##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE77A l01n02 clinical flu pool a4.3420000000b72d.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	545627
Sequences flagged as poor quality	0
Sequence length	300
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90992931068294	34.0	34.0	34.0	33.0	34.0
2	33.303670822741545	34.0	34.0	34.0	34.0	34.0
3	33.33834286059891	34.0	34.0	34.0	34.0	34.0
4	33.33720105493313	34.0	34.0	34.0	34.0	34.0
5	33.338509641201775	34.0	34.0	34.0	34.0	34.0
6	36.839428767271414	38.0	38.0	38.0	36.0	38.0
7	36.835334395108745	38.0	38.0	38.0	36.0	38.0
8	36.79477555179638	38.0	38.0	38.0	36.0	38.0
9	36.77453461797162	38.0	38.0	38.0	36.0	38.0
10-14	36.887821533758405	38.0	38.0	38.0	36.0	38.0
15-19	36.874843803550775	38.0	38.0	38.0	36.0	38.0
20-24	36.856750857270626	38.0	38.0	38.0	36.0	38.0
25-29	36.78056327857676	38.0	38.0	38.0	36.0	38.0
30-34	36.857419445885185	38.0	38.0	38.0	36.0	38.0
35-39	36.781933445375685	38.0	38.0	38.0	35.8	38.0
40-44	36.66270474151756	38.0	38.0	38.0	35.0	38.0
45-49	36.71458890414148	38.0	38.0	38.0	35.0	38.0
50-54	36.58814208241161	38.0	38.0	38.0	34.4	38.0
55-59	36.60277185696456	38.0	38.0	38.0	34.6	38.0
60-64	36.55345318321857	38.0	38.0	38.0	34.4	38.0
65-69	36.224622315244666	38.0	37.0	38.0	33.8	38.0
70-74	36.45465931854545	38.0	37.0	38.0	34.0	38.0
75-79	36.32719898392126	38.0	37.4	38.0	33.4	38.0
80-84	35.56308870345492	38.0	37.0	38.0	31.0	38.0
85-89	35.6069849915785	38.0	37.0	38.0	31.0	38.0
90-94	35.6110998905846	38.0	37.0	38.0	30.8	38.0
95-99	35.45993325110378	38.0	37.0	38.0	29.8	38.0
100-104	35.46593002179144	38.0	37.0	38.0	29.6	38.0
105-109	35.27558716852356	38.0	37.0	38.0	27.6	38.0
110-114	35.15827772452609	38.0	37.0	38.0	27.0	38.0
115-119	35.20288622080652	38.0	37.0	38.0	27.0	38.0
120-124	34.89314458412066	38.0	36.8	38.0	25.2	38.0
125-129	34.79671937055901	38.0	36.2	38.0	24.4	38.0
130-134	34.82976905468388	38.0	36.2	38.0	24.8	38.0
135-139	34.536922476343726	38.0	36.0	38.0	24.0	38.0
140-144	34.43951893876219	38.0	35.8	38.0	24.0	38.0
145-149	34.191651072985756	38.0	35.0	38.0	23.0	38.0
150-154	34.02741066699412	38.0	35.0	38.0	23.0	38.0
155-159	33.778461109879096	37.8	34.2	38.0	22.4	38.0
160-164	33.50862475647283	37.0	34.0	38.0	21.2	38.0
165-169	32.7606474752899	37.0	32.2	38.0	18.0	38.0
170-174	32.19842676407143	36.8	29.2	38.0	17.0	38.0
175-179	32.52675142542433	37.0	31.0	38.0	17.2	38.0
180-184	32.541871278364155	37.0	30.8	38.0	17.0	38.0
185-189	32.25400209300493	37.0	30.0	38.0	16.8	38.0
190-194	31.487358946679688	37.0	28.0	37.8	12.8	38.0
195-199	31.14418751271473	36.0	28.0	37.8	10.4	38.0
200-204	30.45821816002507	35.0	25.4	37.0	10.2	37.8
205-209	29.83430988569114	35.0	24.0	37.0	10.0	37.6
210-214	29.151697038453005	34.2	22.8	37.0	10.0	37.2
215-219	28.332221829198335	33.0	21.4	37.0	9.8	37.0
220-224	27.205886438904237	31.2	20.0	36.8	9.2	37.0
225-229	26.509488716650754	29.8	19.4	36.2	9.0	37.0
230-234	25.535697463651907	28.4	17.0	35.4	9.0	37.0
235-239	24.458057244234617	26.4	15.2	34.0	8.0	37.0
240-244	23.725265062029557	25.0	14.4	33.6	8.0	37.0
245-249	22.614767597644544	23.2	12.2	32.4	8.0	36.4
250-254	21.336577552063954	21.4	10.4	30.8	8.0	36.0
255-259	20.37465338042289	19.8	9.0	29.4	7.8	35.8
260-264	19.88719473193225	19.2	9.0	28.8	7.4	35.2
265-269	19.0479642686304	18.0	8.8	27.6	7.4	34.2
270-274	18.03962927054563	16.2	8.0	26.0	7.0	33.2
275-279	16.98475295394106	14.6	8.0	23.8	7.0	31.4
280-284	16.00829284474559	13.2	8.0	22.2	7.0	30.2
285-289	15.663747211923162	13.0	8.0	21.4	7.0	29.6
290-294	15.181189347301364	13.0	8.0	21.0	7.0	28.8
295-299	14.511201608424802	12.0	8.0	19.6	7.0	27.2
300	12.469161166877738	11.0	8.0	15.0	7.0	22.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.014886186229915666
1101	2	-0.036329082085480024
1101	3	0.011927612980613844
1101	4	-0.09576202200246797
1101	5	-0.01331911784409101
1101	6	0.041185426742842424
1101	7	-0.21314207387458595
1101	8	-0.12281411644903528
1101	9	-0.14170334623415215
1101	10-14	-0.018335822481908792
1101	15-19	0.04973107517639619
1101	20-24	-0.10332331820305285
1101	25-29	-0.06780225204501278
1101	30-34	-0.007317304956728776
1101	35-39	-0.006910137070661904
1101	40-44	0.011987493686866912
1101	45-49	0.0015510021571074617
1101	50-54	0.012676046859404266
1101	55-59	-0.0018557560850211985
1101	60-64	0.04009462427816146
1101	65-69	0.04192201041061594
1101	70-74	0.05688455775744927
1101	75-79	0.18487621605387972
1101	80-84	-0.003925619101160294
1101	85-89	0.017021652582862146
1101	90-94	-0.061929948516791455
1101	95-99	-0.11458670071630195
1101	100-104	-0.07793789726022737
1101	105-109	0.059081048710673656
1101	110-114	0.19867567458724977
1101	115-119	0.1789505662137998
1101	120-124	0.03557716753356033
1101	125-129	0.020285973769958332
1101	130-134	-0.014405148982007177
1101	135-139	-0.1483313876052108
1101	140-144	-0.061640970541645856
1101	145-149	0.09292258561734457
1101	150-154	-0.08053108677654564
1101	155-159	0.10748349415966629
1101	160-164	-0.0021268765145450175
1101	165-169	0.3800246963226783
1101	170-174	1.1211879669836122
1101	175-179	0.2619086798356989
1101	180-184	-0.2334362123850653
1101	185-189	-0.1632061479325273
1101	190-194	-0.7600584038947922
1101	195-199	-0.5938727421381742
1101	200-204	-0.4447748435493786
1101	205-209	-0.2825745739614618
1101	210-214	-0.8858317504884425
1101	215-219	-0.643429928661039
1101	220-224	-0.7646323961771557
1101	225-229	-0.46024196256735195
1101	230-234	-0.17521005643247278
1101	235-239	-0.31534630015471166
1101	240-244	-0.3020683185617443
1101	245-249	-0.20253032987021768
1101	250-254	0.13935379172233908
1101	255-259	0.12651141846275848
1101	260-264	0.1371256499573299
1101	265-269	-0.1487282276380668
1101	270-274	-0.002188752048699172
1101	275-279	0.08984320835049786
1101	280-284	0.38093373553506815
1101	285-289	0.14590153505501036
1101	290-294	-0.011556157781699383
1101	295-299	-0.10264880835615209
1101	300	0.6380220767059246
1102	1	0.11577038744077583
1102	2	0.14654035330299564
1102	3	0.0679001036762088
1102	4	0.10222662157389095
1102	5	0.11171108541028474
1102	6	0.2697032493677014
1102	7	0.14394153594624726
1102	8	0.0408401888220169
1102	9	0.14821769254141515
1102	10-14	0.13303973289733761
1102	15-19	0.07514652770228736
1102	20-24	0.07230932727745909
1102	25-29	0.04160186930174348
1102	30-34	0.11199086921596546
1102	35-39	0.013833367877261082
1102	40-44	0.14661566006983406
1102	45-49	0.016601599370112297
1102	50-54	-0.031634651764406385
1102	55-59	0.12637995912804456
1102	60-64	0.13528734808878795
1102	65-69	0.2567525295820161
1102	70-74	0.048030856912113506
1102	75-79	0.22879201839656815
1102	80-84	0.21158046121965413
1102	85-89	0.06074676870760953
1102	90-94	0.11683833572197955
1102	95-99	0.2101138813500043
1102	100-104	0.07199818003125813
1102	105-109	0.26645244595830775
1102	110-114	0.1005496287432095
1102	115-119	0.17444928956282268
1102	120-124	-0.0012292395233615139
1102	125-129	0.18610481555179348
1102	130-134	0.20058116488660716
1102	135-139	0.17004715744540988
1102	140-144	0.11893292735511807
1102	145-149	0.27162279983504334
1102	150-154	0.21066561124138872
1102	155-159	0.31312960939004597
1102	160-164	0.2613155551810209
1102	165-169	0.46475870933441854
1102	170-174	1.32613787466083
1102	175-179	0.6165693575426303
1102	180-184	0.5100169842913189
1102	185-189	0.43657665911285193
1102	190-194	0.177906545904694
1102	195-199	0.05124307607249534
1102	200-204	0.3915487622323326
1102	205-209	0.08560571151789276
1102	210-214	0.5828492779008165
1102	215-219	0.4694697460454016
1102	220-224	0.31062608958493243
1102	225-229	0.5259464949208912
1102	230-234	0.03404007339644366
1102	235-239	0.20956940120993295
1102	240-244	0.4428078571112053
1102	245-249	0.7666840789754623
1102	250-254	0.9682395530967902
1102	255-259	1.2478143598089417
1102	260-264	0.7554277402028511
1102	265-269	0.46835933114557804
1102	270-274	0.8580949601927266
1102	275-279	1.0689877259639537
1102	280-284	1.2014307458636768
1102	285-289	0.7871915476051399
1102	290-294	0.7265098602212436
1102	295-299	0.5567751479636289
1102	300	0.7836166390382289
1103	1	-0.14888442759190212
1103	2	-0.05110201088280775
1103	3	-0.04697013478044454
1103	4	-0.05684522848725493
1103	5	-0.14497728073239813
1103	6	0.07850937821034876
1103	7	0.03405371130556034
1103	8	-0.05981765219815571
1103	9	-0.10456458747790975
1103	10-14	-0.05906852058731005
1103	15-19	-0.16323777180732435
1103	20-24	-0.15815246060839883
1103	25-29	-0.1510706216841271
1103	30-34	-0.19015497453968777
1103	35-39	-0.171444670662126
1103	40-44	-0.18333706000331773
1103	45-49	-0.3138084471970757
1103	50-54	-0.35999606690526065
1103	55-59	-0.19684169806474472
1103	60-64	-0.2646204468062834
1103	65-69	-0.15818578006022221
1103	70-74	-0.20925621087081225
1103	75-79	-0.041158184531347786
1103	80-84	0.15373646377649663
1103	85-89	0.06064418827286744
1103	90-94	-0.1376064330141702
1103	95-99	-0.0914056533660883
1103	100-104	-0.09226350022183283
1103	105-109	-0.06418026420866596
1103	110-114	-0.14265333481186104
1103	115-119	-0.22448376565153438
1103	120-124	-0.11161002766047545
1103	125-129	-0.05682559260014841
1103	130-134	0.07802984421989123
1103	135-139	-0.034989568966601325
1103	140-144	0.02328231072220177
1103	145-149	0.03624391760090617
1103	150-154	-0.12315385892209463
1103	155-159	-0.075057220527178
1103	160-164	0.15291230490596064
1103	165-169	0.4923818069838646
1103	170-174	1.3609226564879506
1103	175-179	0.7244147759548696
1103	180-184	0.32323497403056933
1103	185-189	0.2631884778765965
1103	190-194	0.242984945054463
1103	195-199	0.1566917030780388
1103	200-204	0.20534966506646768
1103	205-209	0.4613421456613942
1103	210-214	0.39333106761814207
1103	215-219	0.2319174577664107
1103	220-224	0.043121036122670375
1103	225-229	0.29312725166136033
1103	230-234	0.30129494607987084
1103	235-239	0.12660285082214884
1103	240-244	0.39772524467533543
1103	245-249	0.5479988317471403
1103	250-254	0.5951306035628505
1103	255-259	0.9065754461871691
1103	260-264	0.8857362889784035
1103	265-269	0.3996538069139959
1103	270-274	0.3785037301432439
1103	275-279	0.8859591491479435
1103	280-284	1.0794849835769202
1103	285-289	0.5600449933649436
1103	290-294	0.7823087448281818
1103	295-299	0.5362294687697275
1103	300	0.83525600881363
2116	1	0.20550560981859434
2116	2	0.0723051075023804
2116	3	0.12686823639749178
2116	4	0.04283005640144211
2116	5	-0.032304072548029694
2116	6	0.08898934991833585
2116	7	-0.025256535834834892
2116	8	-0.09356025361862663
2116	9	0.055146882481167836
2116	10-14	0.1109778266821948
2116	15-19	0.13458147896695039
2116	20-24	-0.023609603478853103
2116	25-29	0.1122244562423731
2116	30-34	0.09740870228467458
2116	35-39	0.056699262607494916
2116	40-44	0.13646217482642697
2116	45-49	0.0922852379350303
2116	50-54	0.18155279016184522
2116	55-59	0.08977127031780441
2116	60-64	-0.02561223271143831
2116	65-69	0.22625286909882192
2116	70-74	0.05591819827954936
2116	75-79	0.15207577575826292
2116	80-84	0.23142188663533858
2116	85-89	0.322980357175048
2116	90-94	0.1733261083416906
2116	95-99	0.15963617007071917
2116	100-104	0.2885127501456779
2116	105-109	0.29861080405233054
2116	110-114	0.35766495029723444
2116	115-119	0.22879653724816507
2116	120-124	0.37782477980521634
2116	125-129	0.36928906924929095
2116	130-134	0.2137198510179985
2116	135-139	0.20947231206996264
2116	140-144	0.32944052884744934
2116	145-149	0.3235831966671938
2116	150-154	0.47938990586283836
2116	155-159	0.31699268747253484
2116	160-164	0.41282464254258144
2116	165-169	0.726155626530975
2116	170-174	1.4374638185198165
2116	175-179	1.1985118848301823
2116	180-184	0.5756269115533073
2116	185-189	0.9052958598452889
2116	190-194	0.76331237194929
2116	195-199	0.4767536361137559
2116	200-204	0.8697768893230489
2116	205-209	0.6895429238780366
2116	210-214	0.9559986611991356
2116	215-219	1.297123923739349
2116	220-224	1.390178354932896
2116	225-229	0.809586333317263
2116	230-234	1.0682479031586674
2116	235-239	1.0036349295440097
2116	240-244	1.1238071507889167
2116	245-249	1.0552963717121635
2116	250-254	1.3796690575387132
2116	255-259	1.6414973006929934
2116	260-264	1.2597310303548603
2116	265-269	1.1196128599144544
2116	270-274	1.3028025686891525
2116	275-279	1.185610950550334
2116	280-284	1.2735968721384534
2116	285-289	1.0213404337944674
2116	290-294	0.7708143237869756
2116	295-299	0.7636084181539626
2116	300	0.9626995843794344
1117	1	0.06814847886996489
1117	2	7.302598651648395E-4
1117	3	-0.07448543049764567
1117	4	0.0461482306414851
1117	5	0.12746011247318023
1117	6	0.14846362885800346
1117	7	0.2436146828821677
1117	8	0.11479237591289149
1117	9	-0.010655314506770708
1117	10-14	0.16094957704805068
1117	15-19	0.045283366598248165
1117	20-24	0.13223705194970847
1117	25-29	0.13253705465298538
1117	30-34	0.18297573264608502
1117	35-39	0.07028066786470788
1117	40-44	0.12210867465044117
1117	45-49	0.12205775891386139
1117	50-54	0.09673377306386755
1117	55-59	0.10516759879159565
1117	60-64	0.0960993246306856
1117	65-69	0.4191304416679671
1117	70-74	0.26982007479622183
1117	75-79	0.3302632607072269
1117	80-84	0.38555587326075624
1117	85-89	0.17029867960988554
1117	90-94	0.2395300984867319
1117	95-99	0.3786602146773461
1117	100-104	0.4106997349371895
1117	105-109	0.36390478549093785
1117	110-114	0.443243095268798
1117	115-119	0.41260949688361137
1117	120-124	0.5205088655359162
1117	125-129	0.4120757798804249
1117	130-134	0.41939552669366975
1117	135-139	0.38490744905752905
1117	140-144	0.36648256059818607
1117	145-149	0.5165675621155756
1117	150-154	0.3644586312022966
1117	155-159	0.5809558795885792
1117	160-164	0.7357777944607733
1117	165-169	1.1498770758761339
1117	170-174	1.5974056520599973
1117	175-179	1.087603450693507
1117	180-184	0.8308161377911958
1117	185-189	0.8944884760392711
1117	190-194	1.091959075541169
1117	195-199	0.7770445610345647
1117	200-204	1.0170729825375773
1117	205-209	1.0051200500103334
1117	210-214	0.7148520039181392
1117	215-219	1.1382673392590519
1117	220-224	1.3161497718604416
1117	225-229	1.269319582673539
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2108	200-204	0.23148810379292328
2108	205-209	-0.6044685685654443
2108	210-214	0.06908649518543797
2108	215-219	-0.6822884304907291
2108	220-224	-0.6195746758397505
2108	225-229	-0.7043018847282383
2108	230-234	-0.5257663269776529
2108	235-239	-0.7010067309058101
2108	240-244	-0.8837065450084332
2108	245-249	-0.238059736618343
2108	250-254	-0.44185996347210477
2108	255-259	0.15646358307232333
2108	260-264	-0.29437399340929105
2108	265-269	-0.6442360037963475
2108	270-274	-0.6146674315748406
2108	275-279	-0.2941379443506129
2108	280-284	0.07281819407256229
2108	285-289	-0.5866577358623122
2108	290-294	-0.38354686980799535
2108	295-299	0.9222414881147252
2108	300	1.6260234954865478
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	62.0
11	404.0
12	1000.0
13	1024.0
14	895.0
15	832.0
16	1113.0
17	2459.0
18	9662.0
19	10535.0
20	5871.0
21	4531.0
22	5063.0
23	6360.0
24	8482.0
25	11069.0
26	14800.0
27	19927.0
28	26380.0
29	35746.0
30	48220.0
31	62980.0
32	77584.0
33	82702.0
34	67576.0
35	33677.0
36	6595.0
37	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.526150648703236	30.986003258636398	12.826528012726643	21.66131807993373
2	27.61208664527232	28.86990563150284	23.81278785690591	19.70521986631893
3	33.512637754363325	23.900026941482004	27.82175368887537	14.765581615279302
4	34.74168250471476	21.836895901412504	22.79066101934105	20.63076057453169
5	37.11179981929047	23.51533190256347	23.009858383107872	16.363009895038186
6	30.135422184019482	24.09631488177821	29.686397483995474	16.081865450206827
7	16.58294036035607	38.08334264983221	29.981104307521438	15.352612682290282
8	25.62776402194173	32.509388281738254	24.461032903430365	17.401814792889645
9	19.576743819495736	23.991298084588923	29.67338493146417	26.75857316445117
10-14	23.14837792118059	32.857684828646676	26.83063704692033	17.163300203252405
15-19	23.759857924919405	29.136901216398748	26.020926383774995	21.08231447490685
20-24	21.180989943679474	29.000397707591453	30.22859939115916	19.590012957569915
25-29	18.957896145168768	29.645013901438162	32.12121101045219	19.275878942940874
30-34	19.36993587194182	30.002987388820568	31.03255520712868	19.59452153210893
35-39	21.379990359714604	30.73975444763547	28.721269292025504	19.15898590062442
40-44	21.34626768836586	28.80114070601345	30.78894556171157	19.06364604390912
45-49	20.792995947781176	30.495155115124433	29.29360167293774	19.418247264156648
50-54	20.114070601344874	29.7162347171236	31.181704717691755	18.987989963839766
55-59	22.097146952038663	29.514521825349554	27.98736132925973	20.400969893352052
60-64	19.197217146512177	28.850954956407946	29.698713590053277	22.253114307026596
65-69	18.177729474531134	29.361047015635222	30.988935664840632	21.47228784499301
70-74	18.496921889862488	33.436651778596	27.356124238719858	20.710302092821653
75-79	18.420532708242078	35.31636081059039	27.214159123357167	19.048947357810373
80-84	20.76733739349409	32.156693125523475	27.092317645571057	19.98365183541137
85-89	21.188944095508468	30.76079446215088	28.130206166483696	19.92005527585695
90-94	19.489541389997196	30.879886809120517	28.45852569612574	21.172046104756546
95-99	19.45076031794614	29.757544989525815	29.40532634931922	21.386368343208822
100-104	19.812106072463422	29.253317742707015	28.757301233260087	22.17727495156948
105-109	20.453863170261002	29.816486354231003	29.230921490322142	20.498728985185853
110-114	19.551964987069923	28.967738033491745	30.350880729875907	21.12941624956243
115-119	18.490030735282527	29.039472020262924	30.462348820714517	22.008148423740028
120-124	19.780179499914777	28.942995856143483	29.774699565820605	21.502125078121136
125-129	20.925540708212754	29.592157279606763	29.75314638021945	19.729155631961028
130-134	21.361699476015666	28.367584448716798	30.022267959613437	20.2484481156541
135-139	20.980999840550414	28.69956948611414	29.63859926286639	20.680831410469054
140-144	18.971531834018478	28.542319203411854	31.4454013456079	21.04074761696177
145-149	19.651373557393605	30.23966922458016	29.136351390235454	20.97260582779078
150-154	20.60041017031782	27.28153115589954	30.370271265901433	21.74778740788121
155-159	19.806461190520263	28.728930203234075	31.046337516288602	20.418271089957056
160-164	22.263927554904726	29.01260384841659	28.283900906663344	20.43956769001534
165-169	20.628414649568295	28.300542311872395	29.627969290376026	21.443073748183284
170-174	19.620143431318464	30.093488775298876	31.125622449035696	19.160745344346964
175-179	19.69759561018791	30.91789812454296	28.688536307770697	20.695969957498438
180-184	21.92083602900883	28.664343956585725	29.199984604867428	20.21483540953802
185-189	19.910195059995196	30.42708663610855	28.102128377078113	21.56058992681814
190-194	21.126887049211273	29.477646817331255	28.663720820267326	20.731745313190146
195-199	20.75010950704419	30.902502991970703	29.37024744010102	18.977140060884086
200-204	21.393882634107182	28.954468895417566	28.528316963786615	21.123331506688636
205-209	22.505851066754396	29.190454284703655	28.75634820124371	19.547346447298246
210-214	22.751256810971597	28.64524666118062	28.796888717017303	19.806607810830474
215-219	21.775938507441897	29.084741041040857	28.946441433433463	20.192879018083783
220-224	23.17964470233328	28.31993284789792	29.231178075865017	19.269244373903785
225-229	21.83722579711048	29.65406770559375	28.93067975008568	19.57802674721009
230-234	21.688736077943357	29.622287753355316	27.261187587857638	21.42778858084369
235-239	23.36343326118392	30.012077848053707	26.342464724069742	20.28202416669263
240-244	21.954485390202464	30.214743771844134	28.08237129027706	19.748399547676343
245-249	24.60816638472803	28.441334464753393	27.496549840825324	19.453949309693254
250-254	23.90394903478017	30.373386947493437	26.313213972182464	19.409450045543934
255-259	23.262741763145886	29.751936762660208	27.33819990579645	19.64712156839746
260-264	22.7365214697953	30.403883972017514	27.00885403398292	19.850740524204262
265-269	23.283745122583742	30.741513891358014	26.957756855874067	19.016984130184174
270-274	24.365106565474214	29.228612220436307	27.4784055774366	18.927875636652878
275-279	23.29331209782507	30.269433147553183	25.90425327192386	20.533001482697887
280-284	23.66825688611451	29.338944003870775	26.762421947594234	20.23037716242048
285-289	24.19161808341596	30.61413749686141	25.66588530259683	19.528359117125802
290-294	22.891755723232173	31.760525047330866	24.830406119931748	20.51731310950521
295-299	23.742593383391952	31.273305756496654	25.196590344686022	19.78751051542537
300	15.484754236868778	42.883874881558285	25.58524413198027	16.04612674959267
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.25
11	0.5
12	1.75
13	1.5
14	2.0
15	2.25
16	3.25
17	4.5
18	6.25
19	8.0
20	13.25
21	14.5
22	22.0
23	31.0
24	38.75
25	45.75
26	58.5
27	79.0
28	92.0
29	114.0
30	134.5
31	211.0
32	438.25
33	1218.0
34	3212.25
35	4390.25
36	5990.25
37	7708.25
38	9819.5
39	11382.75
40	14857.25
41	18963.0
42	21226.5
43	19893.5
44	19481.5
45	16817.5
46	11208.0
47	6370.75
48	3688.5
49	1872.0
50	858.25
51	391.5
52	233.5
53	168.75
54	136.25
55	111.25
56	98.0
57	63.0
58	51.75
59	33.0
60	25.25
61	23.75
62	22.5
63	16.5
64	13.5
65	12.25
66	10.25
67	13.25
68	10.75
69	6.0
70	5.75
71	4.75
72	4.25
73	3.0
74	4.0
75	2.25
76	2.5
77	2.75
78	4.75
79	2.75
80	2.0
81	3.0
82	1.0
83	1.25
84	2.5
85	2.5
86	2.25
87	1.5
88	1.25
89	1.25
90	0.25
91	0.5
92	1.0
93	0.5
94	0.75
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.3333333333333333
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	545627.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.329100404437021
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.15382043890716	10.753949572628539
2	2.5081096963463687	0.7689413072127108
3	1.47012447736732	0.6760705716175246
4	1.4910350797227971	0.9142490577443407
5	1.2736005367450889	0.9761575251455176
6	1.3388607211538053	1.2314118253274184
7	1.406987964105503	1.5097501838725385
8	1.3391430040325074	1.6422286051770973
9	1.3013632504704298	1.7953855136195171
>10	16.970011099992686	54.58323899782035
>50	0.6742375156991164	6.2171102921991155
>100	0.03941158442959939	1.265368448099183
>500	0.007134574301935818	0.7830645364373534
>1k	0.019025495800509596	7.890603537723225
>5k	0.005945467437596111	5.523185515849239
>10k+	0.0011890934875192223	3.4692845095263705
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT	19033	3.4882804553293734	Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA	6696	1.2272119964737815	No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	6237	1.1430885934896917	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT	5892	1.0798585847108007	No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	5825	1.0675791337305522	No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	5651	1.0356892162594593	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT	4350	0.7972479367773223	No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	3887	0.7123914322421727	No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	3596	0.6590582944025863	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC	3442	0.6308338846867916	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG	3349	0.6137892736246556	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA	3305	0.605725156563	No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	3301	0.6049920550119404	No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT	3231	0.5921627778683973	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG	2915	0.5342477553346883	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA	2625	0.4810978928828669	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	2199	0.40302257769501876	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT	1706	0.31266781152692225	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT	1655	0.30332076675091224	No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT	1572	0.28810890956642543	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG	1146	0.2100335943785773	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT	1010	0.18510814164255068	No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT	1000	0.18327538776490168	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT	809	0.14826978870180546	No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT	711	0.13030880070084508	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG	661	0.12114503131259999	No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA	594	0.10886558033235158	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.004032058530827836	0.0	0.0
2	0.004581884694122541	0.0	0.0
3	0.00788084167389077	0.0	0.0
4	0.010080146327069592	0.0	0.0
5	0.011912900204718609	0.0	0.0
6	0.015578407960016642	0.0	0.0
7	0.01851081416425507	0.0	0.0
8	0.025841829674851133	0.0	0.0
9	0.038121280655099544	0.0	0.0
10-14	0.07536283944892756	0.0	0.0
15-19	0.13676009435016961	0.0	0.0
20-24	0.1877473072263653	0.0	0.0
25-29	0.22520879648551118	0.0	0.0
30-34	0.25691543856883914	0.0	0.0
35-39	0.2803746882027466	0.0	0.0
40-44	0.3026609753549586	0.0	0.0
45-49	0.3344042725158396	0.0	0.0
50-54	0.3668073610726742	0.0	0.0
55-59	0.4023627862990651	0.0	0.0
60-64	0.4493912507995389	0.0	0.0
65-69	0.4926442423120556	0.0	0.0
70-74	0.5412488751473077	0.0	0.0
75-79	0.6002635500076059	0.0	0.0
80-84	0.6777890390321594	0.0	0.0
85-89	0.7574405225547856	0.0	1.4662031021192134E-4
90-94	0.8541366171395477	0.0	1.8327538776490167E-4
95-99	0.9632954380923232	0.0	1.8327538776490167E-4
100-104	1.0965733000749596	0.0	1.8327538776490167E-4
105-109	1.2510377968832187	0.0	1.8327538776490167E-4
110-114	1.4197611188595871	0.0	1.8327538776490167E-4
115-119	1.6036962980204426	0.0	1.8327538776490167E-4
120-124	1.8159658521297515	0.0	1.8327538776490167E-4
125-129	2.069142472788187	0.0	1.8327538776490167E-4
130-134	2.361686646738523	0.0	1.8327538776490167E-4
135-139	2.699316566079025	0.0	1.8327538776490167E-4
140-144	3.064437793584262	0.0	1.8327538776490167E-4
145-149	3.467313750969068	0.0	1.8327538776490167E-4
150-154	3.934152818683826	0.0	1.8327538776490167E-4
155-159	4.491053411946256	0.0	1.8327538776490167E-4
160-164	5.033878455428342	0.0	1.8327538776490167E-4
165-169	5.625161511435468	0.0	1.8327538776490167E-4
170-174	6.333190989448837	0.0	1.8327538776490167E-4
175-179	7.136890219875482	0.0	1.8327538776490167E-4
180-184	8.010747268738534	0.0	1.8327538776490167E-4
185-189	8.964475731589529	0.0	1.8327538776490167E-4
190-194	10.001227945098027	0.0	1.8327538776490167E-4
195-199	11.125035967794847	0.0	1.8327538776490167E-4
200-204	12.31903846400563	0.0	2.5658554287086234E-4
205-209	13.618497618336338	0.0	3.6655077552980335E-4
210-214	14.978657581094776	0.0	3.6655077552980335E-4
215-219	16.3459286288985	0.0	3.6655077552980335E-4
220-224	17.709754099412237	0.0	3.6655077552980335E-4
225-229	19.106055968637918	0.0	3.6655077552980335E-4
230-234	20.46702234310252	0.0	3.6655077552980335E-4
235-239	21.834953182302193	0.0	3.6655077552980335E-4
240-244	23.158934583515848	0.0	3.6655077552980335E-4
245-249	24.439956233837403	0.0	3.6655077552980335E-4
250-254	25.63194270078277	0.0	3.6655077552980335E-4
255-259	26.787017504632285	0.0	3.6655077552980335E-4
260-264	27.876736305204837	0.0	3.6655077552980335E-4
265-269	28.891422162026437	0.0	3.6655077552980335E-4
270-274	29.80831227193669	0.0	3.6655077552980335E-4
275-279	30.681729459869103	0.0	3.6655077552980335E-4
280-284	31.455408181779866	0.0	3.6655077552980335E-4
285-288	32.10526605171665	0.0	3.6655077552980335E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTT	3590	0.0	281.30643	1
CGGGTTA	3695	0.0	273.31256	2
GGGGGGA	5925	0.0	263.73163	1
GGGGAGC	6085	0.0	257.52176	3
CAGAAGC	6105	0.0	255.95576	9
GGAGCAG	6140	0.0	255.21498	5
GAGCAGA	6280	0.0	248.82324	6
GGGTTAT	4175	0.0	242.24191	3
GGGAGCA	6755	0.0	232.19688	4
TATTAGT	4360	0.0	229.94035	7
TTATTAG	4415	0.0	226.74294	6
ATTAGTA	4440	0.0	225.7973	8
GGTTATT	4835	0.0	208.87073	4
GGGGGAG	8015	0.0	196.24454	2
TTAGTAG	5115	0.0	195.71262	9
GTTATTA	5150	0.0	194.66795	5
GCAGAAG	8245	0.0	189.34384	8
CGGGCTA	25	5.630612E-5	176.40001	2
AGCAGAA	10340	0.0	151.12282	7
GGGGATC	50	6.0367693E-6	117.600006	3
>>END_MODULE