FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n02 clinical flu pool b4.3420000000b747.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n02 clinical flu pool b4.3420000000b747.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596992
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC234193.9228331367924527No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT214723.5966981132075473Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA192483.2241638078902226No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG146662.4566493353344767No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG143412.4022097448542024No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC92761.553789665523156No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG69841.169864922813036No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG63131.0574681067753002No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA61971.0380373606346482No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG61831.0356922705831904No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC50220.8412173027444254No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA45100.7554540094339622No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA43330.7258053709262435No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA36520.6117334905660378No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG35320.591632718696398No Hit
GGGGGGAGCGAAAGCAGGCCTACCAGAAGCGAATGGGAGTGCAGATGCAG26090.4370242817324186No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC25100.4204411449399657No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA23180.388279909948542No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA20030.33551538379073753No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT17680.29615137221269294No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT15540.26030499571183535No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA15140.2536047384219554No Hit
GGGGGGAGCAAAAGCAGGCCTACCAGAAGCGAATGGGAGTGCAGATGCAG14390.24104175600343053No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA10060.1685114708404803No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATAT9060.15176082761578044Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATTT8550.14321799957118353Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACA8090.13551270368782162No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT8030.13450766509433962No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA7560.1266348627787307No Hit
GGGGGGAGCAAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT6790.11373686749571184No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGAACT5980.10016884648370497Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA80400.0291.62311
GGGTTAT80900.0290.365882
GGGGGGA82600.0289.550841
TATTAGT82950.0281.06336
TTATTAG83000.0280.893985
ATTAGTA83300.0280.05887
GGTTATT83850.0279.799653
GGGGGAG90150.0267.420962
GGAGCGA61850.0265.717075
CGAAAGC62000.0264.362889
GTTATTA88350.0263.884554
TAGTAGA88150.0263.816229
GCGAAAG62800.0261.697488
GAGCGAA62700.0261.64596
AGCGAAA63550.0258.84037
TTAGTAG94300.0247.546138
GGGGAGC98700.0243.659593
GGGAGCG69400.0236.597984
GAGCAAA43200.0176.604176
GGGAGCA53350.0143.83134
Produced by FastQC (version 0.11.2)