##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE77A l01n02 clinical flu pool c4.3420000000b754.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	808888
Sequences flagged as poor quality	0
Sequence length	300
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.18134401796046	34.0	34.0	34.0	34.0	34.0
2	33.454709428252116	34.0	34.0	34.0	34.0	34.0
3	33.46945683456795	34.0	34.0	34.0	34.0	34.0
4	33.45323580025912	34.0	34.0	34.0	34.0	34.0
5	33.46042220925518	34.0	34.0	34.0	34.0	34.0
6	37.0031364045455	38.0	38.0	38.0	36.0	38.0
7	37.08710105725391	38.0	38.0	38.0	37.0	38.0
8	37.036255946435105	38.0	38.0	38.0	36.0	38.0
9	37.010509489570865	38.0	38.0	38.0	36.0	38.0
10-14	37.04186339765209	38.0	38.0	38.0	36.6	38.0
15-19	37.08120901781211	38.0	38.0	38.0	37.0	38.0
20-24	37.06964449960934	38.0	38.0	38.0	36.8	38.0
25-29	36.96669229856296	38.0	38.0	38.0	36.4	38.0
30-34	37.07288697569997	38.0	38.0	38.0	37.0	38.0
35-39	36.99455227423327	38.0	38.0	38.0	36.6	38.0
40-44	36.9322183046355	38.0	38.0	38.0	36.0	38.0
45-49	36.88672127661678	38.0	38.0	38.0	36.0	38.0
50-54	36.77697654063356	38.0	38.0	38.0	35.8	38.0
55-59	36.74153034783554	38.0	38.0	38.0	35.4	38.0
60-64	36.64422194419994	38.0	38.0	38.0	34.6	38.0
65-69	36.36219921670243	38.0	37.4	38.0	34.0	38.0
70-74	36.65781764595346	38.0	38.0	38.0	34.8	38.0
75-79	36.55417437271909	38.0	37.8	38.0	34.6	38.0
80-84	35.48734138718834	38.0	37.0	38.0	29.4	38.0
85-89	35.493838949273574	38.0	37.0	38.0	30.2	38.0
90-94	35.471117385843286	38.0	37.0	38.0	29.4	38.0
95-99	35.306614760016224	38.0	37.0	38.0	27.2	38.0
100-104	35.36482479651077	38.0	37.0	38.0	27.6	38.0
105-109	35.16785290423396	38.0	37.0	38.0	26.6	38.0
110-114	35.033404871873486	38.0	37.0	38.0	25.8	38.0
115-119	35.080169566120404	38.0	37.0	38.0	26.8	38.0
120-124	34.816032133002345	38.0	37.0	38.0	24.2	38.0
125-129	34.74790329439923	38.0	36.6	38.0	24.0	38.0
130-134	34.65682072178102	38.0	36.2	38.0	24.0	38.0
135-139	34.30423074640741	38.0	35.8	38.0	23.2	38.0
140-144	34.15365069082493	38.0	35.2	38.0	23.0	38.0
145-149	33.85559978637339	38.0	34.8	38.0	21.4	38.0
150-154	33.51915765841501	38.0	34.0	38.0	20.0	38.0
155-159	33.223879696571096	37.2	34.0	38.0	18.8	38.0
160-164	32.80721335957512	37.0	32.2	38.0	17.2	38.0
165-169	31.96423460355451	36.8	29.2	38.0	12.6	38.0
170-174	31.123355272917884	36.4	26.4	38.0	11.0	38.0
175-179	31.22964242268398	36.6	27.4	38.0	11.0	38.0
180-184	31.097102689123837	37.0	27.2	38.0	11.0	38.0
185-189	30.531556655556766	35.6	24.4	38.0	10.4	38.0
190-194	29.63157890832847	35.0	22.4	37.8	10.0	38.0
195-199	28.959266548644557	34.6	21.2	37.2	10.0	38.0
200-204	28.152935635094103	33.8	19.6	37.0	10.0	37.8
205-209	27.14827738821691	32.0	18.0	37.0	9.4	37.2
210-214	26.37101823738268	30.4	16.4	37.0	9.0	37.0
215-219	25.270208483745584	28.4	14.8	36.6	9.0	37.0
220-224	24.076936980150528	26.0	12.8	35.6	8.4	37.0
225-229	23.045951973573597	24.0	10.6	34.0	8.2	37.0
230-234	21.975691072187992	22.2	9.8	32.4	8.0	37.0
235-239	20.85063717102986	20.4	9.0	31.4	8.0	36.4
240-244	19.970883484487345	18.8	9.0	29.8	7.8	36.0
245-249	19.116360237758506	17.4	8.4	28.6	7.2	35.2
250-254	18.05207000227473	15.2	8.0	26.4	7.0	33.8
255-259	17.14053218739801	13.8	8.0	24.6	7.0	32.8
260-264	16.603793603069896	13.4	8.0	23.6	7.0	31.6
265-269	16.090318560789626	13.0	8.0	22.2	7.0	31.2
270-274	15.209898774614038	12.4	8.0	21.0	7.0	29.4
275-279	14.44738183778224	12.0	8.0	19.2	7.0	27.2
280-284	13.841043506641217	11.2	8.0	18.2	7.0	26.4
285-289	13.565442434552125	11.0	8.0	17.8	7.0	25.6
290-294	13.051780716242543	11.0	8.0	16.8	7.0	24.2
295-299	12.58348695987578	9.0	8.0	15.6	7.0	22.8
300	11.71336946524117	9.0	8.0	13.0	7.0	20.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.10151947509820758
1101	2	0.04952218751091664
1101	3	0.07467480914502289
1101	4	0.07743287952997235
1101	5	0.09280194756654225
1101	6	0.22017306655678937
1101	7	0.11697881383184239
1101	8	0.22067944287827856
1101	9	0.2469324631694505
1101	10-14	0.13331138957954636
1101	15-19	0.1345177421378949
1101	20-24	0.12291062010455533
1101	25-29	0.1372391562454709
1101	30-34	0.14238152513283353
1101	35-39	0.1259467364954574
1101	40-44	0.11217845020798478
1101	45-49	0.12269171522794409
1101	50-54	0.21234421849211316
1101	55-59	0.2044051559788045
1101	60-64	0.22882234112420008
1101	65-69	0.2679903196405178
1101	70-74	0.1799898952633754
1101	75-79	0.2453933805774824
1101	80-84	0.3995855258493748
1101	85-89	0.3742950218040164
1101	90-94	0.3679825290745029
1101	95-99	0.36329426704685375
1101	100-104	0.5233276680015422
1101	105-109	0.4916065699663221
1101	110-114	0.4755338817512822
1101	115-119	0.4994211808651272
1101	120-124	0.38296814366846377
1101	125-129	0.3928958225144683
1101	130-134	0.44321733923104034
1101	135-139	0.3798286763955119
1101	140-144	0.33518269990322835
1101	145-149	0.4752332544343716
1101	150-154	0.4654154103852264
1101	155-159	0.4316345733222775
1101	160-164	0.34611436646155624
1101	165-169	0.7320420893391031
1101	170-174	1.4065390562791578
1101	175-179	0.6835389736688064
1101	180-184	0.10270620117123741
1101	185-189	0.29725126050773554
1101	190-194	-0.12292253903727257
1101	195-199	-0.26584217376069574
1101	200-204	-0.16849286826473886
1101	205-209	0.05341243581264621
1101	210-214	-0.7515338341983515
1101	215-219	-0.3258546818405108
1101	220-224	-0.561920279300697
1101	225-229	-0.6103789049668258
1101	230-234	-0.29603839183212344
1101	235-239	-0.3391516158750072
1101	240-244	-0.37229726388854445
1101	245-249	-0.5997610422194661
1101	250-254	-0.14154767039246607
1101	255-259	-0.11149261241309105
1101	260-264	-0.27437113569394356
1101	265-269	-0.4225463848374531
1101	270-274	-0.20538109801131732
1101	275-279	-0.010404637590164967
1101	280-284	0.018568981875628054
1101	285-289	-0.18111925328715905
1101	290-294	-0.20916215776411562
1101	295-299	-0.250745481564163
1101	300	0.7775796984034109
1102	1	-0.06883043282076073
1102	2	-0.0978074625811658
1102	3	-0.09475428846087652
1102	4	-0.12790782028586278
1102	5	-0.04439878908171124
1102	6	0.07422463193432094
1102	7	0.0925773405353425
1102	8	-0.003537868355607543
1102	9	0.11940023480849504
1102	10-14	0.01194859031619444
1102	15-19	-0.09982848253982723
1102	20-24	-0.11106728044792646
1102	25-29	-0.11285292478584097
1102	30-34	-0.0687602796553719
1102	35-39	-0.03593171654322447
1102	40-44	8.524833571641466E-4
1102	45-49	-0.09996021847371139
1102	50-54	-0.12255449237344607
1102	55-59	0.048419888943811884
1102	60-64	0.0020267610137025827
1102	65-69	0.19358884634401363
1102	70-74	-0.037137176559831175
1102	75-79	0.11694034190344382
1102	80-84	0.1808930764939447
1102	85-89	0.09989354850751653
1102	90-94	0.13179468377064296
1102	95-99	0.11366259117208699
1102	100-104	0.052333192863422084
1102	105-109	0.22650528083188703
1102	110-114	0.09477881729456072
1102	115-119	0.1124966873107951
1102	120-124	0.03324438676239083
1102	125-129	0.2034851411148324
1102	130-134	0.07268326924945256
1102	135-139	0.16748981820029485
1102	140-144	0.23905010321814757
1102	145-149	0.15202883454487193
1102	150-154	0.14497342143494762
1102	155-159	0.17693844072559273
1102	160-164	0.21572762613006802
1102	165-169	0.5537732127276911
1102	170-174	1.3607747837193216
1102	175-179	0.59302331989349
1102	180-184	0.40537655107915427
1102	185-189	0.2767171905261492
1102	190-194	0.06952681639550562
1102	195-199	-0.07330073729660924
1102	200-204	0.15369866028038714
1102	205-209	-0.09888958997004238
1102	210-214	0.1687239926893085
1102	215-219	0.1796701800379381
1102	220-224	-0.26274900858246397
1102	225-229	-0.06544336168873954
1102	230-234	-0.292815555736361
1102	235-239	-0.07377408364665072
1102	240-244	0.18985743224405383
1102	245-249	0.19535866312123318
1102	250-254	0.5919698250034813
1102	255-259	0.6627246988207993
1102	260-264	0.42848337627659205
1102	265-269	0.22128418606493838
1102	270-274	0.2602726773109634
1102	275-279	0.3755990456510876
1102	280-284	0.5045726651168785
1102	285-289	0.41225091245869727
1102	290-294	0.368554232659454
1102	295-299	0.29409798068261317
1102	300	1.0832887223444807
1103	1	-0.004558717748196273
1103	2	-0.011527536245985459
1103	3	0.11308865002224167
1103	4	0.045697025372810174
1103	5	0.05407215893052353
1103	6	0.1592449911348197
1103	7	0.020754980895560493
1103	8	-0.07715200659541921
1103	9	0.03353509550425571
1103	10-14	0.00687974073773745
1103	15-19	-0.07755230702831994
1103	20-24	-0.1403367146142216
1103	25-29	-0.07485741901599141
1103	30-34	-0.0955600836663919
1103	35-39	-0.09348303693452209
1103	40-44	-0.10493493617753558
1103	45-49	-0.14505153920293168
1103	50-54	-0.27899881291680373
1103	55-59	-0.1330101548157927
1103	60-64	-0.32448463506990066
1103	65-69	-0.02863664236127761
1103	70-74	-0.16602088413075222
1103	75-79	-0.029615320657597977
1103	80-84	0.18043131968924087
1103	85-89	0.11835402124646777
1103	90-94	-0.005160052911406865
1103	95-99	-0.05470174559105345
1103	100-104	-0.12409304938572063
1103	105-109	-0.040678053346219656
1103	110-114	-0.07280375996057842
1103	115-119	-0.043496689613185424
1103	120-124	-0.04533266563087324
1103	125-129	0.07100368403811075
1103	130-134	0.012178428659453289
1103	135-139	-0.15186476980044006
1103	140-144	-0.04821061281747774
1103	145-149	0.1120340721815154
1103	150-154	0.017760013085137416
1103	155-159	0.02965069172665835
1103	160-164	0.1318933381952192
1103	165-169	0.45227913463622116
1103	170-174	1.2936850511766949
1103	175-179	0.6169855075360431
1103	180-184	0.4573241071303116
1103	185-189	0.29285401111174636
1103	190-194	0.03775750894623897
1103	195-199	0.04965751367066673
1103	200-204	0.27713894277485096
1103	205-209	0.509479308605659
1103	210-214	0.4927829921317546
1103	215-219	0.3655785720834892
1103	220-224	0.39048886459914556
1103	225-229	0.2732109036060031
1103	230-234	0.3634567675778868
1103	235-239	0.1871698736412739
1103	240-244	0.34233263794800806
1103	245-249	0.413599324077186
1103	250-254	0.5995848418146075
1103	255-259	0.8721661302162076
1103	260-264	0.47497230120943
1103	265-269	0.26792246812012266
1103	270-274	0.45695133985510417
1103	275-279	0.5629270061829583
1103	280-284	0.8055201704812323
1103	285-289	0.5283083237145458
1103	290-294	0.33458984858862806
1103	295-299	0.2988495308541044
1103	300	1.2441475257979402
2116	1	0.127513218353819
2116	2	0.0024281014619589314
2116	3	0.06462759421325615
2116	4	0.07566955331122216
2116	5	0.1442949878745452
2116	6	0.23303036989315018
2116	7	0.12703794312994887
2116	8	0.05721944281020086
2116	9	0.0326100331833743
2116	10-14	0.1974615718693542
2116	15-19	0.09991178427389258
2116	20-24	-0.004317111093719461
2116	25-29	-0.006027694923673721
2116	30-34	0.09280329370137963
2116	35-39	0.05402294078258052
2116	40-44	0.08320764721373308
2116	45-49	-0.010912584837072359
2116	50-54	0.10556409301681668
2116	55-59	0.11316919365543754
2116	60-64	-0.036515363079530516
2116	65-69	0.18954613163862177
2116	70-74	0.05804141979165678
2116	75-79	0.09646499272740527
2116	80-84	0.31749503113084643
2116	85-89	0.2790784696854729
2116	90-94	0.2159727456776821
2116	95-99	0.26395568458412555
2116	100-104	0.40474040548039625
2116	105-109	0.35351991507785385
2116	110-114	0.32792577589724914
2116	115-119	0.23406117977582142
2116	120-124	0.4394818979533852
2116	125-129	0.47594797680210377
2116	130-134	0.4085892546866958
2116	135-139	0.42035164899587585
2116	140-144	0.5342572940488495
2116	145-149	0.52771671899594
2116	150-154	0.6222031950960272
2116	155-159	0.7227273930443019
2116	160-164	0.8170948847705333
2116	165-169	1.173208097311516
2116	170-174	1.9571960484803554
2116	175-179	1.441342373007732
2116	180-184	1.0026752238187413
2116	185-189	1.1284832400286362
2116	190-194	1.1511613312935722
2116	195-199	0.8866581411189891
2116	200-204	1.0753715734791207
2116	205-209	1.0470234419638622
2116	210-214	1.0257255551733522
2116	215-219	1.3048641289014817
2116	220-224	1.6571869602087084
2116	225-229	0.8586479976723744
2116	230-234	0.9380191647696279
2116	235-239	0.9356261811926956
2116	240-244	1.0510422989563324
2116	245-249	0.882343579533444
2116	250-254	1.1967753995652899
2116	255-259	1.4357458076738503
2116	260-264	1.0549409287934175
2116	265-269	0.9524896135694263
2116	270-274	0.9729531374097515
2116	275-279	0.9629488820838557
2116	280-284	1.0459471745002933
2116	285-289	0.7944111913229506
2116	290-294	0.6642299984979037
2116	295-299	0.5667604671087485
2116	300	1.0788191329135444
1117	1	0.038236503424961654
1117	2	-0.18706235645536395
1117	3	-0.05700735244985822
1117	4	-0.027744865779844474
1117	5	-0.11695985720490398
1117	6	-0.07691273854660352
1117	7	-0.06168521069180599
1117	8	-0.024701417176153484
1117	9	-0.12534544635224165
1117	10-14	0.015131777501800059
1117	15-19	-0.13100442897943765
1117	20-24	0.015117052616325566
1117	25-29	0.0076952695960912365
1117	30-34	0.05446518014205992
1117	35-39	-0.021613723901317883
1117	40-44	0.010512567298839315
1117	45-49	0.14906817366657776
1117	50-54	0.11704408127167909
1117	55-59	0.12891958256587088
1117	60-64	0.0988706910002577
1117	65-69	0.3432879169836198
1117	70-74	0.22043211892178505
1117	75-79	0.23631428309731461
1117	80-84	0.202321971244956
1117	85-89	0.1374502032795064
1117	90-94	0.0976098209365901
1117	95-99	0.14591928475475413
1117	100-104	0.32094776941222136
1117	105-109	0.39119054979484247
1117	110-114	0.13413643789054674
1117	115-119	0.17412396073152792
1117	120-124	0.256841942780099
1117	125-129	0.2231574550269002
1117	130-134	0.23170046245867582
1117	135-139	0.13687847056485225
1117	140-144	0.3377011601172697
1117	145-149	0.4730168552092522
1117	150-154	0.48032101161106766
1117	155-159	0.5938488685566128
1117	160-164	0.7608932672060007
1117	165-169	1.058710214549194
1117	170-174	1.448622392755908
1117	175-179	1.035316175293996
1117	180-184	0.7110855750202241
1117	185-189	0.8665929712450442
1117	190-194	0.9264893075206082
1117	195-199	1.069110771449207
1117	200-204	0.9406749572733553
1117	205-209	0.6571814712711763
1117	210-214	0.37728199619484215
1117	215-219	0.5621922195510543
1117	220-224	0.9842960578944258
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2108	175-179	0.0351662610008141
2108	180-184	-0.14697589070081563
2108	185-189	-0.417784069873786
2108	190-194	0.05651583197744614
2108	195-199	0.07068317137990121
2108	200-204	0.17772410337953204
2108	205-209	-0.19433399523106942
2108	210-214	0.06716540694946005
2108	215-219	-0.3159903870767238
2108	220-224	-0.3936200559552212
2108	225-229	-0.3109622154431655
2108	230-234	-0.4169880672628139
2108	235-239	-0.4597963137055565
2108	240-244	-0.5950107155557944
2108	245-249	0.4357378279919928
2108	250-254	-0.09198064715669929
2108	255-259	0.5241554160079538
2108	260-264	-0.00668883484366134
2108	265-269	-0.48451208628593356
2108	270-274	-0.14190761865444834
2108	275-279	-0.06649152028807492
2108	280-284	0.10924541827440848
2108	285-289	-0.18180901204791944
2108	290-294	-0.15460094673529312
2108	295-299	1.1658575773546342
2108	300	1.459688549937443
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	111.0
11	346.0
12	1040.0
13	1600.0
14	1596.0
15	1490.0
16	1917.0
17	3943.0
18	18366.0
19	22189.0
20	9998.0
21	6514.0
22	8590.0
23	14615.0
24	22884.0
25	31891.0
26	40587.0
27	48653.0
28	57242.0
29	66955.0
30	76946.0
31	87971.0
32	97781.0
33	92865.0
34	65110.0
35	24760.0
36	2906.0
37	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.39240043120926	23.576935249379392	14.79636241358507	27.234301905826268
2	37.5468544470928	29.165347983898883	19.126257281601408	14.16154028740691
3	37.543022025298924	21.614364411389463	25.528626954535117	15.313986608776492
4	37.37983503278575	20.863333366300402	20.032934102125388	21.72389749878846
5	32.63294794829445	20.983003827476736	30.70956671380958	15.674481510419241
6	32.123112223200245	20.535475863160286	30.864099850659176	16.47731206298029
7	16.78254591488562	46.43337520151121	20.53893740542572	16.245141478177448
8	25.862047650601816	26.159369405900446	31.385185588116034	16.593397355381708
9	21.44054553906103	20.26794809664626	31.968084580312727	26.323421783979985
10-14	25.964212598035825	32.78241239825538	24.075372610299574	17.178002393409223
15-19	23.571100078131956	30.7659898527361	24.17966393369663	21.483246135435312
20-24	26.4851499836813	30.674926565853365	25.573627003985717	17.26629644647961
25-29	21.23520190681528	28.557229183768335	32.682126573765466	17.52544233565092
30-34	19.660323802553627	28.15494852192145	32.640860044901146	19.543867630623772
35-39	21.11985837347074	30.037038502240108	28.774391510320342	20.068711613968805
40-44	20.720297494832412	29.340032241793672	29.060636330369594	20.879033933004322
45-49	21.505202203518905	29.41376309204735	27.006532424760906	22.074502279672835
50-54	23.148297415711447	28.010701110660563	28.0080555033577	20.8329459702703
55-59	24.02332584980863	25.233307948690054	28.379627340249826	22.363738861251495
60-64	22.044337411359795	26.07033359377318	28.696729337065207	23.188599657801824
65-69	19.100493517025843	26.29466625787501	30.971061506660995	23.633778718438155
70-74	20.07380502615937	29.815994303290445	28.601734727181018	21.508465943369167
75-79	19.34527400579561	29.46665051280276	29.98279118988043	21.2052842915212
80-84	21.425067500074178	29.99641483122509	26.026545084115476	22.551972584585258
85-89	21.25955632918278	28.526915963643916	26.072979201076045	24.14054850609726
90-94	19.88203558465449	31.263067322052002	27.77178051844013	21.083116574853378
95-99	23.33687729327175	28.625100137472682	27.105705610665503	20.932316958590064
100-104	22.850159725450247	28.06541820375627	27.075219313427816	22.009202757365667
105-109	23.746674446894996	28.95466368644361	24.400287802514068	22.898374064147323
110-114	21.59431219155186	28.970018098920985	28.82203716707381	20.613632542453345
115-119	23.70048758295339	28.64448477415909	27.23284311301441	20.42218452987311
120-124	20.802954179070525	28.698979339537733	27.31478276349754	23.183283717894195
125-129	21.324484971961507	26.039946197743074	30.736443116970456	21.89912571332496
130-134	25.06960172483706	28.706668908427375	26.888518558811604	19.335210807923964
135-139	23.84023498926922	28.11835507511547	26.41675979863714	21.624650136978172
140-144	22.429384537785207	28.770657989734055	27.32309046493458	21.47686700754616
145-149	22.826695413951004	27.997893404278468	28.410212538695095	20.765198643075433
150-154	23.857968929683448	27.51222110161632	25.75741654157721	22.87239342712302
155-159	22.617000128571572	27.455123577058878	29.33496355490501	20.59291273946455
160-164	24.545919830681132	30.663256223358488	26.044915983424154	18.745907962536222
165-169	22.558110037016256	28.35802443799993	27.377888503201557	21.705977021782257
170-174	23.042497848898734	30.62095123181454	25.9010641769936	20.435486742293126
175-179	21.118251228847505	30.73327827832778	27.54858521822551	20.599885274599202
180-184	21.175885907566933	31.31016902216376	26.902265826665744	20.611679243603565
185-189	22.193832074114606	30.064738273960863	27.60296298201061	20.138466669913925
190-194	20.738099712197485	30.53901158133141	27.019142328727835	21.703746377743272
195-199	21.62685068884691	32.492137353997094	26.116050676978762	19.764961280177232
200-204	22.525664863367982	30.380720198593625	26.885032291244276	20.208582646794117
205-209	21.3094078066204	30.811022245606868	26.971676417916058	20.907893529856675
210-214	23.731010340620873	29.735453974075014	27.686714445863675	18.846821239440438
215-219	22.45940660744296	31.06161324228156	27.279555953248398	19.199424197027078
220-224	20.127483656575446	31.1383528992889	27.443552135771576	21.290611308364074
225-229	21.746298622306178	32.17105458357646	25.953209838692132	20.129436955425227
230-234	21.1906716381007	32.11072484695038	26.040242901365822	20.658360613583092
235-239	21.59216109028691	30.883632839156967	25.78742668947988	21.736779381076243
240-244	21.839691487496783	34.3082439863724	23.81541667459937	20.03664785153145
245-249	20.71100078131954	32.821008594514936	25.010261000286814	21.457729623878706
250-254	21.013742573444674	31.30671522057818	25.62318284439449	22.056359361582658
255-259	21.479858769075573	31.73435630149044	25.760698638130368	21.025086291303616
260-264	19.971664804027256	32.67433810366824	26.134149597966594	21.219847494337905
265-269	20.04067807673697	33.194764465479636	25.57571025301655	21.18884720476684
270-274	19.488235701357915	33.9784741521694	24.55548852251486	21.97780162395783
275-279	20.07672261178309	32.913505948907634	24.1457655447973	22.864005894511973
280-284	18.06742095320984	33.71124308927812	25.24809367922382	22.973242278288218
285-289	19.669793593179776	32.8394783950312	25.700195824391013	21.790532187398007
290-294	19.301881100968245	34.54302697035931	24.45715599687472	21.697935931797726
295-299	18.55980061516551	35.29210471659859	23.393918564745675	22.754176103490224
300	9.83164541939057	50.17777492063178	19.93934883444927	20.05123082552838
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.25
6	0.25
7	0.0
8	0.25
9	0.0
10	0.0
11	1.5
12	1.75
13	1.0
14	1.5
15	3.0
16	5.0
17	3.5
18	8.75
19	10.75
20	13.75
21	19.25
22	23.0
23	30.75
24	34.75
25	42.25
26	53.5
27	57.75
28	66.75
29	83.25
30	106.5
31	174.75
32	305.0
33	621.0
34	1306.75
35	2414.25
36	4224.25
37	8096.25
38	12308.25
39	16182.75
40	20154.25
41	22868.25
42	25937.0
43	29134.5
44	28480.25
45	26550.0
46	21774.0
47	15876.75
48	10164.0
49	7651.5
50	5971.0
51	5206.75
52	2522.25
53	679.25
54	174.25
55	64.5
56	32.5
57	22.5
58	15.75
59	12.75
60	11.0
61	10.75
62	5.5
63	6.75
64	6.25
65	4.75
66	4.25
67	4.0
68	4.75
69	3.75
70	2.5
71	2.75
72	2.0
73	2.25
74	1.75
75	2.25
76	1.25
77	1.0
78	1.0
79	1.75
80	1.5
81	1.75
82	2.0
83	3.0
84	1.75
85	1.25
86	1.0
87	0.75
88	1.75
89	0.25
90	0.5
91	0.75
92	1.0
93	0.75
94	0.25
95	0.25
96	0.75
97	0.25
98	0.0
99	0.0
100	0.3333333333333333
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	2.4725301895936152E-5
155-159	0.0
160-164	0.0
165-169	2.4725301895936152E-5
170-174	0.0
175-179	0.0
180-184	0.0
185-189	2.4725301895936152E-5
190-194	0.0
195-199	0.0
200-204	0.0
205-209	2.4725301895936152E-5
210-214	4.9450603791872304E-5
215-219	2.4725301895936152E-5
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	2.4725301895936152E-5
245-249	0.0
250-254	2.4725301895936152E-5
255-259	0.0
260-264	0.0
265-269	2.4725301895936152E-5
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	808888.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.810053723360431
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.87157585094593	6.363936442168307
2	2.4228662960919416	0.47536897058362493
3	1.705145872682467	0.5018271785154392
4	1.3665471575221195	0.5362360412318993
5	1.2420934758887385	0.6092501863952009
6	1.2915643004699815	0.7602189104870971
7	1.3981162484485188	0.9600916856148176
8	1.4224696181789402	1.1163602699346722
9	1.4264005462793312	1.2593759391027814
>10	20.18713756296858	43.594672136153406
>50	2.2420598213145357	14.895860057055089
>100	0.39019175628385333	4.979116174105087
>500	0.003759296313537464	0.25200203600892
>1k	0.013783090124829518	3.114935405163032
>5k	0.008771057338971275	5.533032922624874
>10k+	0.007518049147689664	15.04771564485575
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT	43076	5.3253355223467285	Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	21398	2.6453600498462086	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	17658	2.1829969043922026	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	14814	1.8314031114319906	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTTAATTGTCGTACTCTTC	13297	1.6438616965513149	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	12175	1.5051527529151132	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	8130	1.0050835220698044	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	7032	0.8693416146611151	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	6554	0.8102481431298276	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	6358	0.7860173472718103	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAACATTAATTG	5918	0.7316216831007506	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	5571	0.6887232843113015	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT	5450	0.6737644766642601	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	4191	0.518118701229342	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	3267	0.40388780647011696	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3110	0.3844784444818071	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACA	2840	0.3510992869222933	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT	2574	0.3182146354006982	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2208	0.27296733293113506	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA	2083	0.257514019246175	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	1446	0.17876393270761837	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACA	1386	0.1713463421388375	No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	1158	0.1431594979774703	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1078	0.13326937721909585	No Hit
GGGGGGAGCGAAAGCAGGTACTGGGCCATAAGGACCAGGAGTGGAGGAAA	964	0.11917595513841223	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0038324217938701033	0.0	0.0
2	0.003956048303349784	0.0	0.0
3	0.0040796748128294645	0.0	0.0
4	0.0040796748128294645	0.0	0.0
5	0.004697807360227868	0.0	0.0
6	0.004821433869707549	0.0	0.0
7	0.005439566417105953	0.0	0.0
8	0.005810445945544995	0.0	0.0
9	0.005934072455024676	0.0	0.0
10-14	0.0082088002294508	0.0	0.0
15-19	0.010162099079229757	0.0	0.0
20-24	0.011793969004361544	0.0	0.0
25-29	0.013153860608638032	0.0	0.0
30-34	0.05607698469998319	0.0	0.0
35-39	0.061269298098129775	0.0	0.0
40-44	0.07387920206505721	0.0	0.0
45-49	0.10325286071742937	0.0	0.0
50-54	0.16593150102362747	0.0	0.0
55-59	0.2813492102738574	0.0	0.0
60-64	0.4722532662123804	0.0	0.0
65-69	0.7507837920701012	0.0	0.0
70-74	1.1269545351148738	0.0	0.0
75-79	1.6039797845931698	0.0	0.0
80-84	2.179832065749523	0.0	0.0
85-89	2.856637754546983	0.0	0.0
90-94	3.612910563637982	0.0	0.0
95-99	4.482029650582033	0.0	0.0
100-104	5.423173541948947	0.0	0.0
105-109	6.450015329687176	0.0	0.0
110-114	7.544876422941124	0.0	0.0
115-119	8.70241615650127	0.0	0.0
120-124	9.937469711505178	0.0	0.0
125-129	11.233199157361712	0.0	0.0
130-134	12.596873732828277	0.0	0.0
135-139	13.984185696907357	0.0	0.0
140-144	15.407126821018485	0.0	0.0
145-149	16.884513059904464	0.0	0.0
150-154	18.38936910919682	0.0	0.0
155-159	19.88799438241141	0.0	0.0
160-164	21.28284756356875	0.0	0.0
165-169	22.653222695849117	0.0	0.0
170-174	24.13214190345264	0.0	1.2362650947968075E-4
175-179	25.654874346015767	0.0	1.2362650947968075E-4
180-184	27.159557318194857	0.0	1.978024151674892E-4
185-189	28.657490282956353	0.0	2.472530189593615E-4
190-194	30.190879330636626	0.0	2.472530189593615E-4
195-199	31.728199701318356	0.0	2.472530189593615E-4
200-204	33.201407364183915	0.0	2.472530189593615E-4
205-209	34.643609498471974	0.0	2.7197832085529766E-4
210-214	36.06148193569443	0.0	3.7087952843904226E-4
215-219	37.437692239222244	0.0	3.7087952843904226E-4
220-224	38.761880507561	0.0	3.7087952843904226E-4
225-229	40.061936881249316	0.0	3.7087952843904226E-4
230-234	41.31758661273254	0.0	3.7087952843904226E-4
235-239	42.5285824489917	0.0	3.7087952843904226E-4
240-244	43.63929246076095	0.0	3.7087952843904226E-4
245-249	44.63619190790319	0.0	3.7087952843904226E-4
250-254	45.56957205447478	0.0	3.7087952843904226E-4
255-259	46.47033952784564	0.0	3.7087952843904226E-4
260-264	47.330236077182505	0.0	3.7087952843904226E-4
265-269	48.11889903175718	0.0	3.7087952843904226E-4
270-274	48.78702613958917	0.0	4.4505543412685065E-4
275-279	49.353309728911796	0.0	4.94506037918723E-4
280-284	49.8655685335918	0.0	4.94506037918723E-4
285-288	50.32238703009564	0.0	4.94506037918723E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTTAT	8640	0.0	288.72568	2
CGGGTTA	8635	0.0	288.5524	1
GGGGGGA	8120	0.0	281.50864	1
GGTTATT	8900	0.0	280.29102	3
GGGGAGC	8520	0.0	270.53522	3
TTAGTAG	9330	0.0	266.11252	8
GTTATTA	9415	0.0	264.49072	4
GGGAGCG	5785	0.0	260.9663	4
TTATTAG	9560	0.0	260.32532	5
TATTAGT	9555	0.0	260.30768	6
GGAGCGA	5800	0.0	259.53104	5
TAGTAGA	9615	0.0	257.766	9
ATTAGTA	9885	0.0	252.36116	7
CGAAAGC	6140	0.0	245.63843	9
GGGGGAG	9445	0.0	244.04024	2
GCGAAAG	6550	0.0	230.93587	8
AGCGAAA	6610	0.0	228.17245	7
GAGCGAA	6890	0.0	218.89986	6
GGGAGCA	4235	0.0	187.78513	4
CAAAAGC	4750	0.0	165.25894	9
>>END_MODULE