FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n02 clinical flu pool d1.3420000000b5e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n02 clinical flu pool d1.3420000000b5e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546802
Sequences flagged as poor quality0
Sequence length300
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT9239016.896426860179737Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC81471.4899360280320846No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA65421.1964111323660118No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT59711.091985764499764No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG51470.9412913632356869No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG49090.8977655531618393No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT47750.8732594248009335No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT46490.8502163488794847No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT44440.8127256301183975No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA43500.7955347639547771No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT40790.7459738625681691No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT33710.6164937216762192No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA26850.49103697499277615No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA26630.4870135807842692No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA26500.48463612057015154No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT20370.3725297273967542No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG20110.3677748069685188No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA16160.2955365927703264No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT14860.27176199062914913No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT13790.25219366425141093No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA11480.2099480250620883No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG10780.197146316216839No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA10460.19129410645901077No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA9870.18050409471801493No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCC6000.1097289329592796Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT45400.0290.43832
CGGGTTA45500.0289.81
GGGGAGC43400.0270.629033
GTTATTA49050.0266.428164
GGGGGGA44550.0263.643131
GGGAGCG32100.0262.859834
GGTTATT50100.0262.60483
TTATTAG50050.0261.10495
TTAGTAG50450.0257.57788
GGAGCGA32950.0255.632785
TATTAGT52550.0248.12376
ATTAGTA55300.0235.5197
GGGAGCA14500.0228.103454
GGGGGAG52000.0227.567322
TAGTAGA57250.0226.213099
GAGCGAA37950.0221.952586
AGCGAAA38550.0219.260687
CGAAAGC39300.0216.572539
GCGAAAG41450.0204.984338
GGAGCAA25900.0127.7027055
Produced by FastQC (version 0.11.2)