##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE77A l01n02 clinical flu pool b3.3420000000b6db.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	693583
Sequences flagged as poor quality	0
Sequence length	300
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95256515802723	34.0	34.0	34.0	33.0	34.0
2	33.22405249263606	34.0	34.0	34.0	34.0	34.0
3	33.231435891594806	34.0	34.0	34.0	34.0	34.0
4	33.214451622949234	34.0	34.0	34.0	34.0	34.0
5	33.224260110181476	34.0	34.0	34.0	34.0	34.0
6	36.55734785887197	38.0	38.0	38.0	34.0	38.0
7	36.7549709263347	38.0	38.0	38.0	35.0	38.0
8	36.68718668133446	38.0	38.0	38.0	35.0	38.0
9	36.608948027849586	38.0	38.0	38.0	34.0	38.0
10-14	36.66828339218234	38.0	38.0	38.0	34.8	38.0
15-19	36.68133648027706	38.0	38.0	38.0	35.0	38.0
20-24	36.667877384537974	38.0	38.0	38.0	34.8	38.0
25-29	36.53945641689603	38.0	38.0	38.0	34.0	38.0
30-34	36.52692064251863	38.0	37.6	38.0	34.2	38.0
35-39	36.47297180005853	38.0	37.8	38.0	34.0	38.0
40-44	36.30249588008934	38.0	37.0	38.0	34.0	38.0
45-49	35.901234026785545	38.0	37.0	38.0	32.8	38.0
50-54	35.83402995748166	38.0	37.0	38.0	32.2	38.0
55-59	35.70682297576498	38.0	37.0	38.0	30.6	38.0
60-64	35.47047404564414	38.0	37.0	38.0	29.0	38.0
65-69	35.29027182038776	38.0	37.0	38.0	28.2	38.0
70-74	35.74796614103864	38.0	37.0	38.0	31.6	38.0
75-79	35.81227163872241	38.0	37.0	38.0	30.8	38.0
80-84	34.90262016225887	38.0	36.8	38.0	25.4	38.0
85-89	34.77029281282846	38.0	37.0	38.0	24.6	38.0
90-94	34.71212616226176	38.0	36.6	38.0	24.4	38.0
95-99	34.5281352051593	38.0	36.2	38.0	24.0	38.0
100-104	34.530286353615935	38.0	36.0	38.0	24.0	38.0
105-109	34.30272858475482	38.0	35.8	38.0	23.2	38.0
110-114	34.12967388185697	38.0	35.2	38.0	22.4	38.0
115-119	34.19089539391825	38.0	35.6	38.0	23.0	38.0
120-124	33.947332619167426	38.0	34.8	38.0	22.0	38.0
125-129	33.83180498945332	38.0	34.4	38.0	21.6	38.0
130-134	33.7011682812295	38.0	34.2	38.0	20.8	38.0
135-139	33.359005050585154	37.4	34.0	38.0	19.0	38.0
140-144	33.22547005909892	37.2	34.0	38.0	19.0	38.0
145-149	32.99368611975784	37.0	32.8	38.0	18.2	38.0
150-154	32.70968982803789	37.0	31.4	38.0	17.8	38.0
155-159	32.50863847585653	37.0	31.0	38.0	17.0	38.0
160-164	32.192519712853404	37.0	30.0	38.0	12.2	38.0
165-169	31.56869588787499	36.8	27.8	38.0	11.0	38.0
170-174	30.915085288999297	36.0	26.0	38.0	11.0	38.0
175-179	31.063605076825702	36.2	26.6	38.0	11.0	38.0
180-184	31.10811366483896	36.0	27.8	38.0	11.0	38.0
185-189	30.76677744408384	35.4	26.0	38.0	10.6	38.0
190-194	30.128246511232256	35.0	24.0	37.6	10.2	38.0
195-199	29.665763145867185	35.0	23.2	37.2	10.0	38.0
200-204	28.944551697489704	34.0	21.8	37.0	10.0	37.8
205-209	28.176017578285506	32.6	20.6	37.0	10.0	37.2
210-214	27.610390969790206	31.6	20.0	37.0	9.8	37.0
215-219	26.68383279290294	30.0	18.8	36.6	9.2	37.0
220-224	25.546008480600015	28.4	17.0	35.6	8.8	37.0
225-229	24.607303812233	26.6	15.4	34.6	8.6	37.0
230-234	23.648623164062556	24.8	14.0	33.4	8.0	37.0
235-239	22.465003034964813	23.4	12.4	32.0	8.0	36.4
240-244	21.652686412440904	21.6	10.6	31.2	8.0	36.0
245-249	20.799492202086846	20.4	9.6	30.0	8.0	36.2
250-254	19.738743596656782	18.8	9.0	28.6	7.4	34.8
255-259	18.747980558923736	17.6	8.6	27.0	7.0	33.8
260-264	18.149438207107153	16.6	8.4	25.6	7.2	32.6
265-269	17.53698432631711	15.6	8.0	24.8	7.0	32.0
270-274	16.672094904286872	14.0	8.0	23.4	7.0	30.8
275-279	15.74885601290689	13.0	8.0	21.4	7.0	29.6
280-284	15.0909543630683	13.0	8.0	20.6	7.0	28.0
285-289	14.624621134024334	12.4	8.0	19.6	7.0	27.0
290-294	14.119801667572592	12.0	8.0	19.0	7.0	26.2
295-299	13.578119705932817	11.2	8.0	18.6	7.0	25.0
300	11.998151627130422	11.0	8.0	14.0	7.0	21.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.11369374045708724
1101	2	0.07438188648826838
1101	3	0.09459449379492924
1101	4	0.005436495336695657
1101	5	0.05345556262243889
1101	6	0.263919216256852
1101	7	0.08740812248582586
1101	8	0.05607789541591046
1101	9	0.11607345394818935
1101	10-14	0.14815197399712332
1101	15-19	0.07960228027272365
1101	20-24	0.1052400836104539
1101	25-29	0.05293816795023787
1101	30-34	0.05163075155461172
1101	35-39	-0.016355353138600037
1101	40-44	0.08157213098986205
1101	45-49	0.12809737270338672
1101	50-54	0.07322369423273045
1101	55-59	0.048908970073576086
1101	60-64	0.13099463328785532
1101	65-69	0.19566483571266957
1101	70-74	0.153319146937001
1101	75-79	0.2336937882231922
1101	80-84	0.10249743028410307
1101	85-89	0.017450544090259257
1101	90-94	-0.04154861132255405
1101	95-99	0.0580749252174968
1101	100-104	-0.007489852793383989
1101	105-109	0.13139895205094376
1101	110-114	0.13126774772108973
1101	115-119	0.19558450482768563
1101	120-124	0.13731923631706167
1101	125-129	0.0994090886954595
1101	130-134	0.0966233041706559
1101	135-139	0.061640584690948685
1101	140-144	0.04551953047973001
1101	145-149	0.12228863105178789
1101	150-154	-0.16229333177095384
1101	155-159	-0.00269161272539975
1101	160-164	0.006404987569545995
1101	165-169	0.41099178990104335
1101	170-174	1.1768395284927173
1101	175-179	0.07814475152276756
1101	180-184	-0.4173349858090276
1101	185-189	-0.30519372776859655
1101	190-194	-0.78386885177596
1101	195-199	-0.8207072593109288
1101	200-204	-0.6041756735588883
1101	205-209	-0.28902434825908685
1101	210-214	-0.9818148897067651
1101	215-219	-0.8545900328024807
1101	220-224	-1.1908587169032145
1101	225-229	-0.8782315501243225
1101	230-234	-0.9295210358228303
1101	235-239	-0.926108989129812
1101	240-244	-0.8371030366046277
1101	245-249	-0.7638792812935264
1101	250-254	-0.4312635491522663
1101	255-259	-0.1953026413263821
1101	260-264	-0.4724961289431455
1101	265-269	-0.5664233683188868
1101	270-274	-0.5257398960265576
1101	275-279	-0.37565208737492384
1101	280-284	-0.13518333701283325
1101	285-289	-0.3155882538831616
1101	290-294	-0.37724299522587756
1101	295-299	-0.2968839575547939
1101	300	0.6739323961438313
1102	1	0.007736829240428733
1102	2	0.030505874181820047
1102	3	-0.050204733899335
1102	4	-0.08665425405784788
1102	5	-0.0014864826342559923
1102	6	0.034878391985913026
1102	7	0.14921369370691195
1102	8	0.1699996433534139
1102	9	0.0682983156914645
1102	10-14	0.0063520828556775655
1102	15-19	-0.08287842220619979
1102	20-24	-0.15610934598113602
1102	25-29	-0.018384313076147407
1102	30-34	0.011448294902812961
1102	35-39	-0.06415842905789759
1102	40-44	0.07262415461946148
1102	45-49	-0.08364402287251238
1102	50-54	-0.09650067151581254
1102	55-59	0.07211712763968592
1102	60-64	0.11758079552644318
1102	65-69	0.07252510517518829
1102	70-74	0.0336664184453781
1102	75-79	0.11166716820027744
1102	80-84	0.08674781883741645
1102	85-89	0.1034804981232611
1102	90-94	0.06664354426767005
1102	95-99	-0.0048893359759318855
1102	100-104	-0.03017928373847667
1102	105-109	0.18335779865929425
1102	110-114	0.14668875904133216
1102	115-119	0.1372327922142702
1102	120-124	0.019009424413297893
1102	125-129	0.11961877733801174
1102	130-134	0.18260287786390705
1102	135-139	0.22997778475873076
1102	140-144	0.16002478802392517
1102	145-149	0.14835568491248097
1102	150-154	0.17036637419526102
1102	155-159	0.2787109273460615
1102	160-164	0.35078844731646797
1102	165-169	0.5071677428038122
1102	170-174	1.2293816533399387
1102	175-179	0.5307682599957531
1102	180-184	0.49534709342403715
1102	185-189	0.5707601583139912
1102	190-194	0.41650631112144154
1102	195-199	0.23407473726599193
1102	200-204	0.4886080224145566
1102	205-209	0.2572264810498943
1102	210-214	0.5754228447628194
1102	215-219	0.5227022581957499
1102	220-224	0.002225120956541815
1102	225-229	0.33099857928548104
1102	230-234	-0.026418335702693696
1102	235-239	0.2462809651114206
1102	240-244	0.7329226857112019
1102	245-249	0.5063018045040195
1102	250-254	0.8278257061846617
1102	255-259	1.0676591326631346
1102	260-264	0.5467412364947855
1102	265-269	0.4496461669615215
1102	270-274	0.5285936519004117
1102	275-279	0.6952634176807528
1102	280-284	0.9648518887356268
1102	285-289	0.6384225937964185
1102	290-294	0.42545105150694695
1102	295-299	0.5141118552663357
1102	300	0.7940874819841781
1103	1	-0.11575817861874782
1103	2	0.07940234831598048
1103	3	0.022954493748301275
1103	4	-0.09811719554343057
1103	5	0.06008132506009645
1103	6	0.18281729642339428
1103	7	0.053294100393351584
1103	8	0.02245196163740104
1103	9	-0.05687269956622032
1103	10-14	-0.04364074086954872
1103	15-19	-0.05509663188769309
1103	20-24	-0.20547274096098533
1103	25-29	-0.11898874060619136
1103	30-34	-0.08737155513619399
1103	35-39	-0.19667598199450254
1103	40-44	-0.10072987416434387
1103	45-49	-0.16865834069815122
1103	50-54	-0.2568599713891828
1103	55-59	-0.08341809513734688
1103	60-64	-0.1642607986251292
1103	65-69	-0.024929169222403402
1103	70-74	-0.014562893627662277
1103	75-79	-0.028928670949312618
1103	80-84	0.3713954521714484
1103	85-89	0.23828739845869507
1103	90-94	0.11270099150468837
1103	95-99	0.19963877765922433
1103	100-104	0.05554131842042409
1103	105-109	0.19794010474361556
1103	110-114	0.1519200318578342
1103	115-119	0.19848715874597644
1103	120-124	0.1385389617287629
1103	125-129	0.2586501885871755
1103	130-134	0.2711357695090939
1103	135-139	0.15224877478370047
1103	140-144	0.14467774569084213
1103	145-149	0.3110142863722771
1103	150-154	0.29200376860564603
1103	155-159	0.10707341718931929
1103	160-164	0.4143789227793917
1103	165-169	0.7677593456363532
1103	170-174	1.530634029034136
1103	175-179	0.8192398060662782
1103	180-184	0.6443586194472957
1103	185-189	0.5928819512715151
1103	190-194	0.5359558676971048
1103	195-199	0.7394512717734543
1103	200-204	0.7726587517324219
1103	205-209	0.8606805841428624
1103	210-214	0.7932388728567794
1103	215-219	0.5815942413708619
1103	220-224	0.6414932335268233
1103	225-229	0.6629537206233671
1103	230-234	0.961433041331837
1103	235-239	0.651375657214512
1103	240-244	0.42465689038319
1103	245-249	0.7222995729140571
1103	250-254	0.9643395181986705
1103	255-259	1.133369618589537
1103	260-264	0.8011665673557751
1103	265-269	0.5911033905765564
1103	270-274	0.6177396520531993
1103	275-279	0.8868612731320589
1103	280-284	1.2107850243741645
1103	285-289	0.8690277852524861
1103	290-294	0.5583514934265779
1103	295-299	0.5248672392086124
1103	300	0.9820728933120435
2116	1	0.13649218027470056
2116	2	0.06118990220878118
2116	3	0.05058987682075866
2116	4	0.09226122687739036
2116	5	0.22456856891129462
2116	6	0.35090229273745877
2116	7	0.1522602168547138
2116	8	0.0634792728112501
2116	9	0.22780975174948281
2116	10-14	0.20160775683364562
2116	15-19	0.014279381449860296
2116	20-24	-0.06636711165412379
2116	25-29	0.07409322818741515
2116	30-34	0.15173072332279247
2116	35-39	0.06896806860448379
2116	40-44	0.11697059765854334
2116	45-49	0.08549241923888218
2116	50-54	0.06536349841967137
2116	55-59	0.06376166668202643
2116	60-64	-0.14478596466845772
2116	65-69	-0.02384608112803477
2116	70-74	-0.02690982748427473
2116	75-79	0.07298137113028247
2116	80-84	0.20657265323760754
2116	85-89	-0.005283441404877465
2116	90-94	0.06412598365664479
2116	95-99	0.0012540626988055692
2116	100-104	0.0276433346902607
2116	105-109	0.05505025122857887
2116	110-114	0.24698276325739243
2116	115-119	0.09031038447201922
2116	120-124	0.2033741646784506
2116	125-129	0.20612235597436523
2116	130-134	0.16790984358752326
2116	135-139	-0.034547826398373616
2116	140-144	0.18654088765744348
2116	145-149	0.28304999112611995
2116	150-154	0.1457805268895811
2116	155-159	0.1481833826109238
2116	160-164	0.28647229738889735
2116	165-169	0.6758454706570767
2116	170-174	1.5271055274274907
2116	175-179	0.9356998175179498
2116	180-184	0.6494613729036658
2116	185-189	0.5012180965736874
2116	190-194	0.7848007657666685
2116	195-199	0.20140356980845198
2116	200-204	0.6998840380535576
2116	205-209	0.4801953343400953
2116	210-214	0.5661399781662872
2116	215-219	0.8235876548972314
2116	220-224	1.155604535760375
2116	225-229	-0.03054404724164428
2116	230-234	0.5435062990966948
2116	235-239	0.491868713904978
2116	240-244	0.41454103334799797
2116	245-249	0.6078418988257503
2116	250-254	0.9599776548450762
2116	255-259	0.9906616514490061
2116	260-264	0.7590623994888759
2116	265-269	0.5370240566075424
2116	270-274	0.9230276427319666
2116	275-279	0.9402955982771779
2116	280-284	0.8457281951698192
2116	285-289	0.5037598526315712
2116	290-294	0.760011162503897
2116	295-299	0.5901772631818716
2116	300	0.9111774762137834
1117	1	0.0629535739566549
1117	2	-0.07981051207176648
1117	3	0.027525818031598703
1117	4	0.05490214525591597
1117	5	-0.03500222003239628
1117	6	-0.0654651875871437
1117	7	0.008772947338556492
1117	8	-0.006152380800990898
1117	9	0.07704298115132957
1117	10-14	0.08862589200864335
1117	15-19	-0.008819718324957648
1117	20-24	0.00911562480075645
1117	25-29	0.0019387993994328667
1117	30-34	0.12060887854967461
1117	35-39	0.0513351867998324
1117	40-44	0.1082225980124818
1117	45-49	0.11978623497368801
1117	50-54	0.17902280276822324
1117	55-59	0.06362835858445948
1117	60-64	0.13318263087359838
1117	65-69	0.25556821875834856
1117	70-74	0.26988948904071464
1117	75-79	0.32049893996560286
1117	80-84	0.20088511452697588
1117	85-89	0.15181584470828824
1117	90-94	0.12403194724566191
1117	95-99	0.18644883608382656
1117	100-104	0.3318704026211421
1117	105-109	0.36855122657507167
1117	110-114	0.3619242864152099
1117	115-119	0.3875766602187909
1117	120-124	0.38021769847395603
1117	125-129	0.2981321322921673
1117	130-134	0.30039863838270975
1117	135-139	0.2751864187818711
1117	140-144	0.33690539008424025
1117	145-149	0.6184137288625138
1117	150-154	0.5148911948774213
1117	155-159	0.7929896698309733
1117	160-164	0.9180095946507194
1117	165-169	1.1223655433407522
1117	170-174	1.7069056883347535
1117	175-179	1.0317829619248968
1117	180-184	0.7281532893936102
1117	185-189	0.9042891635599801
1117	190-194	1.0193728584928508
1117	195-199	1.1420117945957813
1117	200-204	1.0303309043757096
1117	205-209	0.9360199606525939
1117	210-214	0.7588751849227471
1117	215-219	0.9551697202410523
1117	220-224	1.5829953945164839
1117	225-229	1.3464317399060448
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2108	225-229	-0.7149459917531615
2108	230-234	-0.9250807642412937
2108	235-239	-1.0355299926248307
2108	240-244	-1.0137600493549215
2108	245-249	-0.15904307842997056
2108	250-254	-0.4551928373798191
2108	255-259	0.3789584101114194
2108	260-264	-0.18789914535775054
2108	265-269	-0.6806671492859806
2108	270-274	-0.4322961094066713
2108	275-279	-0.4056057844951457
2108	280-284	-0.07480793833611976
2108	285-289	-0.4405320129751136
2108	290-294	-0.22676844002293528
2108	295-299	0.9165717588208562
2108	300	1.5149165833291178
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	87.0
11	183.0
12	359.0
13	736.0
14	1092.0
15	1802.0
16	4949.0
17	15927.0
18	24021.0
19	11081.0
20	6911.0
21	7206.0
22	8572.0
23	11079.0
24	13974.0
25	17788.0
26	23495.0
27	30249.0
28	40197.0
29	54353.0
30	69949.0
31	85454.0
32	95564.0
33	88711.0
34	57546.0
35	20298.0
36	1989.0
37	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.63800871705333	24.234446346003292	12.694947828882771	26.432597108060605
2	39.96248466297473	27.96853440756189	19.62562519554257	12.443355733920814
3	40.08662265366942	19.86582716127702	25.679983505939447	14.367566679114107
4	40.20095648249741	19.02108327337896	19.190781780983674	21.587178463139956
5	34.48094892752562	19.4403553720319	30.975384344772	15.103311355670481
6	33.90034069462487	19.636726967068107	31.305265555816682	15.157666782490345
7	15.56194428064125	49.83570819930708	19.544452502440226	15.057895017611447
8	27.2983911082019	25.680560221343374	31.491400452433233	15.52964821802149
9	21.14094491935356	19.533062373212722	31.8959663082861	27.430026399147618
10-14	26.473111365186284	33.92643706665244	23.286816430045143	16.31363513811613
15-19	23.65438599273627	31.573034517858712	23.58073943565514	21.191840053749875
20-24	25.896568975883206	31.791724999026794	25.48984043726562	16.821865587824384
25-29	20.22471715713909	28.329817772350246	34.91169766271665	16.53376740779402
30-34	19.73295193221287	27.282185405351633	32.77733162433336	20.207531038102143
35-39	22.059883243966475	30.329866793159578	26.482108125487503	21.12814183738644
40-44	21.023093126561637	27.608260294730407	30.03115704969701	21.33748952901095
45-49	20.63931786102024	31.266020072579632	26.34539773898726	21.749264327412867
50-54	22.943843779331385	27.44435777693513	29.01135120093774	20.600447242795745
55-59	23.977288947393465	24.94738192833446	29.035515576362165	22.03981354790991
60-64	21.28463356224129	26.216934382763128	28.632708702491268	23.865723352504315
65-69	18.94435128888684	25.012233575505743	32.658585922665345	23.384829212942073
70-74	19.742179378675658	29.54213122293943	28.28042209800413	22.435267300380776
75-79	19.160129357265102	29.651534135063862	29.69891130549624	21.489425202174793
80-84	22.07623312566773	28.502140335042814	26.26353298740021	23.158093551889248
85-89	20.111825116820913	30.201403436935447	25.77032597396418	23.916445472279456
90-94	19.458406564174727	31.29047280570602	28.28941885830535	20.9617017718139
95-99	21.4444702364389	29.933490296042436	26.614464310688124	22.007575156830544
100-104	21.73069409140651	29.100136537371878	26.543499480235244	22.625669890986373
105-109	23.166023388693205	28.185465906748004	24.245086745205693	24.4034239593531
110-114	20.4568739429888	28.542481577547317	28.83894213093458	22.161702348529303
115-119	21.761461858205866	29.649861660392485	26.428444757152352	22.160231724249297
120-124	18.918168409548677	29.080038005545116	28.24746281266986	23.754330772236344
125-129	20.12275387372528	26.183254203173956	30.476208326905358	23.217783596195407
130-134	23.56750381713508	28.690899286747225	26.343292727762936	21.398304168354763
135-139	22.425290123892886	28.138521272868566	25.717844872207074	23.718343731031467
140-144	21.12785347968448	29.138228589801074	26.26240839236256	23.471509538151885
145-149	19.810231796338723	28.175402222949526	29.14552403966072	22.868841941051034
150-154	22.50104169219834	26.830588408308735	26.31370722754162	24.354662671951303
155-159	20.844686216357665	27.640383342728985	29.622929051029224	21.892001389884125
160-164	23.376236153423598	28.45231212414376	27.254964438286404	20.91648728414624
165-169	19.266158484276573	26.28761085551405	29.051000384957533	25.395230275251844
170-174	20.754257241022344	29.30853264857991	26.955389621717952	22.981820488679798
175-179	20.147466128783435	29.494754052507055	27.872799650510466	22.484980168199048
180-184	19.893076964112442	28.880177282315167	27.074827381870666	24.15191837170173
185-189	22.265107420452924	27.224715715350577	28.223096586854062	22.28708027734244
190-194	19.86193433230053	28.762469668374223	28.08632852881342	23.28926747051182
195-199	20.85582283759055	29.23316437489511	27.7148164573862	22.19619633012814
200-204	22.851857672405465	27.69915064238887	27.824788093133773	21.624203592071893
205-209	21.84315359517174	27.676399219704056	28.52728512665391	21.95316205847029
210-214	23.360412051740614	27.442318350455057	28.22693988374568	20.970329714058654
215-219	22.998141534610856	27.031919755818695	29.07631819118981	20.89362051838064
220-224	20.179329654850246	26.87560104558503	28.064038478451746	24.881030821112972
225-229	22.3802486508464	28.388700415090913	27.076269170380474	22.154781763682212
230-234	22.011496821577232	27.064907876923165	28.460703333270853	22.46289196822875
235-239	22.835853819946568	26.787940304188538	26.537472804264233	23.83873307160066
240-244	24.511586933359094	29.970717275365743	24.200737330643918	21.316958460631245
245-249	22.53728825533498	28.31629379612822	25.590015902927266	23.556402045609538
250-254	22.71431104856953	26.947978828777526	26.37717475774349	23.960535364909465
255-259	23.045720555434606	27.2923355964607	27.34343258124839	22.318511266856312
260-264	22.841447959364633	28.637956812666975	26.82525379082244	21.695341437145952
265-269	22.987155106166096	28.097401464568765	26.44185339029359	22.473590038971544
270-274	22.167008130245407	29.689078307859333	25.04637512741806	23.0975384344772
275-279	22.5934603356772	28.59187725189343	24.333093515844535	24.481568896584836
280-284	20.45266392054015	29.392790769093246	26.495516758628746	23.65902855173786
285-289	23.5399368208275	27.38469656839917	26.43075161876805	22.644614992005284
290-294	22.234628011355525	29.528866768649177	25.801237919614522	22.435267300380776
295-299	21.705953000578155	30.61020815100716	23.421854341874006	24.261984506540674
300	13.775856674687818	40.285156931470354	22.250256998801873	23.68872939503996
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.25
9	0.5
10	0.25
11	0.0
12	0.0
13	0.0
14	0.25
15	0.25
16	0.0
17	1.0
18	1.0
19	2.0
20	2.5
21	3.0
22	2.0
23	7.0
24	7.0
25	6.75
26	9.5
27	10.25
28	14.75
29	25.75
30	30.5
31	42.75
32	60.0
33	91.0
34	162.75
35	286.25
36	589.25
37	1576.75
38	3959.25
39	7058.75
40	11765.25
41	17076.5
42	24469.0
43	29563.0
44	30619.75
45	26481.25
46	18924.75
47	15025.25
48	13316.5
49	11195.5
50	9363.5
51	5850.25
52	2273.5
53	737.5
54	256.5
55	106.25
56	52.25
57	35.0
58	28.0
59	18.25
60	17.25
61	13.75
62	8.25
63	6.75
64	7.5
65	6.25
66	3.0
67	3.5
68	3.25
69	3.0
70	3.75
71	3.75
72	4.0
73	2.25
74	1.5
75	2.25
76	1.75
77	0.5
78	1.75
79	1.5
80	1.25
81	1.25
82	1.0
83	2.0
84	1.5
85	0.5
86	0.5
87	0.75
88	1.0
89	0.5
90	0.25
91	1.0
92	2.25
93	1.25
94	1.0
95	0.5
96	0.5
97	0.25
98	0.25
99	0.0
100	0.6666666666666666
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	2.8835770196213E-5
200-204	0.0
205-209	0.0
210-214	2.8835770196213E-5
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	693583.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.314423413411896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.1044626252016	8.263527656962061
2	3.280296397497674	0.8078991752055212
3	1.9654241319540677	0.7260919484345978
4	1.3198334567349883	0.650119520856868
5	1.5002255540195726	0.9237206343908729
6	1.4815777917488937	1.0946865748502204
7	1.155928906153742	0.9964218590325471
8	1.3165296391557142	1.2969842730295875
9	1.3033729859028542	1.4445258132609613
>10	18.42902109932539	46.84662086926133
>50	0.9726744269708607	7.694708834562008
>100	0.13421697727861007	2.407936864861244
>500	0.003526078444931149	0.3276881982225812
>1k	0.017630319435616708	5.62698488716664
>5k	0.008227482402182398	7.011694725921792
>10k+	0.007052127773299197	13.880388163981092
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT	29441	4.244769551733534	Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	17715	2.554128345129566	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	14387	2.074301129064582	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	13419	1.934736001314911	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT	10514	1.5158964392149172	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	10424	1.5029203426266216	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	8190	1.1808247895349222	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	7896	1.1384362073464893	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	7807	1.1256042896091742	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	7433	1.0716813993422563	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	6341	0.9142380940709331	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	5751	0.8291725719921048	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	5026	0.7246429050308326	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	4935	0.7115226295915558	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	4552	0.6563021296658079	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	3726	0.5372103987554482	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	3613	0.5209181885945878	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3259	0.4698788753472908	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	2973	0.4286437239667062	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	2585	0.372702329786053	No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG	2334	0.3365134381898057	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2258	0.32555584551524475	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1726	0.24885269679331815	No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	1685	0.2429413639030945	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1646	0.23731838871483296	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATAT	1344	0.19377637571855133	Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATTT	1236	0.17820505981259632	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG	1005	0.14489974523597032	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGAACT	984	0.14187198936536793	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	710	0.10236698419655614	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.00346029242354556	0.0	0.0
2	0.00692058484709112	0.0	0.0
3	0.011390129227504134	0.0	0.0
4	0.016003852458898214	0.0	0.0
5	0.020617575690292295	0.0	0.0
6	0.025519656623648503	0.0	0.0
7	0.034458745384474535	0.0	0.0
8	0.042965297592357364	0.0	0.0
9	0.05334617486299405	0.0	0.0
10-14	0.07892350302703496	0.0	0.0
15-19	0.1148817084617126	0.0	0.0
20-24	0.15037854157325076	0.0	0.0
25-29	0.1710537888039355	0.0	0.0
30-34	0.2024847783178077	0.0	0.0
35-39	0.21670081302454064	0.0	0.0
40-44	0.2298210884638176	0.0	0.0
45-49	0.24481568896584838	0.0	0.0
50-54	0.25885870905140407	0.0	0.0
55-59	0.2747760541997136	0.0	0.0
60-64	0.2929714251935241	0.0	0.0
65-69	0.3139061943559747	0.0	0.0
70-74	0.33593672278588144	0.0	0.0
75-79	0.36459947836091716	0.0	0.0
80-84	0.3961746467257704	0.0	0.0
85-89	0.432132852160448	0.0	0.0
90-94	0.4744060912680962	0.0	0.0
95-99	0.5260797914597101	0.0	0.0
100-104	0.583635988771351	0.0	0.0
105-109	0.6496410667504826	0.0	0.0
110-114	0.7291124494112455	0.0	0.0
115-119	0.8247895349222804	0.0	0.0
120-124	0.937393217538492	0.0	0.0
125-129	1.06654863224733	0.0	0.0
130-134	1.2152258633790045	0.0	0.0
135-139	1.3899706307680553	0.0	0.0
140-144	1.5981072200443207	0.0	8.650731058863899E-5
145-149	1.8424615366870296	0.0	1.4417885098106498E-4
150-154	2.1111820791455385	0.0	1.4417885098106498E-4
155-159	2.41811001711403	0.0	1.4417885098106498E-4
160-164	2.7534700244959867	0.0	1.4417885098106498E-4
165-169	3.1244998796106596	0.0	1.4417885098106498E-4
170-174	3.5981274050834577	0.0	1.4417885098106498E-4
175-179	4.145113129935423	0.0	1.4417885098106498E-4
180-184	5.000093716253137	0.0	1.4417885098106498E-4
185-189	6.393812997146701	0.0	1.4417885098106498E-4
190-194	7.108594068770428	0.0	2.595219317659169E-4
195-199	7.911266567952214	0.0	2.8835770196212996E-4
200-204	8.769246074370335	0.0	3.46029242354556E-4
205-209	9.678466744427126	0.0	4.32536552943195E-4
210-214	10.641466125899855	0.0	5.478796337280468E-4
215-219	11.651929185115552	0.0	5.767154039242599E-4
220-224	12.707808582390284	0.0	5.767154039242599E-4
225-229	13.810286584302096	0.0	5.767154039242599E-4
230-234	14.910169366896247	0.0	5.767154039242599E-4
235-239	16.02351845417203	0.0	5.767154039242599E-4
240-244	17.036461389624602	0.0	6.920584847091119E-4
245-249	17.96405044529638	0.0	7.20894254905325E-4
250-254	18.88267157643714	0.0	7.20894254905325E-4
255-259	19.794199108109627	0.0	7.20894254905325E-4
260-264	20.67129673016784	0.0	7.20894254905325E-4
265-269	21.489569381025778	0.0	7.20894254905325E-4
270-274	22.197833568585157	0.0	7.20894254905325E-4
275-279	22.82166662101003	0.0	7.20894254905325E-4
280-284	23.39999682806528	0.0	7.20894254905325E-4
285-288	23.89043560756247	0.0	7.20894254905325E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	8430	0.0	288.41995	1
GGGTTAT	8445	0.0	287.38544	2
GGGGGGA	8570	0.0	279.59158	1
GTTATTA	8625	0.0	279.51306	4
GGTTATT	8755	0.0	276.70587	3
TTATTAG	8810	0.0	273.6436	5
GGGGAGC	8885	0.0	270.17557	3
GGGAGCG	6405	0.0	266.6885	4
GGAGCGA	6435	0.0	265.9021	5
TTAGTAG	9145	0.0	264.10168	8
TATTAGT	9400	0.0	257.25	6
ATTAGTA	9450	0.0	255.88889	7
GGGGGAG	9525	0.0	252.7937	2
CGAAAGC	6810	0.0	248.66962	9
GAGCGAA	7080	0.0	239.39406	6
AGCGAAA	7095	0.0	238.88794	7
GCGAAAG	7160	0.0	237.12988	8
TAGTAGA	10230	0.0	236.09091	9
GGGAGCA	3040	0.0	225.81908	4
GCAAAAG	4005	0.0	171.40823	8
>>END_MODULE