FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n02 clinical flu pool c2.3420000000b67e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n02 clinical flu pool c2.3420000000b67e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147070
Sequences flagged as poor quality0
Sequence length300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT416173.628113367100526Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC291942.5450931503744325No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA285112.4855501407935No Hit
GGGGGGAGCGAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACA254212.2161681501564856No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG211931.8475768697638328No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG151421.3200589327591166No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAACATTAATTG148661.2959976287410533No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA141721.2354956541449085No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTTAATTGTCGTACTCTTC141461.2332290095634966No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA129171.126086463772917No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG126841.1057738411779576No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT110780.9657649489569076No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG89600.7811205942095949No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA83330.7264595883424725No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT79130.6898445604888978No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT78050.6804292676122643No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT67230.5861019815704359No Hit
GGGGGGAGCAAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACA65020.566835502628436No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT59920.5223743973776666No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT55510.48392861813141314No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA54530.4753851116322456No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA47880.4174113175307523No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA30570.2665050955913763No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT21680.18900328663464305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT134900.0290.077122
CGGGTTA135100.0289.430051
GGGGGGA169200.0287.22341
GGTTATT141600.0276.24793
GGGGAGC178650.0273.346773
TTAGTAG143550.0272.188088
TAGTAGA145150.0269.18779
GGGAGCG121950.0267.239844
GTTATTA146800.0265.56134
TTATTAG147150.0264.929665
TATTAGT147900.0264.281926
GGAGCGA123500.0263.885835
ATTAGTA151200.0258.1257
GGGGGAG190500.0256.883452
CGAAAGC130700.0249.123939
GCGAAAG135700.0239.944738
AGCGAAA137550.0236.50387
GGGAGCA69350.0234.012974
GAGCGAA142950.0228.083956
CAAAAGC91550.0176.945929
Produced by FastQC (version 0.11.2)