FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n02 clinical flu pool c3.3420000000b6e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n02 clinical flu pool c3.3420000000b6e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814706
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT289223.549992267149131Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA219462.693732462998922No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC189792.329552010172995No Hit
GGGGGGAGCGAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACA179932.2085267568914433No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA164292.016555665479326No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAACATTAATTG112771.3841803055335298No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG111041.3629456515601945No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG102261.255176714053904No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA94501.1599276303353603No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTTAATTGTCGTACTCTTC92611.1367290777286532No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG88651.0881225865526951No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA87691.076339194752463No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG74010.9084258615991535No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT57090.7007435811200605No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT56100.688591958326071No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT54010.6629385324276488No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT44740.5491551553566563No Hit
GGGGGGAGCAAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACA44540.5467002820649413No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT43200.5302526310104504No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA42110.5168735715706034No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT41920.5145414419434741No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA34920.42862087673344745No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA22050.27064978041158405No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT13150.1614079189302644No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA8280.101631754277003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA105200.0289.10931
GGGTTAT105750.0287.883672
GGGGGGA111300.0285.41511
GGTTATT110550.0274.985083
TTAGTAG111550.0272.12468
GGGGAGC118050.0270.09153
TAGTAGA113950.0266.393169
TATTAGT114800.0264.03666
ATTAGTA115350.0263.66977
GTTATTA115350.0262.65024
GGGAGCG80300.0260.86554
GGGGGAG122700.0260.694372
TTATTAG116900.0258.664675
GGAGCGA80950.0258.226075
CGAAAGC86300.0242.89929
AGCGAAA89800.0234.086877
GCGAAAG89800.0233.595788
GAGCGAA92200.0226.718026
GGGAGCA51050.0215.388844
CAAAAGC63400.0171.577299
Produced by FastQC (version 0.11.2)