FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n02 clinical flu pool a1.3420000000b57f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n02 clinical flu pool a1.3420000000b57f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434829
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT178044.094483118651239Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG54591.2554360449740014No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG54261.2478468547405992No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG47951.1027323384594863No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC42320.9732561535684142No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA39830.9159922636254711No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA38780.8918448401555553No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATACTCCTC36460.8384905330601224No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA35230.8102035512810783No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA33870.7789268885009969No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT33640.7736374528837773No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC28300.6508305563796343No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAGTA26320.6052954149792217No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTCAAATT26140.6011558566700933No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG25920.596096396514492No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG19390.44592242007777766No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA18630.4284442849947911No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG14580.33530422303940166No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC12890.2964383700259182No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAGTA12690.2918388607935533No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT12370.2844796460217695No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA10980.2525130568568334No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA9100.20927767007260326No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA8750.20122852891596466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA29850.0291.045231
GGGTTAT30000.0289.592
GGTTATT30900.0280.20393
ATTAGTA30950.0276.42657
GGGGGGA46050.0273.570041
GGGGGAG48100.0263.438662
GTTATTA33050.0260.196664
GGAGCGA36700.0247.937355
TTAGTAG35150.0242.978678
GCGAAAG37550.0241.541938
CGAAAGC37800.0240.333349
GAGCGAA37800.0240.333346
GGGGAGC53150.0237.855133
TATTAGT36300.0235.685946
TTATTAG37900.0226.899735
GGGAGCG41100.0222.109484
TAGTAGA38600.0221.642479
AGCGAAA41400.0219.07977
GAGCAAA23500.0149.502146
GCAAAAG25650.0137.543858
Produced by FastQC (version 0.11.2)