##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE77A l01n01 clinical flu pool f3.3410000000b713.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	687459
Sequences flagged as poor quality	0
Sequence length	300
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.794626297713755	34.0	34.0	34.0	34.0	34.0
2	33.847941477237185	34.0	34.0	34.0	34.0	34.0
3	33.846606124874356	34.0	34.0	34.0	34.0	34.0
4	33.83813871081767	34.0	34.0	34.0	34.0	34.0
5	33.867637197272856	34.0	34.0	34.0	34.0	34.0
6	37.607940255346136	38.0	38.0	38.0	37.0	38.0
7	37.62815091518185	38.0	38.0	38.0	37.0	38.0
8	37.584746144861	38.0	38.0	38.0	37.0	38.0
9	37.55107431861391	38.0	38.0	38.0	37.0	38.0
10-14	37.398312626643914	38.0	38.0	38.0	37.0	38.0
15-19	37.431654251380806	38.0	38.0	38.0	37.0	38.0
20-24	37.49332949310432	38.0	38.0	38.0	37.0	38.0
25-29	37.43029969787289	38.0	38.0	38.0	37.0	38.0
30-34	37.33994056372817	38.0	38.0	38.0	37.0	38.0
35-39	37.4071652273081	38.0	38.0	38.0	37.0	38.0
40-44	37.28288930685321	38.0	38.0	38.0	37.0	38.0
45-49	37.37224096273378	38.0	38.0	38.0	37.0	38.0
50-54	37.38215980880314	38.0	38.0	38.0	37.0	38.0
55-59	37.29030764016472	38.0	38.0	38.0	37.0	38.0
60-64	37.298238294938315	38.0	38.0	38.0	37.0	38.0
65-69	37.301224363925705	38.0	38.0	38.0	37.0	38.0
70-74	37.26302426762905	38.0	38.0	38.0	37.0	38.0
75-79	36.74018756027632	38.0	38.0	38.0	36.0	38.0
80-84	36.600573416014626	38.0	38.0	38.0	35.8	38.0
85-89	36.62986272635895	38.0	38.0	38.0	36.0	38.0
90-94	36.40652606191787	38.0	38.0	38.0	34.6	38.0
95-99	36.238023794873584	38.0	38.0	38.0	33.8	38.0
100-104	36.54306016795183	38.0	38.0	38.0	35.2	38.0
105-109	36.513278319143396	38.0	38.0	38.0	35.0	38.0
110-114	36.38774326905313	38.0	38.0	38.0	34.8	38.0
115-119	36.319853547629755	38.0	38.0	38.0	34.0	38.0
120-124	36.245829060351234	38.0	38.0	38.0	34.0	38.0
125-129	36.27543257125152	38.0	38.0	38.0	34.0	38.0
130-134	36.30674905703468	38.0	38.0	38.0	34.0	38.0
135-139	36.27990905639464	38.0	38.0	38.0	34.0	38.0
140-144	36.22572778885723	38.0	38.0	38.0	34.0	38.0
145-149	36.15649776932152	38.0	37.6	38.0	34.0	38.0
150-154	36.14984980922499	38.0	38.0	38.0	34.0	38.0
155-159	36.08657010818099	38.0	37.4	38.0	33.8	38.0
160-164	36.03017983617932	38.0	37.0	38.0	33.4	38.0
165-169	35.91453133932351	38.0	37.0	38.0	32.2	38.0
170-174	35.8579871672347	38.0	37.0	38.0	32.0	38.0
175-179	35.756170186149276	38.0	37.0	38.0	31.4	38.0
180-184	35.642034215858686	38.0	37.0	38.0	31.0	38.0
185-189	35.419632007145154	38.0	37.0	38.0	29.0	38.0
190-194	35.27973857350038	38.0	37.0	38.0	28.2	38.0
195-199	35.04314759134727	38.0	36.4	38.0	26.6	38.0
200-204	34.976010205699545	38.0	36.0	38.0	25.8	38.0
205-209	34.74213065797379	38.0	35.8	38.0	24.4	38.0
210-214	34.51277792566538	38.0	35.4	38.0	23.2	38.0
215-219	33.658668516958826	38.0	34.2	38.0	20.4	38.0
220-224	33.6892754331531	38.0	34.0	38.0	20.8	38.0
225-229	33.44357583506798	38.0	34.0	38.0	19.8	38.0
230-234	32.91190281893175	37.4	33.0	38.0	17.8	38.0
235-239	32.71565286075242	37.0	32.4	38.0	17.0	38.0
240-244	32.02990258328133	37.0	29.4	38.0	15.4	38.0
245-249	31.118122244381123	36.8	26.4	38.0	12.0	38.0
250-254	30.31235724603213	36.6	24.8	37.0	10.2	38.0
255-259	29.91368183411665	36.0	23.8	37.0	10.0	37.8
260-264	29.322976061117828	34.6	22.6	37.0	9.8	37.2
265-269	28.236087679410694	32.8	20.8	37.0	9.2	37.0
270-274	27.72351718429754	32.0	19.8	37.0	9.0	37.0
275-279	26.961687024244355	31.0	18.6	37.0	8.8	37.0
280-284	25.66886170666178	28.8	17.0	36.4	8.0	37.0
285-289	24.66162200218486	27.0	14.8	35.6	8.0	37.0
290-294	24.002001282985606	25.4	13.6	34.4	8.0	37.0
295-299	22.758046370765385	23.4	12.4	32.8	8.0	37.0
300	15.531109491620592	13.0	8.0	21.0	7.0	29.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.15323020169370238
1101	2	0.0125808112767416
1101	3	0.05368407022643851
1101	4	0.04928694640913989
1101	5	0.022748168196812912
1101	6	0.08575744996564083
1101	7	0.025067988708727285
1101	8	0.04881559420736892
1101	9	0.0991852972655991
1101	10-14	0.28139614117471723
1101	15-19	0.20965708870928523
1101	20-24	0.12798866565017875
1101	25-29	0.07857872922689069
1101	30-34	0.1922847591662631
1101	35-39	0.08764130684314608
1101	40-44	0.1455424039836828
1101	45-49	0.10814646635726888
1101	50-54	0.10083225996564238
1101	55-59	0.1113424045316691
1101	60-64	0.13148042098129764
1101	65-69	0.11455352581101863
1101	70-74	0.1241480218377049
1101	75-79	0.19073323305710943
1101	80-84	0.25777545648592337
1101	85-89	0.15823151317714235
1101	90-94	0.3590893882153239
1101	95-99	0.46968399426938134
1101	100-104	0.12685113175002272
1101	105-109	0.051448377469476725
1101	110-114	0.18303992365456168
1101	115-119	0.22621832997884184
1101	120-124	0.2409702810283818
1101	125-129	0.1802581742830256
1101	130-134	0.13054073851752435
1101	135-139	0.15527694193718844
1101	140-144	0.185366708406157
1101	145-149	0.11871917180264546
1101	150-154	0.18682837926680662
1101	155-159	0.09360890575981529
1101	160-164	0.10740092330726014
1101	165-169	0.045067961266077816
1101	170-174	0.1459469760417207
1101	175-179	0.03946872386349298
1101	180-184	0.11755312893116354
1101	185-189	0.1504675826024524
1101	190-194	0.13051889617074863
1101	195-199	0.27921823107410404
1101	200-204	0.02521620661014623
1101	205-209	0.24894404735636755
1101	210-214	0.4080099086094364
1101	215-219	0.9444484718260924
1101	220-224	0.6498640770837127
1101	225-229	0.3279378378806186
1101	230-234	0.4798108495098319
1101	235-239	-0.23673490277155196
1101	240-244	0.18139094165517378
1101	245-249	0.4754025187780542
1101	250-254	0.43036600919748835
1101	255-259	0.16414526626820702
1101	260-264	-0.5930615899539511
1101	265-269	-0.5301592834840712
1101	270-274	-0.9367322374070213
1101	275-279	-0.9003776564680805
1101	280-284	-0.3060978766580966
1101	285-289	0.23270384596387572
1101	290-294	-0.273548183836855
1101	295-299	0.2856935935459006
1101	300	-0.8239635316984764
1102	1	-0.005317793568181628
1102	2	0.03443206278299016
1102	3	0.009676851076157789
1102	4	0.04818864214992402
1102	5	0.014487075595056353
1102	6	0.11391973135215494
1102	7	0.04703799210249571
1102	8	0.06624168019941123
1102	9	0.03411740072321123
1102	10-14	0.23888646169027794
1102	15-19	0.13036771929386504
1102	20-24	0.04984335312475707
1102	25-29	0.10569623512378712
1102	30-34	0.17746452360351128
1102	35-39	0.0033576670555959254
1102	40-44	0.06319484192376024
1102	45-49	0.08686329494644696
1102	50-54	0.0926687609201764
1102	55-59	0.09298866737361067
1102	60-64	0.07155333584584866
1102	65-69	0.07567391671570789
1102	70-74	0.1346655208626757
1102	75-79	0.12836799685840106
1102	80-84	0.13917595717281728
1102	85-89	0.06006390905373138
1102	90-94	0.2669528729744002
1102	95-99	0.41370121992387965
1102	100-104	-0.023891911469029026
1102	105-109	-0.05642234090710474
1102	110-114	0.0457844425315983
1102	115-119	0.1680510634242509
1102	120-124	0.10994632027316698
1102	125-129	0.005652331087354412
1102	130-134	-0.028922490894856878
1102	135-139	0.01641445240332473
1102	140-144	-0.03604299103100317
1102	145-149	0.05441809899689076
1102	150-154	0.08787767576736627
1102	155-159	0.015472153015103629
1102	160-164	0.08888165686840921
1102	165-169	-0.07664904635514347
1102	170-174	0.07735021947524956
1102	175-179	0.07497794943842706
1102	180-184	0.10221134954565514
1102	185-189	0.2515939774096836
1102	190-194	0.30686870426890067
1102	195-199	0.3110829190248836
1102	200-204	0.18532853263411653
1102	205-209	0.23941170675666967
1102	210-214	0.3594269441403597
1102	215-219	0.9696016286069238
1102	220-224	0.7168632892222035
1102	225-229	0.5629319093282703
1102	230-234	0.6984092035001623
1102	235-239	0.5794935372807544
1102	240-244	0.672155058813253
1102	245-249	0.8354084503444064
1102	250-254	0.9179859742229404
1102	255-259	0.5945111486700796
1102	260-264	0.645717532138061
1102	265-269	0.5478426225741515
1102	270-274	-0.3938686531857094
1102	275-279	-0.14963147868143523
1102	280-284	0.18308937216207255
1102	285-289	0.32280017966511565
1102	290-294	0.5567137153132578
1102	295-299	0.8129157457761771
1102	300	0.35050981573639284
1103	1	-0.07523907527949802
1103	2	-0.05201768498724846
1103	3	-0.029585206672628317
1103	4	0.04085063924213728
1103	5	-0.01899878087248652
1103	6	0.030362442598899975
1103	7	-0.027520000904260655
1103	8	-0.0031484807509229995
1103	9	0.038996077051244527
1103	10-14	0.24190348009795315
1103	15-19	0.20311202657804017
1103	20-24	0.05725319409812357
1103	25-29	0.07355642562391296
1103	30-34	0.06704911623207721
1103	35-39	0.009063533159661574
1103	40-44	-0.003609389127895213
1103	45-49	0.09044864563193045
1103	50-54	0.119496340846041
1103	55-59	0.09614389139865409
1103	60-64	0.08769363588849899
1103	65-69	0.0972479398307371
1103	70-74	0.13160287476313925
1103	75-79	0.054304333904568125
1103	80-84	0.01098113955283253
1103	85-89	-0.05078282728883465
1103	90-94	0.2486737222746669
1103	95-99	0.32621676354717266
1103	100-104	0.014459636370766304
1103	105-109	-0.035983780036964674
1103	110-114	0.07692705551949075
1103	115-119	0.17623044337457827
1103	120-124	0.2266582890058615
1103	125-129	0.16884328778703406
1103	130-134	0.17284448505623118
1103	135-139	0.150929937714551
1103	140-144	0.16938922915296217
1103	145-149	0.22662470286887526
1103	150-154	0.08815534942836933
1103	155-159	0.09420799543064362
1103	160-164	0.027034853497013955
1103	165-169	0.12578881897823635
1103	170-174	0.09663582447950603
1103	175-179	0.10617172074751124
1103	180-184	0.09568166013558255
1103	185-189	0.2688404634311681
1103	190-194	0.32963668204538976
1103	195-199	0.4355207561039691
1103	200-204	0.3777242179016298
1103	205-209	0.2971509357809339
1103	210-214	0.345626040183042
1103	215-219	1.1336155068795648
1103	220-224	0.7346090347705783
1103	225-229	0.7081720062705799
1103	230-234	0.8857598727514002
1103	235-239	0.6363499095426093
1103	240-244	0.9350900736182055
1103	245-249	1.3940593615306192
1103	250-254	1.253108446101649
1103	255-259	1.1835715638820758
1103	260-264	1.3248030666267958
1103	265-269	1.3949061138981236
1103	270-274	1.0043494020947321
1103	275-279	0.34506630098129776
1103	280-284	0.3076489432016558
1103	285-289	0.8542145449337255
1103	290-294	1.0394726697477878
1103	295-299	1.3877742262898778
1103	300	-0.1130642272167588
2116	1	-0.06675457160123699
2116	2	-0.07939291137557092
2116	3	-0.03601250672100775
2116	4	-0.015171065260325634
2116	5	-0.01811301127809628
2116	6	-0.027995235659602713
2116	7	-0.03276357705136945
2116	8	-0.0820751977800569
2116	9	-0.016860163892474134
2116	10-14	0.23257553192402014
2116	15-19	0.11138442515816394
2116	20-24	0.05087193608348173
2116	25-29	0.07576680294677374
2116	30-34	0.07470252523101806
2116	35-39	0.06249351479679888
2116	40-44	0.0023357884786534555
2116	45-49	0.0832967231072459
2116	50-54	0.056343156961467855
2116	55-59	0.04011451322389803
2116	60-64	0.029031948316067258
2116	65-69	0.05531599691086342
2116	70-74	0.03246862086889735
2116	75-79	0.0050477508829231965
2116	80-84	-0.010926895081802002
2116	85-89	-0.06288582715514224
2116	90-94	0.19510303666201878
2116	95-99	0.28164381839774677
2116	100-104	-0.087467714335844
2116	105-109	-0.19108146648472513
2116	110-114	-0.004713024070881033
2116	115-119	0.06846984276584323
2116	120-124	0.0601383413547012
2116	125-129	0.03483585031997194
2116	130-134	0.052337375219003945
2116	135-139	0.11110981338907067
2116	140-144	0.08758614397700626
2116	145-149	0.14942168447783644
2116	150-154	0.16011940786577838
2116	155-159	0.136156904360341
2116	160-164	0.06535933113275405
2116	165-169	0.12225791056531676
2116	170-174	0.22271479975649555
2116	175-179	0.3425716054026111
2116	180-184	0.12604782019998595
2116	185-189	-0.017651295464446548
2116	190-194	0.05873638642916035
2116	195-199	0.3323949936077142
2116	200-204	0.23485740587482695
2116	205-209	0.31323962904446745
2116	210-214	0.4692063358712204
2116	215-219	1.0299962912847462
2116	220-224	0.5486047938676251
2116	225-229	0.5926979861475772
2116	230-234	0.7159721397160581
2116	235-239	0.6144755071748023
2116	240-244	0.7812490709090483
2116	245-249	1.5306118803236224
2116	250-254	1.630062429846653
2116	255-259	0.7445757598221014
2116	260-264	1.096000896895955
2116	265-269	1.22018331032195
2116	270-274	0.8069031163019957
2116	275-279	0.12350472066043139
2116	280-284	1.3572521004381919
2116	285-289	0.977320271951232
2116	290-294	0.9852482232263888
2116	295-299	1.1093337083409764
2116	300	0.09792159387516719
1117	1	-3.150536406764104E-4
1117	2	0.0612451000934513
1117	3	0.035474869788323815
1117	4	0.014437123921702266
1117	5	0.02186886729414539
1117	6	-0.030928730492867373
1117	7	-0.0938607041190096
1117	8	0.017631689308778675
1117	9	0.026858372009719744
1117	10-14	0.26390348245205075
1117	15-19	0.16261826727937034
1117	20-24	0.075564155698153
1117	25-29	0.0904456325769587
1117	30-34	0.09926817873796523
1117	35-39	0.03965885981364181
1117	40-44	0.0996471458350996
1117	45-49	0.09024330984406959
1117	50-54	0.08070488526306718
1117	55-59	0.03269430901480774
1117	60-64	-0.05173165931481094
1117	65-69	0.006299663968661662
1117	70-74	0.15620568037153504
1117	75-79	0.15603070822423604
1117	80-84	0.2109638369671032
1117	85-89	0.10121020694910499
1117	90-94	0.35584234833165596
1117	95-99	0.4906263451777093
1117	100-104	0.06395621351911274
1117	105-109	0.037154367574096625
1117	110-114	0.13295588845419815
1117	115-119	0.3144904623956535
1117	120-124	0.26616880238653806
1117	125-129	0.1504069118665754
1117	130-134	0.19915547961356594
1117	135-139	0.2692014093995141
1117	140-144	0.3682715645076797
1117	145-149	0.34816430342631577
1117	150-154	0.3371398524321876
1117	155-159	0.23508606046011238
1117	160-164	0.16715219327742403
1117	165-169	0.2726538140817283
1117	170-174	0.4108541360296343
1117	175-179	0.34323980347975436
1117	180-184	0.2249453939158741
1117	185-189	-0.4860386303370774
1117	190-194	-0.04830222687944996
1117	195-199	-0.04990917195494404
1117	200-204	0.38041718292922155
1117	205-209	0.4591815884297574
1117	210-214	0.4767872908657296
1117	215-219	1.12881757047775
1117	220-224	0.9495451682194158
1117	225-229	0.9987635781457627
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2108	195-199	-0.14609135201231993
2108	200-204	0.1022149890094255
2108	205-209	-0.25539302072714065
2108	210-214	-0.1321497554770943
2108	215-219	0.5672547876941039
2108	220-224	0.28203821294496123
2108	225-229	0.35336422746515694
2108	230-234	0.5064726971440194
2108	235-239	0.26001102719470737
2108	240-244	0.5045836974507125
2108	245-249	0.8166505625749885
2108	250-254	0.5514772242724995
2108	255-259	0.3654926383971997
2108	260-264	0.2774736956990935
2108	265-269	0.4910784617713553
2108	270-274	0.6625735283696557
2108	275-279	0.7252754967263932
2108	280-284	0.2539283711686018
2108	285-289	0.2062925513259053
2108	290-294	0.010433792492403171
2108	295-299	0.6220601653608036
2108	300	0.07185088173414833
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	8.0
15	16.0
16	49.0
17	257.0
18	3812.0
19	7458.0
20	6981.0
21	4589.0
22	2885.0
23	2455.0
24	2703.0
25	3525.0
26	5109.0
27	7255.0
28	10327.0
29	13946.0
30	19246.0
31	26026.0
32	34488.0
33	47159.0
34	69070.0
35	115693.0
36	196122.0
37	108276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.518713115982194	29.441464872814233	13.01023042828736	23.02959158291622
2	27.954394371155228	26.82647256054543	22.137611115717444	23.081521952581898
3	37.64835430185654	22.38562590641769	25.862633262492746	14.103386529233017
4	38.53393438735983	21.28417840191197	21.37014716514003	18.811740045588174
5	40.90454848943719	22.360024379635732	21.477062632098786	15.258364498828294
6	29.606711091134162	23.372012003624942	32.026491761690515	14.994785143550379
7	16.349484114689023	37.18970876808653	32.07042165423684	14.390385462987613
8	25.778700984349612	35.303923579442554	22.395081015740576	16.522294420467258
9	18.65042133421775	23.100286708007314	32.09733235000196	26.151959607772973
10-14	22.806276447031752	34.41479419136269	25.98642246301234	16.792506898593228
15-19	23.571791190456448	31.026839418787155	23.770406671525137	21.630962719231256
20-24	20.450237759633666	29.325094296532594	30.070913319921623	20.153754623912118
25-29	17.816102487566532	28.439077821368258	33.309070068178606	20.4357496228866
30-34	17.200443953748515	30.509368558706772	31.38697725973476	20.90321022780995
35-39	19.99205770816878	30.08095028212592	29.582011436318385	20.344980573386923
40-44	19.111830669174452	29.924548227603392	30.465438666160455	20.4981824370617
45-49	20.261775611345552	29.199123147707716	31.03766188238135	19.501439358565385
50-54	19.79288946686275	29.822578510136605	31.193423898734324	19.19110812426632
55-59	20.07168427498949	30.946776462305387	28.972375079822942	20.00916418288218
60-64	18.779854507687006	28.985961344603822	30.594086338239805	21.640097809469363
65-69	18.265089263505168	32.65681298812002	29.077399524917126	20.00069822345769
70-74	19.068133517780698	33.83864346819229	26.186928965945604	20.906294048081413
75-79	18.67384091269443	35.484065231526536	26.631930049646595	19.21016380613244
80-84	20.465220471329925	32.12843238651323	26.354764429587803	21.05158271256904
85-89	20.33686372569128	32.13253444932716	26.51721775407697	21.013384070904593
90-94	19.17670726545147	31.410833227872498	28.517104292765094	20.89535521391094
95-99	19.262356009594754	30.766198420560354	28.18550633565056	21.78593923419433
100-104	19.56294120813023	29.63629831015377	28.807070676214874	21.99368980550113
105-109	20.08992536282164	29.834768328002102	29.713393816940354	20.361912492235902
110-114	18.988512475220336	29.179930491834565	30.01881712765914	21.81273990528596
115-119	18.07150680986066	28.994310933452034	30.6849426656717	22.24923959101561
120-124	19.43077332611836	29.946629544452836	28.961930820601665	21.660666308827146
125-129	21.076631479113665	30.26013187695557	29.40061880053938	19.26261784339139
130-134	21.151871210318347	29.67351643473034	29.231948154565774	19.942664200385536
135-139	20.041579219001925	28.60558334550376	30.500009600575336	20.85282783491898
140-144	18.29037609235637	30.174055521581806	30.736852884856518	20.798715501205308
145-149	18.69488033319233	31.43306915265322	28.71072419176247	21.161326322391975
150-154	19.511679969278166	28.225595999179586	30.329837852148273	21.93288617939397
155-159	19.18066386504504	29.494908059971575	31.182717805716415	20.141710269266966
160-164	21.402998986993836	28.569392085751176	28.148334125623236	21.879274801631748
165-169	19.504325204652265	28.6705472386127	30.06027415752042	21.764853399214616
170-174	18.051089025262314	31.302006752986504	31.186567107730674	19.460337114020508
175-179	18.940184069159034	31.546375856596537	28.900894453341948	20.612545620902484
180-184	21.58319259766764	28.90403645890155	28.975749826535107	20.537021116895698
185-189	19.001394992283178	29.98893024893121	28.665040387863133	22.344634370922485
190-194	19.450422178150074	29.95016718097643	28.645070408603512	21.954340232269985
195-199	18.83813255427089	31.74895717386991	29.945910940408353	19.466999331450847
200-204	19.652051976917896	28.874769258966715	29.282677221477936	22.190501542637453
205-209	21.257907744316388	28.886057204866034	29.111059714106585	20.74497533671099
210-214	20.633701976031148	29.099372936967278	28.443944568023564	21.82298051897801
215-219	19.48442583031885	29.57236128186882	28.601394003538832	22.3418188842735
220-224	20.374486441951138	28.870821058658354	29.50667007244652	21.248022426943987
225-229	19.40133721468108	29.446157355175323	29.536373670683975	21.616131759459623
230-234	17.787475325801246	30.12962227565571	28.214948091449816	23.86795430709322
235-239	20.057754733810956	30.511966680766907	26.28017853579007	23.150100049632066
240-244	17.517326129216762	30.642243578815197	29.02192829592115	22.81850199604689
245-249	21.263493531977907	27.91436289291434	27.925621746169586	22.896521828938162
250-254	19.80557385967745	30.802098743343237	26.462989065529722	22.929338331449582
255-259	20.042737094139433	29.537412412958446	27.558152558916245	22.86169793398588
260-264	18.365807997276928	30.84745417544901	27.67879975387623	23.107938073397833
265-269	19.3295600173974	31.03713821478808	27.109340338842024	22.52396142897249
270-274	19.104790249309413	29.74082818029875	28.012230547567203	23.142151022824635
275-279	18.929390698208913	29.576920223606063	26.542644724994506	24.951044353190518
280-284	19.35780897478977	29.276887785307927	27.316479964623348	24.048823275278963
285-289	19.6518483284094	30.005571241339485	27.27874680526402	23.06383362498709
290-294	17.62016352975232	31.98657665402591	25.333874456513044	25.059385359708724
295-299	18.892733966680193	29.99314868232142	26.457636019020768	24.656481331977616
300	13.982070203459408	32.37080320426382	30.5600770373215	23.08704955495528
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.25
22	0.25
23	0.5
24	0.5
25	1.75
26	1.5
27	3.75
28	6.5
29	16.25
30	28.5
31	61.25
32	215.0
33	734.75
34	3534.5
35	7259.5
36	10610.25
37	12251.5
38	15127.0
39	15457.25
40	19493.0
41	26028.25
42	27239.5
43	23542.5
44	24390.25
45	19064.25
46	12677.5
47	5959.5
48	3526.25
49	1378.25
50	435.75
51	183.5
52	105.75
53	71.0
54	45.0
55	34.75
56	23.5
57	15.75
58	13.25
59	8.25
60	6.75
61	3.75
62	2.5
63	2.5
64	2.0
65	3.0
66	2.5
67	1.5
68	0.25
69	0.75
70	1.25
71	1.25
72	1.0
73	0.0
74	0.5
75	0.25
76	0.75
77	0.75
78	0.5
79	0.5
80	0.25
81	0.0
82	0.0
83	0.25
84	0.5
85	0.25
86	0.0
87	0.0
88	0.0
89	0.25
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	2.909264407041002E-5
115-119	0.0
120-124	0.0
125-129	0.0
130-134	2.909264407041002E-5
135-139	2.909264407041002E-5
140-144	2.909264407041002E-5
145-149	2.909264407041002E-5
150-154	0.0
155-159	0.0
160-164	2.909264407041002E-5
165-169	2.909264407041002E-5
170-174	2.909264407041002E-5
175-179	0.0
180-184	0.0
185-189	0.0
190-194	5.818528814082004E-5
195-199	2.909264407041002E-5
200-204	0.0
205-209	0.0
210-214	2.909264407041002E-5
215-219	2.909264407041002E-5
220-224	2.909264407041002E-5
225-229	2.909264407041002E-5
230-234	0.0
235-239	2.909264407041002E-5
240-244	2.909264407041002E-5
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	687459.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.774499131471583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.562003427298876	4.699797525527119
2	4.3015073178612555	0.7548714444918448
3	2.422436140764193	0.6376699143954236
4	1.7223138589136044	0.6044976583663556
5	1.64384719257734	0.7211967881770934
6	1.4436355837468973	0.7600307505449173
7	1.4989042040394136	0.9206493545552056
8	1.4864680225944	1.0434409898571877
9	1.5212519192944893	1.2013401280139078
>10	28.52477415422917	55.19207251737939
>50	1.7108610662856496	9.106225927124479
>100	0.09916868444908979	1.6067544504699673
>500	0.013227181202589457	0.8691548750537983
>1k	0.023147248483310096	5.654167294420182
>5k	0.023147248477281574	11.95348473801036
>10k+	0.0033067497824687967	4.274645643612783
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT	19392	2.820822769066955	TruSeq Adapter, Index 8 (97% over 36bp)
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT	10073	1.4652510186062004	No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	6947	1.0105329917856918	No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA	6840	0.9949684272080227	No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT	6578	0.9568570634757854	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	6361	0.9252915446593907	No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCT	6063	0.8819435049944797	No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	6022	0.8759795129600456	No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	5946	0.8649243082132898	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC	5838	0.8492142804152685	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA	5754	0.8369953699056962	No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC	5339	0.7766281334595954	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT	5268	0.7663002448145998	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA	5193	0.755390503288196	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG	5179	0.7533540182032674	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG	5067	0.7370621375238378	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG	4925	0.7164063602338466	No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	4816	0.7005508692154733	No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT	4449	0.6471658673462708	No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC	3513	0.5110122930967519	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG	3081	0.4481721819046663	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT	3060	0.4451174542772733	No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTACTTTCT	3005	0.4371169771579105	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT	2928	0.4259163091908026	No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA	2050	0.2981996017217027	No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCT	1942	0.2824895739236813	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA	1594	0.23186837324116782	No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT	1430	0.2080124051034316	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT	1167	0.16975557815084244	No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTA	1014	0.14749970543697877	No Hit
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT	946	0.1376082064530394	No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA	892	0.12975319255402867	No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT	881	0.12815309713015613	No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT	866	0.12597114882487537	No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA	773	0.11244306933213472	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.007127697797250455	0.0	0.0
2	0.007709550678658654	0.0	0.0
3	0.012800763390980407	0.0	0.0
4	0.01687373356083781	0.0	0.0
5	0.02065577728999111	0.0	0.0
6	0.025165137120904665	0.0	0.0
7	0.03200190847745102	0.0	0.0
8	0.04131155457998222	0.0	0.0
9	0.05556695017448313	0.0	0.0
10-14	0.11523596316289408	0.0	0.0
15-19	0.193349712491945	0.0	0.0
20-24	0.2547351914805101	0.0	0.0
25-29	0.2999451603659272	0.0	0.0
30-34	0.34029665769158596	0.0	0.0
35-39	0.37145487949099515	0.0	0.0
40-44	0.40060570884954594	0.0	0.0
45-49	0.4310947998353356	0.0	0.0
50-54	0.4618748172618294	0.0	0.0
55-59	0.49088018340002826	0.0	0.0
60-64	0.5195946230975229	0.0	0.0
65-69	0.5522947550326637	0.0	0.0
70-74	0.5937808654770684	0.0	0.0
75-79	0.6421619325661603	0.0	0.0
80-84	0.6903102585026889	0.0	0.0
85-89	0.7481464349146639	0.0	0.0
90-94	0.81456494132741	0.0	0.0
95-99	0.8942787860803335	0.0	2.9092644070410017E-5
100-104	0.9889462498854478	0.0	1.4546322035205008E-4
105-109	1.0928069892168115	0.0	1.4546322035205008E-4
110-114	1.2106612903460425	0.0	1.4546322035205008E-4
115-119	1.3518769846638128	0.0	1.4546322035205008E-4
120-124	1.5187232984076142	0.0	1.4546322035205008E-4
125-129	1.7018324001867746	0.0	1.4546322035205008E-4
130-134	1.9050445190185887	0.0	1.4546322035205008E-4
135-139	2.141189510938107	0.0	1.4546322035205008E-4
140-144	2.4178023707595653	0.0	1.4546322035205008E-4
145-149	2.7333411883472323	0.0	1.4546322035205008E-4
150-154	3.0913261736336275	0.0	1.4546322035205008E-4
155-159	3.519017134112725	0.0	1.4546322035205008E-4
160-164	3.9715823052720234	0.0	1.4546322035205008E-4
165-169	4.471713949486442	0.0	1.4546322035205008E-4
170-174	5.0258415410955415	0.0	1.4546322035205008E-4
175-179	5.629222979115846	0.0	1.4546322035205008E-4
180-184	6.302601318769556	0.0	1.4546322035205008E-4
185-189	7.039459807784899	0.0	1.4546322035205008E-4
190-194	7.843144100229978	0.0	1.4546322035205008E-4
195-199	8.711704988952068	0.0	1.4546322035205008E-4
200-204	9.674875156191135	0.0	1.4546322035205008E-4
205-209	10.694135941197947	0.0	1.4546322035205008E-4
210-214	11.776673227057906	0.0	1.4546322035205008E-4
215-219	12.952539715095735	0.0	1.4546322035205008E-4
220-224	14.190577183512033	0.0	1.4546322035205008E-4
225-229	15.471933598949173	0.0	1.4546322035205008E-4
230-234	16.807780536730192	0.0	1.4546322035205008E-4
235-239	18.146856758003025	0.0	1.4546322035205008E-4
240-244	19.487067592394602	0.0	1.4546322035205008E-4
245-249	20.901755595606428	0.0	1.4546322035205008E-4
250-254	22.32383313041214	0.0	1.4546322035205008E-4
255-259	23.768341093796135	0.0	1.4546322035205008E-4
260-264	25.186258380499783	0.0	1.4546322035205008E-4
265-269	26.62427868425608	0.0	1.4546322035205008E-4
270-274	28.107101659880804	0.0	1.4546322035205008E-4
275-279	29.6570122727319	0.0	1.4546322035205008E-4
280-284	31.107542413438473	0.0	1.4546322035205008E-4
285-288	32.345638067142914	0.0	1.4546322035205008E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTT	6905	0.0	287.6133	1
CGGGTTA	7055	0.0	281.49823	2
GGGGGGA	8345	0.0	270.04312	1
GGGTTAT	7480	0.0	265.50403	3
CAGAAGC	8785	0.0	255.01195	9
GGAGCAG	8840	0.0	254.75565	5
GAGCAGA	8895	0.0	252.68465	6
ATTAGTA	7775	0.0	251.83794	8
TATTAGT	7805	0.0	251.62332	7
GGGGAGC	8965	0.0	251.20357	3
TTATTAG	7895	0.0	248.7549	6
GGTTATT	8100	0.0	245.18147	4
GGGAGCA	9745	0.0	231.24783	4
GTTATTA	8570	0.0	229.50525	5
TTAGTAG	8795	0.0	222.4639	9
GGGGGAG	11085	0.0	203.29364	2
CCGGTTA	15	0.0028785486	196.0	1
GCAGAAG	11535	0.0	194.47073	8
AGCAGAA	14250	0.0	157.21263	7
CGGGTAT	20	0.0068058562	147.0	2
>>END_MODULE