FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n02 clinical flu pool b2.3420000000b661.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n02 clinical flu pool b2.3420000000b661.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712891
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT651709.1416499857622Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA155502.1812591265705414No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC144112.0214871558204552No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG132321.8561042291177756No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT67940.9530208685479267No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA63800.8949474744385889No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT60280.84557106205577No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT57530.806995739881693No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG55440.7776784950293945No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT54450.7637913790467267No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT52430.7354560514861318No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG50370.7065596283302776No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA49480.6940752513357582No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT48090.6745771794004974No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA47800.6705092363348675No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG41450.5814353105874531No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA37680.5285520507342637No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG36330.5096150743942622No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT33210.46584961796403657No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATAT28180.39529184686017915Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATTT28010.3929071905803271Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA26920.3776173356095111No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG22830.32024531099424736No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA21860.3066387428092092No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG21280.2985028566779494No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT21020.2948557353087639No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT20630.2893850532549857No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA19850.2784436891474293No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGAACT19730.27676040236165134Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCA13780.19329743256683No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCC12500.17534237351853227Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA11420.1601927924465311No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCA9280.13017417810015836Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGAGCT7720.10829144988504555Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA68300.0291.847231
GGGTTAT68800.0289.298922
GGGGAGC98700.0269.127233
GGGGGGA98750.0268.39551
TTATTAG74700.0265.465455
GGTTATT75450.0263.800783
GGAGCGA71350.0262.68355
GTTATTA75600.0262.30514
GGGAGCG71600.0262.176944
TTAGTAG78600.0253.041568
TATTAGT78450.0252.40116
GGGGGAG107400.0247.600132
ATTAGTA81200.0244.396137
CGAAAGC78500.0238.008539
GCGAAAG80250.0233.184668
GAGCGAA80500.0232.64316
AGCGAAA80950.0230.986637
TAGTAGA88000.0226.179159
GGGAGCA37200.0208.64484
GCAAAAG44150.0177.132228
Produced by FastQC (version 0.11.2)