##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE77A l01n02 clinical flu pool a3.3420000000b6b1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	624617
Sequences flagged as poor quality	0
Sequence length	300
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85449483443454	34.0	34.0	34.0	32.0	34.0
2	33.13596972224579	34.0	34.0	34.0	34.0	34.0
3	33.18214361760887	34.0	34.0	34.0	34.0	34.0
4	33.12611568369097	34.0	34.0	34.0	34.0	34.0
5	33.15332435716607	34.0	34.0	34.0	34.0	34.0
6	36.443838384161815	38.0	38.0	38.0	34.0	38.0
7	36.6202392826324	38.0	38.0	38.0	35.0	38.0
8	36.554275660124524	38.0	38.0	38.0	34.0	38.0
9	36.48329616388923	38.0	38.0	38.0	34.0	38.0
10-14	36.56466154459453	38.0	38.0	38.0	34.4	38.0
15-19	36.580411356079004	38.0	38.0	38.0	34.6	38.0
20-24	36.46046713425987	38.0	38.0	38.0	34.0	38.0
25-29	36.3268831940213	38.0	37.8	38.0	34.0	38.0
30-34	36.41364900410972	38.0	38.0	38.0	34.0	38.0
35-39	36.34502295006381	38.0	37.6	38.0	34.0	38.0
40-44	36.197488380879804	38.0	37.0	38.0	34.0	38.0
45-49	36.23592569526606	38.0	37.0	38.0	34.0	38.0
50-54	36.119650601888836	38.0	37.0	38.0	34.0	38.0
55-59	36.11107414623682	38.0	37.0	38.0	34.0	38.0
60-64	36.049988392887165	38.0	37.0	38.0	33.8	38.0
65-69	35.72164318294251	38.0	37.0	38.0	30.8	38.0
70-74	35.88168781829505	38.0	37.0	38.0	31.8	38.0
75-79	35.781940933403995	38.0	37.0	38.0	31.0	38.0
80-84	35.58421848909011	38.0	37.0	38.0	30.8	38.0
85-89	35.63397730129023	38.0	37.0	38.0	31.0	38.0
90-94	35.54997318356689	38.0	37.0	38.0	30.0	38.0
95-99	35.336987626017226	38.0	37.0	38.0	28.2	38.0
100-104	35.351028550295624	38.0	37.0	38.0	28.2	38.0
105-109	35.08052198387171	38.0	36.4	38.0	27.2	38.0
110-114	34.91362643027647	38.0	36.0	38.0	26.8	38.0
115-119	34.954434477447776	38.0	36.0	38.0	27.0	38.0
120-124	34.58079206938012	38.0	35.6	38.0	24.4	38.0
125-129	34.46812174500534	38.0	35.2	38.0	24.0	38.0
130-134	34.441993093367614	38.0	35.0	38.0	24.2	38.0
135-139	34.021103492220035	37.8	34.2	38.0	23.2	38.0
140-144	33.8297555141791	37.2	34.0	38.0	23.0	38.0
145-149	33.565829300195155	37.0	33.8	38.0	22.6	38.0
150-154	33.28043681167819	37.0	33.4	38.0	21.2	38.0
155-159	33.01878879377282	37.0	31.6	38.0	20.4	38.0
160-164	32.72008895050887	37.0	31.2	38.0	19.0	38.0
165-169	31.830361005224	36.8	28.4	38.0	16.2	38.0
170-174	31.100995329938183	35.8	26.4	38.0	15.0	38.0
175-179	31.32866508596468	36.0	27.8	38.0	12.0	38.0
180-184	31.28718846909386	36.0	27.8	38.0	11.0	38.0
185-189	30.815554491792575	35.2	26.0	38.0	11.0	38.0
190-194	30.082989415914067	35.0	24.2	37.6	10.2	38.0
195-199	29.528691021858197	34.4	22.8	37.2	10.0	38.0
200-204	28.78063261166443	33.4	21.8	37.0	10.0	37.6
205-209	28.08030745240684	32.4	20.6	37.0	9.8	37.2
210-214	27.3331900988926	31.0	19.8	37.0	9.0	37.0
215-219	26.306958664269462	29.6	18.4	36.6	9.0	37.0
220-224	25.019467929947474	27.4	16.2	35.2	8.8	37.0
225-229	24.11281153090614	25.4	15.0	34.2	8.2	37.0
230-234	23.09672359221731	23.8	13.4	32.8	8.0	37.0
235-239	22.020814034840548	22.4	11.6	31.6	8.0	36.4
240-244	21.213636516457285	21.0	10.6	30.8	8.0	36.0
245-249	20.18193100732129	19.4	9.2	29.0	7.4	35.6
250-254	19.158391142091872	18.2	8.8	27.4	7.0	34.2
255-259	18.165792477630294	16.6	8.0	25.6	7.0	33.0
260-264	17.613442797746462	15.8	8.0	24.8	7.0	32.4
265-269	16.978319194002083	14.6	8.0	23.8	7.0	31.6
270-274	16.13514201502681	13.2	8.0	22.2	7.0	30.2
275-279	15.249552605836858	13.0	8.0	21.0	7.0	29.0
280-284	14.442785258806598	12.2	8.0	19.4	7.0	27.4
285-289	14.141485742462981	12.0	8.0	19.0	7.0	26.6
290-294	13.688330608997195	11.2	8.0	18.4	7.0	25.2
295-299	13.182806103580276	11.0	8.0	17.8	7.0	24.4
300	11.805339912298257	9.0	8.0	13.0	7.0	20.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.1041999914502938
1101	2	0.1150244557365454
1101	3	0.05672964546868542
1101	4	-0.02063710154970977
1101	5	0.14541119538910152
1101	6	0.19249924570996058
1101	7	0.1769295781778908
1101	8	0.16874898157627882
1101	9	0.16620675043966315
1101	10-14	0.08435589325453918
1101	15-19	0.12475469585152865
1101	20-24	0.021746973609829467
1101	25-29	-0.0139483628493835
1101	30-34	0.06598615975862288
1101	35-39	-0.019146637024036295
1101	40-44	0.009936372784764558
1101	45-49	0.09091005164407306
1101	50-54	0.035972051309890674
1101	55-59	0.03712894960229818
1101	60-64	0.14931753151807214
1101	65-69	0.1753345897736267
1101	70-74	0.11192046265698963
1101	75-79	0.1990196254302461
1101	80-84	-0.04312857219805721
1101	85-89	-0.06855932101592543
1101	90-94	0.055149472644494324
1101	95-99	-0.08361033060145928
1101	100-104	0.05965973491554166
1101	105-109	0.012255159157525952
1101	110-114	0.05812019285274772
1101	115-119	0.12202680864413651
1101	120-124	-0.06960689463806347
1101	125-129	-0.014645390529892666
1101	130-134	-0.09121491838481433
1101	135-139	-0.3216362167085478
1101	140-144	-0.2431447882656741
1101	145-149	0.0791904740595939
1101	150-154	-0.20183951320300508
1101	155-159	-0.1734510859616165
1101	160-164	-0.23972797804828616
1101	165-169	0.17214349363363723
1101	170-174	0.6924775397947016
1101	175-179	-0.12617395779446383
1101	180-184	-0.5538925186995947
1101	185-189	-0.4099760569948785
1101	190-194	-1.0327350068869698
1101	195-199	-1.2516365368520823
1101	200-204	-0.8671066442162463
1101	205-209	-0.7355576402767063
1101	210-214	-1.3507477275091198
1101	215-219	-1.1398732738411184
1101	220-224	-1.473711087507784
1101	225-229	-1.1751270871543333
1101	230-234	-0.9452856051823524
1101	235-239	-1.0792584333993815
1101	240-244	-1.0155626247468668
1101	245-249	-0.8295178204895279
1101	250-254	-0.49820860093275243
1101	255-259	-0.4844116245549621
1101	260-264	-0.5541444534817543
1101	265-269	-0.8305783182129787
1101	270-274	-0.5766404022514013
1101	275-279	-0.6098760227638014
1101	280-284	-0.3634084626975955
1101	285-289	-0.5621280154996384
1101	290-294	-0.49453266442985466
1101	295-299	-0.31315044749229415
1101	300	0.6989971508725645
1102	1	0.031460786733113366
1102	2	0.050542450630523206
1102	3	-0.04102332785574703
1102	4	0.08002801371232238
1102	5	0.036514319388999184
1102	6	0.2787672873053637
1102	7	0.059522180960861704
1102	8	0.18319673896633049
1102	9	0.10062056421607934
1102	10-14	0.12773377516725049
1102	15-19	0.05761222969739066
1102	20-24	-0.0708534923908104
1102	25-29	-0.07473074576635952
1102	30-34	0.09126517365609743
1102	35-39	0.09484945598494221
1102	40-44	-0.026056456192428357
1102	45-49	-0.05304663839021373
1102	50-54	-0.007295195275112576
1102	55-59	0.09739001422115479
1102	60-64	0.11548324991641579
1102	65-69	0.1887948834206341
1102	70-74	0.04341576282772053
1102	75-79	0.1446364246227816
1102	80-84	0.11549309118382212
1102	85-89	0.1621212745033489
1102	90-94	0.1365402919396388
1102	95-99	0.18706754247780566
1102	100-104	0.01243973714562685
1102	105-109	0.1135809005517956
1102	110-114	0.1807522130914876
1102	115-119	0.11198042579666634
1102	120-124	0.09153534271526098
1102	125-129	0.20739638154684314
1102	130-134	0.24422067925593893
1102	135-139	0.17692479997797506
1102	140-144	0.08191773372743683
1102	145-149	0.10529201238299635
1102	150-154	0.021552328455257452
1102	155-159	0.2221155363447167
1102	160-164	0.3231879000037807
1102	165-169	0.43194377080120105
1102	170-174	1.1138860549498553
1102	175-179	0.5142373333806205
1102	180-184	0.38192595826899733
1102	185-189	0.2313259644168255
1102	190-194	0.032511453579001426
1102	195-199	0.0922754066949345
1102	200-204	0.2960910353380797
1102	205-209	0.10867694137260031
1102	210-214	0.22237759016921643
1102	215-219	0.13620227097270288
1102	220-224	-0.21950600378551144
1102	225-229	0.28540332525188816
1102	230-234	-0.4004095124050764
1102	235-239	-0.09805963301070975
1102	240-244	0.23591683314002765
1102	245-249	0.194999489450133
1102	250-254	1.0394641762601537
1102	255-259	0.5957401949183065
1102	260-264	0.41364951573440223
1102	265-269	0.34247531819936583
1102	270-274	0.25721502170346255
1102	275-279	0.640762809367363
1102	280-284	1.0005160214507072
1102	285-289	0.5674902491765543
1102	290-294	0.38564695868597987
1102	295-299	0.2551358778943147
1102	300	0.7333266848574489
1103	1	-0.04150849016738789
1103	2	0.05295207687574788
1103	3	0.08705575050234415
1103	4	0.006868637588048898
1103	5	0.12301530418325513
1103	6	0.30573437642286194
1103	7	0.20554429085061798
1103	8	0.08385840102464215
1103	9	0.09212593844154071
1103	10-14	0.08607752564616078
1103	15-19	0.05447424247235233
1103	20-24	-0.08503693777686294
1103	25-29	-0.0304867386352754
1103	30-34	0.036922628720077455
1103	35-39	0.02189024959994157
1103	40-44	-0.06556192090658897
1103	45-49	-0.10072958061537207
1103	50-54	-0.12915062207888184
1103	55-59	-0.14403658376168238
1103	60-64	-0.2214223433792455
1103	65-69	0.041934950426053774
1103	70-74	0.05047534014337174
1103	75-79	0.05821687964953526
1103	80-84	0.44213544266378335
1103	85-89	0.23367068169296346
1103	90-94	0.24101590633230074
1103	95-99	0.3504785136476656
1103	100-104	0.13981854980265496
1103	105-109	0.22659083898299315
1103	110-114	0.30736479087344293
1103	115-119	0.1478783107694852
1103	120-124	0.2087854060208656
1103	125-129	0.34785372890009114
1103	130-134	0.2828911668805105
1103	135-139	0.4872252589161832
1103	140-144	0.43335230785045553
1103	145-149	0.4953877333625769
1103	150-154	0.391308381163455
1103	155-159	0.4747826539953408
1103	160-164	0.42885103250331014
1103	165-169	0.7595856574320017
1103	170-174	1.6163915700165923
1103	175-179	1.037530289784776
1103	180-184	0.7185387768676321
1103	185-189	0.712303417408318
1103	190-194	0.754857416652321
1103	195-199	0.6870112271537003
1103	200-204	0.6235590231396237
1103	205-209	1.0926662082463743
1103	210-214	0.9133889253371343
1103	215-219	0.8331706079584222
1103	220-224	0.8926634114751124
1103	225-229	0.7774192308283965
1103	230-234	0.9033728418625238
1103	235-239	0.7252605429751959
1103	240-244	0.774711742713361
1103	245-249	1.0320101663858061
1103	250-254	1.1036667045433397
1103	255-259	1.51413588882426
1103	260-264	0.7792214881762156
1103	265-269	0.5432267392001862
1103	270-274	0.7881750702170756
1103	275-279	0.8737196659654067
1103	280-284	1.0969385073063762
1103	285-289	0.6669851179570081
1103	290-294	0.688149700712211
1103	295-299	0.39663807771815485
1103	300	1.1093827207084832
2116	1	0.11755073185538123
2116	2	0.005343393729674517
2116	3	0.028631792154129698
2116	4	0.014306979050431323
2116	5	0.03856191418661581
2116	6	0.008632361585014792
2116	7	-0.0169621517424261
2116	8	-0.013465151805718278
2116	9	-0.0010500977872069939
2116	10-14	0.12395370694088825
2116	15-19	0.02896175723452643
2116	20-24	-0.06968046350674229
2116	25-29	0.03942399760297377
2116	30-34	0.03396685900379026
2116	35-39	-0.13841656183223705
2116	40-44	0.022433218888430417
2116	45-49	-0.01357229853403652
2116	50-54	0.005667961169898206
2116	55-59	0.0025205817245677054
2116	60-64	-0.20089889666434146
2116	65-69	0.13749066964954437
2116	70-74	0.00848982329040382
2116	75-79	0.004144534267183531
2116	80-84	0.15483635574768329
2116	85-89	0.11808713647683078
2116	90-94	0.18541822460318258
2116	95-99	0.08059005579173117
2116	100-104	0.24704909405107145
2116	105-109	0.31661397112568324
2116	110-114	0.22821849262086857
2116	115-119	0.2340609136247096
2116	120-124	0.3009721061930435
2116	125-129	0.38062860035052637
2116	130-134	0.37840071708803436
2116	135-139	0.32566939239153214
2116	140-144	0.28676927305843236
2116	145-149	0.49608517864039214
2116	150-154	0.3837870087564781
2116	155-159	0.48169290985596547
2116	160-164	0.6146267983868157
2116	165-169	0.9221611263681346
2116	170-174	1.8105900300966944
2116	175-179	1.050085037626836
2116	180-184	0.8275888200503161
2116	185-189	1.0203980550969618
2116	190-194	1.0350632571794058
2116	195-199	0.7151328480672383
2116	200-204	0.970970755126281
2116	205-209	0.9454473601486661
2116	210-214	0.959687361123553
2116	215-219	1.2969073742145945
2116	220-224	1.7561102656246277
2116	225-229	0.33741011427985157
2116	230-234	0.9972241182709283
2116	235-239	0.8854458492827995
2116	240-244	0.9671822357654776
2116	245-249	0.6690698156551775
2116	250-254	0.8982270417676972
2116	255-259	1.2404351160795137
2116	260-264	0.8882265348893696
2116	265-269	0.6175927854798609
2116	270-274	0.841241517324427
2116	275-279	0.9476487921288417
2116	280-284	0.808063517978379
2116	285-289	0.6003497436233918
2116	290-294	0.5121956234934313
2116	295-299	0.2921706391495107
2116	300	0.7361545941042476
1117	1	0.22056919550376364
1117	2	0.16863472867455442
1117	3	0.1145318654557741
1117	4	0.05991115378846246
1117	5	0.12637583349659565
1117	6	0.1844686460827205
1117	7	0.08911715591168701
1117	8	0.20583264387002487
1117	9	0.17260750894126176
1117	10-14	0.278629206183318
1117	15-19	0.14271849398339498
1117	20-24	0.261076435124167
1117	25-29	0.2608431393370765
1117	30-34	0.3454390580350548
1117	35-39	0.24651443821687025
1117	40-44	0.3508465747033611
1117	45-49	0.4109941213079722
1117	50-54	0.45012091806380283
1117	55-59	0.4562064905285794
1117	60-64	0.43376969076015115
1117	65-69	0.5840520947936483
1117	70-74	0.48921920197253854
1117	75-79	0.5365596143987972
1117	80-84	0.44765181202770066
1117	85-89	0.38416353389180813
1117	90-94	0.3948601316828828
1117	95-99	0.5176347079544996
1117	100-104	0.576079347805738
1117	105-109	0.5917745344752205
1117	110-114	0.7191505148914743
1117	115-119	0.40606157570411483
1117	120-124	0.5735893388102724
1117	125-129	0.5563414907090873
1117	130-134	0.7233010141143126
1117	135-139	0.6321936756087325
1117	140-144	0.5393292103427783
1117	145-149	0.8066736835596018
1117	150-154	0.7207418436640651
1117	155-159	0.9276036782864168
1117	160-164	1.1358211605260706
1117	165-169	1.4689714860918848
1117	170-174	1.9119291402547027
1117	175-179	1.5307631233442933
1117	180-184	1.300130531962111
1117	185-189	1.3565163417664365
1117	190-194	1.3712423678467083
1117	195-199	1.475976526391225
1117	200-204	1.3282673612417923
1117	205-209	1.1687922798715356
1117	210-214	1.0291653187904828
1117	215-219	1.4436352286589766
1117	220-224	1.6076757014420338
1117	225-229	1.3361761728884254
1117	230-234	0.7863213439035874
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2108	240-244	-1.0493177340413418
2108	245-249	0.16434522321738143
2108	250-254	-0.34395813941884157
2108	255-259	0.43200898598533044
2108	260-264	-0.1675865674874757
2108	265-269	-0.6182103256987546
2108	270-274	-0.18476311961629222
2108	275-279	-0.3612392387450587
2108	280-284	0.01701863261182801
2108	285-289	-0.307386481423773
2108	290-294	-0.1977112168175772
2108	295-299	1.066532785317447
2108	300	1.3161498729885999
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	97.0
11	886.0
12	1462.0
13	1273.0
14	1371.0
15	1879.0
16	2813.0
17	3853.0
18	5426.0
19	6504.0
20	7111.0
21	8643.0
22	11061.0
23	13688.0
24	17387.0
25	22543.0
26	27574.0
27	35047.0
28	44905.0
29	56298.0
30	67728.0
31	76718.0
32	77388.0
33	66439.0
34	44449.0
35	19062.0
36	2984.0
37	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.44057078177507	25.64851741146975	13.880826170277146	24.03008563647803
2	41.07364993267875	23.592377408876157	20.693000670811074	14.640971987634021
3	41.29378483134465	22.071765577946163	20.076622954546547	16.55782663616264
4	40.679008096161326	21.347801933024556	20.04364274427369	17.929547226540425
5	33.94175951022787	21.754771323867267	26.712529438039628	17.590939727865234
6	33.71377980426406	21.835620868468197	26.69043589911898	17.760163428148772
7	18.38518644225181	43.59871729395774	20.321412961863032	17.694683301927423
8	32.86846179338699	22.06840351767563	27.147195801587216	17.91593888735017
9	18.250864129538584	22.019573594698834	27.171050419697192	32.558511856065394
10-14	25.630970658819724	34.61887844871337	22.195489395901806	17.554661496565092
15-19	25.04584409326035	30.416143012438024	22.365033292401584	22.172979601900046
20-24	25.890489692083307	28.379470939791908	24.748189690642423	20.981849677482362
25-29	23.306346128907794	27.85140333996673	27.835841803857402	21.00640872726807
30-34	23.67447571872043	27.40200795047205	27.261153634947494	21.662362695860025
35-39	24.15886855465029	28.377773899845828	25.96820131376508	21.49515623173881
40-44	25.08676516969599	27.209986279592137	25.599303253033455	22.10394529767842
45-49	23.17833168165452	27.343668199872884	25.9432900481415	23.5347100703311
50-54	23.95938631193195	27.63949748405823	26.252791710760352	22.148324493249465
55-59	23.72010367953482	26.546027405594145	27.23864384094573	22.4952250739253
60-64	24.015548728260676	26.535236793106815	26.97326521692493	22.475949261707576
65-69	23.01109319791168	26.04853854442002	27.686118053142966	23.254250204525334
70-74	22.385525850241027	26.846339436806876	26.344015612767503	24.424119100184594
75-79	22.389624361808917	27.186099641860533	26.890686612756298	23.533589383574256
80-84	23.28111466706798	26.658576375602973	26.35605499049818	23.704253966830873
85-89	22.214284913795172	27.80039608271949	25.933780220519132	24.051538782966205
90-94	22.192191374874522	28.25488259205241	27.208673475105545	22.344252557967522
95-99	24.867366722327443	25.890553731326555	26.713393967823485	22.52868557852252
100-104	22.905940760498034	27.149597273209025	26.541928894026256	23.402533072266685
105-109	23.530931754979452	26.928950060597135	26.44225181191034	23.097866372513074
110-114	23.328439667828444	26.897410733297367	26.61131541408575	23.162834184788437
115-119	22.018580986428482	26.365276641525924	27.43021723712291	24.18592513492268
120-124	23.777963135809625	25.387141240151966	27.73004897401127	23.104846650027135
125-129	23.513481061194298	25.821807603699547	26.533123498079625	24.13158783702653
130-134	23.524463791411378	27.359101657495717	26.589446012516472	22.52698853857644
135-139	24.241062923359436	26.46930839218273	26.28554778368184	23.004080900775996
140-144	22.9642805110972	27.08145951839287	27.055011310931338	22.89924865957859
145-149	23.046731036779338	27.195257253645032	25.882484786677274	23.875526922898352
150-154	22.86985464692764	26.36018552168769	26.61153955143712	24.158420279947553
155-159	22.414727745162235	25.296301573604307	27.76088386963531	24.528086811598147
160-164	22.6243281883138	27.700206686657587	26.84528278929328	22.830182335735337
165-169	21.745997947542254	27.42807192247409	27.268998442245408	23.55693168773825
170-174	24.043501857938544	27.280045211705733	26.315005835576038	22.36144709477968
175-179	23.180573055168207	26.975314472708877	27.00951142860345	22.834601043519466
180-184	23.721064268183543	25.649574058983344	27.383084354092187	23.246277318740923
185-189	24.581535431003456	26.088059110802014	26.394454968230118	22.93595048996441
190-194	23.693463354343542	25.79894559385992	27.311296362410886	23.196294689385656
195-199	24.845913575839273	26.593000190516747	26.293040375141885	22.268045858502088
200-204	23.927302651064572	26.07943747928731	26.38717806271683	23.606081806931286
205-209	24.26322050152333	26.038964677554404	25.79811308369769	23.89970173722457
210-214	23.89720420673789	27.568317865187787	25.95427277835858	22.580205149715745
215-219	24.756834988480943	25.977903259117184	26.416187839908297	22.849073912493576
220-224	24.380316257802782	26.65050743095369	26.249173493516825	22.7200028177267
225-229	25.219006207003652	26.723416109391835	25.785433313534533	22.27214437006998
230-234	24.881871610923174	26.060417824042574	25.469783883563846	23.587926681470407
235-239	25.49443899221443	26.054077938960994	25.577593949572297	22.873889119252276
240-244	25.4484908351838	27.61724384702946	24.836467787460155	22.097797530326584
245-249	25.03534165736763	27.013417822441593	24.939378851360114	23.01186166883066
250-254	24.776302918428414	27.44107188885349	24.786100922645396	22.9965242700727
255-259	24.872906116868418	26.96442780135667	26.04210260047357	22.120563481301343
260-264	24.944117755360484	27.491951067614234	25.181831426298036	22.382099750727246
265-269	25.167550675053675	27.88178996088803	24.212405362005835	22.738254002052457
270-274	25.07543022364105	28.140284366259642	23.833164963489626	22.951120446609682
275-279	24.958014271145355	27.357564715657755	23.947923287390513	23.736497725806373
280-284	23.6092837690937	28.384978314711258	23.957080899175015	24.04865701702003
285-289	25.335013296147878	28.032186123656576	24.214870872870893	22.41792970732465
290-294	24.12553612853957	29.10999860714646	23.365070114966453	23.39939514934752
295-299	23.83271668878689	29.539894047072046	22.727847625024616	23.89954163911645
300	17.352553644873577	38.92449292926706	19.262363976644888	24.460589449214478
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	0.25
4	0.0
5	0.0
6	0.25
7	0.0
8	0.75
9	1.0
10	0.5
11	0.25
12	0.75
13	1.5
14	1.75
15	2.75
16	4.5
17	6.25
18	7.25
19	11.25
20	16.5
21	22.5
22	30.25
23	41.0
24	69.5
25	104.0
26	174.0
27	228.25
28	337.75
29	466.25
30	575.25
31	828.25
32	1191.75
33	1939.75
34	2892.25
35	3175.25
36	3615.5
37	4072.5
38	4855.75
39	6228.75
40	7705.75
41	8451.25
42	10045.75
43	11015.75
44	11191.0
45	10851.0
46	10121.75
47	10002.5
48	10461.5
49	10782.0
50	9720.0
51	8215.75
52	7679.0
53	7217.5
54	6369.75
55	6151.25
56	6832.5
57	6945.75
58	5077.75
59	3246.5
60	2294.75
61	1660.5
62	1209.25
63	976.75
64	824.25
65	667.0
66	495.0
67	329.25
68	214.5
69	144.75
70	103.25
71	69.0
72	53.75
73	32.5
74	31.5
75	22.5
76	18.5
77	11.75
78	11.25
79	8.75
80	7.5
81	6.25
82	4.25
83	3.25
84	3.0
85	2.75
86	3.25
87	1.25
88	1.25
89	1.25
90	1.75
91	0.5
92	0.75
93	1.5
94	0.75
95	0.25
96	0.0
97	0.0
98	0.25
99	0.5
100	0.3333333333333333
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	3.2019621624131265E-5
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	624617.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.67196553519244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.09155883021825	32.043044103869484
2	8.886790388867082	7.584336263844311
3	4.812770501081145	6.161111308527765
4	2.996623834998856	5.114873160360295
5	1.8791450968994396	4.009340740525938
6	1.5238903357806204	3.901643753270608
7	1.074718983706246	3.2102260002910983
8	0.7473261560545797	2.55119007797671
9	0.5668778694929	2.1770813618697913
>10	2.302230673979874	15.848766752947366
>50	0.050451154639727075	1.4953009324877051
>100	0.04575965733405669	4.290443192073812
>500	0.011305529265628812	3.3960804408287353
>1k	0.010174166696040297	7.250333211896997
>5k	3.768209857216102E-4	0.9662286992293961
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGAGCGAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCA	6009	0.9620295316970239	No Hit
GGGGGGAGCGAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAA	3178	0.5087917876074458	No Hit
GGGGGGAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCA	2576	0.4124127265188108	No Hit
CGGGTTATTAGTAGAAACAAGGAAGGACTTGCCCCTCAGGGGAGGCATCA	2547	0.40776988138331166	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	2435	0.38983889327379817	No Hit
GGGGGGAGCGAAAGCAGGATCCCTGATGCCTATGACAGTGGCTGCCACAT	2325	0.372228101380526	No Hit
GGGGGGAGCGAAAGCAGGTGCAGGATAAATGAAGGAACTGAACCAGCTAG	2113	0.33828730245894684	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	2061	0.3299622008366727	No Hit
GGGGGGAGCGAAAGCAGGAAAAAATGTACAATATTGAAAAGTGAAAATAT	1777	0.2844943381304063	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT	1722	0.2756889421837702	Illumina Single End PCR Primer 1 (97% over 34bp)
CGGGTTATTAGTAGAAACAAGGAAGGAATAAACATACCGGGACCGATACT	1710	0.2737677648863224	No Hit
GGGGGGAGCGAAAGCAGGACAGGCAAGGCTGGTAAGAAGGAACCAGTTAA	1595	0.25535648245244685	No Hit
GGGGGGAGCGAAAGCAGGAGAGAAGGGACAACATTGATACAGTTATCAAT	1592	0.2548761881280849	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT	1591	0.25471609001996426	No Hit
GGGGGGAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAA	1472	0.23566441515360612	No Hit
GGGGGGAGCGAAAGCAGGAGGATGAGTGACCACAATCCCTCCGGGACCCT	1450	0.23214225677495168	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1439	0.23038117758562446	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	1413	0.22621862677448742	No Hit
CGGGTTATTAGTAGAAACAAGGACACCTACCTTAAGGATTTGGTGTGAGG	1386	0.2218959778552297	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	1369	0.21917431001717852	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	1345	0.21533195542228276	No Hit
CGGGTTATTAGTAGAAACAAGGAGGATAGGAGAACATGCTAGGAGGGTAG	1245	0.19932214461021716	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	1242	0.19884185028585516	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	1163	0.1861940997443233	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	1153	0.18459311866311676	No Hit
GGGGGGAGCAAAAGCAGGATCCCTGATGCCTATGACAGTGGCTGCCACAT	1098	0.17578772271648066	No Hit
GGGGGGAGCGAAAGCAGGACAGGCAAGGCTGGCAAGAGGGAACAAGTTAA	1081	0.17306605487842952	No Hit
GGGGGGAGCGAAAGCAGGTGCCTCTCCCAGGGTGGAGGAGAGGAGCGGGG	1012	0.16201928541810423	No Hit
GGGGGGAGCAAAAGCAGGAAAAAATGTACAATATTGAAAAGTGAAAATAT	992	0.1588173232556911	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	960	0.15369418379583008	No Hit
CGGGTTATTAGTAGAAACAAGGTAAAATGTGTGAATATAAAAGCCACGCT	959	0.15353408568770943	No Hit
GGGGGGAGCAAAAGCAGGTGCAGGATAAATGAAGGAACTGAACCAGCTAG	950	0.1520932027146235	No Hit
CGGGTTATTAGTAGAAACAAGGACAAGTTTAGAAGCAAGGACAGTGTCAG	916	0.1466498670385212	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	896	0.14344790487610806	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	896	0.14344790487610806	No Hit
CGGGTTATTAGTAGAAACAAGGGTCAGAAATGGGGTAGGAACTGTTAGCA	825	0.13208093919954147	No Hit
GGGGGGAGCGAAAGCAGGTGGATCATAAAGTCAGCAGTTCAAGACCAGCC	805	0.12887897703712836	No Hit
GGGGGGAGCGAAAGCAGGGAGTCAAGGATGGCCAAGGTTTGTGGCCTGAG	772	0.1235957394691467	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	724	0.11591103027935518	No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	709	0.11350955865754533	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	702	0.11238887190070075	No Hit
GGGGGGAGCAAAAGCAGGAGAGAAGGGACAACATTGATACAGTTATCAAT	690	0.11046769460325287	No Hit
GGGGGGAGCGAAAGCAGGCCTGAGGACTGATCACCTGGGTGGATTTTCCA	673	0.10774602676520172	No Hit
GGGGGGAGCAAAAGCAGGACAGGCAAGGCTGGTAAGAAGGAACCAGTTAA	669	0.10710563433271908	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	657	0.10518445703527123	No Hit
GGGGGGAGCAAAAGCAGGCCTGAGGACTGATCACCTGGGTGGATTTTCCA	642	0.10278298541346136	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	4.80294324361969E-4	0.0	0.0
2	6.403924324826254E-4	0.0	0.0
3	8.004905406032817E-4	0.0	0.0
4	8.004905406032817E-4	0.0	0.0
5	9.60588648723938E-4	0.0	0.0
6	0.0012807848649652508	0.0	0.0
7	0.002241373513689189	0.0	0.0
8	0.002401471621809845	0.0	0.0
9	0.002881765946171814	0.0	0.0
10-14	0.003842354594895752	0.0	0.0
15-19	0.007236434487053666	0.0	0.0
20-24	0.01088667135220463	0.0	0.0
25-29	0.014184692379490152	0.0	0.0
30-34	0.017514733028399804	0.0	0.0
35-39	0.021357087623295558	0.0	0.0
40-44	0.025807815029049802	0.0	0.0
45-49	0.033012229894479335	0.0	0.0
50-54	0.04226590054385328	0.0	0.0
55-59	0.05427325865290249	0.0	0.0
60-64	0.06887420611350635	0.0	0.0
65-69	0.08901454811508493	0.0	0.0
70-74	0.11491842200900712	0.0	0.0
75-79	0.15046020201179283	0.0	0.0
80-84	0.19602412358293164	0.0	0.0
85-89	0.27911504169755225	0.0	0.0
90-94	0.4247402808441013	0.0	0.0
95-99	0.5757768360451284	0.0	0.0
100-104	0.6938972202165486	0.0	0.0
105-109	0.8342392217951161	0.0	0.0
110-114	0.998403821862037	0.0	0.0
115-119	1.1764329180922068	0.0	0.0
120-124	1.395959443947251	0.0	0.0
125-129	1.6473134736966812	0.0	0.0
130-134	1.9333127340434217	0.0	0.0
135-139	2.2704473301238997	0.0	0.0
140-144	2.643860157504519	0.0	0.0
145-149	3.077886128619618	0.0	0.0
150-154	3.569803575631147	0.0	0.0
155-159	4.1261124817288035	0.0	0.0
160-164	4.690522352097365	0.0	0.0
165-169	5.303089733388621	0.0	0.0
170-174	6.025324318742525	0.0	0.0
175-179	6.848772928050309	0.0	0.0
180-184	7.758034123310765	0.0	0.0
185-189	8.735561151873869	3.201962162413127E-5	0.0
190-194	9.78112987638825	1.6009810812065635E-4	0.0
195-199	10.899223043881289	1.6009810812065635E-4	0.0
200-204	12.06931607689192	1.6009810812065635E-4	0.0
205-209	13.286862189149511	1.6009810812065635E-4	0.0
210-214	14.52755848784135	1.6009810812065635E-4	0.0
215-219	15.750003602207432	1.6009810812065635E-4	0.0
220-224	16.98573685954753	1.6009810812065635E-4	0.0
225-229	18.23459816175352	1.6009810812065635E-4	0.0
230-234	19.45761962930884	1.6009810812065635E-4	0.0
235-239	20.655281556537847	1.6009810812065635E-4	0.0
240-244	21.764377210354503	1.6009810812065635E-4	0.0
245-249	22.8085050518958	1.6009810812065635E-4	0.0
250-254	23.752475516996817	1.6009810812065635E-4	0.0
255-259	24.669421421447062	1.6009810812065635E-4	0.0
260-264	25.53417534264998	1.6009810812065635E-4	0.0
265-269	26.41663611461103	1.6009810812065635E-4	1.6009810812065635E-4
270-274	27.09461958288039	1.6009810812065635E-4	1.6009810812065635E-4
275-279	27.683844660007658	1.6009810812065635E-4	1.6009810812065635E-4
280-284	28.215082202373612	1.6009810812065635E-4	1.6009810812065635E-4
285-288	28.667367362719872	1.6009810812065635E-4	1.6009810812065635E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	4100	0.0	286.47073	1
GGGTTAT	4340	0.0	270.62903	2
GGTTATT	4500	0.0	260.68	3
GGGGGGA	10870	0.0	256.4048	1
TATTAGT	4690	0.0	250.11938	6
ATTAGTA	4755	0.0	246.70032	7
GGGAGCG	7360	0.0	245.46603	4
GGGGAGC	11360	0.0	244.69806	3
GGAGCGA	7385	0.0	244.63507	5
GAGCGAA	7590	0.0	237.834	6
GCGAAAG	7685	0.0	235.65906	8
GTTATTA	4975	0.0	234.31357	4
AGCGAAA	7735	0.0	234.13574	7
CGAAAGC	7770	0.0	233.64865	9
GGGGGAG	11960	0.0	233.28262	2
TTATTAG	5115	0.0	228.47508	5
TTAGTAG	6015	0.0	195.02246	8
TAGTAGA	6110	0.0	192.47136	9
GAGCAAA	5160	0.0	187.73836	6
GCAAAAG	5300	0.0	182.77924	8
>>END_MODULE