##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE77A l01n02 clinical flu pool c1.3420000000b5cf.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	658553
Sequences flagged as poor quality	0
Sequence length	300
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.972822232986566	34.0	34.0	34.0	33.0	34.0
2	33.33304684664712	34.0	34.0	34.0	34.0	34.0
3	33.31809588598033	34.0	34.0	34.0	34.0	34.0
4	33.329548267185785	34.0	34.0	34.0	34.0	34.0
5	33.356477003369505	34.0	34.0	34.0	34.0	34.0
6	36.744757065870175	38.0	38.0	38.0	35.0	38.0
7	36.87123435775101	38.0	38.0	38.0	36.0	38.0
8	36.87317649452664	38.0	38.0	38.0	36.0	38.0
9	36.79203647997959	38.0	38.0	38.0	36.0	38.0
10-14	36.83514614617199	38.0	38.0	38.0	36.0	38.0
15-19	36.85261505148409	38.0	38.0	38.0	36.0	38.0
20-24	36.83474314140244	38.0	38.0	38.0	35.8	38.0
25-29	36.72765259591863	38.0	38.0	38.0	35.4	38.0
30-34	36.65464161578491	38.0	38.0	38.0	34.8	38.0
35-39	36.516370284548096	38.0	37.8	38.0	34.0	38.0
40-44	36.593117334519775	38.0	38.0	38.0	34.4	38.0
45-49	36.59774201924522	38.0	38.0	38.0	34.0	38.0
50-54	36.44889781080642	38.0	37.4	38.0	34.0	38.0
55-59	36.40805796951802	38.0	37.0	38.0	34.0	38.0
60-64	36.28892465754465	38.0	37.0	38.0	34.0	38.0
65-69	35.96487997169551	38.0	37.0	38.0	33.0	38.0
70-74	36.21110464913226	38.0	37.0	38.0	34.0	38.0
75-79	36.00608303355994	38.0	37.0	38.0	32.6	38.0
80-84	34.81056407001411	38.0	37.0	38.0	24.2	38.0
85-89	34.86001582256857	38.0	37.0	38.0	24.8	38.0
90-94	34.80498183137879	38.0	37.0	38.0	24.6	38.0
95-99	34.62937956398346	38.0	36.8	38.0	24.2	38.0
100-104	34.63777767317133	38.0	36.6	38.0	24.0	38.0
105-109	34.422704322962616	38.0	36.0	38.0	23.6	38.0
110-114	34.24574483754534	38.0	36.0	38.0	22.8	38.0
115-119	34.29979940870363	38.0	36.0	38.0	23.0	38.0
120-124	34.091119773199736	38.0	35.4	38.0	22.2	38.0
125-129	33.951942516395796	38.0	35.0	38.0	21.8	38.0
130-134	33.82902180993785	38.0	34.6	38.0	21.2	38.0
135-139	33.51205688835978	38.0	34.0	38.0	19.0	38.0
140-144	33.415769118051244	38.0	34.0	38.0	19.0	38.0
145-149	33.18563031373329	37.2	33.8	38.0	18.6	38.0
150-154	32.906150909645845	37.0	32.8	38.0	18.0	38.0
155-159	32.69603813208656	37.0	31.2	38.0	17.8	38.0
160-164	32.38381421085319	37.0	30.8	38.0	13.4	38.0
165-169	31.779710061301067	36.8	28.6	38.0	11.6	38.0
170-174	31.092459984238168	36.4	26.4	38.0	11.0	38.0
175-179	31.237700230657214	36.4	27.4	38.0	11.0	38.0
180-184	31.29381811334851	36.2	28.0	38.0	11.0	38.0
185-189	30.936668119346507	35.6	26.8	38.0	10.6	38.0
190-194	30.248737155551645	35.0	24.0	37.8	10.2	38.0
195-199	29.76922221901654	35.0	23.4	37.2	10.0	38.0
200-204	29.01239414291636	34.0	21.8	37.0	10.0	37.8
205-209	28.31732814215409	33.0	21.0	37.0	10.0	37.2
210-214	27.720775700664944	32.0	20.0	37.0	9.8	37.0
215-219	26.712130686520293	30.0	18.8	36.8	9.2	37.0
220-224	25.540854874247024	28.4	17.0	35.8	8.8	37.0
225-229	24.584854977503706	26.6	15.2	34.4	8.6	37.0
230-234	23.65105192748344	24.8	14.0	33.4	8.2	37.0
235-239	22.473352030891974	23.4	12.2	32.0	8.0	36.4
240-244	21.6229126585104	21.6	10.6	31.2	8.0	36.0
245-249	20.660838535395023	20.4	9.4	30.0	7.8	35.8
250-254	19.617496845356406	18.8	9.0	28.4	7.4	34.8
255-259	18.626855545415477	17.6	8.6	26.6	7.0	33.6
260-264	17.990239813652053	16.6	8.4	25.6	7.2	32.6
265-269	17.357413602246137	15.2	8.0	24.6	7.0	31.8
270-274	16.499446513796155	14.0	8.0	23.0	7.0	30.8
275-279	15.58617970003933	13.0	8.0	21.0	7.0	29.4
280-284	14.901075843553974	12.4	8.0	20.0	7.0	27.6
285-289	14.42806911516613	12.0	8.0	19.2	7.0	26.8
290-294	13.997061739905522	12.0	8.0	19.0	7.0	26.0
295-299	13.405667273552774	11.0	8.0	17.8	7.0	24.8
300	11.922578744611291	10.0	8.0	13.0	7.0	21.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.12160669228417476
1101	2	-0.11663836219944557
1101	3	-0.05039155294745967
1101	4	-0.05932801677137434
1101	5	-0.04124479495147426
1101	6	0.10849644649919554
1101	7	0.08431640351903269
1101	8	0.023549938948491445
1101	9	0.06401534843224255
1101	10-14	0.04140911845574635
1101	15-19	0.07058242745193155
1101	20-24	-0.01891101793241745
1101	25-29	-0.0644931368311461
1101	30-34	0.03261849127802208
1101	35-39	0.10211904114385106
1101	40-44	0.013915543063895086
1101	45-49	-0.07263705796002284
1101	50-54	0.044059146196872234
1101	55-59	-0.033643276705554115
1101	60-64	0.0293007219103103
1101	65-69	0.130932156126498
1101	70-74	0.007783644841424575
1101	75-79	0.0658468092721094
1101	80-84	0.07236234611921333
1101	85-89	0.050065519178282614
1101	90-94	0.15810185093570794
1101	95-99	0.01725577103503184
1101	100-104	0.14318099720568256
1101	105-109	0.1096540946085014
1101	110-114	0.22740808799899526
1101	115-119	0.09430164868687285
1101	120-124	0.057397726574833996
1101	125-129	0.10002033746024352
1101	130-134	-0.10541187775338301
1101	135-139	-0.23200363072489694
1101	140-144	-0.146987503886578
1101	145-149	0.11902365330291076
1101	150-154	-0.1441282426071382
1101	155-159	-0.018221732541796598
1101	160-164	-0.15389627180222476
1101	165-169	0.29453186634986395
1101	170-174	0.8662651529854202
1101	175-179	0.21839749999952218
1101	180-184	-0.3026264865269539
1101	185-189	-0.4446454212394144
1101	190-194	-0.7769568533567615
1101	195-199	-0.8724603868855851
1101	200-204	-0.7118552753709331
1101	205-209	-0.5781140543940104
1101	210-214	-1.0851124908008636
1101	215-219	-0.8903672095554747
1101	220-224	-1.1401682467252208
1101	225-229	-0.9485711079384593
1101	230-234	-0.8381107693633965
1101	235-239	-1.0667701074983817
1101	240-244	-0.917267847672651
1101	245-249	-1.1607704612080774
1101	250-254	-0.344722926840511
1101	255-259	-0.2533719342949645
1101	260-264	-0.2959396877611482
1101	265-269	-0.7101547434949751
1101	270-274	-0.5140946893144474
1101	275-279	-0.37078484545915913
1101	280-284	-0.24492972021203485
1101	285-289	-0.4120625113763339
1101	290-294	-0.36103266686310675
1101	295-299	-0.34968668213183207
1101	300	0.5008274905500567
1102	1	0.10219182541497673
1102	2	0.003250771627151039
1102	3	0.005138635930414637
1102	4	0.1854778810073725
1102	5	0.09733446509223853
1102	6	0.17705152527293677
1102	7	0.07801198976949308
1102	8	0.0981193819308146
1102	9	0.08700908078533587
1102	10-14	0.08416267219111262
1102	15-19	0.0573849533654851
1102	20-24	-0.033869403836575884
1102	25-29	-0.00394505946993462
1102	30-34	0.08393281164921262
1102	35-39	0.01883195966129847
1102	40-44	0.06084643868219075
1102	45-49	-0.05215038436945463
1102	50-54	-0.04249484214144417
1102	55-59	0.1055685615894788
1102	60-64	0.01307479122993982
1102	65-69	0.11070982671128604
1102	70-74	-0.0025208757519337155
1102	75-79	0.1072735513369949
1102	80-84	-0.022219509600788
1102	85-89	-0.09549152206501077
1102	90-94	-0.11174308411739275
1102	95-99	0.03272549888182397
1102	100-104	-0.2426594315522621
1102	105-109	-0.024815072308250308
1102	110-114	-0.037174914720807806
1102	115-119	0.016082860829982337
1102	120-124	-0.19190309062324928
1102	125-129	-0.07171991264856814
1102	130-134	0.02842433381623266
1102	135-139	-0.03758408333416696
1102	140-144	0.06392621501676388
1102	145-149	-0.02529048009159851
1102	150-154	-0.11489640252090538
1102	155-159	0.018013279863083653
1102	160-164	0.01191565479606993
1102	165-169	0.3126269981998462
1102	170-174	1.1361992083525116
1102	175-179	0.6323428179620123
1102	180-184	0.23059945829449902
1102	185-189	0.22921489303853093
1102	190-194	0.10189281582021437
1102	195-199	0.1546594023178436
1102	200-204	0.41572321948250135
1102	205-209	0.006335393553413837
1102	210-214	0.43986039758034323
1102	215-219	0.20847494912959164
1102	220-224	0.07557663537733816
1102	225-229	0.27902162228247107
1102	230-234	0.057509985362475646
1102	235-239	0.15423519855416856
1102	240-244	0.33534116837434524
1102	245-249	0.28840377749044066
1102	250-254	0.6529970889346615
1102	255-259	0.7452433698249692
1102	260-264	0.7081144066353673
1102	265-269	0.39198467826000183
1102	270-274	0.5634493484077048
1102	275-279	0.8831074276902058
1102	280-284	1.081891880465479
1102	285-289	0.7640143407533913
1102	290-294	0.47290715178522547
1102	295-299	0.3314103524808498
1102	300	0.6498265368118599
1103	1	-0.02603641691252534
1103	2	0.11419025235394287
1103	3	0.1192159791546672
1103	4	0.11928541500740408
1103	5	0.0846625086623547
1103	6	0.17071319952994912
1103	7	0.04219027670001907
1103	8	-0.0037007348195814416
1103	9	0.008130573187173695
1103	10-14	0.11634039571492139
1103	15-19	-0.018628630996843754
1103	20-24	-0.043298954519713106
1103	25-29	-0.007968722552710972
1103	30-34	0.09610458810194444
1103	35-39	0.005308007050075503
1103	40-44	-0.014548650289114562
1103	45-49	-0.11926808942602918
1103	50-54	-0.11955949306401692
1103	55-59	-0.04915388234373097
1103	60-64	-0.24539681150570658
1103	65-69	0.05455726868822097
1103	70-74	-0.0498999389317305
1103	75-79	-0.007526517523835707
1103	80-84	0.3574734337298864
1103	85-89	0.23472366800650946
1103	90-94	0.2618240630100175
1103	95-99	0.17834078598450276
1103	100-104	0.16095482263806815
1103	105-109	0.10324007895080456
1103	110-114	0.226243086124299
1103	115-119	0.11567783398991338
1103	120-124	0.20120300199455698
1103	125-129	0.2034110011658541
1103	130-134	0.22162902261226236
1103	135-139	0.29298010590789403
1103	140-144	0.19774877319633077
1103	145-149	0.42502299315440695
1103	150-154	0.17997377917775736
1103	155-159	0.3128709414121431
1103	160-164	0.37526479481829966
1103	165-169	0.7952909730343407
1103	170-174	1.7177369957290658
1103	175-179	1.0699013887869278
1103	180-184	0.5946685069196285
1103	185-189	0.7358364871665835
1103	190-194	0.6499097806229379
1103	195-199	0.6575161874564301
1103	200-204	0.8125966251196957
1103	205-209	1.0248173254124957
1103	210-214	0.9637929765746662
1103	215-219	0.7464510669441964
1103	220-224	0.5802479031989876
1103	225-229	0.5401487487516299
1103	230-234	0.6870112139021565
1103	235-239	0.6828828658937951
1103	240-244	0.7789053419662224
1103	245-249	1.0537454993240125
1103	250-254	0.7685430457088458
1103	255-259	1.1034548664523527
1103	260-264	0.6163689412770523
1103	265-269	0.4085610433475608
1103	270-274	0.6895745186034894
1103	275-279	1.2289962800619918
1103	280-284	1.3429905235942616
1103	285-289	0.7813290816645466
1103	290-294	0.5156021139900222
1103	295-299	0.5169592827905678
1103	300	1.078976575154817
2116	1	0.19189543437026657
2116	2	0.0927613379639709
2116	3	0.06829810011944204
2116	4	0.09617919071521897
2116	5	0.11364892053156694
2116	6	0.2741174344173558
2116	7	-0.04535793795723464
2116	8	0.04973631004205714
2116	9	0.10158458320621833
2116	10-14	0.11828946823295183
2116	15-19	-0.01519718783812607
2116	20-24	-0.03476964160858387
2116	25-29	0.07029410934818969
2116	30-34	0.07243563273951281
2116	35-39	-0.0069955732383348845
2116	40-44	0.1270387122690977
2116	45-49	0.025940533995488124
2116	50-54	0.09543834627914549
2116	55-59	0.14091701921033462
2116	60-64	-0.06790925528861891
2116	65-69	0.26435448704260267
2116	70-74	0.08349389279311481
2116	75-79	0.14931572251714442
2116	80-84	0.20850910857504346
2116	85-89	0.21768104704973723
2116	90-94	0.18638000801729504
2116	95-99	0.12497256204442664
2116	100-104	0.2933646752332635
2116	105-109	0.2903820901222005
2116	110-114	0.3253826645561446
2116	115-119	0.2568327450828889
2116	120-124	0.412984183473732
2116	125-129	0.3614046312293908
2116	130-134	0.30809882943353983
2116	135-139	0.37450729017190554
2116	140-144	0.40980459816712056
2116	145-149	0.4211715823255133
2116	150-154	0.3391191193611718
2116	155-159	0.3752924775591282
2116	160-164	0.5740381819279747
2116	165-169	0.7223980935989971
2116	170-174	1.7386739205542305
2116	175-179	1.1275131427601401
2116	180-184	0.8524543938292517
2116	185-189	0.9097459956571576
2116	190-194	0.9993529713529234
2116	195-199	0.6852180488222821
2116	200-204	0.8138395629728024
2116	205-209	0.7564045279220544
2116	210-214	0.8170910992039389
2116	215-219	0.821728031141312
2116	220-224	1.353338282674347
2116	225-229	0.4773834280941536
2116	230-234	0.570171854736973
2116	235-239	0.45120411555368634
2116	240-244	0.662663604478027
2116	245-249	0.7387185311256808
2116	250-254	1.0580582583334852
2116	255-259	1.331360309707101
2116	260-264	1.0582143022667942
2116	265-269	0.87272294856675
2116	270-274	0.9106231573896828
2116	275-279	0.9530375085954645
2116	280-284	1.0142720331731745
2116	285-289	0.7626526840396686
2116	290-294	0.45174654189610486
2116	295-299	0.46171976997867326
2116	300	0.9003942861389564
1117	1	-0.16840929826845752
1117	2	-0.13044251600763346
1117	3	-0.167789670562847
1117	4	-0.18523435900389273
1117	5	-0.16674992071617112
1117	6	-0.18578397561129378
1117	7	-0.05498486454291651
1117	8	0.03415237252504966
1117	9	-0.11616321212355984
1117	10-14	-0.09360310710994924
1117	15-19	-0.07376987393470102
1117	20-24	-0.09279047994343159
1117	25-29	-5.911803293656703E-5
1117	30-34	6.24362113235577E-4
1117	35-39	-0.10056769916462116
1117	40-44	0.012682988128965178
1117	45-49	0.10423473543272621
1117	50-54	0.1469941792523599
1117	55-59	0.04474442274101165
1117	60-64	0.038332002053614644
1117	65-69	0.27681715569386967
1117	70-74	0.13066503281967812
1117	75-79	0.18546783275864698
1117	80-84	0.06832089135700414
1117	85-89	0.0653451369330611
1117	90-94	0.024309938716228885
1117	95-99	0.21461197242518892
1117	100-104	0.12626555710134824
1117	105-109	0.21363555917679378
1117	110-114	0.11957641945762987
1117	115-119	0.14901116732324482
1117	120-124	0.22154198504541256
1117	125-129	0.23471043619409926
1117	130-134	0.1634369322167828
1117	135-139	0.025183495738403394
1117	140-144	0.27063012912854134
1117	145-149	0.45315858637474093
1117	150-154	0.40833536109866486
1117	155-159	0.4924189640110015
1117	160-164	0.6287111225019117
1117	165-169	1.0138220464806693
1117	170-174	1.532426261265794
1117	175-179	1.0390539311598097
1117	180-184	0.7925216142642704
1117	185-189	0.9975314024208188
1117	190-194	1.2134126054817003
1117	195-199	1.138307147908403
1117	200-204	1.2266079245547203
1117	205-209	1.102331449706277
1117	210-214	0.862550038721551
1117	215-219	1.1930047392605871
1117	220-224	1.7761801065888214
1117	225-229	1.2942730425369469
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2108	205-209	-0.6560190963232664
2108	210-214	-0.24916151272710962
2108	215-219	-0.3399033004686274
2108	220-224	-0.8710933693875305
2108	225-229	-0.7063752749014114
2108	230-234	-0.645954476005766
2108	235-239	-0.7230896926211408
2108	240-244	-0.8184318786363072
2108	245-249	-9.76709171975898E-4
2108	250-254	-0.37749902245013445
2108	255-259	0.5413465170593241
2108	260-264	-0.039125120754537335
2108	265-269	-0.490270799316864
2108	270-274	-0.16007019158082514
2108	275-279	-0.4292775088724703
2108	280-284	0.30808119908964393
2108	285-289	-0.27091713401300943
2108	290-294	-0.21603399808192236
2108	295-299	1.042279900141624
2108	300	1.420565414080217
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	49.0
11	124.0
12	235.0
13	546.0
14	789.0
15	1172.0
16	2438.0
17	6143.0
18	22364.0
19	21289.0
20	9346.0
21	6700.0
22	7412.0
23	9422.0
24	11838.0
25	15680.0
26	20856.0
27	27549.0
28	37597.0
29	51581.0
30	68638.0
31	82048.0
32	91440.0
33	84978.0
34	56122.0
35	20187.0
36	2000.0
37	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.65391699680967	25.07072323715783	11.86586349162482	25.40949627440768
2	38.887682540357424	28.79327859716682	19.965439379973972	12.353599482501789
3	38.7475267746104	19.94752130807999	27.116572242477066	14.188379674832548
4	39.12927281479243	18.9427426494147	19.420912212077084	22.507072323715782
5	34.95633608836343	19.79931759478736	30.276834210762082	14.967512106087133
6	34.54163901766449	19.821639260621392	30.580682192625346	15.056039529088775
7	15.51720210825856	49.990205799685064	19.74267826583434	14.749913826222036
8	26.751985033854524	26.70673430991887	30.952102564258304	15.5891780919683
9	21.972415280167276	19.83120568883598	31.01375287941897	27.182626151577775
10-14	26.408231380010417	33.7796654179694	23.393075424453308	16.41902777756688
15-19	23.844246400821195	31.324039219318717	23.714355564396488	21.1173588154636
20-24	26.34536628031457	32.21705770074694	24.944173058204882	16.493402960733608
25-29	20.75878479029023	28.615798576576225	34.48445303567063	16.140963597462925
30-34	19.76670063001763	27.097287537980996	32.90423094268798	20.231780889313388
35-39	20.853295027127658	27.944690860113003	27.34780647874962	23.854207634009718
40-44	21.31397169248337	27.98878753874024	30.900474221512926	19.79676654726347
45-49	20.52187143631568	30.856316803658927	26.968611486091476	21.653200273933912
50-54	22.849307496890912	27.194379191955697	29.609796022491736	20.346517288661655
55-59	24.474689204969078	24.36459935646789	29.529134329355422	21.631577109207612
60-64	20.806009539095562	26.44746892049691	29.335254717539815	23.41126682286771
65-69	19.16307419448397	24.696326643413666	33.485474973160855	22.655124188941514
70-74	19.603129892354907	30.3185620595457	27.028955907876817	23.049352140222577
75-79	18.892237982364364	30.269697351617864	29.69282654547166	21.145238120546107
80-84	22.238331614919378	27.80981940709404	26.5383044341154	23.413544543871183
85-89	20.0268467382276	30.417506259936555	26.059345261505147	23.496301740330694
90-94	19.291173223719277	29.986561446079513	29.12506662333935	21.59719870686186
95-99	21.144175184077817	29.416037889129655	26.68529336287284	22.754493563919684
100-104	21.315125737791796	29.428975344429375	27.050609442216494	22.20528947556233
105-109	22.500421378385642	28.60726471521654	24.52367539135043	24.368638515047383
110-114	20.901157537813965	28.591685103552788	28.30639295546448	22.200764403168765
115-119	20.774242923500463	31.084240752073107	25.53054955333891	22.610966771087522
120-124	19.182358898980038	29.497762518734255	28.26363253982595	23.056246042459758
125-129	20.011965627671575	27.031051411200007	30.064854309372212	22.8921286517562
130-134	23.296287466612405	28.469872584287064	26.782202799167266	21.451637149933262
135-139	22.409798452060805	29.107573726032683	25.772959807335173	22.709668014571342
140-144	20.71487032934327	29.924060781744217	26.817097484940465	22.54397140397204
145-149	19.87965125954973	28.796111716478922	28.923755240278382	22.40048178369297
150-154	21.78935386805735	27.106649611086613	26.732951404959483	24.371045115896553
155-159	20.596762902909866	27.759679175404255	29.585925506375343	22.05763241531054
160-164	22.768136434922152	28.481209099710757	27.280515700487495	21.470138764879596
165-169	19.18281444318073	26.696104945235994	29.134876008460974	24.9862046031223
170-174	20.101282660621088	29.224132302183726	26.794077317998706	23.88050771919648
175-179	20.401820354625976	29.13186941673639	27.86557801725905	22.60073221137858
180-184	20.02359718959598	28.72394476982111	27.10943538333285	24.143022657250064
185-189	22.43205937866808	27.380119747385557	27.6555114015121	22.53230947243426
190-194	20.158717721646617	29.55404638777635	27.790968317194913	22.49626757338212
195-199	21.049391620719973	29.03984948819609	27.322994504618457	22.587764386465476
200-204	23.19224117117377	27.966435503292825	27.833325487849876	21.00799783768353
205-209	22.140912619307063	28.320341208783866	28.496910194596392	21.04183597731268
210-214	22.871446945044667	27.536614365130824	28.627460508113998	20.964478181710508
215-219	23.031919982142668	26.301664406661274	29.330760014759633	21.33565559643643
220-224	20.21021846381385	26.81375682746871	28.10009217177661	24.87593253694084
225-229	22.47794786448471	28.181057561046718	27.325181116781792	22.01581345768678
230-234	21.500410749020958	27.228787963914826	28.4113503393045	22.85945094775971
235-239	22.43880143283836	27.018903565848156	26.52017377492776	24.022121226385728
240-244	23.533511663757256	29.218431688393093	24.998815584718493	22.249241063131155
245-249	22.365823251887093	27.829893721537978	26.10514263848164	23.69914038809329
250-254	22.06176268273017	27.50937282192929	26.628775512373338	23.800088982967203
255-259	21.92036841996572	27.415265715976002	27.752581114225013	22.91178474983327
260-264	21.976424069133387	28.927633766758333	26.65298009423691	22.44296206987137
265-269	22.21030046176997	27.880155431681274	26.72857006193883	23.180974044609925
270-274	21.328859280531493	29.20230541879102	25.95822233236272	23.510612968314764
275-279	21.12318978123249	28.83212133267937	24.87720806070278	25.16748082538535
280-284	19.680997581060293	29.076353763478412	27.113049367325033	24.129599288136262
285-289	22.324368729623885	27.769002646711805	26.585438074080596	23.321190549583708
290-294	21.01434508687987	29.20624459990312	26.626740748276905	23.152669564940105
295-299	20.67089512916956	30.309937089345883	24.23267375594675	24.78649402553781
300	12.323381717189049	39.943937693701194	23.394472426668774	24.33820816244099
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.25
6	0.0
7	0.25
8	0.0
9	0.0
10	0.25
11	0.25
12	0.5
13	0.5
14	0.75
15	1.75
16	2.5
17	1.5
18	1.5
19	1.0
20	1.75
21	2.0
22	3.5
23	3.75
24	6.75
25	10.25
26	10.0
27	15.75
28	22.75
29	27.0
30	37.25
31	52.5
32	70.5
33	107.75
34	168.0
35	304.25
36	620.5
37	1591.75
38	4126.25
39	7760.0
40	12492.5
41	17663.75
42	24516.0
43	28584.25
44	29322.0
45	24671.75
46	17636.5
47	13092.5
48	10804.5
49	9257.25
50	7732.5
51	4899.5
52	1984.75
53	751.75
54	316.25
55	186.75
56	140.0
57	113.25
58	79.25
59	52.25
60	39.0
61	27.75
62	23.25
63	19.25
64	14.25
65	14.75
66	14.0
67	9.25
68	11.25
69	8.5
70	8.0
71	5.75
72	6.25
73	3.75
74	5.0
75	5.0
76	3.25
77	3.25
78	2.5
79	2.5
80	2.0
81	3.25
82	4.25
83	3.25
84	2.5
85	2.75
86	2.0
87	1.0
88	1.5
89	1.0
90	0.5
91	0.5
92	0.5
93	0.25
94	1.25
95	0.5
96	0.25
97	0.0
98	0.0
99	0.25
100	0.6666666666666666
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	3.0369613379636874E-5
150-154	3.0369613379636874E-5
155-159	0.0
160-164	3.0369613379636874E-5
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	3.0369613379636874E-5
195-199	0.0
200-204	0.0
205-209	3.0369613379636874E-5
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	3.0369613379636874E-5
245-249	0.0
250-254	0.0
255-259	3.0369613379636874E-5
260-264	0.0
265-269	0.0
270-274	3.0369613379636874E-5
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	658553.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.41268992899082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.06982053572025	9.264120862966585
2	2.9661521834906788	0.795681590387191
3	1.7882918091105604	0.7195741061446195
4	1.268343943756147	0.6804761616365829
5	1.153047050335978	0.7732731279846972
6	1.2058355760823394	0.9704099212403063
7	1.2136949312934493	1.1395239647278799
8	1.305127772017433	1.400421929902756
9	1.1918776629970234	1.438765697436236
>10	18.021883359499647	48.89256361174509
>50	0.7164151719152833	6.007319053050667
>100	0.06671538135570461	1.404693835377129
>500	0.004523410077297836	0.46352674728001597
>1k	0.014701030421067029	5.927393379195354
>5k	0.009046787951425378	8.009769686239169
>10k+	0.004523393975712689	12.11248632468568
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT	36930	5.607749110549949	Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	18014	2.735391077103893	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	14135	2.146372425605836	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	10778	1.636618465028631	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	8370	1.270968319937803	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT	7795	1.183655681471347	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	7277	1.1049983828180876	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	6780	1.02952989356969	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	5840	0.8867927106853966	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	5678	0.8621933238478907	No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	5542	0.8415419867497377	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	5526	0.8391124176793668	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	4837	0.7344890995865178	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	4665	0.70837123208003	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	4271	0.6485430937221454	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	4269	0.648239397588349	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	4090	0.6210585936135741	No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG	3322	0.5044392782357684	No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	2537	0.3852385457206937	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	2474	0.37567211750610807	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1978	0.30035547632460863	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	1914	0.2906372000431248	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1898	0.2882076309727539	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	1443	0.21911676053408	No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG	1381	0.2097021803863926	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	989	0.15017773816230431	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCGCCTGTGTAGATCT	862	0.13089303366623492	Illumina Single End PCR Primer 1 (96% over 32bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.003948049739352793	0.0	0.0
2	0.006681314943520112	0.0	0.0
3	0.01290708568634567	0.0	0.0
4	0.020803185165051256	0.0	0.0
5	0.025358627171996787	0.0	0.0
6	0.03097700564722961	0.0	0.0
7	0.04297300293218617	0.0	0.0
8	0.054817152150244554	0.0	0.0
9	0.06969826270626661	0.0	0.0
10-14	0.11258015679831387	0.0	0.0
15-19	0.17152757636818905	0.0	0.0
20-24	0.23232754235422207	0.0	0.0
25-29	0.27044140714566633	0.0	0.0
30-34	0.30785677082937896	0.0	0.0
35-39	0.3340353775626259	0.0	0.0
40-44	0.3606391588831878	0.0	0.0
45-49	0.38794144131148134	0.0	0.0
50-54	0.41424152649824697	0.0	0.0
55-59	0.4418171354469572	0.0	0.0
60-64	0.4708504858378901	0.0	0.0
65-69	0.5057755412244724	0.0	0.0
70-74	0.548718174543279	0.0	0.0
75-79	0.598979884686578	0.0	0.0
80-84	0.6535540799297854	0.0	0.0
85-89	0.7160243746516985	0.0	0.0
90-94	0.7986297230443108	0.0	0.0
95-99	0.8923807195472498	0.0	0.0
100-104	0.9946048381831076	0.0	0.0
105-109	1.1096753032785518	0.0	0.0
110-114	1.2458222800594636	0.0	0.0
115-119	1.402347267418112	0.0	0.0
120-124	1.5806472675699603	0.0	0.0
125-129	1.7790823213925073	0.0	0.0
130-134	2.001175304037792	0.0	0.0
135-139	2.257403732121788	0.0	0.0
140-144	2.551320850409914	0.0	0.0
145-149	2.89953883742083	0.0	0.0
150-154	3.2971378157870364	0.0	0.0
155-159	3.749037662876033	0.0	0.0
160-164	4.212113527688736	0.0	0.0
165-169	4.730887263439692	0.0	0.0
170-174	5.356227972539796	0.0	0.0
175-179	6.087892698082007	0.0	0.0
180-184	7.307293414501187	0.0	0.0
185-189	9.265586824446931	0.0	0.0
190-194	10.184905391061918	0.0	0.0
195-199	11.186282653028686	0.0	0.0
200-204	12.23798236436551	0.0	9.11088401389106E-5
205-209	13.334203928916883	0.0	1.5184806689818434E-4
210-214	14.465441657694976	0.0	1.5184806689818434E-4
215-219	15.620428424135946	0.0	1.5184806689818434E-4
220-224	16.788777820463956	0.0	1.5184806689818434E-4
225-229	17.972251284255027	0.0	1.822176802778212E-4
230-234	19.137988893832386	0.0	3.036961337963687E-4
235-239	20.292823812206457	0.0	4.2517458731491616E-4
240-244	21.349261183230507	0.0	6.073922675927374E-4
245-249	22.313891213007913	0.0	6.073922675927374E-4
250-254	23.24772645481837	0.0	6.073922675927374E-4
255-259	24.150068407554137	0.0	6.073922675927374E-4
260-264	25.022465921497588	0.0	6.073922675927374E-4
265-269	25.804179769889437	0.0	6.073922675927374E-4
270-274	26.496637324558538	0.0	6.073922675927374E-4
275-279	27.099960063958406	0.0	6.073922675927374E-4
280-284	27.635376347841408	0.0	6.073922675927374E-4
285-288	28.109658599991192	0.0	6.073922675927374E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	7415	0.0	290.43155	1
GGGTTAT	7445	0.0	289.8536	2
GGGGAGC	8645	0.0	272.5749	3
TTATTAG	7975	0.0	269.66898	5
GTTATTA	8040	0.0	267.67163	4
GGGGGGA	8775	0.0	267.53165	1
GGTTATT	8070	0.0	267.4052	3
GGGAGCG	6090	0.0	265.51724	4
GGAGCGA	6160	0.0	262.73865	5
TTAGTAG	8240	0.0	261.53156	8
TATTAGT	8420	0.0	255.94063	6
ATTAGTA	8500	0.0	253.53177	7
GGGAGCA	2955	0.0	250.22336	4
GGGGGAG	9705	0.0	243.40959	2
TAGTAGA	8955	0.0	240.32162	9
GAGCGAA	6755	0.0	239.37823	6
AGCGAAA	6895	0.0	234.73097	7
CGAAAGC	7110	0.0	227.6329	9
GCGAAAG	7305	0.0	221.95892	8
GCAAAAG	4215	0.0	175.07472	8
>>END_MODULE