##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE77A l01n02 clinical flu pool b1.3420000000b5a6.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	618638
Sequences flagged as poor quality	0
Sequence length	300
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.066465364235626	34.0	34.0	34.0	34.0	34.0
2	33.38928420174642	34.0	34.0	34.0	34.0	34.0
3	33.37436109647322	34.0	34.0	34.0	34.0	34.0
4	33.38971256211225	34.0	34.0	34.0	34.0	34.0
5	33.44247524400377	34.0	34.0	34.0	34.0	34.0
6	36.861324393263914	38.0	38.0	38.0	36.0	38.0
7	36.95291268884226	38.0	38.0	38.0	36.0	38.0
8	36.91048076581135	38.0	38.0	38.0	36.0	38.0
9	36.852619787339286	38.0	38.0	38.0	36.0	38.0
10-14	36.91331958269618	38.0	38.0	38.0	36.0	38.0
15-19	36.91408319566532	38.0	38.0	38.0	36.0	38.0
20-24	36.926813742447116	38.0	38.0	38.0	36.0	38.0
25-29	36.797024754379784	38.0	38.0	38.0	35.8	38.0
30-34	36.70292966161147	38.0	37.8	38.0	35.2	38.0
35-39	36.700034592120105	38.0	38.0	38.0	35.2	38.0
40-44	36.45102499361501	38.0	37.6	38.0	34.0	38.0
45-49	35.68677449493888	38.0	37.0	38.0	30.4	38.0
50-54	35.65938367833854	38.0	37.0	38.0	30.6	38.0
55-59	35.43420675742518	38.0	37.0	38.0	28.8	38.0
60-64	34.97182294007158	38.0	37.0	38.0	26.4	38.0
65-69	34.955913797729856	38.0	36.8	38.0	25.2	38.0
70-74	35.728690445785745	38.0	37.0	38.0	31.2	38.0
75-79	35.92924812248843	38.0	37.0	38.0	32.4	38.0
80-84	34.315287454052296	38.0	36.0	38.0	21.8	38.0
85-89	33.985646856481495	38.0	36.0	38.0	19.4	38.0
90-94	33.94351236102534	38.0	36.0	38.0	19.2	38.0
95-99	33.766877560059356	38.0	36.0	38.0	18.4	38.0
100-104	33.78981375214584	38.0	35.8	38.0	18.8	38.0
105-109	33.571038959779386	38.0	34.8	38.0	16.6	38.0
110-114	33.39251711016782	38.0	34.4	38.0	11.0	38.0
115-119	33.42953197184784	38.0	34.2	38.0	12.4	38.0
120-124	33.193532243412136	38.0	34.0	38.0	11.0	38.0
125-129	33.10212369754202	38.0	34.0	38.0	11.0	38.0
130-134	33.011038765804884	38.0	34.0	38.0	11.0	38.0
135-139	32.66914350557192	37.8	32.2	38.0	11.0	38.0
140-144	32.48680617744141	37.2	31.2	38.0	11.0	38.0
145-149	32.18450208360947	37.0	30.6	38.0	11.0	38.0
150-154	31.898210585188753	37.0	29.4	38.0	11.0	38.0
155-159	31.5713467326611	37.0	28.0	38.0	11.0	38.0
160-164	31.220881355493844	37.0	27.0	38.0	11.0	38.0
165-169	30.421874504960897	36.2	23.8	38.0	11.0	38.0
170-174	29.62991086871482	35.4	21.8	38.0	11.0	38.0
175-179	29.73451259056185	35.4	22.2	38.0	11.0	38.0
180-184	29.563620404824793	35.0	21.8	38.0	10.6	38.0
185-189	29.052419670307994	35.0	21.0	37.8	10.4	38.0
190-194	28.20916076930289	34.2	19.4	37.2	10.0	38.0
195-199	27.59780873467197	33.8	17.6	37.2	10.0	38.0
200-204	26.854831419990365	31.4	16.6	37.0	9.2	37.6
205-209	25.938098209292026	29.6	15.4	37.0	9.0	37.2
210-214	25.15481234583068	28.0	14.0	37.0	9.0	37.0
215-219	24.179040408122358	25.8	10.8	35.8	8.8	37.0
220-224	22.994944701101453	23.6	10.0	34.0	8.2	37.0
225-229	22.020007177056694	22.2	9.6	33.0	8.0	37.0
230-234	21.130305930123917	20.6	9.0	31.8	8.0	37.0
235-239	20.087033127612592	19.0	9.0	30.6	8.0	36.0
240-244	19.330541285856995	17.6	8.8	29.0	7.4	36.0
245-249	18.395960157636615	16.0	8.2	27.0	7.2	34.6
250-254	17.522847933686585	14.4	8.0	25.6	7.0	33.4
255-259	16.656755647082786	13.4	8.0	23.8	7.0	32.2
260-264	16.220921443558264	13.0	8.0	22.8	7.0	31.4
265-269	15.713119788955737	12.8	8.0	21.6	7.0	30.6
270-274	14.948487807085888	12.0	8.0	20.4	7.0	29.2
275-279	14.239028640335707	11.2	8.0	19.0	7.0	27.2
280-284	13.666036357288107	11.2	8.0	18.2	7.0	25.8
285-289	13.322953973082807	11.0	8.0	17.6	7.0	25.0
290-294	12.913106534031211	10.4	8.0	16.4	7.0	24.2
295-299	12.491734746329842	8.8	8.0	15.4	7.0	22.6
300	11.395704757871323	8.0	8.0	13.0	7.0	19.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.12905829415173287
1101	2	0.012950211601051365
1101	3	-0.0054441412447303605
1101	4	-0.0060192772874785305
1101	5	-3.8582727120939353E-4
1101	6	0.06389104775446697
1101	7	-0.1119664775291227
1101	8	0.03837701442324715
1101	9	0.18419136927641233
1101	10-14	0.09916526322711405
1101	15-19	0.012275985315881144
1101	20-24	0.025492485529696296
1101	25-29	0.014558721713513023
1101	30-34	0.06727103302286963
1101	35-39	-0.10973539830747825
1101	40-44	-0.028681286294435893
1101	45-49	-0.017437886484046317
1101	50-54	0.08415526337956436
1101	55-59	-0.04544131579429944
1101	60-64	-0.006836916313666563
1101	65-69	0.025045701901746042
1101	70-74	0.025593213551303506
1101	75-79	0.0965061261597171
1101	80-84	-0.029437965555743517
1101	85-89	-0.19944379807454737
1101	90-94	-0.1770478334238348
1101	95-99	-0.20813449035760812
1101	100-104	-0.12222488288928446
1101	105-109	-0.0528041606628733
1101	110-114	-0.17342908544584645
1101	115-119	-0.20054856558648737
1101	120-124	-0.08737379887234198
1101	125-129	-0.1206700407919783
1101	130-134	-0.24059239135227983
1101	135-139	-0.31556227584384544
1101	140-144	-0.3288449494345045
1101	145-149	-0.15530684148215101
1101	150-154	-0.29063461466678575
1101	155-159	-0.054887281109131436
1101	160-164	-0.20320691588640472
1101	165-169	0.1871981488570853
1101	170-174	0.7426518729675884
1101	175-179	-0.2557826726836687
1101	180-184	-0.5068144295662833
1101	185-189	-0.5449292230355383
1101	190-194	-1.1299132582884148
1101	195-199	-1.1514498121239818
1101	200-204	-0.8663568549667211
1101	205-209	-0.5582233110103516
1101	210-214	-1.2796314430289897
1101	215-219	-1.1240325103807969
1101	220-224	-1.3180735492615128
1101	225-229	-0.989414136879013
1101	230-234	-0.9157679469252535
1101	235-239	-1.0387241129847453
1101	240-244	-0.8367539730286104
1101	245-249	-0.9341717885732734
1101	250-254	-0.5035393072381318
1101	255-259	-0.3475801841035704
1101	260-264	-0.6384105140203271
1101	265-269	-0.8525255283084547
1101	270-274	-0.7030540158346472
1101	275-279	-0.430271311229248
1101	280-284	-0.2366792547879033
1101	285-289	-0.5395145724339834
1101	290-294	-0.508653907547691
1101	295-299	-0.225168026397343
1101	300	0.8732438232319275
1102	1	0.042475675110168254
1102	2	-0.019917101107544966
1102	3	0.027099232021114972
1102	4	0.02521880018668554
1102	5	0.004931442622272186
1102	6	0.12890360968094683
1102	7	0.07389351280568235
1102	8	0.19442362496396015
1102	9	0.12810780773710206
1102	10-14	0.0609055775437497
1102	15-19	0.07190281555606504
1102	20-24	-0.1377667055896339
1102	25-29	0.01707675917037932
1102	30-34	0.07830518054991131
1102	35-39	0.06374840966602591
1102	40-44	0.12134662124752538
1102	45-49	0.02776081521471241
1102	50-54	-0.057962586990804255
1102	55-59	0.11399425946845554
1102	60-64	0.0698556937692203
1102	65-69	0.07762866855117778
1102	70-74	0.09796057854238427
1102	75-79	0.28877432399662695
1102	80-84	0.02553932688985583
1102	85-89	-0.006649525408676027
1102	90-94	-0.013965625576943808
1102	95-99	-0.03752176790977302
1102	100-104	-0.05739905421191338
1102	105-109	0.022824924789304646
1102	110-114	-0.09835221606444833
1102	115-119	0.026883205772321617
1102	120-124	-0.07636706432322171
1102	125-129	0.02951492615701312
1102	130-134	0.09357166825541441
1102	135-139	0.046918188876780675
1102	140-144	0.07314298602912572
1102	145-149	0.10734403587444064
1102	150-154	-0.13704358521055582
1102	155-159	0.09872444938455871
1102	160-164	0.2957037715010813
1102	165-169	0.30097564308481495
1102	170-174	1.1278136095373448
1102	175-179	0.6096550023566785
1102	180-184	0.2814707614908656
1102	185-189	0.48584481207003094
1102	190-194	0.17691864928745815
1102	195-199	0.06486394777789428
1102	200-204	0.34923674904857194
1102	205-209	0.2835900292186153
1102	210-214	0.3748439375733561
1102	215-219	0.4052023707966619
1102	220-224	-0.10578172539413089
1102	225-229	0.11314473743672337
1102	230-234	-0.2982751463645599
1102	235-239	-0.07967980852547285
1102	240-244	0.14180151846912992
1102	245-249	0.2643468508386775
1102	250-254	0.5714754590309035
1102	255-259	0.7175590568678132
1102	260-264	0.30530838143448946
1102	265-269	0.2116869703138331
1102	270-274	0.10435872187402317
1102	275-279	0.28981180378939086
1102	280-284	0.28356190876583653
1102	285-289	0.1994300137854843
1102	290-294	0.1878479703826006
1102	295-299	0.1276024506732547
1102	300	0.7456989380131755
1103	1	-0.06234746327052676
1103	2	0.05370398978919155
1103	3	0.17263777239607236
1103	4	0.08308740109151813
1103	5	0.14738615846026448
1103	6	0.3606639589309779
1103	7	0.18345032538955763
1103	8	0.16136930095641588
1103	9	0.24821912835293602
1103	10-14	0.13933834068450324
1103	15-19	0.05921036848431527
1103	20-24	0.026738523534923786
1103	25-29	0.09821510589503646
1103	30-34	0.08790332784871424
1103	35-39	0.1784300191152539
1103	40-44	0.09873582439349349
1103	45-49	0.04068711485175669
1103	50-54	-0.051410893116823786
1103	55-59	0.13984261202526227
1103	60-64	0.0017233434093881783
1103	65-69	0.14998975842870266
1103	70-74	0.1617684966883246
1103	75-79	0.25858681727266486
1103	80-84	0.457224084572168
1103	85-89	0.13209143616136743
1103	90-94	0.07376957178216514
1103	95-99	0.16871014815244223
1103	100-104	0.07754279407269138
1103	105-109	0.13713905163591988
1103	110-114	0.1853963046623619
1103	115-119	0.12506702634568967
1103	120-124	0.19606607819094535
1103	125-129	0.15466738330763263
1103	130-134	0.2153972470829686
1103	135-139	0.17241420545597208
1103	140-144	0.2557392307985893
1103	145-149	0.1637295415452229
1103	150-154	0.0359981118909225
1103	155-159	0.15085347031803664
1103	160-164	0.30865993971638517
1103	165-169	0.6554087912612729
1103	170-174	1.2953368390809104
1103	175-179	0.7433822894970454
1103	180-184	0.4150235085507248
1103	185-189	0.3576318873275177
1103	190-194	0.19743567296149322
1103	195-199	0.2053432322909643
1103	200-204	0.20387416432656025
1103	205-209	0.5672604103473375
1103	210-214	0.6165962463719872
1103	215-219	0.27258715735665007
1103	220-224	0.2519576337479137
1103	225-229	0.4060263899193508
1103	230-234	0.36656160982290587
1103	235-239	0.4211868049156706
1103	240-244	0.2991314940646639
1103	245-249	0.4793234655821479
1103	250-254	0.40697037146359705
1103	255-259	0.8148687614661583
1103	260-264	0.22400979191085568
1103	265-269	0.0784850021019281
1103	270-274	0.4622257656491886
1103	275-279	0.573329161538517
1103	280-284	0.6776087189149234
1103	285-289	0.5960336308561534
1103	290-294	0.4310829497435549
1103	295-299	0.302276869014543
1103	300	1.1018137862354997
2116	1	0.15314504561073505
2116	2	-0.01834399605559156
2116	3	0.0629892662576026
2116	4	0.039704237524190944
2116	5	0.11436092032279532
2116	6	0.09747726102938259
2116	7	-0.05532705654393766
2116	8	-0.021212252699953638
2116	9	0.08112345012170152
2116	10-14	0.01016800578870658
2116	15-19	0.03717301780017124
2116	20-24	-0.07635475799977343
2116	25-29	-0.02040089558739311
2116	30-34	0.034434426860975975
2116	35-39	-0.10640042037284303
2116	40-44	0.053419873599317214
2116	45-49	-0.04905626412984532
2116	50-54	-0.022614987978137435
2116	55-59	-0.03652844043744352
2116	60-64	-0.2714521857709684
2116	65-69	0.07001190840393434
2116	70-74	0.05945933292920813
2116	75-79	0.040513677650672264
2116	80-84	0.2932024364323169
2116	85-89	0.05990032629041764
2116	90-94	-0.014753179169773034
2116	95-99	-0.050065993613159776
2116	100-104	0.10300582012558834
2116	105-109	0.11776488938014751
2116	110-114	0.16881071757625676
2116	115-119	0.1679105005750614
2116	120-124	0.08803700058023622
2116	125-129	0.2737972613313602
2116	130-134	0.24046435674915045
2116	135-139	0.0733036653989032
2116	140-144	0.16012675730538461
2116	145-149	0.18762981185856376
2116	150-154	0.2791227148749691
2116	155-159	0.25913411454199675
2116	160-164	0.2149186776725145
2116	165-169	0.5171302163872227
2116	170-174	1.30971939770588
2116	175-179	0.9443064308790703
2116	180-184	0.6749868514953583
2116	185-189	0.7043860002505724
2116	190-194	0.7024428507936413
2116	195-199	0.4452170812601466
2116	200-204	0.6807633473948123
2116	205-209	0.4607255908314194
2116	210-214	0.5201881186577744
2116	215-219	0.6530800375672463
2116	220-224	1.0812915698605252
2116	225-229	0.2744259027929452
2116	230-234	0.5821989731971442
2116	235-239	0.5464333063083338
2116	240-244	0.6839552279938523
2116	245-249	0.5550700358329657
2116	250-254	0.8537055213544669
2116	255-259	1.0448540741290628
2116	260-264	0.8936819910241347
2116	265-269	0.8453285368976502
2116	270-274	0.8935195275326233
2116	275-279	0.6645876363194851
2116	280-284	0.7335000571353856
2116	285-289	0.5306028780093577
2116	290-294	0.4429835863390359
2116	295-299	0.4118577427416614
2116	300	0.6916953141511737
1117	1	0.1618882019607284
1117	2	0.015747258400111264
1117	3	0.154224545987951
1117	4	0.022454187232497702
1117	5	-0.047081922988553515
1117	6	0.07012219879069903
1117	7	0.10361097106656558
1117	8	0.20844132075993116
1117	9	0.1329607973833049
1117	10-14	0.1541707758116715
1117	15-19	0.07384940993932787
1117	20-24	0.06881239132241745
1117	25-29	0.11810929806816972
1117	30-34	0.27375507655728626
1117	35-39	0.16602402992810994
1117	40-44	0.20518485871442493
1117	45-49	0.41056196765767794
1117	50-54	0.4304303005948711
1117	55-59	0.4068943469813817
1117	60-64	0.2562264076246805
1117	65-69	0.5102189943947693
1117	70-74	0.43043270510653997
1117	75-79	0.47838216379116005
1117	80-84	0.3042651613881304
1117	85-89	0.23219286039831388
1117	90-94	0.188182987286325
1117	95-99	0.21159798841786426
1117	100-104	0.2716799574602575
1117	105-109	0.24053805286504115
1117	110-114	0.2378545531173586
1117	115-119	0.34480005712535444
1117	120-124	0.3199613705592057
1117	125-129	0.33816961378132504
1117	130-134	0.40388000926032674
1117	135-139	0.30543166245811193
1117	140-144	0.4471632958476306
1117	145-149	0.4904969448269014
1117	150-154	0.6417092419116379
1117	155-159	0.573188290633837
1117	160-164	0.792473748884106
1117	165-169	1.0387292316831314
1117	170-174	1.5509156018771257
1117	175-179	1.1723986463040745
1117	180-184	0.8233235351960744
1117	185-189	0.92681052291368
1117	190-194	0.8967931762062094
1117	195-199	0.844602147739252
1117	200-204	0.9242307822580251
1117	205-209	0.7685028572500272
1117	210-214	0.4575720678653319
1117	215-219	0.6318571607088046
1117	220-224	1.1268284897512864
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2108	200-204	-0.0031026112152865437
2108	205-209	-0.14804578471414231
2108	210-214	-0.07587335134788376
2108	215-219	-0.15667858123055467
2108	220-224	-0.49102225786378995
2108	225-229	-0.35774637667877585
2108	230-234	-0.174239504771311
2108	235-239	-0.2282639503192918
2108	240-244	-0.3731539109682416
2108	245-249	0.4467825578020843
2108	250-254	0.06211222380233394
2108	255-259	0.69848917022561
2108	260-264	0.07484143050667669
2108	265-269	-0.267791256966321
2108	270-274	-0.10873321233982658
2108	275-279	0.0498184893737843
2108	280-284	0.3293566018975138
2108	285-289	-0.1788393855518997
2108	290-294	0.021753391648704934
2108	295-299	1.1856796023171672
2108	300	1.2219045717322903
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	99.0
11	220.0
12	422.0
13	798.0
14	994.0
15	1633.0
16	5778.0
17	23982.0
18	37149.0
19	13671.0
20	5652.0
21	5496.0
22	7417.0
23	10754.0
24	15962.0
25	22524.0
26	29580.0
27	35949.0
28	42034.0
29	49033.0
30	56741.0
31	65086.0
32	68911.0
33	62338.0
34	40513.0
35	14413.0
36	1479.0
37	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.782654799737486	23.378130667692577	12.333060691389795	23.50615384118014
2	34.76508070955874	33.37040401656542	19.187149835606608	12.67736543826923
3	34.80856332782661	19.412806843420547	31.328014121343983	14.450615707408856
4	34.98168557379275	18.891177069627147	18.53329410737782	27.593843249202283
5	38.17030314982268	19.494922717324187	27.393726217917425	14.941047914935712
6	37.864470013157934	19.239846242875476	27.478266773137115	15.417416970829468
7	15.576961001425712	50.424642521151306	19.02324137864147	14.975155098781517
8	25.315935975481622	31.298756300130286	28.082497357097363	15.302810367290725
9	27.331816021647555	19.390661420733935	28.05340118130474	25.224121376313775
10-14	27.03946411310007	32.10032361413298	23.40480216216915	17.455410110597796
15-19	25.18128533973018	28.41254497783841	25.241870043547276	21.164299638884128
20-24	28.329976496755776	31.897587927026795	23.361578176574994	16.41085739964244
25-29	21.272214121990565	32.45028595074987	29.821187835212193	16.45631209204737
30-34	20.148487483795048	25.879852191426973	33.02529104258063	20.946369282197345
35-39	23.680892541356982	30.91481609600445	24.788616282866556	20.615675079772014
40-44	22.603946088019164	26.52216643659135	29.504233493577825	21.369653981811656
45-49	21.34401701802993	30.67687403618918	25.97661314047957	22.002495805301322
50-54	24.57631118683301	26.08953863163918	27.949592491893483	21.384557689634327
55-59	26.129432721559297	24.004765307013148	28.443516240515454	21.4222857309121
60-64	21.91801990825006	26.107869222388537	27.291275350043158	24.682835519318242
65-69	17.303754376549776	26.582524836818948	32.567284906520456	23.546435880110824
70-74	18.060610567084467	34.815578739101056	27.07518775115657	20.048622942657904
75-79	19.078653428984317	34.65066808052528	25.113555908301787	21.157122582188613
80-84	20.50000161645421	33.4804845483142	24.228450240690034	21.79106359454156
85-89	19.895480070736035	30.2377804143942	25.8619418787724	24.004797636097365
90-94	20.49437635580097	31.209883647625915	27.08181521342045	21.213924783152667
95-99	21.965640649297328	28.410087967438148	28.376465719855553	21.247805663408972
100-104	21.93327923599908	27.952178818630603	27.370966542630747	22.743575402739566
105-109	23.794496943285086	27.9871912168344	25.680026121900045	22.538285717980465
110-114	20.509894316223704	27.749119840682273	29.34155354181282	22.3994323012812
115-119	22.278359880899654	29.130379963726767	26.919878830592364	21.671381324781215
120-124	19.76742456816426	29.093072200543773	26.99730698728497	24.142196244006996
125-129	19.98331819254556	27.252124829059966	28.799330141375084	23.965226837019387
130-134	22.237948525632113	29.55220985455145	26.425599462044037	21.784242157772397
135-139	22.146133926464266	27.66512887989422	26.103601783272286	24.08513541036923
140-144	21.648628648297922	28.939356760935077	25.89069080486191	23.521323785905096
145-149	21.924693875703642	26.982614700432045	28.459278905776173	22.633412518088132
150-154	22.048112143127323	28.93032759061034	25.73954396593808	23.28201630032426
155-159	21.795040071899884	27.73334324758582	28.83072168214691	21.640894998367383
160-164	22.961577840862617	29.501979995402806	26.07082852033937	21.465613643395216
165-169	21.720295229197042	28.387522266656752	26.623550444686554	23.26863205945965
170-174	21.88483208909384	29.956310447344293	26.083736261746783	22.075121201815083
175-179	21.136801254886546	28.47867636884664	27.107211071554655	23.27731130471216
180-184	21.981521336070962	28.094726833698168	27.167011133170334	22.756740697060543
185-189	22.175689878633346	27.426613763336157	27.619683116679905	22.778013241350592
190-194	21.19680407398451	28.312666414068595	26.53825115059285	23.952278361354047
195-199	20.77618898289468	31.01956232885791	25.90943330348281	22.294815384764597
200-204	22.41240350977583	28.600030583323555	26.20428302311951	22.78328288378111
205-209	22.2254694991255	27.954959119873013	27.663706400188804	22.15586498081269
210-214	23.32573276317742	28.391119973593597	27.04610031912049	21.237046944108492
215-219	22.191796942183924	29.198192932340355	27.111640158955748	21.498369966519977
220-224	21.20513127224645	29.0988914357023	26.340347666971635	23.35562962507961
225-229	23.524111693178686	28.22547481756686	26.308488200523218	21.941925288731237
230-234	21.87198329232928	28.71291449927098	26.144433416634605	23.270668791765136
235-239	21.982167917964276	28.859406909826717	25.587411567802683	23.57101360440633
240-244	22.409745279145476	31.162262906578647	24.628716632343956	21.79927518193192
245-249	23.168670531069864	29.29438540794455	24.638900293871373	22.89804376711421
250-254	21.48753276957858	28.958010648557202	24.52313777140679	25.031318810457424
255-259	21.98509885923446	29.868847201558523	24.841344814052565	23.304709125154453
260-264	21.14161145665525	29.540160656203028	25.674538477926827	23.64368940921489
265-269	21.508107980539165	29.52611659107581	25.030268134451617	23.93550729393341
270-274	20.3423069201397	31.12396300387723	24.259590991691745	24.274139084291324
275-279	21.04345352209208	30.392572069610985	24.08177318561097	24.482201222685966
280-284	19.59934565933551	30.42590335543565	25.10107688179517	24.87367410343367
285-289	20.996576349981737	30.16183939557544	24.174428340968383	24.66715591347444
290-294	20.947371483807977	30.33816222087877	24.404482104235434	24.30998419107782
295-299	20.037385377157808	30.75383714148639	23.442254399021333	25.76652308233447
300	12.088006233047437	41.790514000109916	20.700959203928633	25.42052056291401
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.25
12	0.5
13	0.25
14	0.0
15	0.25
16	1.75
17	2.5
18	2.25
19	3.0
20	2.25
21	3.5
22	6.5
23	9.0
24	7.75
25	11.25
26	15.25
27	18.0
28	26.25
29	33.25
30	37.0
31	48.75
32	73.75
33	131.25
34	265.0
35	512.0
36	961.0
37	1928.75
38	3948.0
39	7084.75
40	9403.5
41	12167.25
42	16519.25
43	21048.0
44	24124.5
45	23918.5
46	22496.75
47	20121.75
48	15275.5
49	11273.0
50	7958.0
51	3918.25
52	1523.5
53	562.25
54	227.25
55	120.25
56	88.25
57	65.25
58	45.0
59	32.5
60	22.5
61	18.0
62	17.0
63	14.5
64	9.25
65	8.0
66	10.0
67	8.75
68	7.25
69	5.5
70	3.0
71	3.75
72	5.0
73	2.0
74	1.5
75	1.25
76	1.25
77	0.75
78	2.0
79	2.25
80	3.0
81	2.5
82	1.25
83	1.5
84	0.75
85	0.75
86	0.75
87	0.5
88	0.25
89	0.5
90	0.25
91	1.0
92	1.25
93	1.0
94	0.5
95	0.5
96	0.25
97	0.5
98	0.25
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	3.232908421403147E-5
145-149	6.465816842806294E-5
150-154	0.0
155-159	0.0
160-164	3.232908421403147E-5
165-169	0.0
170-174	6.465816842806294E-5
175-179	6.465816842806294E-5
180-184	3.232908421403147E-5
185-189	3.232908421403147E-5
190-194	6.465816842806294E-5
195-199	0.0
200-204	3.232908421403147E-5
205-209	0.0
210-214	3.232908421403147E-5
215-219	6.465816842806294E-5
220-224	0.0
225-229	6.465816842806294E-5
230-234	0.0
235-239	3.232908421403147E-5
240-244	0.0
245-249	0.0
250-254	3.232908421403147E-5
255-259	1.2931633685612587E-4
260-264	3.232908421403147E-5
265-269	9.698725264209442E-5
270-274	3.232908421403147E-5
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	6.465816842806294E-5
300	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	618638.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.04874532410196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.64573953937276	8.565945368616054
2	2.4782683289776153	0.6467658453923327
3	1.6700715707925609	0.6537701580088513
4	1.3852280865258693	0.7230195406747657
5	1.5055048735914156	0.982247483984435
6	1.5317736335340502	1.1992634422896065
7	1.625744267700376	1.4849746037938594
8	1.6940944487976635	1.768464561386848
9	1.640483856183255	1.9265630442872401
>10	20.159679532323754	49.193725370743635
>50	0.55889631777466	4.6276117624510675
>100	0.06605598753629924	1.5079156514034435
>500	0.003721893675340274	0.376386811876212
>1k	0.024812616581723877	9.246000256411966
>5k	0.007443784974508918	7.022809418412768
>10k+	0.0024812616581696392	10.074536680267014
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT	51761	8.366928640012414	Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	10471	1.6925892040256174	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	9448	1.5272259382708466	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	9176	1.4832583837397637	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	7886	1.274735790559261	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATACTCCTC	6566	1.0613638347466532	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	5248	0.8483151697761858	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	5057	0.8174408943517857	No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	4357	0.7042890996026755	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA	4322	0.69863150986522	No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	4313	0.6971767010755886	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	3954	0.6391459949114021	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG	3891	0.6289623333839822	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTCAAATT	3825	0.6182937355933518	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	3771	0.6095648828555633	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	3632	0.5870961693268115	No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	3541	0.5723864360094272	No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	3387	0.5474930411646229	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2885	0.46634703978740394	No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	2352	0.3801900303570101	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATAT	2328	0.37631054025132626	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	2125	0.34349651977408435	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATTT	1970	0.31844147950820995	Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGAACT	1546	0.24990382097446326	Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1293	0.20900752944371348	No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	1274	0.20593626644338048	No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	1208	0.19526766865275008	No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA	1140	0.18427578001997938	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCC	940	0.1519466958059479	Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCA	743	0.1201025478551269	Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGAGCT	642	0.10377636032704102	Illumina Single End PCR Primer 1 (96% over 32bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.008243916474578025	0.0	0.0
2	0.010668597790630384	0.0	0.0
3	0.016326187528085893	0.0	0.0
4	0.020367323054839827	0.0	0.0
5	0.026024912792295334	0.0	0.0
6	0.030712630003329897	0.0	0.0
7	0.040411355267539334	0.0	0.0
8	0.05221147100566082	0.0	0.0
9	0.06643626805983467	0.0	0.0
10-14	0.10645967431680563	0.0	0.0
15-19	0.1546300097957125	0.0	0.0
20-24	0.1987915388320795	0.0	0.0
25-29	0.23234912824624415	0.0	0.0
30-34	0.26807276630274895	0.0	0.0
35-39	0.28895735470501327	0.0	0.0
40-44	0.31501459658152264	0.0	0.0
45-49	0.3455979102479964	0.0	0.0
50-54	0.3752436804722632	0.0	0.0
55-59	0.4154933903187324	0.0	0.0
60-64	0.4617239807447975	0.0	0.0
65-69	0.5215004574565416	0.0	0.0
70-74	0.6075928087185074	0.0	0.0
75-79	0.7101406638454153	0.0	0.0
80-84	0.8419140111018073	0.0	0.0
85-89	1.029164066869478	0.0	0.0
90-94	1.279132546012369	0.0	0.0
95-99	1.5853859607718892	0.0	0.0
100-104	1.9854907070047425	0.0	0.0
105-109	2.4601139923509385	0.0	0.0
110-114	3.0269074967913383	0.0	0.0
115-119	3.7066264923913494	0.0	0.0
120-124	4.468752323652928	0.0	1.6164542107015734E-4
125-129	5.307465755417546	0.0	1.6164542107015734E-4
130-134	6.239707227813358	0.0	1.6164542107015734E-4
135-139	7.243202001816894	0.0	1.6164542107015734E-4
140-144	8.317885419259728	0.0	1.6164542107015734E-4
145-149	9.45751796688855	0.0	1.6164542107015734E-4
150-154	10.623498718151811	0.0	1.6164542107015734E-4
155-159	11.78178514737213	0.0	1.6164542107015734E-4
160-164	12.88866833269214	0.0	1.6164542107015734E-4
165-169	14.006317103055423	0.0	1.6164542107015734E-4
170-174	15.189981863383755	0.0	1.6164542107015734E-4
175-179	16.43209760797106	0.0	1.6164542107015734E-4
180-184	17.694289713855277	0.0	1.6164542107015734E-4
185-189	18.94089920114833	0.0	1.6164542107015734E-4
190-194	20.20234773809562	0.0	1.6164542107015734E-4
195-199	21.448601605462322	0.0	1.6164542107015734E-4
200-204	22.670220710657926	0.0	1.6164542107015734E-4
205-209	23.892227764864106	0.0	1.6164542107015734E-4
210-214	25.070816858970836	0.0	1.6164542107015734E-4
215-219	26.21856400673738	0.0	1.6164542107015734E-4
220-224	27.329294353078858	0.0	1.6164542107015734E-4
225-229	28.413611837617474	0.0	1.6164542107015734E-4
230-234	29.446041141992573	6.465816842806294E-5	1.6164542107015734E-4
235-239	30.439998836152967	1.6164542107015734E-4	1.939745052841888E-4
240-244	31.345698130409062	1.6164542107015734E-4	3.232908421403147E-4
245-249	32.17978850313108	1.6164542107015734E-4	3.232908421403147E-4
250-254	32.96160274667899	1.6164542107015734E-4	3.232908421403147E-4
255-259	33.7131892964868	1.6164542107015734E-4	3.232908421403147E-4
260-264	34.442274803681634	1.6164542107015734E-4	3.232908421403147E-4
265-269	35.11614223503891	3.232908421403147E-4	3.232908421403147E-4
270-274	35.68439054826894	3.232908421403147E-4	3.232908421403147E-4
275-279	36.166869801079145	3.232908421403147E-4	3.232908421403147E-4
280-284	36.614853921032974	3.232908421403147E-4	3.232908421403147E-4
285-288	36.99602998845852	3.232908421403147E-4	3.232908421403147E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	5210	0.0	289.2029	1
GGGTTAT	5225	0.0	288.0913	2
GGGGGGA	6485	0.0	283.799	1
GGGGGAG	6845	0.0	271.66492	2
GGTTATT	5570	0.0	269.9833	3
ATTAGTA	5655	0.0	265.66528	7
GGAGCGA	5140	0.0	255.39056	5
GTTATTA	5875	0.0	254.71611	4
GAGCGAA	5205	0.0	252.48366	6
TATTAGT	5965	0.0	251.11938	6
TTATTAG	6005	0.0	248.95705	5
TTAGTAG	6085	0.0	247.37506	8
GCGAAAG	5335	0.0	245.50467	8
CGAAAGC	5330	0.0	245.18341	9
GGGGAGC	7700	0.0	240.16318	3
TAGTAGA	6300	0.0	238.4662	9
AGCGAAA	5800	0.0	226.58232	7
GGGAGCG	5830	0.0	225.41638	4
GAGCAAA	3410	0.0	155.6214	6
GCAAAAG	3455	0.0	152.74356	8
>>END_MODULE