FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool e3.3410000000b706.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool e3.3410000000b706.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568177
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT524319.227934252882465TruSeq Adapter, Index 8 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC237444.178979437745632No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG145082.5534296530834584No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA96931.7059824667313177No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG84691.4905566399203065No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA74831.3170191683225474No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG66781.1753379668659591No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATACTCCTC65681.1559778026917666No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA52940.9317519012561226No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG40300.709286014745405No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC38140.6712696923669913No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA33300.5860849700005456No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTCAAATT25050.44088373869410413No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG24230.4264516163097063No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC22440.39494734915352087No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT19290.3395068790183341No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG19270.33915487603334876No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCACCTCGTAT14010.2465780909822115TruSeq Adapter, Index 8 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA13360.23513799397018886No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA13210.23249797158279903No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCAACTCGTAT12670.22299389098819558TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA12340.21718584173593794No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA10140.1784655133875535No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA8680.1527692954836257No Hit
GGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGT6720.11827300295506507No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTT108.550414E-4294.01
GGGGGGA60350.0292.294951
CGGGTTA59250.0292.26331
GGGTTAT59700.0289.81412
GGTTATT61500.0281.331733
GGGGGAG62950.0279.98892
ATTAGTA61850.0278.788977
TATTAGT63250.0273.0836
TTATTAG63600.0271.811345
GTTATTA64150.0269.710054
TTAGTAG66000.0260.813668
GGAGCGA41650.0258.70595
GCGAAAG41900.0256.81158
CGAAAGC42200.0254.637449
GAGCGAA42300.0254.3836
GGGGAGC72800.0241.701923
TAGTAGA72600.0237.305799
GGGAGCG49100.0220.050924
AGCGAAA49150.0218.929817
GAGCAAA35500.0187.994376
Produced by FastQC (version 0.11.2)