FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n02 clinical flu pool a3.3420000000b6b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n02 clinical flu pool a3.3420000000b6b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624617
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCGAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCA60090.9620295316970239No Hit
GGGGGGAGCGAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAA31780.5087917876074458No Hit
GGGGGGAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCA25760.4124127265188108No Hit
CGGGTTATTAGTAGAAACAAGGAAGGACTTGCCCCTCAGGGGAGGCATCA25470.40776988138331166No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA24350.38983889327379817No Hit
GGGGGGAGCGAAAGCAGGATCCCTGATGCCTATGACAGTGGCTGCCACAT23250.372228101380526No Hit
GGGGGGAGCGAAAGCAGGTGCAGGATAAATGAAGGAACTGAACCAGCTAG21130.33828730245894684No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC20610.3299622008366727No Hit
GGGGGGAGCGAAAGCAGGAAAAAATGTACAATATTGAAAAGTGAAAATAT17770.2844943381304063No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT17220.2756889421837702Illumina Single End PCR Primer 1 (97% over 34bp)
CGGGTTATTAGTAGAAACAAGGAAGGAATAAACATACCGGGACCGATACT17100.2737677648863224No Hit
GGGGGGAGCGAAAGCAGGACAGGCAAGGCTGGTAAGAAGGAACCAGTTAA15950.25535648245244685No Hit
GGGGGGAGCGAAAGCAGGAGAGAAGGGACAACATTGATACAGTTATCAAT15920.2548761881280849No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT15910.25471609001996426No Hit
GGGGGGAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAA14720.23566441515360612No Hit
GGGGGGAGCGAAAGCAGGAGGATGAGTGACCACAATCCCTCCGGGACCCT14500.23214225677495168No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT14390.23038117758562446No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG14130.22621862677448742No Hit
CGGGTTATTAGTAGAAACAAGGACACCTACCTTAAGGATTTGGTGTGAGG13860.2218959778552297No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA13690.21917431001717852No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT13450.21533195542228276No Hit
CGGGTTATTAGTAGAAACAAGGAGGATAGGAGAACATGCTAGGAGGGTAG12450.19932214461021716No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT12420.19884185028585516No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT11630.1861940997443233No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA11530.18459311866311676No Hit
GGGGGGAGCAAAAGCAGGATCCCTGATGCCTATGACAGTGGCTGCCACAT10980.17578772271648066No Hit
GGGGGGAGCGAAAGCAGGACAGGCAAGGCTGGCAAGAGGGAACAAGTTAA10810.17306605487842952No Hit
GGGGGGAGCGAAAGCAGGTGCCTCTCCCAGGGTGGAGGAGAGGAGCGGGG10120.16201928541810423No Hit
GGGGGGAGCAAAAGCAGGAAAAAATGTACAATATTGAAAAGTGAAAATAT9920.1588173232556911No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG9600.15369418379583008No Hit
CGGGTTATTAGTAGAAACAAGGTAAAATGTGTGAATATAAAAGCCACGCT9590.15353408568770943No Hit
GGGGGGAGCAAAAGCAGGTGCAGGATAAATGAAGGAACTGAACCAGCTAG9500.1520932027146235No Hit
CGGGTTATTAGTAGAAACAAGGACAAGTTTAGAAGCAAGGACAGTGTCAG9160.1466498670385212No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG8960.14344790487610806No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT8960.14344790487610806No Hit
CGGGTTATTAGTAGAAACAAGGGTCAGAAATGGGGTAGGAACTGTTAGCA8250.13208093919954147No Hit
GGGGGGAGCGAAAGCAGGTGGATCATAAAGTCAGCAGTTCAAGACCAGCC8050.12887897703712836No Hit
GGGGGGAGCGAAAGCAGGGAGTCAAGGATGGCCAAGGTTTGTGGCCTGAG7720.1235957394691467No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT7240.11591103027935518No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA7090.11350955865754533No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG7020.11238887190070075No Hit
GGGGGGAGCAAAAGCAGGAGAGAAGGGACAACATTGATACAGTTATCAAT6900.11046769460325287No Hit
GGGGGGAGCGAAAGCAGGCCTGAGGACTGATCACCTGGGTGGATTTTCCA6730.10774602676520172No Hit
GGGGGGAGCAAAAGCAGGACAGGCAAGGCTGGTAAGAAGGAACCAGTTAA6690.10710563433271908No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT6570.10518445703527123No Hit
GGGGGGAGCAAAAGCAGGCCTGAGGACTGATCACCTGGGTGGATTTTCCA6420.10278298541346136No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA41000.0286.470731
GGGTTAT43400.0270.629032
GGTTATT45000.0260.683
GGGGGGA108700.0256.40481
TATTAGT46900.0250.119386
ATTAGTA47550.0246.700327
GGGAGCG73600.0245.466034
GGGGAGC113600.0244.698063
GGAGCGA73850.0244.635075
GAGCGAA75900.0237.8346
GCGAAAG76850.0235.659068
GTTATTA49750.0234.313574
AGCGAAA77350.0234.135747
CGAAAGC77700.0233.648659
GGGGGAG119600.0233.282622
TTATTAG51150.0228.475085
TTAGTAG60150.0195.022468
TAGTAGA61100.0192.471369
GAGCAAA51600.0187.738366
GCAAAAG53000.0182.779248
Produced by FastQC (version 0.11.2)