FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n02 clinical flu pool c4.3420000000b754.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n02 clinical flu pool c4.3420000000b754.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808888
Sequences flagged as poor quality0
Sequence length300
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT430765.3253355223467285Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC213982.6453600498462086No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA176582.1829969043922026No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG148141.8314031114319906No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTTAATTGTCGTACTCTTC132971.6438616965513149No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG121751.5051527529151132No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA81301.0050835220698044No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT70320.8693416146611151No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG65540.8102481431298276No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA63580.7860173472718103No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAACATTAATTG59180.7316216831007506No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG55710.6887232843113015No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT54500.6737644766642601No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT41910.518118701229342No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA32670.40388780647011696No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT31100.3844784444818071No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACA28400.3510992869222933No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT25740.3182146354006982No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT22080.27296733293113506No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA20830.257514019246175No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA14460.17876393270761837No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACA13860.1713463421388375No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA11580.1431594979774703No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT10780.13326937721909585No Hit
GGGGGGAGCGAAAGCAGGTACTGGGCCATAAGGACCAGGAGTGGAGGAAA9640.11917595513841223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT86400.0288.725682
CGGGTTA86350.0288.55241
GGGGGGA81200.0281.508641
GGTTATT89000.0280.291023
GGGGAGC85200.0270.535223
TTAGTAG93300.0266.112528
GTTATTA94150.0264.490724
GGGAGCG57850.0260.96634
TTATTAG95600.0260.325325
TATTAGT95550.0260.307686
GGAGCGA58000.0259.531045
TAGTAGA96150.0257.7669
ATTAGTA98850.0252.361167
CGAAAGC61400.0245.638439
GGGGGAG94450.0244.040242
GCGAAAG65500.0230.935878
AGCGAAA66100.0228.172457
GAGCGAA68900.0218.899866
GGGAGCA42350.0187.785134
CAAAAGC47500.0165.258949
Produced by FastQC (version 0.11.2)