##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE77A l01n01 clinical flu pool e4.3410000000b770.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1946342
Sequences flagged as poor quality	0
Sequence length	300
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.78542208923201	34.0	34.0	34.0	34.0	34.0
2	33.85888554015687	34.0	34.0	34.0	34.0	34.0
3	33.873491914576164	34.0	34.0	34.0	34.0	34.0
4	33.86210439891859	34.0	34.0	34.0	34.0	34.0
5	33.88301182423233	34.0	34.0	34.0	34.0	34.0
6	37.64418123844627	38.0	38.0	38.0	38.0	38.0
7	37.682046629009704	38.0	38.0	38.0	38.0	38.0
8	37.63611328327704	38.0	38.0	38.0	38.0	38.0
9	37.59589116404003	38.0	38.0	38.0	37.0	38.0
10-14	37.433045374348396	38.0	38.0	38.0	37.0	38.0
15-19	37.48569336735271	38.0	38.0	38.0	37.0	38.0
20-24	37.54124074802886	38.0	38.0	38.0	37.0	38.0
25-29	37.481512293317415	38.0	38.0	38.0	37.0	38.0
30-34	37.398425867601894	38.0	38.0	38.0	37.0	38.0
35-39	37.474501192493406	38.0	38.0	38.0	37.0	38.0
40-44	37.441643657692225	38.0	38.0	38.0	37.0	38.0
45-49	37.44044407406304	38.0	38.0	38.0	37.0	38.0
50-54	37.443227757506136	38.0	38.0	38.0	37.0	38.0
55-59	37.384780783644395	38.0	38.0	38.0	37.0	38.0
60-64	37.38784201337689	38.0	38.0	38.0	37.0	38.0
65-69	37.380107812501606	38.0	38.0	38.0	37.0	38.0
70-74	37.37287403755353	38.0	38.0	38.0	37.0	38.0
75-79	37.18237945849188	38.0	38.0	38.0	37.0	38.0
80-84	37.12331584068987	38.0	38.0	38.0	37.0	38.0
85-89	37.147831265008925	38.0	38.0	38.0	37.0	38.0
90-94	36.912235157027894	38.0	38.0	38.0	36.4	38.0
95-99	36.75138172017045	38.0	38.0	38.0	35.2	38.0
100-104	37.09477923201575	38.0	38.0	38.0	37.0	38.0
105-109	37.06099185035312	38.0	38.0	38.0	37.0	38.0
110-114	36.946597052316605	38.0	38.0	38.0	36.6	38.0
115-119	36.88511392139716	38.0	38.0	38.0	36.0	38.0
120-124	36.82956335525822	38.0	38.0	38.0	35.8	38.0
125-129	36.88614734717742	38.0	38.0	38.0	36.0	38.0
130-134	36.92050122743074	38.0	38.0	38.0	36.0	38.0
135-139	36.86951378534708	38.0	38.0	38.0	36.0	38.0
140-144	36.842445983285565	38.0	38.0	38.0	36.0	38.0
145-149	36.78208464904934	38.0	38.0	38.0	35.8	38.0
150-154	36.76960904095991	38.0	38.0	38.0	35.6	38.0
155-159	36.73507276727317	38.0	38.0	38.0	35.2	38.0
160-164	36.70581809363411	38.0	38.0	38.0	35.0	38.0
165-169	36.613539655415124	38.0	38.0	38.0	35.0	38.0
170-174	36.54622198976336	38.0	37.8	38.0	34.2	38.0
175-179	36.46851509138682	38.0	37.2	38.0	34.0	38.0
180-184	36.369219386931995	38.0	37.0	38.0	34.0	38.0
185-189	36.15461177943033	38.0	37.0	38.0	34.0	38.0
190-194	36.01532834414506	38.0	37.0	38.0	32.8	38.0
195-199	35.74677533547547	38.0	37.0	38.0	31.0	38.0
200-204	35.73186582830766	38.0	37.0	38.0	31.0	38.0
205-209	35.473541957168884	38.0	36.8	38.0	29.4	38.0
210-214	35.261393321420385	38.0	36.0	38.0	28.0	38.0
215-219	34.31730908545363	38.0	34.8	38.0	23.2	38.0
220-224	34.3499813496292	38.0	34.8	38.0	23.4	38.0
225-229	33.998279644584564	38.0	34.0	38.0	22.2	38.0
230-234	33.4536952909612	37.8	34.0	38.0	20.6	38.0
235-239	33.19895691507453	37.0	33.8	38.0	19.6	38.0
240-244	32.43260619151208	37.0	30.6	38.0	17.0	38.0
245-249	31.452012339044224	37.0	27.6	38.0	14.4	38.0
250-254	30.60481919416012	37.0	25.4	37.0	12.2	38.0
255-259	30.1615660557086	36.8	24.4	37.0	10.2	37.8
260-264	29.3771573546684	35.0	22.8	37.0	9.8	37.2
265-269	28.23464910072331	33.0	20.6	37.0	9.2	37.0
270-274	27.52637111052426	32.0	19.6	37.0	9.0	37.0
275-279	26.722699505020188	30.6	18.6	37.0	8.8	37.0
280-284	25.37305273173985	28.2	16.2	36.4	8.0	37.0
285-289	24.246881277802157	26.4	14.2	35.4	8.0	37.0
290-294	23.429545270050177	25.0	13.0	33.8	8.0	37.0
295-299	22.128279408243774	22.4	11.6	32.0	8.0	37.0
300	15.410360563559744	13.0	8.0	21.0	7.0	29.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.24855081387335076
1101	2	-0.023300046753533366
1101	3	-0.016776888890497332
1101	4	-0.006700413745349465
1101	5	0.004030107066988364
1101	6	0.039321158482636065
1101	7	-0.029989103382128235
1101	8	-0.0254375047098776
1101	9	0.011135017131422842
1101	10-14	0.2236998469040259
1101	15-19	0.1298795672520754
1101	20-24	0.02724977907158177
1101	25-29	-0.00818666479564456
1101	30-34	0.11298746342898625
1101	35-39	-0.02996861100677961
1101	40-44	0.006804681444535277
1101	45-49	-0.049108306353900844
1101	50-54	0.0029023254467048787
1101	55-59	0.01784388562664674
1101	60-64	-0.015773044215215748
1101	65-69	0.008809342639246154
1101	70-74	-0.005603946014495875
1101	75-79	0.10841917653112887
1101	80-84	0.04640902225049359
1101	85-89	2.718033470472392E-5
1101	90-94	0.22165098679118245
1101	95-99	0.4600394354396329
1101	100-104	0.048372186492912306
1101	105-109	-0.005515061009539579
1101	110-114	0.14536847715844203
1101	115-119	0.13372098045324776
1101	120-124	0.17070515793932373
1101	125-129	0.06880046713836663
1101	130-134	0.04718964960074601
1101	135-139	0.04991180313199095
1101	140-144	0.040944021094709626
1101	145-149	0.014098810164647091
1101	150-154	0.12209607021902258
1101	155-159	0.015182911384108877
1101	160-164	-0.004840249389779672
1101	165-169	-0.05724475962064446
1101	170-174	0.028254564346816835
1101	175-179	-0.05153885227836952
1101	180-184	-0.06497364997726152
1101	185-189	0.09846358105816222
1101	190-194	0.09363494488813728
1101	195-199	0.22693259197471605
1101	200-204	-0.06796836467439249
1101	205-209	0.06370114686310302
1101	210-214	0.09523332333051826
1101	215-219	0.8064838393010874
1101	220-224	0.35565271349140914
1101	225-229	0.12898978726519061
1101	230-234	0.32522581098052683
1101	235-239	-0.5166990833960483
1101	240-244	-0.2582507287413094
1101	245-249	0.01289001390775013
1101	250-254	0.05348795728749067
1101	255-259	-0.26730363974360927
1101	260-264	-1.0217006437073906
1101	265-269	-0.7888989148147303
1101	270-274	-1.3147768093322867
1101	275-279	-1.3375841226239729
1101	280-284	-0.6358054050716966
1101	285-289	-0.26869676578120405
1101	290-294	-0.3971935375310416
1101	295-299	0.18887364614685254
1101	300	-0.6696002996261363
1102	1	-0.03141942326346481
1102	2	-0.024223757652904965
1102	3	-7.00047534884618E-4
1102	4	0.008803507790773324
1102	5	-0.018493433139376236
1102	6	-0.0066640909131052695
1102	7	-0.009288121451888287
1102	8	-0.026508498208777098
1102	9	-0.026617558470292124
1102	10-14	0.21891669661757618
1102	15-19	0.12907434692000663
1102	20-24	0.03841370518901499
1102	25-29	0.05465814133130209
1102	30-34	0.12179397710235662
1102	35-39	8.97761907651784E-4
1102	40-44	-0.03714627177034657
1102	45-49	0.03266631534266651
1102	50-54	0.06865724442630494
1102	55-59	0.07947997405491236
1102	60-64	0.0751388526773269
1102	65-69	0.06762089108467251
1102	70-74	0.05615348808749587
1102	75-79	0.0960587490654845
1102	80-84	0.15971096304377141
1102	85-89	0.011919896096600269
1102	90-94	0.311805703620486
1102	95-99	0.47180716910069975
1102	100-104	0.07486200256198572
1102	105-109	0.019845936528135155
1102	110-114	0.1428280896336105
1102	115-119	0.20276445627585815
1102	120-124	0.2806777817770154
1102	125-129	0.17243903374058078
1102	130-134	0.12480378978179374
1102	135-139	0.143813178993895
1102	140-144	0.17852690406726168
1102	145-149	0.19159095478841692
1102	150-154	0.21073008142959537
1102	155-159	0.16093179301849148
1102	160-164	0.22268975277674485
1102	165-169	0.1940431964086784
1102	170-174	0.17380893364175876
1102	175-179	0.22621862738849785
1102	180-184	0.19163557452837665
1102	185-189	0.3099924204442601
1102	190-194	0.2861992400956197
1102	195-199	0.4638465987251834
1102	200-204	0.2637719847920934
1102	205-209	0.1969952510829529
1102	210-214	0.445068789608456
1102	215-219	1.2306089773739544
1102	220-224	0.863615050329571
1102	225-229	0.7749325678657399
1102	230-234	0.7823135104299723
1102	235-239	0.7451075447006446
1102	240-244	0.933650984621913
1102	245-249	0.955564427867948
1102	250-254	1.1298657633696827
1102	255-259	0.7619103786682544
1102	260-264	0.9472815166968651
1102	265-269	0.8919527700010903
1102	270-274	-0.3093526401919888
1102	275-279	-0.1013566265764041
1102	280-284	0.20039588387376028
1102	285-289	0.20494102169095996
1102	290-294	0.49875016678041106
1102	295-299	0.6493401851393905
1102	300	-0.30348208113367825
1103	1	-0.1263696426286458
1103	2	-0.053916585113135795
1103	3	-0.021057058716529298
1103	4	0.0291197316203764
1103	5	-0.04686789517864298
1103	6	-0.024025639559972944
1103	7	-0.0763141448769602
1103	8	-0.034110852025705185
1103	9	0.013734229887269578
1103	10-14	0.2179446369752327
1103	15-19	0.08969280137997515
1103	20-24	0.02983292919468994
1103	25-29	0.006768244708631244
1103	30-34	0.05409366359427992
1103	35-39	-0.03260019715600038
1103	40-44	-0.014038770246656895
1103	45-49	0.0013850065808753698
1103	50-54	0.03831286273082668
1103	55-59	0.0647434745772415
1103	60-64	0.01541494000137078
1103	65-69	0.016806015973870103
1103	70-74	0.05235932310126401
1103	75-79	0.04231684515419687
1103	80-84	0.03852081000071905
1103	85-89	-0.028426916058094776
1103	90-94	0.2501938837815061
1103	95-99	0.3520328041484646
1103	100-104	-0.0037204640279568935
1103	105-109	-0.14907858125993556
1103	110-114	0.06517264877960827
1103	115-119	0.16589478412372216
1103	120-124	0.20610459514875856
1103	125-129	0.12405672652047173
1103	130-134	0.07799538548589169
1103	135-139	0.05997848349190349
1103	140-144	0.1295510934478159
1103	145-149	0.15012621895656508
1103	150-154	0.13298811885458406
1103	155-159	0.05871901715023853
1103	160-164	0.027767756216157125
1103	165-169	0.040896284379691394
1103	170-174	0.08827485756028608
1103	175-179	0.05014734102034879
1103	180-184	0.08770359353047041
1103	185-189	0.13607438521843562
1103	190-194	0.10156430988030962
1103	195-199	0.38149276866514015
1103	200-204	0.13740034955710456
1103	205-209	0.07417381555641356
1103	210-214	0.3268841390637647
1103	215-219	1.1285162797619748
1103	220-224	0.6668804171468565
1103	225-229	0.7417562670538373
1103	230-234	0.825624526977009
1103	235-239	0.7066317483458278
1103	240-244	0.8286796266095848
1103	245-249	1.3227299090138658
1103	250-254	1.0452476953315681
1103	255-259	1.03455328014768
1103	260-264	1.234822272126781
1103	265-269	1.1443282903225729
1103	270-274	0.7797353932877868
1103	275-279	-0.015098184634279477
1103	280-284	0.026312254917254307
1103	285-289	0.5381546221349893
1103	290-294	0.5848720331133279
1103	295-299	0.9137786414395421
1103	300	-0.0795580956242059
2116	1	-0.03583475144156267
2116	2	-0.07084137162971871
2116	3	0.01522784647264075
2116	4	0.03724603870002596
2116	5	0.007318489357388103
2116	6	-0.02145637914084375
2116	7	-0.0927141379780636
2116	8	-0.07013885648337492
2116	9	0.013283270573658967
2116	10-14	0.21361077044783627
2116	15-19	0.09077767093208422
2116	20-24	0.01455057178824859
2116	25-29	9.075092031025633E-4
2116	30-34	0.050514076416575904
2116	35-39	-0.0013337691135291152
2116	40-44	0.008606257267025796
2116	45-49	0.05439515095980596
2116	50-54	0.027204504027110943
2116	55-59	0.05210970043648189
2116	60-64	0.02247306070942301
2116	65-69	0.02576502130630587
2116	70-74	0.0023573651597246226
2116	75-79	-0.002986790719724297
2116	80-84	0.060927709880701286
2116	85-89	-0.025303104404763133
2116	90-94	0.19967594768112207
2116	95-99	0.3310546339129772
2116	100-104	-0.08802442955198586
2116	105-109	-0.10278941560444821
2116	110-114	-0.006994075130783983
2116	115-119	0.12779985147528095
2116	120-124	0.14480897009409688
2116	125-129	0.05257709365023544
2116	130-134	-0.006823668716627651
2116	135-139	0.049614598159237744
2116	140-144	0.1184715452300793
2116	145-149	0.12477983234219181
2116	150-154	0.1718782903388032
2116	155-159	0.18427646009956788
2116	160-164	0.02557397221590918
2116	165-169	0.12233785146765541
2116	170-174	0.1652201592544884
2116	175-179	0.2296516668779347
2116	180-184	0.2269121085910939
2116	185-189	-3.788017011316924E-4
2116	190-194	0.03934844868812348
2116	195-199	0.20575852341436018
2116	200-204	0.262535607521059
2116	205-209	0.30300135352140245
2116	210-214	0.31259061625617335
2116	215-219	1.0396606407258346
2116	220-224	0.5880317721020631
2116	225-229	0.5974420985391689
2116	230-234	0.7117921635810376
2116	235-239	0.49973772591783927
2116	240-244	0.6338627444024212
2116	245-249	1.2171654102434672
2116	250-254	1.3566027040479298
2116	255-259	0.608836537050184
2116	260-264	0.7379142251967892
2116	265-269	0.9736328934883147
2116	270-274	0.5762523255770304
2116	275-279	-0.3127580883454968
2116	280-284	0.9271792994057719
2116	285-289	0.37106248856555624
2116	290-294	0.5379946316745325
2116	295-299	0.7229258188742769
2116	300	0.037957410712310846
1117	1	-0.012724230168728923
1117	2	0.01558670241251292
1117	3	-0.004458042193434153
1117	4	0.0289488306072343
1117	5	-0.023594135090142743
1117	6	-0.08446021819567306
1117	7	-0.05091007444573137
1117	8	0.01078783854752885
1117	9	0.002220869645796597
1117	10-14	0.23099379113754992
1117	15-19	0.09611748900998407
1117	20-24	0.07381165377138643
1117	25-29	0.015389762614184122
1117	30-34	0.08618058443384058
1117	35-39	0.023728389693836505
1117	40-44	0.06665496955062622
1117	45-49	0.049795216338864634
1117	50-54	0.018078270415735176
1117	55-59	0.023119627195939074
1117	60-64	-0.08097317298597062
1117	65-69	-0.03637684559050314
1117	70-74	0.11538407457084077
1117	75-79	0.057850790495756144
1117	80-84	0.10563842893477471
1117	85-89	0.03896657654987479
1117	90-94	0.25269341334725226
1117	95-99	0.3768171612135589
1117	100-104	-0.012096505268850422
1117	105-109	-0.08032053900699765
1117	110-114	-0.026713077016360387
1117	115-119	0.14330326964385165
1117	120-124	0.16355525305010588
1117	125-129	0.07435085337203162
1117	130-134	0.07466533542393705
1117	135-139	0.21405041375640366
1117	140-144	0.2382599059841226
1117	145-149	0.22538671610396221
1117	150-154	0.20753465145743633
1117	155-159	0.1237699096826077
1117	160-164	0.12169099456541943
1117	165-169	0.10466676315049028
1117	170-174	0.2091445479844296
1117	175-179	0.18729637543145117
1117	180-184	0.09280590170567393
1117	185-189	-0.7693826192454054
1117	190-194	-0.13564906438016777
1117	195-199	-0.27584645666320995
1117	200-204	0.15247226327926455
1117	205-209	0.2021128190859116
1117	210-214	0.29608332868622256
1117	215-219	1.0580264113619648
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2108	220-224	0.6319514459781175
2108	225-229	0.5173186399272467
2108	230-234	0.7226809776478902
2108	235-239	0.4600569394018095
2108	240-244	0.8882140994954497
2108	245-249	1.2581016524442745
2108	250-254	0.7650340535597238
2108	255-259	0.7447184205663895
2108	260-264	0.729439702193833
2108	265-269	0.884595734810194
2108	270-274	1.1510333744345331
2108	275-279	1.3343580309606757
2108	280-284	0.8769580681186859
2108	285-289	0.9322086219716468
2108	290-294	0.264163553572228
2108	295-299	0.9945142635333006
2108	300	-0.027507014282159403
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	9.0
14	24.0
15	34.0
16	54.0
17	237.0
18	3267.0
19	6703.0
20	6227.0
21	4469.0
22	3484.0
23	3902.0
24	5351.0
25	8295.0
26	12779.0
27	19779.0
28	29328.0
29	42444.0
30	60734.0
31	83956.0
32	110193.0
33	147542.0
34	210674.0
35	334232.0
36	556012.0
37	296612.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.599277002705588	27.16876068029154	15.085786567828263	26.146175749174606
2	36.6058483041521	24.29619254992185	21.006585687407455	18.091373458518596
3	40.74890229980137	21.296411422041963	22.054243293316386	15.900442984840279
4	40.70553890323489	21.497969010584985	20.480778814822884	17.315713271357243
5	33.91485155229657	21.379130697482765	28.479681371516413	16.226336378704257
6	28.839844179491582	21.76708923714332	32.93583553147391	16.45723105189119
7	16.96484995956517	41.65737573355556	25.041436705368326	16.336337601510937
8	27.41722677720565	26.92830961876176	28.926570972624543	16.727892631408046
9	17.1793549129598	21.701941385429695	33.62893057849032	27.48977312312019
10-14	24.52357293836335	34.46334714043061	24.461579722371503	16.551500198834532
15-19	23.091543007344033	32.56275618570631	22.925117990568975	21.420582816380676
20-24	23.726210501545978	30.7750847487235	26.998204837587636	18.50049991214288
25-29	19.018004030124203	27.942149940760668	34.07031241169332	18.96953361742181
30-34	18.688709384065085	29.579303123500395	30.717284012778844	21.014703479655683
35-39	21.14724955840238	30.54933819441804	27.69882168704164	20.604590560137943
40-44	19.220167884164244	30.63384543929073	28.993866442793713	21.152120233751315
45-49	21.605349933362174	29.246185922104136	28.027027110343404	21.12143703419029
50-54	22.17394476407538	30.093138821440423	26.994125390090744	20.738791024393453
55-59	21.220371342754767	28.770431917926036	28.406518484418463	21.60267825490073
60-64	20.911494485552897	27.672639238119505	29.574134453246142	21.841731823081453
65-69	19.357091405313145	29.24147965773744	28.48147961663469	22.91994932031472
70-74	19.782268481078866	28.80478353752835	28.524688877905323	22.888259103487467
75-79	20.080355867571065	28.792421886800984	28.880669481519693	22.24655276410826
80-84	21.760964928054783	29.318773370764234	26.595737028744175	22.32452467243681
85-89	20.83589626078048	28.29308518235747	26.95564294455959	23.915375612302466
90-94	21.11763503022593	30.061613015595412	28.26832077815718	20.55243117602148
95-99	22.251310406906903	28.739625410128333	28.066742638241376	20.942321544723384
100-104	21.137539034763677	28.224186705111435	28.09182558871976	22.546448671405127
105-109	22.849684176778798	28.874781513218128	26.510890686220613	21.764643623782458
110-114	21.20327260060154	28.376729269573385	29.26862802118024	21.151370108644834
115-119	22.14678612494618	28.70069083439601	28.118778714121156	21.033744326536652
120-124	20.333374093556014	28.499143521539377	27.254870932241097	23.91261145266351
125-129	20.294552550373986	27.00658979768201	30.09142278181327	22.60743487013074
130-134	22.856920608702954	28.757764951664704	28.315674050672907	20.06964038895943
135-139	22.867543319724902	27.081489275779898	27.499719987545866	22.551247416949334
140-144	20.86377419795699	28.464267841931175	27.963595298256937	22.708362661854906
145-149	21.17234496017092	28.42491488391196	29.006837339669733	21.395902816247382
150-154	21.91863506002542	28.201097237792744	26.528112736610527	23.352154965571312
155-159	20.850158913490024	27.37960748933127	30.68520331986876	21.08503027730995
160-164	22.964371112579393	30.423810409475827	26.080236669608936	20.53158180833584
165-169	21.51798604767302	28.4443946644526	27.90216724501655	22.135452042857835
170-174	20.79573887836773	31.1096199948416	27.253350130655353	20.841290996135314
175-179	19.422444770754574	29.716812358773538	28.177247369681176	22.683495500790716
180-184	21.180871604270987	29.639282304959764	27.05761885629555	22.122227234473694
185-189	20.744689268381407	29.002487743675058	28.082186994885788	22.170635993057747
190-194	20.328731572224363	28.461311368845905	27.295822347338994	23.91413471159074
195-199	20.552739446613185	31.49580084075666	26.738260798975723	21.213198913654434
200-204	21.718721581304827	28.47999991779451	26.723833735283932	23.077444765616733
205-209	20.948959638131427	28.40485382322326	28.292756360392985	22.35343017825233
210-214	22.98658714655492	28.142299760268237	28.27306814526943	20.598044947907407
215-219	21.601650686261717	29.429966573192175	27.548498670839965	21.419884069706146
220-224	20.349753537661933	29.0362844762123	27.59909615062512	23.014865835500647
225-229	21.57902362483058	29.844282248443488	26.79166354114539	21.78503058558054
230-234	20.436357022558212	29.823823356840677	26.5716919225912	23.168127698009908
235-239	21.40471715659427	28.528583363047193	25.939819415087378	24.126880065271163
240-244	21.35593847330017	32.057356826292605	25.08540636743183	21.501298332975395
245-249	21.900128548836744	30.33065103666262	25.35134113120921	22.417879283291427
250-254	20.80539802357448	29.598549484109167	25.276164209578788	24.319888282737566
255-259	21.08456787142239	29.967785723166845	25.648359846316833	23.299286559093932
260-264	18.946916831677065	31.429902863936555	26.89460536740203	22.72857493698435
265-269	20.279334258830154	30.908473433754192	26.031766256906547	22.78042605050911
270-274	18.43403866679532	32.60643120339912	25.543344956163406	23.416185173642162
275-279	19.521625695792412	31.784187979296547	24.527847623901657	24.16633870100938
280-284	17.910315864323948	32.02536861456003	25.806780103393955	24.257535417722064
285-289	20.15459770173998	31.065537300227813	25.2661248639756	23.513740134056604
290-294	18.694515146875524	32.93633904010703	25.182028646558518	23.187117166458926
295-299	18.472837764380568	32.090978872161216	24.432119329490913	25.00406403396731
300	15.406696253793012	36.440358374838546	24.128390591170515	24.024554780197928
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.25
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	0.5
23	0.25
24	1.5
25	3.5
26	2.25
27	4.75
28	9.25
29	17.0
30	34.25
31	71.5
32	223.5
33	679.5
34	2312.5
35	4759.5
36	9268.75
37	19449.75
38	30050.0
39	34524.0
40	47519.25
41	50474.5
42	58334.0
43	70871.0
44	68191.5
45	71100.25
46	55902.25
47	40278.25
48	32419.75
49	23611.25
50	18529.5
51	9182.5
52	1490.5
53	191.75
54	81.0
55	55.25
56	45.0
57	29.75
58	21.75
59	18.0
60	13.0
61	9.75
62	7.0
63	5.0
64	3.25
65	2.5
66	1.75
67	1.0
68	1.5
69	1.25
70	0.5
71	0.75
72	0.5
73	0.5
74	0.5
75	0.25
76	0.0
77	0.25
78	0.0
79	0.25
80	0.0
81	0.0
82	0.5
83	0.25
84	0.0
85	0.0
86	0.25
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	1.0275686390161647E-5
135-139	0.0
140-144	0.0
145-149	1.0275686390161647E-5
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	1.0275686390161647E-5
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	1.0275686390161647E-5
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	1946342.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.61904733571254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	45.72117212981014	3.483517747012844
2	4.083744118607855	0.6222847949322187
3	2.9254651309006015	0.6686777193392448
4	2.464548784161073	0.7511005539078399
5	2.44818049637352	0.9326401544119034
6	2.5182541586534453	1.1512018582881332
7	2.604492016306152	1.3890623570405376
8	2.241019247132095	1.3659545379313847
9	2.1697865300713275	1.487853565290441
>10	29.43037547698455	46.46984610173915
>50	2.9487234659369617	14.874026840632732
>100	0.38028999155669674	4.147475420111953
>500	0.01315486496845773	0.7048053456101939
>1k	0.03047851108524746	5.713679954188025
>5k	0.01286621571950106	6.477658906174505
>10k+	0.007448861732342559	9.760214143389025
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	35790	1.8388340795194267	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	28345	1.4563216536456596	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	18500	0.9505009910899522	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT	18039	0.9268155339606298	TruSeq Adapter, Index 1 (97% over 36bp)
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	15240	0.7830073029303174	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	14543	0.7471965358606041	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA	13307	0.6836927939694051	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC	12942	0.6649396663073601	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	11442	0.5878720183811478	No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG	10784	0.554065010157516	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTTAATTGTCGTACTCTTC	10242	0.5262179000401779	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC	9385	0.4821865838583353	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	9323	0.4790011210773852	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG	9136	0.46939335430258405	No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA	8472	0.43527807548724734	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG	8338	0.42839336560583907	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	6898	0.3544084235966752	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG	6592	0.3386866234197279	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	6327	0.3250713389527637	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	6051	0.3108908917343406	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	6032	0.3099147015272753	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA	6028	0.30970918779947204	No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA	5873	0.30174553084709677	No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA	5547	0.2849961620311333	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	5531	0.2841741071199203	No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	5412	0.27806007371777414	No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	5242	0.26932574028613676	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA	5193	0.26680819712054715	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	5123	0.2632117068839906	No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT	5048	0.25935832448768	No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG	4945	0.2540663459967467	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4891	0.25129191067140305	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAACATTAATTG	4567	0.23464529871934123	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT	4200	0.2157894141933946	No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA	4116	0.21147362590952667	No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT	3930	0.20191723756667634	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	3869	0.1987831532176771	No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAGTCATTTGTGT	3766	0.19349117472674382	No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	3582	0.1840375432477951	No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC	3269	0.1679560940471921	No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT	3250	0.16697990384012676	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3130	0.16081449200602976	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTTTAT	2948	0.15146361739098269	No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG	2842	0.146017503604197	No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGT	2733	0.1404172545215589	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	2577	0.13240221913723282	No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT	2508	0.12885710733262704	No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT	2496	0.12824056614921733	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT	2496	0.12824056614921733	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2467	0.1267505916226439	No Hit
GGGGGGAGCGAAAGCAGGCCTACCAGAAGCGAATGGGAGTGCAGATGCAG	2442	0.1254661308238737	No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	2388	0.12269169549853007	No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA	2311	0.11873555623831782	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACA	2245	0.11534457972956448	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	2230	0.11457390325030237	No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTG	2191	0.11257014440422083	No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT	2082	0.10696989532158274	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	3.5964902365565764E-4	0.0	0.0
2	3.5964902365565764E-4	0.0	0.0
3	4.624058875572741E-4	0.0	0.0
4	6.165411834096989E-4	0.0	0.0
5	7.706764792621236E-4	0.0	0.0
6	9.248117751145482E-4	0.0	0.0
7	0.001335839230721014	0.0	0.0
8	0.001335839230721014	0.0	0.0
9	0.0015927313904750553	0.0	0.0
10-14	0.0021681698283241074	0.0	0.0
15-19	0.003504009059045121	0.0	0.0
20-24	0.005076189076739854	0.0	0.0
25-29	0.006381201248290383	0.0	0.0
30-34	0.009638593833971625	0.0	0.0
35-39	0.010851124828010698	0.0	0.0
40-44	0.012197239745121874	0.0	0.0
45-49	0.014385960946226305	0.0	0.0
50-54	0.017057639407668333	0.0	0.0
55-59	0.021137086904562508	0.0	0.0
60-64	0.026696233241639954	0.0	0.0
65-69	0.034906506667379114	0.0	0.0
70-74	0.04520274443032109	0.0	0.0
75-79	0.05794459555412153	0.0	0.0
80-84	0.0762764200741699	0.0	0.0
85-89	0.10199646310874451	0.0	0.0
90-94	0.13746813252758253	0.0	0.0
95-99	0.18390395932472298	0.0	0.0
100-104	0.24558890472486333	0.0	0.0
105-109	0.3241157001184787	0.0	0.0
110-114	0.42606078479527243	0.0	0.0
115-119	0.5538697721161029	0.0	0.0
120-124	0.7123722346843463	0.0	0.0
125-129	0.9133338334167377	0.0	0.0
130-134	1.1569600820410801	5.137843195080823E-5	5.137843195080823E-5
135-139	1.4569792975746296	5.137843195080823E-5	5.137843195080823E-5
140-144	1.8308704225670513	5.137843195080823E-5	5.137843195080823E-5
145-149	2.2682755651370625	5.137843195080823E-5	5.137843195080823E-5
150-154	2.7819776791540236	5.137843195080823E-5	5.137843195080823E-5
155-159	3.4118875305573226	5.137843195080823E-5	5.137843195080823E-5
160-164	4.161971534293562	5.137843195080823E-5	5.137843195080823E-5
165-169	4.963382591548659	5.137843195080823E-5	5.137843195080823E-5
170-174	5.873099383356059	5.137843195080823E-5	5.137843195080823E-5
175-179	6.874197854231168	5.137843195080823E-5	5.137843195080823E-5
180-184	7.97916296313803	5.137843195080823E-5	5.137843195080823E-5
185-189	9.17753406133146	5.137843195080823E-5	5.137843195080823E-5
190-194	10.48821841176936	5.137843195080823E-5	5.137843195080823E-5
195-199	11.897066394292473	5.137843195080823E-5	5.137843195080823E-5
200-204	13.374833405434398	5.137843195080823E-5	7.192980473113151E-5
205-209	14.910904661154103	5.137843195080823E-5	1.0275686390161647E-4
210-214	16.459532805642585	5.137843195080823E-5	1.0275686390161647E-4
215-219	18.05224364474486	5.137843195080823E-5	1.0275686390161647E-4
220-224	19.697576273851155	5.137843195080823E-5	1.0275686390161647E-4
225-229	21.418589333220986	5.137843195080823E-5	1.0275686390161647E-4
230-234	23.13583121568563	5.137843195080823E-5	1.0275686390161647E-4
235-239	24.81383025182625	5.137843195080823E-5	1.0275686390161647E-4
240-244	26.44902077846545	5.137843195080823E-5	1.0275686390161647E-4
245-249	28.068797775519407	5.137843195080823E-5	1.0275686390161647E-4
250-254	29.702035921744482	5.137843195080823E-5	1.0275686390161647E-4
255-259	31.35646253330607	5.137843195080823E-5	1.0275686390161647E-4
260-264	32.94191873781689	5.137843195080823E-5	1.0275686390161647E-4
265-269	34.544144862516454	5.137843195080823E-5	1.0275686390161647E-4
270-274	36.121236658305676	5.137843195080823E-5	1.541352958524247E-4
275-279	37.72221942495204	5.137843195080823E-5	1.541352958524247E-4
280-284	39.196636562330774	5.137843195080823E-5	1.6441098224258635E-4
285-288	40.4855621468375	5.137843195080823E-5	2.0551372780323293E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTT	8375	0.0	290.6651	1
GGGGGGA	22695	0.0	287.26373	1
GGAGCGA	11235	0.0	261.15887	5
GGGGAGC	25590	0.0	254.47832	3
CGAAAGC	11645	0.0	250.44913	9
GCGAAAG	11955	0.0	244.81555	8
GGGGGAG	26700	0.0	244.5045	2
GGGAGCG	12105	0.0	244.08923	4
GAGCGAA	12280	0.0	238.81514	6
AGCGAAA	12455	0.0	235.34161	7
TAGTAGA	20555	0.0	221.2688	9
GGAGCAG	8375	0.0	217.99883	5
GCGTAAG	105	0.0	196.00002	8
GGGAGCA	18615	0.0	191.34084	4
CGGGTTA	24010	0.0	190.59183	1
GGGTTAT	24955	0.0	183.49228	2
GTTATTA	25745	0.0	177.80463	4
TATTAGT	25770	0.0	177.11873	6
ATTAGTA	25815	0.0	176.75305	7
TTATTAG	26045	0.0	175.70013	5
>>END_MODULE