FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool f4.3410000000b78d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool f4.3410000000b78d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588453
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT563219.571027762625052TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGACCTCGTAT17240.2929715712214909TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGAACTCGTAT15890.2700300618741004TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG254200.080.959383
AGAGCAC249600.080.920188
CGGAAGA254700.080.858174
GAGCACA250300.080.34159
ATCGGAA257500.079.9789352
GAAGAGC259750.078.0976946
GATCGGA269300.076.583641
GGAAGAG270150.076.288265
AAGAGCA266650.076.021667
AGAGTAC4950.041.5755048
AGAGTGG1701.1158929E-534.5880248
GCTTGAA148850.026.98028660-64
CTTGAAA150500.026.80169960-64
TGCTTGA150650.026.26761460-64
CGTCTTC161700.025.18166550-54
GTCTTCT162400.024.946450-54
GAGTACA8301.8189894E-1224.795039
TGAAAAA166200.024.58829960-64
TTGAAAA166400.024.55874460-64
CCGTCTT165400.024.5472550-54
Produced by FastQC (version 0.11.2)