929623520031015	18.776815007991924	26.35103748706113	13.94252398491593
6	40.69663241904748	18.603809057026126	26.585308758929195	14.114249764997203
7	14.556823127932963	53.10788914451666	18.269318693055254	14.065969034495119
8	21.298751650506034	37.209446929601576	26.92711438509735	14.564687034795046
9	32.596076824883596	18.419464449654537	27.14675513257084	21.837703592891028
10-14	27.58471988032231	30.680429113280493	23.866811021174026	17.86803998522317
15-19	25.33260668395507	26.718227073053868	26.828614379610897	21.120551863380165
20-24	30.241440228821403	32.36824298374915	22.135581069564488	15.254735717864968
25-29	23.309205160185954	32.681482511036904	28.96130592060746	15.048006408169684
30-34	19.99583030054755	27.19353623432248	35.798954649032005	17.011678816097966
35-39	20.751496885527118	31.72230533172885	30.542755878727583	16.983441904016445
40-44	24.12705147384245	27.728281900944037	30.698168624108906	17.446498001104604
45-49	20.446413875589336	32.08078975570682	26.280554935790285	21.19224143291356
50-54	25.07273199439651	23.33678369866972	31.095131327244594	20.495352979689173
55-59	28.84510298060358	21.60259106587028	28.02871971938654	21.523586234139596
60-64	21.159651939824652	25.962962827495144	27.259483323031002	25.6179019096492
65-69	16.86292295931617	25.18202201162395	35.747930695205945	22.207124333853937
70-74	17.347632232508296	39.709437785523825	23.57463944901445	19.368290532953427
75-79	16.680151133316993	39.670374285390324	25.203638611416928	18.445835969875752
80-84	19.7316030299816	33.586234139597146	25.36479383762312	21.317368992798123
85-89	18.776229787016142	33.08009114816698	26.004915856196575	22.138763208620304
90-94	18.441958880911187	31.493410777575797	28.635776752828264	21.428853588684753
95-99	20.296121813746108	30.0978050555777	27.612298418806073	21.99377471187011
100-104	20.63778113467032	29.475898039875492	27.52963595597675	22.356684869477434
105-109	21.30913932282618	28.78402054125625	25.785494566589	24.12134556932857
110-114	19.457536731760307	28.89565144238682	29.42812937772722	22.218682448125644
115-119	19.46796098039144	31.479658084644903	26.49291699737748	22.559463937586184
120-124	17.968807721990775	30.627283733417215	27.825062819814118	23.578845724777892
125-129	18.996163144977523	28.287606848548467	29.44627122797649	23.269958778497518
130-134	21.202007307946936	29.473484003350393	27.081246959594154	22.243261729108525
135-139	20.940486684393985	28.69876116034689	26.82521278268916	23.53553937256996
140-144	19.428538823390852	29.910325825555073	27.324745456214668	23.33638989483941
145-149	18.845256178747032	28.930555824797942	28.95451331725682	23.2696746791982
150-154	20.412603186458558	27.629921942409077	27.275314218288393	24.682160652843976
155-159	19.807352570034492	28.00110460459179	29.606256012231118	22.5852868131426
160-164	21.185059145788582	28.819787220734987	27.66477920502764	22.330374428448792
165-169	18.283663294451483	27.574854362220464	28.99438151081434	25.14710083251372
170-174	19.362145712707708	29.450660385294857	27.239658962476366	23.94753493952107
175-179	19.379965464634534	29.10926774564385	28.39573680993735	23.115029979784264
180-184	19.42726575781783	28.644539445385675	28.135177365976983	23.793017430819514
185-189	20.6390032509049	28.054563117591464	28.29267507629824	23.0137585552054
190-194	18.955270829294697	29.491955040398537	28.228170343195526	23.32460378711124
195-199	19.563388575755024	29.51291326659376	27.993204121418724	22.930494036232492
200-204	20.761153031627536	28.796090723881772	28.245032022560267	22.19772422193042
205-209	20.368030841145423	28.568147153814362	28.431168869170193	22.632653135870022
210-214	20.875981343141646	28.42453277386167	28.81703345418155	21.88245242881513
215-219	21.41788852926234	27.977449964502675	28.844711191514	21.75995031472098
220-224	19.279636606902173	28.47675336840515	28.192299293820906	24.051310730871773
225-229	21.04710663091942	29.216864605469624	27.540572272961693	22.195456490649264
230-234	20.365456269863245	28.69070927762804	28.388186277504047	22.555648175004674
235-239	20.864627415408137	28.766573640915727	27.177771844287328	23.19102709938881
240-244	21.960571453861345	30.70458070913446	25.63608971294535	21.698758124058845
245-249	20.897378500721288	29.60624109366176	26.48119537323958	23.015185032377374
250-254	21.1678816097966	29.024912125412854	26.791050508227844	23.016155756562704
255-259	21.395349687821184	28.55892992344578	28.013028481973368	22.03269190675967
260-264	21.309285628070125	29.93529650586501	27.288854100753106	21.466563765311758
265-269	21.427304319264728	29.723881467750445	26.954685924359374	21.89412828862545
270-274	20.921116543916476	30.844789042314435	26.19904550389026	22.035048909878828
275-279	20.962513290921866	30.592949767173405	25.475483072659966	22.96905386924476
280-284	19.52667310166133	31.128024816304556	27.04489999850037	22.30040208353374
285-289	21.387961273001928	30.389866898804318	26.635454881291583	21.58671694690217
290-294	20.558117929341883	31.684119663059025	26.37056923712788	21.387193170471214
295-299	20.13438136656413	32.797173382686964	24.71549116499208	22.352954085756817
300	12.115537251143923	44.63425517829123	23.136162632909173	20.114044937655677
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.25
7	0.25
8	0.0
9	0.25
10	0.75
11	1.25
12	2.0
13	2.25
14	2.75
15	5.5
16	3.5
17	5.75
18	7.25
19	8.25
20	11.25
21	11.25
22	16.0
23	18.5
24	20.25
25	22.25
26	25.75
27	38.25
28	46.0
29	67.0
30	98.75
31	147.5
32	253.25
33	453.25
34	803.25
35	1355.0
36	2219.75
37	3782.25
38	6406.75
39	10167.5
40	14234.25
41	18460.5
42	23778.75
43	24230.0
44	20683.25
45	16157.5
46	11174.75
47	8022.5
48	6811.75
49	5452.75
50	3855.25
51	1882.0
52	579.5
53	248.0
54	151.25
55	111.75
56	93.25
57	72.5
58	56.5
59	47.0
60	41.5
61	34.25
62	31.5
63	26.5
64	22.25
65	22.0
66	18.25
67	13.25
68	14.25
69	9.75
70	10.75
71	9.75
72	3.5
73	4.75
74	3.0
75	4.0
76	4.5
77	3.25
78	1.5
79	1.5
80	0.75
81	2.0
82	1.75
83	0.25
84	1.5
85	1.25
86	0.75
87	0.5
88	0.5
89	0.5
90	0.75
91	0.75
92	1.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.25
100	0.3333333333333333
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	3.6576310986426535E-5
145-149	3.6576310986426535E-5
150-154	1.097289329592796E-4
155-159	0.0
160-164	1.097289329592796E-4
165-169	3.6576310986426535E-5
170-174	0.0
175-179	3.6576310986426535E-5
180-184	7.315262197285307E-5
185-189	7.315262197285307E-5
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	7.315262197285307E-5
215-219	3.6576310986426535E-5
220-224	3.6576310986426535E-5
225-229	0.0
230-234	1.097289329592796E-4
235-239	0.0
240-244	3.6576310986426535E-5
245-249	7.315262197285307E-5
250-254	0.0
255-259	0.0
260-264	0.0
265-269	1.097289329592796E-4
270-274	7.315262197285307E-5
275-279	3.6576310986426535E-5
280-284	3.6576310986426535E-5
285-289	0.0
290-294	0.0
295-299	0.0
300	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	546802.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.075533505696917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.10234419240471	8.38172349281504
2	2.843556037963532	0.7436202449943787
3	2.0506412786572445	0.8043968624174394
4	1.6426903264546728	0.8591620961216913
5	1.7325262419669145	1.1326852463168777
6	1.5299232296616263	1.200273747035078
7	1.7230040682674266	1.5770438197557985
8	1.712816258508214	1.791678910178127
9	1.7762108409811925	2.0902413927988515
>10	20.670253522245225	48.31883599188206
>50	0.14379298504858157	1.1871155670105202
>100	0.03460795140427564	0.7125539158779935
>500	0.005575272761832823	0.47840398197148354
>1k	0.02508870809311993	9.180976835436748
>5k	0.005575268465136985	4.703300563603773
>10k+	0.0013938171162842462	16.837987331784113
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT	92390	16.896426860179737	Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	8147	1.4899360280320846	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	6542	1.1964111323660118	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	5971	1.091985764499764	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	5147	0.9412913632356869	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	4909	0.8977655531618393	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	4775	0.8732594248009335	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	4649	0.8502163488794847	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	4444	0.8127256301183975	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4350	0.7955347639547771	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	4079	0.7459738625681691	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	3371	0.6164937216762192	No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	2685	0.49103697499277615	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2663	0.4870135807842692	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	2650	0.48463612057015154	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2037	0.3725297273967542	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	2011	0.3677748069685188	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	1616	0.2955365927703264	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	1486	0.27176199062914913	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1379	0.25219366425141093	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1148	0.2099480250620883	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1078	0.197146316216839	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	1046	0.19129410645901077	No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	987	0.18050409471801493	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCC	600	0.1097289329592796	Illumina Single End PCR Primer 1 (96% over 32bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.006035091312760378	0.0	0.0
2	0.008046788417013838	0.0	0.0
3	0.010790011740995826	0.0	0.0
4	0.013898998174842081	0.0	0.0
5	0.016093576834027675	0.0	0.0
6	0.019568326377738195	0.0	0.0
7	0.024506128360905776	0.0	0.0
8	0.029809693453937622	0.0	0.0
9	0.03511325854696947	0.0	0.0
10-14	0.05855867388926887	0.0	0.0
15-19	0.08924619880688074	0.0	0.0
20-24	0.11817806079714413	0.0	0.0
25-29	0.14740253327529892	0.0	0.0
30-34	0.17348144300862106	0.0	0.0
35-39	0.19473227969173487	0.0	0.0
40-44	0.21539789539906584	0.0	0.0
45-49	0.2351856796427226	0.0	0.0
50-54	0.2572411951675378	0.0	0.0
55-59	0.283502986455792	0.0	0.0
60-64	0.31177647484829973	0.0	0.0
65-69	0.3484625147676855	0.0	0.0
70-74	0.3846364863332615	0.0	0.0
75-79	0.429881383023471	0.0	0.0
80-84	0.4794422844100789	0.0	0.0
85-89	0.5406344526903706	0.0	0.0
90-94	0.6182493846035677	0.0	0.0
95-99	0.6976931320660861	0.0	0.0
100-104	0.7929378458747408	0.0	0.0
105-109	0.895863584990545	0.0	0.0
110-114	1.0112984224637072	0.0	0.0
115-119	1.1427902604599107	0.0	0.0
120-124	1.278488374219553	0.0	0.0
125-129	1.4415455685970424	0.0	0.0
130-134	1.6323641830132296	0.0	0.0
135-139	1.8438484131367479	0.0	0.0
140-144	2.08005822948709	0.0	0.0
145-149	2.3600133137771993	0.0	0.0
150-154	2.6751913855472362	0.0	0.0
155-159	3.024897494888461	0.0	0.0
160-164	3.3964030855775946	0.0	0.0
165-169	3.801522306063255	0.0	0.0
170-174	4.277819027728501	0.0	0.0
175-179	4.852323144392303	0.0	0.0
180-184	5.497785304369772	0.0	0.0
185-189	6.206561058664745	0.0	7.315262197285307E-5
190-194	6.9903548267928795	0.0	1.8288155493213266E-4
195-199	7.839949378385595	0.0	1.8288155493213266E-4
200-204	8.775571413418387	0.0	1.8288155493213266E-4
205-209	9.78434607042403	0.0	1.8288155493213266E-4
210-214	10.879038481936789	0.0	2.1945786591855918E-4
215-219	12.047029820666348	0.0	3.657631098642653E-4
220-224	13.258620122091724	0.0	3.657631098642653E-4
225-229	14.48143935098994	0.0	3.657631098642653E-4
230-234	15.71808442544102	0.0	3.657631098642653E-4
235-239	17.006302098382964	0.0	3.657631098642653E-4
240-244	18.230438074476684	0.0	3.657631098642653E-4
245-249	19.415876313546768	0.0	3.657631098642653E-4
250-254	20.536281871682984	0.0	3.657631098642653E-4
255-259	21.640520700363204	0.0	5.48644664796398E-4
260-264	22.745271597397227	1.4630524394570614E-4	5.48644664796398E-4
265-269	23.754448593823724	1.8288155493213266E-4	5.48644664796398E-4
270-274	24.62880530795425	1.8288155493213266E-4	5.48644664796398E-4
275-279	25.404223100866496	1.8288155493213266E-4	5.48644664796398E-4
280-284	26.121228525133414	1.8288155493213266E-4	5.48644664796398E-4
285-288	26.753559789466753	1.8288155493213266E-4	5.48644664796398E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTTAT	4540	0.0	290.4383	2
CGGGTTA	4550	0.0	289.8	1
GGGGAGC	4340	0.0	270.62903	3
GTTATTA	4905	0.0	266.42816	4
GGGGGGA	4455	0.0	263.64313	1
GGGAGCG	3210	0.0	262.85983	4
GGTTATT	5010	0.0	262.6048	3
TTATTAG	5005	0.0	261.1049	5
TTAGTAG	5045	0.0	257.5778	8
GGAGCGA	3295	0.0	255.63278	5
TATTAGT	5255	0.0	248.1237	6
ATTAGTA	5530	0.0	235.519	7
GGGAGCA	1450	0.0	228.10345	4
GGGGGAG	5200	0.0	227.56732	2
TAGTAGA	5725	0.0	226.21309	9
GAGCGAA	3795	0.0	221.95258	6
AGCGAAA	3855	0.0	219.26068	7
CGAAAGC	3930	0.0	216.57253	9
GCGAAAG	4145	0.0	204.98433	8
GGAGCAA	2590	0.0	127.702705	5
>>END_MODULE