FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool f1.3410000000b621.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool f1.3410000000b621.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600706
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT398486.633527882191954TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC124612.0743924648663405No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA94521.5734818696666923No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG79331.3206127456692625No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT54310.9041028389927852No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT53350.8881216435327765No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG50650.8431745313015019No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT46460.7734232719500055No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT42940.7148255552633068No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT40750.6783684531201619No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT36640.6099489600569996No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA34680.5773206859928152No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG29200.486094695241932No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA28500.4744417402190089No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTTGGACAGTACGGATGACAA26830.44664111895003544No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT26010.43299051449461134No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT24130.4016940067187609No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA23780.39586752920729945No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA20720.3449274686785216No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA19940.33194274736726453No Hit
GGGGGGAGCGAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCA19780.3292792147905964No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG18830.3134644901166294No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT16210.26984914417368894No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG14030.2335585128165858No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA13980.23272615888637704No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA13660.2273990937330408No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGACCTCGTAT12180.20276141739886067TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT11520.19177434552010467No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA11240.18711316351093546No Hit
GGGGGGAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCA11070.18428316014822557No Hit
GGGGGGAGCGAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAA11000.18311786464593327No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAACTCGTAT10940.18211903992968276TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG6400.10654130306672481No Hit
GGGGGGAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAA6260.10421071206214022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA54650.0290.50321
GGGTTAT55250.0287.61452
GGGGGGA81350.0266.17211
TTATTAG59850.0266.000035
GGGGAGC81150.0265.197783
GGTTATT60500.0262.899173
GGGAGCG52600.0255.712924
GTTATTA62500.0254.721624
GGAGCGA52850.0252.834435
TATTAGT65600.0243.35676
ATTAGTA65850.0241.763097
TTAGTAG66700.0238.682168
GGGGGAG91300.0237.003282
CGAAAGC59200.0224.224679
TAGTAGA71150.0223.754049
GAGCGAA60250.0221.536936
AGCGAAA60350.0220.926247
GGGAGCA36850.0219.80194
GCGAAAG61250.0216.968
GCAAAAG40500.0194.548168
Produced by FastQC (version 0.11.2)