FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n02 clinical flu pool c1.3420000000b5cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n02 clinical flu pool c1.3420000000b5cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658553
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT369305.607749110549949Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA180142.735391077103893No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC141352.146372425605836No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG107781.636618465028631No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT83701.270968319937803No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT77951.183655681471347No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT72771.1049983828180876No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT67801.02952989356969No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG58400.8867927106853966No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT56780.8621933238478907No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA55420.8415419867497377No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA55260.8391124176793668No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT48370.7344890995865178No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG46650.70837123208003No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA42710.6485430937221454No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA42690.648239397588349No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT40900.6210585936135741No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG33220.5044392782357684No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA25370.3852385457206937No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT24740.37567211750610807No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA19780.30035547632460863No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA19140.2906372000431248No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT18980.2882076309727539No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA14430.21911676053408No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG13810.2097021803863926No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG9890.15017773816230431No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCGCCTGTGTAGATCT8620.13089303366623492Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA74150.0290.431551
GGGTTAT74450.0289.85362
GGGGAGC86450.0272.57493
TTATTAG79750.0269.668985
GTTATTA80400.0267.671634
GGGGGGA87750.0267.531651
GGTTATT80700.0267.40523
GGGAGCG60900.0265.517244
GGAGCGA61600.0262.738655
TTAGTAG82400.0261.531568
TATTAGT84200.0255.940636
ATTAGTA85000.0253.531777
GGGAGCA29550.0250.223364
GGGGGAG97050.0243.409592
TAGTAGA89550.0240.321629
GAGCGAA67550.0239.378236
AGCGAAA68950.0234.730977
CGAAAGC71100.0227.63299
GCGAAAG73050.0221.958928
GCAAAAG42150.0175.074728
Produced by FastQC (version 0.11.2)